Modulation of porcine intestinal epitheliocytes immunetranscriptome response by Lactobacillus jensenii TL2937

Autores
Kobayashi, Hisakazu; Albarracín, Leonardo Miguel; Sato, Nana; Kanmani, Paulraj; Kober, Humayun A.K.M.; Ikeda-Ohtsubo, Wakako; Suda, Yoshihito; Nochi, Tomonori; Aso, Hisashi; Makino, Seiya; Kano, Hiroshi; Ohkawara, Sou; Saito, Tadao; Villena, Julio Cesar; Kitazawa, Haruki
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In order to evaluate probiotic strains applicable for the beneficial immunomodulation of the porcine gut (immunobiotics), we previously developed a porcine intestinal epitheliocyte cell line (PIE cells). Here, transcriptomic studies using PIE cells were performed considering that this information would be valuable for understanding the mechanisms involved in the protective activity of the immunobiotic strain Lactobacillus jensenii TL2937 against intestinal inflammatory damage in pigs. In addition, those studies would provide criteria for selecting biomarkers for the screening of new immunobiotic strains. We performed microarray analysis to investigate the transcriptomic response of PIE cells to the challenge with heat-stable Enterotoxigenic Escherichia coli (ETEC) pathogen-associated molecular patterns (PAMPs) and, the changes induced by L. jensenii TL2937 in that response. The approach allowed us to obtain a global overview of the immune genes involved in the response of PIE cells to heat-stable ETEC PAMPs. We observed that L. jensenii TL2937 differently modulated gene expression in ETEC PAMPs-challenged PIE cells. Microarray and RT-PCR analysis indicated that the most remarkable changes in PIE cells transcriptomic profile after heat-stable ETEC PAMPs challenge were observed in chemokines, adhesion molecules, complement and coagulation cascades factors. In addition, an anti-inflammatory effect triggered by TL2937 strain in PIE cells was clearly demonstrated. The decrease in the expression of chemokines (CCL8, CXCL5, CXCL9, CXCL10, and CXCL11), complement (C1R, C1S, C3, and CFB), and coagulation factors (F3) by L. jensenii TL2937 supports our previous reports on the immunoregulatory effect of this strain. These results provided clues for the better understanding of the mechanism underlying host-immunobiotic interaction in the porcine host. The comprehensive transcriptomic profiles of PIE cells provided by our analyses successfully identified a group of genes, which could be used as prospective biomarkers for the screening and evaluation of new anti-inflammatory immunobiotics for the prevention of inflammatory intestinal disorders in pigs.
Fil: Kobayashi, Hisakazu. Tohoku University; Japón
Fil: Albarracín, Leonardo Miguel. Tohoku University; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Sato, Nana. Tohoku University; Japón
Fil: Kanmani, Paulraj. Tohoku University; Japón
Fil: Kober, Humayun A.K.M.. Tohoku University; Japón. Chittagong Veterinary and Animal Sciences University. Department of Dairy and Poultry Science; Bangladesh
Fil: Ikeda-Ohtsubo, Wakako. Tohoku University; Japón
Fil: Suda, Yoshihito. Miyagi University; Japón
Fil: Nochi, Tomonori. Tohoku University; Japón
Fil: Aso, Hisashi. Tohoku University; Japón
Fil: Makino, Seiya. Meiji Co., Ltd. Food Science Research Labs.; Japón
Fil: Kano, Hiroshi. Meiji Co., Ltd. Food Science Research Labs.; Japón
Fil: Ohkawara, Sou. Meiji Seika Pharma Co., Ltd. Agricultural and Veterinary Division; Japón
Fil: Saito, Tadao. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón
Materia
PORCINE INTESTINAL EPITHELIOCYTES
IMMUNOTRANSCRIPTOMIC RESPONSE
ETEC
LACTOBACILLUS JENSENII TL2937
IMMUNOBIOTIC BIOMARKERS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/80200
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/80200
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Modulation of porcine intestinal epitheliocytes immunetranscriptome response by Lactobacillus jensenii TL2937Kobayashi, HisakazuAlbarracín, Leonardo MiguelSato, NanaKanmani, PaulrajKober, Humayun A.K.M.Ikeda-Ohtsubo, WakakoSuda, YoshihitoNochi, TomonoriAso, HisashiMakino, SeiyaKano, HiroshiOhkawara, SouSaito, TadaoVillena, Julio CesarKitazawa, HarukiPORCINE INTESTINAL EPITHELIOCYTESIMMUNOTRANSCRIPTOMIC RESPONSEETECLACTOBACILLUS JENSENII TL2937IMMUNOBIOTIC BIOMARKERShttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3In order to evaluate probiotic strains applicable for the beneficial immunomodulation of the porcine gut (immunobiotics), we previously developed a porcine intestinal epitheliocyte cell line (PIE cells). Here, transcriptomic studies using PIE cells were performed considering that this information would be valuable for understanding the mechanisms involved in the protective activity of the immunobiotic strain