The cannabinoid receptor CB1 is expressed and functional in isolated nuclei of rat cerebral cortex
- Autores
- Gaveglio, Virginia Lucía; Pascual, Ana Clara; Giusto, Norma Maria; Pasquaré, Susana Juana
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Lipids in nuclei are membrane components as well as signaling molecules with a potential role in regulating gene transcription. Former studies from our lab demonstrated an active nuclear glycerolipid metabolism in the central nervous system. We detected diacylglycerol lipase, monoacylglycerol lipase, and lysophosphate phosphatase enzymatic activities, which are responsible for maintaining the levels of endocannabinoid 2-arachidonoyl-glycerol (2-AG), a bioactive lipid mediator. The endocannabinoid system is a cellular signaling mechanism with a protective role in many pathophysiological processes, especially in the nervous system. 2-AG activates CB1 and CB2 GPCR receptors and triggers different signaling cascades, modulating intracellular Ca2+ levels, ERK1/2 phosphorylation, and other second messengers. Different studies have reported that CB1 is expressed not only in plasma membrane but also in intracellular compartments where they also seem to be functional. Therefore, the aim of this work was to study CB1 expression and function in isolated nuclei from rat cerebral cortex (CC). To this end, CC from Wistar rats were dissected, homogenized and highly purified nuclei (CCN) were isolated on a sucrose-density ultracentrifugation. CB1 protein expression was observed in CCN as well as in isolated cerebellum nuclei by Western Blot, which was confirmed by immunocytochemistry. In order to evaluate if CB1 is functional, CCN were incubated at 37 °C with a CB1 agonist (WIN 55-212-2) and ERK1/2 and Akt signaling cascades were studied by Western Blot. Interestingly, it was observed that ERK1/2 phosphorylation increased in nuclei treated with WIN 5 µM for 30 min with respect to controls (p<0.01) while no changes were seen in Akt phosphorylation. Taken together, these results demonstrate that CB1 has also a nuclear localization in the cerebral cortex where it could have a potential role in chromatin regulation and gene expression.
Fil: Gaveglio, Virginia Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Pascual, Ana Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Pasquaré, Susana Juana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
LXII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; LXV Reunión Anual de la Sociedad Argentina de Inmunología; Reunión de la Sociedad Argentina de Andrología; XLVI Reunión Anual de la Sociedad Argentina de Biofísica; XIX Reunión Anual de la Sociedad Argentina de Biología; XLIX Reunión Anual de la Sociedad Argentina de Farmacología Experimental; Reunión Anual de la Sociedad Argentina de Fisiología; Reunión de la Sociedad Argentina de Hematología y XXIX Reunión Anual de la Sociedad Argentina de Protozoología
Buenos Aires
Argentina
Sociedad Argentina de Investigación Clínica
Sociedad Argentina De Investigación Bioquímica Y Biología Molecular
Sociedad Argentina De Inmunología
Sociedad Argentina De Andrología
Sociedad Argentina de Biofísica
Sociedad Argentina De Biología
Sociedad Argentina De Farmacología Experimental
Sociedad Argentina De Fisiología
Sociedad Argentina De Hematología
Sociedad Argentina De Protozoología - Materia
-
CB1
NUCLEI
CEREBRAL CORTEX - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
- Repositorio
.jpg)
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/244020
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The cannabinoid receptor CB1 is expressed and functional in isolated nuclei of rat cerebral cortexGaveglio, Virginia LucíaPascual, Ana ClaraGiusto, Norma MariaPasquaré, Susana JuanaCB1NUCLEICEREBRAL CORTEXhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Lipids in nuclei are membrane components as well as signaling molecules with a potential role in regulating gene transcription. Former studies from our lab demonstrated an active nuclear glycerolipid metabolism in the central nervous system. We detected diacylglycerol lipase, monoacylglycerol lipase, and lysophosphate phosphatase enzymatic activities, which are responsible for maintaining the levels of endocannabinoid 2-arachidonoyl-glycerol (2-AG), a bioactive lipid mediator. The endocannabinoid system is a cellular signaling mechanism with a protective role in many pathophysiological processes, especially in the nervous system. 