Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina

Autores
Craig, María Isabel; Rojas, María Florencia; Van der Ploeg, Claudia A.; Olivera, Valeria; Vagnozzi, Ariel Eduardo; Perez, Andres Maximiliano; König, Guido Alberto
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Avian infectious laryngotracheitis (ILT) is a worldwide infectious disease that causes important economic losses in the poultry industry. Although it is known that ILT virus (ILTV) is present in Argentina, there is no information about the circulating strains. With the aim to characterize them, seven different genomic regions (thymidine kinase, glycoproteins D, G, B, C, and J, and infected cell polypeptide 4) were partially sequenced and compared between field samples. The gJ sequence resulted to be the most informative segment, it allowed the differentiation among field sample strains, and also, between wild and vaccine viruses. Specific changes in selected nucleotidic positions led to the definition of five distinct haplotypes. Tests for detection of clustering were run to test the null hypothesis that ILTV haplotypes were randomly distributed in time in Argentina and in space in the most densely populated poultry region of this country, Entre Rios. From this study, it was possible to identify a 46 km radius cluster in which higher proportions of haplotypes 4 and 5 were observed, next to a provincial route in Entre Rios and a significant decline of haplotype 5 between 2009 and 2011. Results here provide an update on the molecular epidemiology of ILT in Argentina, including data on specific genome segments that may be used for rapid characterization of the virus in the field. Ultimately, results will contribute to the surveillance of ILT in the country.
Fil: Craig, María Isabel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Rojas, María Florencia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios. Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio de Sanidad Aviar; Argentina
Fil: Van der Ploeg, Claudia A.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Olivera, Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Vagnozzi, Ariel Eduardo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Perez, Andres Maximiliano. University of Minnesota; Estados Unidos
Fil: König, Guido Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Materia
ARGENTINA
EPIDEMIOLOGY
GLYCOPROTEIN J
INFECTIOUS LARYNGOTRACHEITIS VIRUS
MOLECULAR CHARACTERIZATION
SPATIAL CLUSTER ANALYSIS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/41662
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/41662
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from ArgentinaCraig, María IsabelRojas, María FlorenciaVan der Ploeg, Claudia A.Olivera, ValeriaVagnozzi, Ariel EduardoPerez, Andres MaximilianoKönig, Guido AlbertoARGENTINAEPIDEMIOLOGYGLYCOPROTEIN JINFECTIOUS LARYNGOTRACHEITIS VIRUSMOLECULAR CHARACTERIZATIONSPATIAL CLUSTER ANALYSIShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Avian infectious laryngotracheitis (ILT) is a worldwide infectious disease that causes important economic losses in the poultry industry. Although it is known that ILT virus (ILTV) is present in Argentina, there is no information about the circulating strains. With the aim to characterize them, seven different genomic regions (thymidine kinase, glycoproteins D, G, B, C, and J, and infected cell polypeptide 4) were partially sequenced and compared between field samples. The gJ sequence resulted to be the most informative segment, it allowed the differentiation among field sample strains, and also, between wild and vaccine viruses. Specific changes in selected nucleotidic positions led to the definition of five distinct haplotypes. Tests for detection of clustering were run to test the null hypothesis that ILTV haplotypes were randomly distributed in time in Argentina and in space in the most densely populated poultry region of this country, Entre Rios. From this study, it was possible to identify a 46 km radius cluster in which higher proportions of haplotypes 4 and 5 were observed, next to a provincial route in Entre Rios and a significant decline of haplotype 5 between 2009 and 2011. Results here provide an update on the molecular epidemiology of ILT in Argentina, including data on specific genome segments that may be used for rapid characterization of the virus in the field. Ultimately, results will contribute to the surveillance of ILT in the country.Fil: Craig, María Isabel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Rojas, María Florencia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios. Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio de Sanidad Aviar; ArgentinaFil: Van der Ploeg, Claudia A.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; ArgentinaFil: Olivera, Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Vagnozzi, Ariel Eduardo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; ArgentinaFil: Perez, Andres Maximiliano. University of Minnesota; Estados UnidosFil: König, Guido Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFrontiers Media S.A.2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/41662Craig, María Isabel; Rojas, María Florencia; Van der Ploeg, Claudia A.; Olivera, Valeria; Vagnozzi, Ariel Eduardo; et al.; Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina; Frontiers Media S.A.; Frontiers in Veterinary Science; 4; DEC; 12-2017; 1-72297-1769CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fvets.2017.00212info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fvets.2017.00212/fullinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:51:54Zoai:ri.conicet.gov.ar:11336/41662instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:51:54.779CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina
title Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina
spellingShingle Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina
Craig, María Isabel
ARGENTINA
EPIDEMIOLOGY
GLYCOPROTEIN J
INFECTIOUS LARYNGOTRACHEITIS VIRUS
MOLECULAR CHARACTERIZATION
SPATIAL CLUSTER ANALYSIS
title_short Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina
title_full Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina
title_fullStr Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina
title_full_unstemmed Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina
title_sort Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina
dc.creator.none.fl_str_mv Craig, María Isabel
Rojas, María Florencia
Van der Ploeg, Claudia A.