Lactobacillus jensenii TL2937 against intestinal inflammatory damage in pigs. In addition, those studies would provide criteria for selecting biomarkers for the screening of new immunobiotic strains. We performed microarray analysis to investigate the transcriptomic response of PIE cells to the challenge with heat-stable Enterotoxigenic Escherichia coli (ETEC) pathogen-associated molecular patterns (PAMPs) and, the changes induced by L. jensenii TL2937 in that response. The approach allowed us to obtain a global overview of the immune genes involved in the response of PIE cells to heat-stable ETEC PAMPs. We observed that L. jensenii TL2937 differently modulated gene expression in ETEC PAMPs-challenged PIE cells. Microarray and RT-PCR analysis indicated that the most remarkable changes in PIE cells transcriptomic profile after heat-stable ETEC PAMPs challenge were observed in chemokines, adhesion molecules, complement and coagulation cascades factors. In addition, an anti-inflammatory effect triggered by TL2937 strain in PIE cells was clearly demonstrated. The decrease in the expression of chemokines (CCL8, CXCL5, CXCL9, CXCL10, and CXCL11), complement (C1R, C1S, C3, and CFB), and coagulation factors (F3) by L. jensenii TL2937 supports our previous reports on the immunoregulatory effect of this strain. These results provided clues for the better understanding of the mechanism underlying host-immunobiotic interaction in the porcine host. The comprehensive transcriptomic profiles of PIE cells provided by our analyses successfully identified a group of genes, which could be used as prospective biomarkers for the screening and evaluation of new anti-inflammatory immunobiotics for the prevention of inflammatory intestinal disorders in pigs.Fil: Kobayashi, Hisakazu. Tohoku University; JapónFil: Albarracín, Leonardo Miguel. Tohoku University; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Sato, Nana. Tohoku University; JapónFil: Kanmani, Paulraj. Tohoku University; JapónFil: Kober, Humayun A.K.M.. Tohoku University; Japón. Chittagong Veterinary and Animal Sciences University. Department of Dairy and Poultry Science; BangladeshFil: Ikeda-Ohtsubo, Wakako. Tohoku University; JapónFil: Suda, Yoshihito. Miyagi University; JapónFil: Nochi, Tomonori. Tohoku University; JapónFil: Aso, Hisashi. Tohoku University; JapónFil: Makino, Seiya. Meiji Co., Ltd. Food Science Research Labs.; JapónFil: Kano, Hiroshi. Meiji Co., Ltd. Food Science Research Labs.; JapónFil: Ohkawara, Sou. Meiji Seika Pharma Co., Ltd. Agricultural and Veterinary Division; JapónFil: Saito, Tadao. Tohoku University; JapónFil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; JapónFil: Kitazawa, Haruki. Tohoku University; JapónWageningen Academic Publishers2016-11-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/80200Kobayashi, Hisakazu; Albarracín, Leonardo Miguel; Sato, Nana; Kanmani, Paulraj; Kober, Humayun A.K.M.; et al.; Modulation of porcine intestinal epitheliocytes immunetranscriptome response by Lactobacillus jensenii TL2937; Wageningen Academic Publishers; Beneficial microbes; 7; 5; 30-11-2016; 769-7821876-28831876-2891CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3920/BM2016.0095info:eu-repo/semantics/altIdentifier/url/https://www.wageningenacademic.com/doi/10.3920/BM2016.0095info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:36:17Zoai:ri.conicet.gov.ar:11336/80200instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:36:17.471CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Modulation of porcine intestinal epitheliocytes immunetranscriptome response by Lactobacillus jensenii TL2937
title Modulation of porcine intestinal epitheliocytes immunetranscriptome response by Lactobacillus jensenii TL2937
spellingShingle Modulation of porcine intestinal epitheliocytes immunetranscriptome response by Lactobacillus jensenii TL2937
Kobayashi, Hisakazu
PORCINE INTESTINAL EPITHELIOCYTES
IMMUNOTRANSCRIPTOMIC RESPONSE
ETEC
LACTOBACILLUS JENSENII TL2937
IMMUNOBIOTIC BIOMARKERS
title_short Modulation of porcine intestinal epitheliocytes immunetranscriptome response by Lactobacillus jensenii TL2937
title_full Modulation of porcine intestinal epitheliocytes immunetranscriptome response by Lactobacillus jensenii TL2937
title_fullStr Modulation of porcine intestinal epitheliocytes immunetranscriptome response by Lactobacillus jensenii TL2937
title_full_unstemmed Modulation of porcine intestinal epitheliocytes immunetranscriptome response by Lactobacillus jensenii TL2937
title_sort Modulation of porcine intestinal epitheliocytes immunetranscriptome response by Lactobacillus jensenii TL2937
dc.creator.none.fl_str_mv Kobayashi, Hisakazu
Albarracín, Leonardo Miguel
Sato, Nana
Kanmani, Paulraj
Kober, Humayun A.K.M.
