Bifidobacteria upregulate expression of toll-like receptor negative regulators counteracting enterotoxigenic Escherichia coli mediated inflammation in bovine intestinal epitheliocy...

Autores
Murata, Kozue; Villena, Julio Cesar; Tomosada, Yohsuke; Risa, Hara; Chiba, Eriko; Shimazu, Tomoyuki; Aso, Hisashi; Suda, Yoshihito; Iwabuchi, Noriyuki; Xiao, Jin-zhong; Saito, Tadao; Kitazawa, Haruki
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We previously established a bovine intestinal epithelial cell line (BIE cells) and showed that BIE cells are useful in vitro model system for the study of interactions between pathogenic and beneficial microorganisms and bovine intestinal epithelial cells (IECs). In the present study we aimed to select potential immunomodulatory bifidobacteria that may be used to beneficially modulate the inflammatory response in bovine IECs. We also aimed to gain insight in the molecular mechanisms involved in the anti-inflammatory effect of bifidobacteria by evaluating the role of Toll-like receptor (TLR)-2 and TLR negative regulators in the regulation of proinflamatory cytokines production and MAPK, NF-κB and PI3K pathways activation in BIE cells. Five bifidobacteria strains were evaluated in this study and according to their capacity to modulate inflammatory response of BIE cells. Despite the unique effect of each strain, four common points were found when comparing the effect of the high and moderate anti-inflammatory strains: 1) Upregulation of TLR negative regulators and the intensity of that upregulation was related to the different immunomodulatory capacity of each bifidobacteria strain. 2) The balance between MAPK activation and MKP-1 upregulation affected the an- ti-inflammatory effect of bifidobacteria in BIE cells. 3) The inhibition of PI3K pathway was related to the an- ti-inflammatory effect of bifidobacteria. 4) The immunoregulatory effect of bifidobacteria in BIE cells is partially de- pendent on TLR2. This study shows that BIE cells can be used for the selection of immunoregulatory bifidobacteria and for studying the mechanisms involved in the protective activity of immunobiotics against TLR4-induced inflammatory damage. In addition, we have demonstrated that the anti-inflammatory effect of bifidobacteria was achieved by a com- plex interaction of multiple TLRs negative regulators as well as the inhibition/activation of multiple signaling pathways.
Fil: Murata, Kozue. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Tomosada, Yohsuke. Tohoku University; Japón
Fil: Risa, Hara. Tohoku University; Japón
Fil: Chiba, Eriko. Tohoku University; Japón
Fil: Shimazu, Tomoyuki. Tohoku University; Japón
Fil: Aso, Hisashi. Tohoku University; Japón
Fil: Suda, Yoshihito. Miyagi University; Japón
Fil: Iwabuchi, Noriyuki. Miyagi University; Japón
Fil: Xiao, Jin-zhong. Miyagi University; Japón
Fil: Saito, Tadao. Tohoku University; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón
Materia
Toll-Like Receptor Negative Regulators
Bifidobacteria
Bovine Intestinal Epitheliocytes
Enterotoxigenic Escherichia Coli
Bovine Intestinal Epithelial Cells
Tlrs Negative Regulators
Etec
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/2392
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/2392
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Bifidobacteria upregulate expression of toll-like receptor negative regulators counteracting enterotoxigenic Escherichia coli mediated inflammation in bovine intestinal epitheliocytesMurata, KozueVillena, Julio CesarTomosada, YohsukeRisa, HaraChiba, ErikoShimazu, TomoyukiAso, HisashiSuda, YoshihitoIwabuchi, NoriyukiXiao, Jin-zhongSaito, TadaoKitazawa, HarukiToll-Like Receptor Negative RegulatorsBifidobacteriaBovine Intestinal EpitheliocytesEnterotoxigenic Escherichia ColiBovine Intestinal Epithelial CellsTlrs Negative RegulatorsEtechttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3We previously established a bovine intestinal epithelial cell line (BIE cells) and showed that BIE cells are useful in vitro model system for the study of interactions