Olivera, Valeria
Vagnozzi, Ariel Eduardo
Perez, Andres Maximiliano
König, Guido Alberto
author Craig, María Isabel
author_facet Craig, María Isabel
Rojas, María Florencia
Van der Ploeg, Claudia A.
Olivera, Valeria
Vagnozzi, Ariel Eduardo
Perez, Andres Maximiliano
König, Guido Alberto
author_role author
author2 Rojas, María Florencia
Van der Ploeg, Claudia A.
Olivera, Valeria
Vagnozzi, Ariel Eduardo
Perez, Andres Maximiliano
König, Guido Alberto
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ARGENTINA
EPIDEMIOLOGY
GLYCOPROTEIN J
INFECTIOUS LARYNGOTRACHEITIS VIRUS
MOLECULAR CHARACTERIZATION
SPATIAL CLUSTER ANALYSIS
topic ARGENTINA
EPIDEMIOLOGY
GLYCOPROTEIN J
INFECTIOUS LARYNGOTRACHEITIS VIRUS
MOLECULAR CHARACTERIZATION
SPATIAL CLUSTER ANALYSIS
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Avian infectious laryngotracheitis (ILT) is a worldwide infectious disease that causes important economic losses in the poultry industry. Although it is known that ILT virus (ILTV) is present in Argentina, there is no information about the circulating strains. With the aim to characterize them, seven different genomic regions (thymidine kinase, glycoproteins D, G, B, C, and J, and infected cell polypeptide 4) were partially sequenced and compared between field samples. The gJ sequence resulted to be the most informative segment, it allowed the differentiation among field sample strains, and also, between wild and vaccine viruses. Specific changes in selected nucleotidic positions led to the definition of five distinct haplotypes. Tests for detection of clustering were run to test the null hypothesis that ILTV haplotypes were randomly distributed in time in Argentina and in space in the most densely populated poultry region of this country, Entre Rios. From this study, it was possible to identify a 46 km radius cluster in which higher proportions of haplotypes 4 and 5 were observed, next to a provincial route in Entre Rios and a significant decline of haplotype 5 between 2009 and 2011. Results here provide an update on the molecular epidemiology of ILT in Argentina, including data on specific genome segments that may be used for rapid characterization of the virus in the field. Ultimately, results will contribute to the surveillance of ILT in the country.
Fil: Craig, María Isabel. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Rojas, María Florencia. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Entre Rios. Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio de Sanidad Aviar; Argentina
Fil: Van der Ploeg, Claudia A.. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Olivera, Valeria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Vagnozzi, Ariel Eduardo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Virología; Argentina
Fil: Perez, Andres Maximiliano. University of Minnesota; Estados Unidos
Fil: König, Guido Alberto. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
description Avian infectious laryngotracheitis (ILT) is a worldwide infectious disease that causes important economic losses in the poultry industry. Although it is known that ILT virus (ILTV) is present in Argentina, there is no information about the circulating strains. With the aim to characterize them, seven different genomic regions (thymidine kinase, glycoproteins D, G, B, C, and J, and infected cell polypeptide 4) were partially sequenced and compared between field samples. The gJ sequence resulted to be the most informative segment, it allowed the differentiation among field sample strains, and also, between wild and vaccine viruses. Specific changes in selected nucleotidic positions led to the definition of five distinct haplotypes. Tests for detection of clustering were run to test the null hypothesis that ILTV haplotypes were randomly distributed in time in Argentina and in space in the most densely populated poultry region of this country, Entre Rios. From this study, it was possible to identify a 46 km radius cluster in which higher proportions of haplotypes 4 and 5 were observed, next to a provincial route in Entre Rios and a significant decline of haplotype 5 between 2009 and 2011. Results here provide an update on the molecular epidemiology of ILT in Argentina, including data on specific genome segments that may be used for rapid characterization of the virus in the field. Ultimately, results will contribute to the surveillance of ILT in the country.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/41662
Craig, María Isabel; Rojas, María Florencia; Van der Ploeg, Claudia A.; Olivera, Valeria; Vagnozzi, Ariel Eduardo; et al.; Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina; Frontiers Media S.A.; Frontiers in Veterinary Science; 4; DEC; 12-2017; 1-7
2297-1769
CONICET Digital
CONICET
url http://hdl.handle.net/11336/41662
identifier_str_mv Craig, María Isabel; Rojas, María Florencia; Van der Ploeg, Claudia A.; Olivera, Valeria; Vagnozzi, Ariel Eduardo; et al.; Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina; Frontiers Media S.A.; Frontiers in Veterinary Science; 4; DEC; 12-2017; 1-7
2297-1769
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fvets.2017.00212
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/articles/10.3389/fvets.2017.00212/full
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media S.A.
publisher.none.fl_str_mv Frontiers Media S.A.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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