Ikeda-Ohtsubo, Wakako
Suda, Yoshihito
Nochi, Tomonori
Aso, Hisashi
Makino, Seiya
Kano, Hiroshi
Ohkawara, Sou
Saito, Tadao
Villena, Julio Cesar
Kitazawa, Haruki
author Kobayashi, Hisakazu
author_facet Kobayashi, Hisakazu
Albarracín, Leonardo Miguel
Sato, Nana
Kanmani, Paulraj
Kober, Humayun A.K.M.
Ikeda-Ohtsubo, Wakako
Suda, Yoshihito
Nochi, Tomonori
Aso, Hisashi
Makino, Seiya
Kano, Hiroshi
Ohkawara, Sou
Saito, Tadao
Villena, Julio Cesar
Kitazawa, Haruki
author_role author
author2 Albarracín, Leonardo Miguel
Sato, Nana
Kanmani, Paulraj
Kober, Humayun A.K.M.
Ikeda-Ohtsubo, Wakako
Suda, Yoshihito
Nochi, Tomonori
Aso, Hisashi
Makino, Seiya
Kano, Hiroshi
Ohkawara, Sou
Saito, Tadao
Villena, Julio Cesar
Kitazawa, Haruki
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv PORCINE INTESTINAL EPITHELIOCYTES
IMMUNOTRANSCRIPTOMIC RESPONSE
ETEC
LACTOBACILLUS JENSENII TL2937
IMMUNOBIOTIC BIOMARKERS
topic PORCINE INTESTINAL EPITHELIOCYTES
IMMUNOTRANSCRIPTOMIC RESPONSE
ETEC
LACTOBACILLUS JENSENII TL2937
IMMUNOBIOTIC BIOMARKERS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv In order to evaluate probiotic strains applicable for the beneficial immunomodulation of the porcine gut (immunobiotics), we previously developed a porcine intestinal epitheliocyte cell line (PIE cells). Here, transcriptomic studies using PIE cells were performed considering that this information would be valuable for understanding the mechanisms involved in the protective activity of the immunobiotic strain Lactobacillus jensenii TL2937 against intestinal inflammatory damage in pigs. In addition, those studies would provide criteria for selecting biomarkers for the screening of new immunobiotic strains. We performed microarray analysis to investigate the transcriptomic response of PIE cells to the challenge with heat-stable Enterotoxigenic Escherichia coli (ETEC) pathogen-associated molecular patterns (PAMPs) and, the changes induced by L. jensenii TL2937 in that response. The approach allowed us to obtain a global overview of the immune genes involved in the response of PIE cells to heat-stable ETEC PAMPs. We observed that L. jensenii TL2937 differently modulated gene expression in ETEC PAMPs-challenged PIE cells. Microarray and RT-PCR analysis indicated that the most remarkable changes in PIE cells transcriptomic profile after heat-stable ETEC PAMPs challenge were observed in chemokines, adhesion molecules, complement and coagulation cascades factors. In addition, an anti-inflammatory effect triggered by TL2937 strain in PIE cells was clearly demonstrated. The decrease in the expression of chemokines (CCL8, CXCL5, CXCL9, CXCL10, and CXCL11), complement (C1R, C1S, C3, and CFB), and coagulation factors (F3) by L. jensenii TL2937 supports our previous reports on the immunoregulatory effect of this strain. These results provided clues for the better understanding of the mechanism underlying host-immunobiotic interaction in the porcine host. The comprehensive transcriptomic profiles of PIE cells provided by our analyses successfully identified a group of genes, which could be used as prospective biomarkers for the screening and evaluation of new anti-inflammatory immunobiotics for the prevention of inflammatory intestinal disorders in pigs.