between pathogenic and beneficial microorganisms and bovine intestinal epithelial cells (IECs). In the present study we aimed to select potential immunomodulatory bifidobacteria that may be used to beneficially modulate the inflammatory response in bovine IECs. We also aimed to gain insight in the molecular mechanisms involved in the anti-inflammatory effect of bifidobacteria by evaluating the role of Toll-like receptor (TLR)-2 and TLR negative regulators in the regulation of proinflamatory cytokines production and MAPK, NF-κB and PI3K pathways activation in BIE cells. Five bifidobacteria strains were evaluated in this study and according to their capacity to modulate inflammatory response of BIE cells. Despite the unique effect of each strain, four common points were found when comparing the effect of the high and moderate anti-inflammatory strains: 1) Upregulation of TLR negative regulators and the intensity of that upregulation was related to the different immunomodulatory capacity of each bifidobacteria strain. 2) The balance between MAPK activation and MKP-1 upregulation affected the an- ti-inflammatory effect of bifidobacteria in BIE cells. 3) The inhibition of PI3K pathway was related to the an- ti-inflammatory effect of bifidobacteria. 4) The immunoregulatory effect of bifidobacteria in BIE cells is partially de- pendent on TLR2. This study shows that BIE cells can be used for the selection of immunoregulatory bifidobacteria and for studying the mechanisms involved in the protective activity of immunobiotics against TLR4-induced inflammatory damage. In addition, we have demonstrated that the anti-inflammatory effect of bifidobacteria was achieved by a com- plex interaction of multiple TLRs negative regulators as well as the inhibition/activation of multiple signaling pathways.Fil: Murata, Kozue. Tohoku University; JapónFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); ArgentinaFil: Tomosada, Yohsuke. Tohoku University; JapónFil: Risa, Hara. Tohoku University; JapónFil: Chiba, Eriko. Tohoku University; JapónFil: Shimazu, Tomoyuki. Tohoku University; JapónFil: Aso, Hisashi. Tohoku University; JapónFil: Suda, Yoshihito. Miyagi University; JapónFil: Iwabuchi, Noriyuki. Miyagi University; JapónFil: Xiao, Jin-zhong. Miyagi University; JapónFil: Saito, Tadao. Tohoku University; JapónFil: Kitazawa, Haruki. Tohoku University; JapónScientific Research2013-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/2392Murata, Kozue; Villena, Julio Cesar; Tomosada, Yohsuke; Risa, Hara; Chiba, Eriko; et al.; Bifidobacteria upregulate expression of toll-like receptor negative regulators counteracting enterotoxigenic Escherichia coli mediated inflammation in bovine intestinal epitheliocytes; Scientific Research; Open Journal of Veterinary Medicine; 3; 2; 6-2013; 143-1552165-33562165-3364enginfo:eu-repo/semantics/altIdentifier/url/http://www.scirp.org/Journal/PaperInformation.aspx?PaperID=32697info:eu-repo/semantics/altIdentifier/doi/10.4236/ojvm.2013.32023info:eu-repo/semantics/altIdentifier/url/http://www.scirp.org/journal/ojvminfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:07:47Zoai:ri.conicet.gov.ar:11336/2392instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:07:47.414CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Bifidobacteria upregulate expression of toll-like receptor negative regulators counteracting enterotoxigenic Escherichia coli mediated inflammation in bovine intestinal epitheliocytes
title Bifidobacteria upregulate expression of toll-like receptor negative regulators counteracting enterotoxigenic Escherichia coli mediated inflammation in bovine intestinal epitheliocytes
spellingShingle Bifidobacteria upregulate expression of toll-like receptor negative regulators counteracting enterotoxigenic Escherichia coli mediated inflammation in bovine intestinal epitheliocytes
Murata, Kozue
Toll-Like Receptor Negative Regulators
Bifidobacteria
Bovine Intestinal Epitheliocytes
Enterotoxigenic Escherichia Coli
Bovine Intestinal Epithelial Cells
Tlrs Negative Regulators
Etec
