Perinatal ethanol exposure affects cell populations in adult dorsal hippocampal neurogenic niche

Autores
Villalba, Nerina; Madarnas, Catalina; Bressano, Julieta; Sanchez, Viviana; Brusco, Herminia Alicia
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Neurodevelopment is highly affected by perinatal ethanol exposure (PEE). In the adult brain, neurogenesis takes place in the dentate gyrus (DG) of the hippocampus and in the subventricular zone. This work aimed to analyze the effect of PEE on the cellular types involved in adult dorsal hippocampal neurogenesis phases using a murine model. For this purpose, primiparous female CD1 mice consumed only ethanol 6% v/v from 20 days prior to mating and along pregnancy and lactation to ensure that the pups were exposed to ethanol throughout pre- and early postnatal development. After weaning, pups had no further contact with ethanol. Cell types of the adult male dorsal DG were studied by immunofluorescence. A lower percentage of type 1 cells and immature neurons and a higher percentage of type 2 cells were observed in PEE animals. This decrease in type 1 cells suggests that PEE reduces the population of remnant progenitors of the dorsal DG present in adulthood. The increase in type 2 cells and the decrease in immature neurons indicate that, during neurodevelopment, ethanol alters the capacity of neuroblasts to become neurons in the adult neurogenic niche. These results suggest that pathways implicated in cell determination are affected by PEE and remain affected in adulthood.
Fil: Villalba, Nerina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Madarnas, Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Bressano, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Sanchez, Viviana. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Brusco, Herminia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Materia
ADULT NEUROGENESIS
DENTATE GYRUS
ETHANOL
HIPPOCAMPUS
NEURONAL PROGENITOR
PERINATAL ETHANOL EXPOSURE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/227749
Acceder
id CONICETDig_c76f075ae36e0145983630ec9e3d9752
oai_identifier_str oai:ri.conicet.gov.ar:11336/227749
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Perinatal ethanol exposure affects cell populations in adult dorsal hippocampal neurogenic nicheVillalba, NerinaMadarnas, CatalinaBressano, JulietaSanchez, VivianaBrusco, Herminia AliciaADULT NEUROGENESISDENTATE GYRUSETHANOLHIPPOCAMPUSNEURONAL PROGENITORPERINATAL ETHANOL EXPOSUREhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Neurodevelopment is highly affected by perinatal ethanol exposure (PEE). In the adult brain, neurogenesis takes place in the dentate gyrus (DG) of the hippocampus and in the subventricular zone. This work aimed to analyze the effect of PEE on the cellular types involved in adult dorsal hippocampal neurogenesis phases using a murine model. For this purpose, primiparous female CD1 mice consumed only ethanol 6% v/v from 20 days prior to mating and along pregnancy and lactation to ensure that the pups were exposed to ethanol throughout pre- and early postnatal development. After weaning, pups had no further contact with ethanol. Cell types of the adult male dorsal DG were studied by immunofluorescence. A lower percentage of type 1 cells and immature neurons and a higher percentage of type 2 cells were observed in PEE animals. This decrease in type 1 cells suggests that PEE reduces the population of remnant progenitors of the dorsal DG present in adulthood. The increase in type 2 cells and the decrease in immature neurons indicate that, during neurodevelopment, ethanol alters the capacity of neuroblasts to become neurons in the adult neurogenic niche. These results suggest that pathways implicated in cell determination are affected by PEE and remain affected in adulthood.Fil: Villalba, Nerina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaFil: Madarnas, Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaFil: Bressano, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaFil: Sanchez, Viviana. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Brusco, Herminia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; ArgentinaElsevier Ireland2023-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/227749Villalba, Nerina; Madarnas, Catalina; Bressano, Julieta; Sanchez, Viviana; Brusco, Herminia Alicia; Perinatal ethanol exposure affects cell populations in adult dorsal hippocampal neurogenic niche; Elsevier Ireland; Neuroscience Research.; 198; 7-2023; 8-200168-0102CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0168010223001360info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neures.2023.07.001info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:52:14Zoai:ri.conicet.gov.ar:11336/227749instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:52:14.422CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Perinatal ethanol exposure affects cell populations in adult dorsal hippocampal neurogenic niche
title Perinatal ethanol exposure affects cell populations in adult dorsal hippocampal neurogenic niche
spellingShingle Perinatal ethanol exposure affects cell populations in adult dorsal hippocampal neurogenic niche
Villalba, Nerina
ADULT NEUROGENESIS
DENTATE GYRUS
ETHANOL
HIPPOCAMPUS
NEURONAL PROGENITOR
PERINATAL ETHANOL EXPOSURE