2-AG activates CB1 and CB2 GPCR receptors and triggers different signaling cascades, modulating intracellular Ca2+ levels, ERK1/2 phosphorylation, and other second messengers. Different studies have reported that CB1 is expressed not only in plasma membrane but also in intracellular compartments where they also seem to be functional. Therefore, the aim of this work was to study CB1 expression and function in isolated nuclei from rat cerebral cortex (CC). To this end, CC from Wistar rats were dissected, homogenized and highly purified nuclei (CCN) were isolated on a sucrose-density ultracentrifugation. CB1 protein expression was observed in CCN as well as in isolated cerebellum nuclei by Western Blot, which was confirmed by immunocytochemistry. In order to evaluate if CB1 is functional, CCN were incubated at 37 °C with a CB1 agonist (WIN 55-212-2) and ERK1/2 and Akt signaling cascades were studied by Western Blot. Interestingly, it was observed that ERK1/2 phosphorylation increased in nuclei treated with WIN 5 µM for 30 min with respect to controls (p<0.01) while no changes were seen in Akt phosphorylation. Taken together, these results demonstrate that CB1 has also a nuclear localization in the cerebral cortex where it could have a potential role in chromatin regulation and gene expression.Fil: Gaveglio, Virginia Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Pascual, Ana Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Pasquaré, Susana Juana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaLXII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; LXV Reunión Anual de la Sociedad Argentina de Inmunología; Reunión de la Sociedad Argentina de Andrología; XLVI Reunión Anual de la Sociedad Argentina de Biofísica; XIX Reunión Anual de la Sociedad Argentina de Biología; XLIX Reunión Anual de la Sociedad Argentina de Farmacología Experimental; Reunión Anual de la Sociedad Argentina de Fisiología; Reunión de la Sociedad Argentina de Hematología y XXIX Reunión Anual de la Sociedad Argentina de ProtozoologíaBuenos AiresArgentinaSociedad Argentina de Investigación ClínicaSociedad Argentina De Investigación Bioquímica Y Biología MolecularSociedad Argentina De InmunologíaSociedad Argentina De AndrologíaSociedad Argentina de BiofísicaSociedad Argentina De BiologíaSociedad Argentina De Farmacología ExperimentalSociedad Argentina De FisiologíaSociedad Argentina De HematologíaSociedad Argentina De ProtozoologíaFundación Revista Medicina2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/244020The cannabinoid receptor CB1 is expressed and functional in isolated nuclei of rat cerebral cortex; LXII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; LXV Reunión Anual de la Sociedad Argentina de Inmunología; Reunión de la Sociedad Argentina de Andrología; XLVI Reunión Anual de la Sociedad Argentina de Biofísica; XIX Reunión Anual de la Sociedad Argentina de Biología; XLIX Reunión Anual de la Sociedad Argentina de Farmacología Experimental; Reunión Anual de la Sociedad Argentina de Fisiología; Reunión de la Sociedad Argentina de Hematología y XXIX Reunión Anual de la Sociedad Argentina de Protozoología; Buenos Aires; Argentina; 2017; 307-3080076-60461669-9106CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://medicinabuenosaires.com/revistas/vol77-17/Vol.77SuplementoI-2017.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:05:16Zoai:ri.conicet.gov.ar:11336/244020instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:05:16.394CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
| dc.title.none.fl_str_mv |
The cannabinoid receptor CB1 is expressed and functional in isolated nuclei of rat cerebral cortex |
| title |
The cannabinoid receptor CB1 is expressed and functional in isolated nuclei of rat cerebral cortex |
| spellingShingle |
The cannabinoid receptor CB1 is expressed and functional in isolated nuclei of rat cerebral cortex Gaveglio, Virginia Lucía CB1 NUCLEI CEREBRAL CORTEX |
| title_short |
The cannabinoid receptor CB1 is expressed and functional in isolated nuclei of rat cerebral cortex |
| title_full |
The cannabinoid receptor CB1 is expressed and functional in isolated nuclei of rat cerebral cortex |
| title_fullStr |
The cannabinoid receptor CB1 is expressed and functional in isolated nuclei of rat cerebral cortex |
| title_full_unstemmed |
The cannabinoid receptor CB1 is expressed and functional in isolated nuclei of rat cerebral cortex |
| title_sort |
The cannabinoid receptor CB1 is expressed and functional in isolated nuclei of rat cerebral cortex |
| dc.creator.none.fl_str_mv |
Gaveglio, Virginia Lucía Pascual, Ana Clara Giusto, Norma Maria Pasquaré, Susana Juana |
| author |
Gaveglio, Virginia Lucía |
| author_facet |
Gaveglio, Virginia Lucía Pascual, Ana Clara Giusto, Norma Maria Pasquaré, Susana Juana |
| author_role |
author |
| author2 |
Pascual, Ana Clara Giusto, Norma Maria Pasquaré, Susana Juana |
| author2_role |
author author author |
| dc.subject.none.fl_str_mv |
CB1 NUCLEI CEREBRAL CORTEX |
| topic |
CB1 NUCLEI CEREBRAL CORTEX |
| purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
| dc.description.none.fl_txt_mv |
Lipids in nuclei are membrane components as well as signaling molecules with a potential role in regulating gene transcription. Former studies from our lab demonstrated an active nuclear glycerolipid metabolism in the central nervous system. We detected diacylglycerol lipase, monoacylglycerol lipase, and lysophosphate phosphatase enzymatic activities, which are responsible for maintaining the levels of endocannabinoid 2-arachidonoyl-glycerol (2-AG), a bioactive lipid mediator. The endocannabinoid system is a cellular signaling mechanism with a protective role in many pathophysiological processes, especially in the nervous system. 