Nuclear lipid metabolism is differently regulated by polyunsaturated fatty acids during aging

Autores
Gaveglio, Virginia Lucía; Pascual, Ana Clara; Giusto, Norma Maria; Pasquaré, Susana Juana
Año de publicación
2015
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Former studies from our lab demonstrated an active nuclear lipid metabolism in central nervous system that is modified by aging. We detected several nuclear enzymatic activities related to glycerolipid metabolism, such as lipid phosphate phosphatase (LPP), diacylglycerol lipase (DAGL), monoacylglycerol lipase (MAGL), phospholipase A (PLA) and lysophosphate phosphatase (LPAPase). Interestingly, we also observed that they could be regulated by retinoic acid and polyunsaturated fatty acids (PUFA) through an unknown non-genomic mechanism in adult nuclei. Therefore, the aim of this work was to study the modulation of these enzymatic activities by arachidonic acid (AA) and docosahexaenoic acid (DHA) in nuclei from cerebellum of aged rats. To this end, rat cerebellums (28 mo) were homogenized and highly purified nuclei were isolated by sucrose-density ultracentrifugation. Using the respective radiolabelled substrates co-incubated with these PUFA, we observed that AA and DHA promote a major DAG availability by increasing and decreasing LPPs and DAGL activity, respectively. A minor MAG availability was also observed due to a diminution on PLA and LPAPase activities. These results demonstrate a different PUFA-regulated lipid metabolism in aged nuclei with respect to adults which could be involved in signaling events related to the epigenetic changes during aging.
Fil: Gaveglio, Virginia Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Pascual, Ana Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Pasquaré, Susana Juana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
LI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Mar del Plata
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
AGING
PUFA
LIPID METABOLISM
NUCLEI
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/237702
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/237702
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Nuclear lipid metabolism is differently regulated by polyunsaturated fatty acids during agingGaveglio, Virginia LucíaPascual, Ana ClaraGiusto, Norma MariaPasquaré, Susana JuanaAGINGPUFALIPID METABOLISMNUCLEIhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Former studies from our lab demonstrated an active nuclear lipid metabolism in central nervous system that is modified by aging. We detected several nuclear enzymatic activities related to glycerolipid metabolism, such as lipid phosphate phosphatase (LPP), diacylglycerol lipase (DAGL), monoacylglycerol lipase (MAGL), phospholipase A (PLA) and lysophosphate phosphatase (LPAPase). Interestingly, we also observed that they could be regulated by retinoic acid and polyunsaturated fatty acids (PUFA) through an unknown non-genomic mechanism in adult nuclei. Therefore, the aim of this work was to study the modulation of these enzymatic activities by arachidonic acid (AA) and docosahexaenoic acid (DHA) in nuclei from cerebellum of aged rats. To this end, rat cerebellums (28 mo) were homogenized and highly purified nuclei were isolated by sucrose-density ultracentrifugation. Using the respective radiolabelled substrates co-incubated with these PUFA, we observed that AA and DHA promote a major DAG availability by increasing and decreasing LPPs and DAGL activity, respectively. A minor MAG availability was also observed due to a diminution on PLA and LPAPase activities. These results demonstrate a different PUFA-regulated lipid metabolism in aged nuclei with respect to adults which could be involved in signaling events related to the epigenetic changes during aging.Fil: Gaveglio, Virginia Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Pascual, Ana Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaFil: Pasquaré, Susana Juana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; ArgentinaLI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología MolecularMar del PlataArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularTech Science Press2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/237702Nuclear lipid metabolism is differently regulated by polyunsaturated fatty acids during aging; LI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Mar del Plata; Argentina; 2015; 43-430327-95451667-5746CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://newsite.saib.org.ar/publicaciones/Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:45:33Zoai:ri.conicet.gov.ar:11336/237702instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:45:33.892CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nuclear lipid metabolism is differently regulated by polyunsaturated fatty acids during aging
title Nuclear lipid metabolism is differently regulated by polyunsaturated fatty acids during aging
spellingShingle Nuclear lipid metabolism is differently regulated by polyunsaturated fatty acids during aging
Gaveglio, Virginia Lucía
AGING
PUFA
LIPID METABOLISM