title_short Perinatal ethanol exposure affects cell populations in adult dorsal hippocampal neurogenic niche
title_full Perinatal ethanol exposure affects cell populations in adult dorsal hippocampal neurogenic niche
title_fullStr Perinatal ethanol exposure affects cell populations in adult dorsal hippocampal neurogenic niche
title_full_unstemmed Perinatal ethanol exposure affects cell populations in adult dorsal hippocampal neurogenic niche
title_sort Perinatal ethanol exposure affects cell populations in adult dorsal hippocampal neurogenic niche
dc.creator.none.fl_str_mv Villalba, Nerina
Madarnas, Catalina
Bressano, Julieta
Sanchez, Viviana
Brusco, Herminia Alicia
author Villalba, Nerina
author_facet Villalba, Nerina
Madarnas, Catalina
Bressano, Julieta
Sanchez, Viviana
Brusco, Herminia Alicia
author_role author
author2 Madarnas, Catalina
Bressano, Julieta
Sanchez, Viviana
Brusco, Herminia Alicia
author2_role author
author
author
author
dc.subject.none.fl_str_mv ADULT NEUROGENESIS
DENTATE GYRUS
ETHANOL
HIPPOCAMPUS
NEURONAL PROGENITOR
PERINATAL ETHANOL EXPOSURE
topic ADULT NEUROGENESIS
DENTATE GYRUS
ETHANOL
HIPPOCAMPUS
NEURONAL PROGENITOR
PERINATAL ETHANOL EXPOSURE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Neurodevelopment is highly affected by perinatal ethanol exposure (PEE). In the adult brain, neurogenesis takes place in the dentate gyrus (DG) of the hippocampus and in the subventricular zone. This work aimed to analyze the effect of PEE on the cellular types involved in adult dorsal hippocampal neurogenesis phases using a murine model. For this purpose, primiparous female CD1 mice consumed only ethanol 6% v/v from 20 days prior to mating and along pregnancy and lactation to ensure that the pups were exposed to ethanol throughout pre- and early postnatal development. After weaning, pups had no further contact with ethanol. Cell types of the adult male dorsal DG were studied by immunofluorescence. A lower percentage of type 1 cells and immature neurons and a higher percentage of type 2 cells were observed in PEE animals. This decrease in type 1 cells suggests that PEE reduces the population of remnant progenitors of the dorsal DG present in adulthood. The increase in type 2 cells and the decrease in immature neurons indicate that, during neurodevelopment, ethanol alters the capacity of neuroblasts to become neurons in the adult neurogenic niche. These results suggest that pathways implicated in cell determination are affected by PEE and remain affected in adulthood.
Fil: Villalba, Nerina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Madarnas, Catalina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Bressano, Julieta. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
Fil: Sanchez, Viviana. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Brusco, Herminia Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Biología Celular y Neurociencia "Prof. Eduardo de Robertis". Universidad de Buenos Aires. Facultad de Medicina. Instituto de Biología Celular y Neurociencia; Argentina
description Neurodevelopment is highly affected by perinatal ethanol exposure (PEE). In the adult brain, neurogenesis takes place in the dentate gyrus (DG) of the hippocampus and in the subventricular zone. This work aimed to analyze the effect of PEE on the cellular types involved in adult dorsal hippocampal neurogenesis phases using a murine model. For this purpose, primiparous female CD1 mice consumed only ethanol 6% v/v from 20 days prior to mating and along pregnancy and lactation to ensure that the pups were exposed to ethanol throughout pre- and early postnatal development. After weaning, pups had no further contact with ethanol. Cell types of the adult male dorsal DG were studied by immunofluorescence. A lower percentage of type 1 cells and immature neurons and a higher percentage of type 2 cells were observed in PEE animals. This decrease in type 1 cells suggests that PEE reduces the population of remnant progenitors of the dorsal DG present in adulthood. The increase in type 2 cells and the decrease in immature neurons indicate that, during neurodevelopment, ethanol alters the capacity of neuroblasts to become neurons in the adult neurogenic niche. These results suggest that pathways implicated in cell determination are affected by PEE and remain affected in adulthood.
publishDate 2023
dc.date.none.fl_str_mv 2023-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/227749
Villalba, Nerina; Madarnas, Catalina; Bressano, Julieta; Sanchez, Viviana; Brusco, Herminia Alicia; Perinatal ethanol exposure affects cell populations in adult dorsal hippocampal neurogenic niche; Elsevier Ireland; Neuroscience Research.; 198; 7-2023; 8-20
0168-0102
CONICET Digital
CONICET
url http://hdl.handle.net/11336/227749
identifier_str_mv Villalba, Nerina; Madarnas, Catalina; Bressano, Julieta; Sanchez, Viviana; Brusco, Herminia Alicia; Perinatal ethanol exposure affects cell populations in adult dorsal hippocampal neurogenic niche; Elsevier Ireland; Neuroscience Research.; 198; 7-2023; 8-20
0168-0102
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0168010223001360
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.neures.2023.07.001
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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