2-AG activates CB1 and CB2 GPCR receptors and triggers different signaling cascades, modulating intracellular Ca2+ levels, ERK1/2 phosphorylation, and other second messengers. Different studies have reported that CB1 is expressed not only in plasma membrane but also in intracellular compartments where they also seem to be functional. Therefore, the aim of this work was to study CB1 expression and function in isolated nuclei from rat cerebral cortex (CC). To this end, CC from Wistar rats were dissected, homogenized and highly purified nuclei (CCN) were isolated on a sucrose-density ultracentrifugation. CB1 protein expression was observed in CCN as well as in isolated cerebellum nuclei by Western Blot, which was confirmed by immunocytochemistry. In order to evaluate if CB1 is functional, CCN were incubated at 37 °C with a CB1 agonist (WIN 55-212-2) and ERK1/2 and Akt signaling cascades were studied by Western Blot. Interestingly, it was observed that ERK1/2 phosphorylation increased in nuclei treated with WIN 5 µM for 30 min with respect to controls (p<0.01) while no changes were seen in Akt phosphorylation. Taken together, these results demonstrate that CB1 has also a nuclear localization in the cerebral cortex where it could have a potential role in chromatin regulation and gene expression. Fil: Gaveglio, Virginia Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina Fil: Pascual, Ana Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina Fil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina Fil: Pasquaré, Susana Juana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina LXII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; LXV Reunión Anual de la Sociedad Argentina de Inmunología; Reunión de la Sociedad Argentina de Andrología; XLVI Reunión Anual de la Sociedad Argentina de Biofísica; XIX Reunión Anual de la Sociedad Argentina de Biología; XLIX Reunión Anual de la Sociedad Argentina de Farmacología Experimental; Reunión Anual de la Sociedad Argentina de Fisiología; Reunión de la Sociedad Argentina de Hematología y XXIX Reunión Anual de la Sociedad Argentina de Protozoología Buenos Aires Argentina Sociedad Argentina de Investigación Clínica Sociedad Argentina De Investigación Bioquímica Y Biología Molecular Sociedad Argentina De Inmunología Sociedad Argentina De Andrología Sociedad Argentina de Biofísica Sociedad Argentina De Biología Sociedad Argentina De Farmacología Experimental Sociedad Argentina De Fisiología Sociedad Argentina De Hematología Sociedad Argentina De Protozoología |
| description |
Lipids in nuclei are membrane components as well as signaling molecules with a potential role in regulating gene transcription. Former studies from our lab demonstrated an active nuclear glycerolipid metabolism in the central nervous system. We detected diacylglycerol lipase, monoacylglycerol lipase, and lysophosphate phosphatase enzymatic activities, which are responsible for maintaining the levels of endocannabinoid 2-arachidonoyl-glycerol (2-AG), a bioactive lipid mediator. The endocannabinoid system is a cellular signaling mechanism with a protective role in many pathophysiological processes, especially in the nervous system. 2-AG activates CB1 and CB2 GPCR receptors and triggers different signaling cascades, modulating intracellular Ca2+ levels, ERK1/2 phosphorylation, and other second messengers. Different studies have reported that CB1 is expressed not only in plasma membrane but also in intracellular compartments where they also seem to be functional. Therefore, the aim of this work was to study CB1 expression and function in isolated nuclei from rat cerebral cortex (CC). To this end, CC from Wistar rats were dissected, homogenized and highly purified nuclei (CCN) were isolated on a sucrose-density ultracentrifugation. CB1 protein expression was observed in CCN as well as in isolated cerebellum nuclei by Western Blot, which was confirmed by immunocytochemistry. In order to evaluate if CB1 is functional, CCN were incubated at 37 °C with a CB1 agonist (WIN 55-212-2) and ERK1/2 and Akt signaling cascades were studied by Western Blot. Interestingly, it was observed that ERK1/2 phosphorylation increased in nuclei treated with WIN 5 µM for 30 min with respect to controls (p<0.01) while no changes were seen in Akt phosphorylation. Taken together, these results demonstrate that CB1 has also a nuclear localization in the cerebral cortex where it could have a potential role in chromatin regulation and gene expression. |
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2017 |
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The cannabinoid receptor CB1 is expressed and functional in isolated nuclei of rat cerebral cortex; LXII Reunión Anual de la Sociedad Argentina de Investigación Clínica; LII Reunión Anual de la Sociedad Argentina de Investigación Bioquímica y Biología Molecular; LXV Reunión Anual de la Sociedad Argentina de Inmunología; Reunión de la Sociedad Argentina de Andrología; XLVI Reunión Anual de la Sociedad Argentina de Biofísica; XIX Reunión Anual de la Sociedad Argentina de Biología; XLIX Reunión Anual de la Sociedad Argentina de Farmacología Experimental; Reunión Anual de la Sociedad Argentina de Fisiología; Reunión de la Sociedad Argentina de Hematología y XXIX Reunión Anual de la Sociedad Argentina de Protozoología; Buenos Aires; Argentina; 2017; 307-308 0076-6046 1669-9106 CONICET Digital CONICET |
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