title_short Bifidobacteria upregulate expression of toll-like receptor negative regulators counteracting enterotoxigenic Escherichia coli mediated inflammation in bovine intestinal epitheliocytes
title_full Bifidobacteria upregulate expression of toll-like receptor negative regulators counteracting enterotoxigenic Escherichia coli mediated inflammation in bovine intestinal epitheliocytes
title_fullStr Bifidobacteria upregulate expression of toll-like receptor negative regulators counteracting enterotoxigenic Escherichia coli mediated inflammation in bovine intestinal epitheliocytes
title_full_unstemmed Bifidobacteria upregulate expression of toll-like receptor negative regulators counteracting enterotoxigenic Escherichia coli mediated inflammation in bovine intestinal epitheliocytes
title_sort Bifidobacteria upregulate expression of toll-like receptor negative regulators counteracting enterotoxigenic Escherichia coli mediated inflammation in bovine intestinal epitheliocytes
dc.creator.none.fl_str_mv Murata, Kozue
Villena, Julio Cesar
Tomosada, Yohsuke
Risa, Hara
Chiba, Eriko
Shimazu, Tomoyuki
Aso, Hisashi
Suda, Yoshihito
Iwabuchi, Noriyuki
Xiao, Jin-zhong
Saito, Tadao
Kitazawa, Haruki
author Murata, Kozue
author_facet Murata, Kozue
Villena, Julio Cesar
Tomosada, Yohsuke
Risa, Hara
Chiba, Eriko
Shimazu, Tomoyuki
Aso, Hisashi
Suda, Yoshihito
Iwabuchi, Noriyuki
Xiao, Jin-zhong
Saito, Tadao
Kitazawa, Haruki
author_role author
author2 Villena, Julio Cesar
Tomosada, Yohsuke
Risa, Hara
Chiba, Eriko
Shimazu, Tomoyuki
Aso, Hisashi
Suda, Yoshihito
Iwabuchi, Noriyuki
Xiao, Jin-zhong
Saito, Tadao
Kitazawa, Haruki
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Toll-Like Receptor Negative Regulators
Bifidobacteria
Bovine Intestinal Epitheliocytes
Enterotoxigenic Escherichia Coli
Bovine Intestinal Epithelial Cells
Tlrs Negative Regulators
Etec
topic Toll-Like Receptor Negative Regulators
Bifidobacteria
Bovine Intestinal Epitheliocytes
Enterotoxigenic Escherichia Coli
Bovine Intestinal Epithelial Cells
Tlrs Negative Regulators
Etec
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv We previously established a bovine intestinal epithelial cell line (BIE cells) and showed that BIE cells are useful in vitro model system for the study of interactions between pathogenic and beneficial microorganisms and bovine intestinal epithelial cells (IECs). In the present study we aimed to select potential immunomodulatory bifidobacteria that may be used to beneficially modulate the inflammatory response in bovine IECs. We also aimed to gain insight in the molecular mechanisms involved in the anti-inflammatory effect of bifidobacteria by evaluating the role of Toll-like receptor (TLR)-2 and TLR negative regulators in the regulation of proinflamatory cytokines production and MAPK, NF-κB and PI3K pathways activation in BIE cells. Five bifidobacteria strains were evaluated in this study and according to their capacity to modulate inflammatory response of BIE cells. Despite the unique effect of each strain, four common points were found when comparing the effect of the high and moderate anti-inflammatory strains: 1) Upregulation of TLR negative regulators and the intensity of that upregulation was related to the different immunomodulatory capacity of each bifidobacteria strain. 2) The balance between MAPK activation and MKP-1 upregulation affected the an- ti-inflammatory effect of bifidobacteria in BIE cells. 3) The inhibition of PI3K pathway was related to the an- ti-inflammatory effect of bifidobacteria. 4) The immunoregulatory effect of bifidobacteria in BIE cells is partially de- pendent on TLR2. This study shows that BIE cells can be used for the selection of immunoregulatory bifidobacteria and for studying the mechanisms involved in the protective activity of immunobiotics against TLR4-induced inflammatory damage. In addition, we have demonstrated that the anti-inflammatory effect of bifidobacteria was achieved by a com- plex interaction of multiple TLRs negative regulators as well as the inhibition/activation of multiple signaling pathways.