Fil: Kobayashi, Hisakazu. Tohoku University; Japón
Fil: Albarracín, Leonardo Miguel. Tohoku University; Japón. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Sato, Nana. Tohoku University; Japón
Fil: Kanmani, Paulraj. Tohoku University; Japón
Fil: Kober, Humayun A.K.M.. Tohoku University; Japón. Chittagong Veterinary and Animal Sciences University. Department of Dairy and Poultry Science; Bangladesh
Fil: Ikeda-Ohtsubo, Wakako. Tohoku University; Japón
Fil: Suda, Yoshihito. Miyagi University; Japón
Fil: Nochi, Tomonori. Tohoku University; Japón
Fil: Aso, Hisashi. Tohoku University; Japón
Fil: Makino, Seiya. Meiji Co., Ltd. Food Science Research Labs.; Japón
Fil: Kano, Hiroshi. Meiji Co., Ltd. Food Science Research Labs.; Japón
Fil: Ohkawara, Sou. Meiji Seika Pharma Co., Ltd. Agricultural and Veterinary Division; Japón
Fil: Saito, Tadao. Tohoku University; Japón
Fil: Villena, Julio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Tohoku University; Japón
Fil: Kitazawa, Haruki. Tohoku University; Japón
description In order to evaluate probiotic strains applicable for the beneficial immunomodulation of the porcine gut (immunobiotics), we previously developed a porcine intestinal epitheliocyte cell line (PIE cells). Here, transcriptomic studies using PIE cells were performed considering that this information would be valuable for understanding the mechanisms involved in the protective activity of the immunobiotic strain Lactobacillus jensenii TL2937 against intestinal inflammatory damage in pigs. In addition, those studies would provide criteria for selecting biomarkers for the screening of new immunobiotic strains. We performed microarray analysis to investigate the transcriptomic response of PIE cells to the challenge with heat-stable Enterotoxigenic Escherichia coli (ETEC) pathogen-associated molecular patterns (PAMPs) and, the changes induced by L. jensenii TL2937 in that response. The approach allowed us to obtain a global overview of the immune genes involved in the response of PIE cells to heat-stable ETEC PAMPs. We observed that L. jensenii TL2937 differently modulated gene expression in ETEC PAMPs-challenged PIE cells. Microarray and RT-PCR analysis indicated that the most remarkable changes in PIE cells transcriptomic profile after heat-stable ETEC PAMPs challenge were observed in chemokines, adhesion molecules, complement and coagulation cascades factors. In addition, an anti-inflammatory effect triggered by TL2937 strain in PIE cells was clearly demonstrated. The decrease in the expression of chemokines (CCL8, CXCL5, CXCL9, CXCL10, and CXCL11), complement (C1R, C1S, C3, and CFB), and coagulation factors (F3) by L. jensenii TL2937 supports our previous reports on the immunoregulatory effect of this strain. These results provided clues for the better understanding of the mechanism underlying host-immunobiotic interaction in the porcine host. The comprehensive transcriptomic profiles of PIE cells provided by our analyses successfully identified a group of genes, which could be used as prospective biomarkers for the screening and evaluation of new anti-inflammatory immunobiotics for the prevention of inflammatory intestinal disorders in pigs.
publishDate 2016
dc.date.none.fl_str_mv 2016-11-30
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/80200
Kobayashi, Hisakazu; Albarracín, Leonardo Miguel; Sato, Nana; Kanmani, Paulraj; Kober, Humayun A.K.M.; et al.; Modulation of porcine intestinal epitheliocytes immunetranscriptome response by Lactobacillus jensenii TL2937; Wageningen Academic Publishers; Beneficial microbes; 7; 5; 30-11-2016; 769-782
1876-2883
1876-2891
CONICET Digital
CONICET
url http://hdl.handle.net/11336/80200
identifier_str_mv Kobayashi, Hisakazu; Albarracín, Leonardo Miguel; Sato, Nana; Kanmani, Paulraj; Kober, Humayun A.K.M.; et al.; Modulation of porcine intestinal epitheliocytes immunetranscriptome response by Lactobacillus jensenii TL2937; Wageningen Academic Publishers; Beneficial microbes; 7; 5; 30-11-2016; 769-782
1876-2883
1876-2891
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3920/BM2016.0095
info:eu-repo/semantics/altIdentifier/url/https://www.wageningenacademic.com/doi/10.3920/BM2016.0095
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wageningen Academic Publishers
publisher.none.fl_str_mv Wageningen Academic Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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