NUCLEI
title_short Nuclear lipid metabolism is differently regulated by polyunsaturated fatty acids during aging
title_full Nuclear lipid metabolism is differently regulated by polyunsaturated fatty acids during aging
title_fullStr Nuclear lipid metabolism is differently regulated by polyunsaturated fatty acids during aging
title_full_unstemmed Nuclear lipid metabolism is differently regulated by polyunsaturated fatty acids during aging
title_sort Nuclear lipid metabolism is differently regulated by polyunsaturated fatty acids during aging
dc.creator.none.fl_str_mv Gaveglio, Virginia Lucía
Pascual, Ana Clara
Giusto, Norma Maria
Pasquaré, Susana Juana
author Gaveglio, Virginia Lucía
author_facet Gaveglio, Virginia Lucía
Pascual, Ana Clara
Giusto, Norma Maria
Pasquaré, Susana Juana
author_role author
author2 Pascual, Ana Clara
Giusto, Norma Maria
Pasquaré, Susana Juana
author2_role author
author
author
dc.subject.none.fl_str_mv AGING
PUFA
LIPID METABOLISM
NUCLEI
topic AGING
PUFA
LIPID METABOLISM
NUCLEI
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Former studies from our lab demonstrated an active nuclear lipid metabolism in central nervous system that is modified by aging. We detected several nuclear enzymatic activities related to glycerolipid metabolism, such as lipid phosphate phosphatase (LPP), diacylglycerol lipase (DAGL), monoacylglycerol lipase (MAGL), phospholipase A (PLA) and lysophosphate phosphatase (LPAPase). Interestingly, we also observed that they could be regulated by retinoic acid and polyunsaturated fatty acids (PUFA) through an unknown non-genomic mechanism in adult nuclei. Therefore, the aim of this work was to study the modulation of these enzymatic activities by arachidonic acid (AA) and docosahexaenoic acid (DHA) in nuclei from cerebellum of aged rats. To this end, rat cerebellums (28 mo) were homogenized and highly purified nuclei were isolated by sucrose-density ultracentrifugation. Using the respective radiolabelled substrates co-incubated with these PUFA, we observed that AA and DHA promote a major DAG availability by increasing and decreasing LPPs and DAGL activity, respectively. A minor MAG availability was also observed due to a diminution on PLA and LPAPase activities. These results demonstrate a different PUFA-regulated lipid metabolism in aged nuclei with respect to adults which could be involved in signaling events related to the epigenetic changes during aging.
Fil: Gaveglio, Virginia Lucía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Pascual, Ana Clara. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Giusto, Norma Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
Fil: Pasquaré, Susana Juana. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Investigaciones Bioquímicas de Bahía Blanca. Universidad Nacional del Sur. Instituto de Investigaciones Bioquímicas de Bahía Blanca; Argentina
LI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Mar del Plata
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description Former studies from our lab demonstrated an active nuclear lipid metabolism in central nervous system that is modified by aging. We detected several nuclear enzymatic activities related to glycerolipid metabolism, such as lipid phosphate phosphatase (LPP), diacylglycerol lipase (DAGL), monoacylglycerol lipase (MAGL), phospholipase A (PLA) and lysophosphate phosphatase (LPAPase). Interestingly, we also observed that they could be regulated by retinoic acid and polyunsaturated fatty acids (PUFA) through an unknown non-genomic mechanism in adult nuclei. Therefore, the aim of this work was to study the modulation of these enzymatic activities by arachidonic acid (AA) and docosahexaenoic acid (DHA) in nuclei from cerebellum of aged rats. To this end, rat cerebellums (28 mo) were homogenized and highly purified nuclei were isolated by sucrose-density ultracentrifugation. Using the respective radiolabelled substrates co-incubated with these PUFA, we observed that AA and DHA promote a major DAG availability by increasing and decreasing LPPs and DAGL activity, respectively. A minor MAG availability was also observed due to a diminution on PLA and LPAPase activities. These results demonstrate a different PUFA-regulated lipid metabolism in aged nuclei with respect to adults which could be involved in signaling events related to the epigenetic changes during aging.
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/237702
Nuclear lipid metabolism is differently regulated by polyunsaturated fatty acids during aging; LI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Mar del Plata; Argentina; 2015; 43-43
0327-9545
1667-5746
CONICET Digital
CONICET
url http://hdl.handle.net/11336/237702
identifier_str_mv Nuclear lipid metabolism is differently regulated by polyunsaturated fatty acids during aging; LI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Mar del Plata; Argentina; 2015; 43-43
0327-9545
1667-5746
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://newsite.saib.org.ar/publicaciones/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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