Fil: Murata, Kozue. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tucumán. Centro de Referencia para Lactobacilos (i); Argentina
Fil: Tomosada, Yohsuke. Tohoku University; Japón
Fil: Risa, Hara. Tohoku University; Japón
Fil: Chiba, Eriko. Tohoku University; Japón
Fil: Shimazu, Tomoyuki. Tohoku University; Japón
Fil: Aso, Hisashi. Tohoku University; Japón
Fil: Suda, Yoshihito. Miyagi University; Japón
Fil: Iwabuchi, Noriyuki. Miyagi University; Japón
Fil: Xiao, Jin-zhong. Miyagi University; Japón
Fil: Saito, Tadao. Tohoku University; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón
description We previously established a bovine intestinal epithelial cell line (BIE cells) and showed that BIE cells are useful in vitro model system for the study of interactions between pathogenic and beneficial microorganisms and bovine intestinal epithelial cells (IECs). In the present study we aimed to select potential immunomodulatory bifidobacteria that may be used to beneficially modulate the inflammatory response in bovine IECs. We also aimed to gain insight in the molecular mechanisms involved in the anti-inflammatory effect of bifidobacteria by evaluating the role of Toll-like receptor (TLR)-2 and TLR negative regulators in the regulation of proinflamatory cytokines production and MAPK, NF-κB and PI3K pathways activation in BIE cells. Five bifidobacteria strains were evaluated in this study and according to their capacity to modulate inflammatory response of BIE cells. Despite the unique effect of each strain, four common points were found when comparing the effect of the high and moderate anti-inflammatory strains: 1) Upregulation of TLR negative regulators and the intensity of that upregulation was related to the different immunomodulatory capacity of each bifidobacteria strain. 2) The balance between MAPK activation and MKP-1 upregulation affected the an- ti-inflammatory effect of bifidobacteria in BIE cells. 3) The inhibition of PI3K pathway was related to the an- ti-inflammatory effect of bifidobacteria. 4) The immunoregulatory effect of bifidobacteria in BIE cells is partially de- pendent on TLR2. This study shows that BIE cells can be used for the selection of immunoregulatory bifidobacteria and for studying the mechanisms involved in the protective activity of immunobiotics against TLR4-induced inflammatory damage. In addition, we have demonstrated that the anti-inflammatory effect of bifidobacteria was achieved by a com- plex interaction of multiple TLRs negative regulators as well as the inhibition/activation of multiple signaling pathways.
publishDate 2013
dc.date.none.fl_str_mv 2013-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/2392
Murata, Kozue; Villena, Julio Cesar; Tomosada, Yohsuke; Risa, Hara; Chiba, Eriko; et al.; Bifidobacteria upregulate expression of toll-like receptor negative regulators counteracting enterotoxigenic Escherichia coli mediated inflammation in bovine intestinal epitheliocytes; Scientific Research; Open Journal of Veterinary Medicine; 3; 2; 6-2013; 143-155
2165-3356
2165-3364
url http://hdl.handle.net/11336/2392
identifier_str_mv Murata, Kozue; Villena, Julio Cesar; Tomosada, Yohsuke; Risa, Hara; Chiba, Eriko; et al.; Bifidobacteria upregulate expression of toll-like receptor negative regulators counteracting enterotoxigenic Escherichia coli mediated inflammation in bovine intestinal epitheliocytes; Scientific Research; Open Journal of Veterinary Medicine; 3; 2; 6-2013; 143-155
2165-3356
2165-3364
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.scirp.org/Journal/PaperInformation.aspx?PaperID=32697
info:eu-repo/semantics/altIdentifier/doi/10.4236/ojvm.2013.32023
info:eu-repo/semantics/altIdentifier/url/http://www.scirp.org/journal/ojvm
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Scientific Research
publisher.none.fl_str_mv Scientific Research
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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