Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage
- Autores
- Vera, Federico Sebastian; Craig, María Isabel; Olivera, Valeria Soledad; Rojas, María Florencia; Konig, Guido Alberto; Pereda, Ariel Julian; Vagnozzi, Ariel Eduardo
- Año de publicación
- 2015
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- In Argentina, classical vaccines are used to control infectious bursal disease virus (IBDV); however, outbreaks of IBDV are frequently observed. This could be due to failures in the vaccination programs or to the emergence of new strains, which would be able to break through the protection given by vaccines. Hence, genetic characterization of the viruses responsible for the outbreaks that occurred in recent years is crucial for the evaluation of the control programs and the understanding of the epidemiology and evolution of IBDV. In this study, we characterized 51 field samples collected in Argentina (previously identified as IBDV positive) through the analysis of previously identified apomorphic sequences. Phylogenetic analysis of regVP2 showed that 42 samples formed a unique cluster (Argentinean lineage), seven samples were typical classical strains (one of them was a vaccine strain), and two belonged to the very virulent lineage (vvIBDV). Interestingly, when the analysis was performed on the regVP1 sequences, the field samples segregated similarly to regVP2; thus, we observed no evidence of a reassortment event in the Argentinean samples. Amino acid sequence analysis of regVP2 showed a particular pattern of residues in the Argentinean lineage, particularly the presence of T272, P289 and F296, which had not been reported before as signature sequences for any IBDV phenotype. Notably, the residue S254, characteristic of the antigenic variant, was not present in any of the Argentinean samples.
EEA Concepción del Uruguay
Fil: Vera, Federico Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio Sanidad Aviar; Argentina
Fil: Craig, María Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Olivera, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Rojas, María Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio Sanidad Aviar; Argentina
Fil: Konig, Guido Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Pereda, Ariel Julian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Vagnozzi, Ariel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio Sanidad Aviar; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina - Fuente
- Archives of Virology 160 (8) : 1909–1921 (August 2015)
- Materia
-
Virus Bursitis Infecciosa
Genética
Vacuna
Antígenos
Epidemiología
Infectious Bursal Disease Virus
Genetics
Vaccines
Antigens
Epidemiology
Argentina - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/2968
Ver los metadatos del registro completo
id |
INTADig_d3efdc47887240b02b104f927458809a |
---|---|
oai_identifier_str |
oai:localhost:20.500.12123/2968 |
network_acronym_str |
INTADig |
repository_id_str |
l |
network_name_str |
INTA Digital (INTA) |
spelling |
Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineageVera, Federico SebastianCraig, María IsabelOlivera, Valeria SoledadRojas, María FlorenciaKonig, Guido AlbertoPereda, Ariel JulianVagnozzi, Ariel EduardoVirus Bursitis InfecciosaGenéticaVacunaAntígenosEpidemiologíaInfectious Bursal Disease VirusGeneticsVaccinesAntigensEpidemiologyArgentinaIn Argentina, classical vaccines are used to control infectious bursal disease virus (IBDV); however, outbreaks of IBDV are frequently observed. This could be due to failures in the vaccination programs or to the emergence of new strains, which would be able to break through the protection given by vaccines. Hence, genetic characterization of the viruses responsible for the outbreaks that occurred in recent years is crucial for the evaluation of the control programs and the understanding of the epidemiology and evolution of IBDV. In this study, we characterized 51 field samples collected in Argentina (previously identified as IBDV positive) through the analysis of previously identified apomorphic sequences. Phylogenetic analysis of regVP2 showed that 42 samples formed a unique cluster (Argentinean lineage), seven samples were typical classical strains (one of them was a vaccine strain), and two belonged to the very virulent lineage (vvIBDV). Interestingly, when the analysis was performed on the regVP1 sequences, the field samples segregated similarly to regVP2; thus, we observed no evidence of a reassortment event in the Argentinean samples. Amino acid sequence analysis of regVP2 showed a particular pattern of residues in the Argentinean lineage, particularly the presence of T272, P289 and F296, which had not been reported before as signature sequences for any IBDV phenotype. Notably, the residue S254, characteristic of the antigenic variant, was not present in any of the Argentinean samples.EEA Concepción del UruguayFil: Vera, Federico Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio Sanidad Aviar; ArgentinaFil: Craig, María Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Olivera, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Rojas, María Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio Sanidad Aviar; ArgentinaFil: Konig, Guido Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Pereda, Ariel Julian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Vagnozzi, Ariel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio Sanidad Aviar; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina2018-08-03T13:13:11Z2018-08-03T13:13:11Z2015-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://link.springer.com/article/10.1007/s00705-015-2449-4http://hdl.handle.net/20.500.12123/29680304-86081432-8798https://doi.org/10.1007/s00705-015-2449-4Archives of Virology 160 (8) : 1909–1921 (August 2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:23Zoai:localhost:20.500.12123/2968instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:24.082INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage |
title |
Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage |
spellingShingle |
Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage Vera, Federico Sebastian Virus Bursitis Infecciosa Genética Vacuna Antígenos Epidemiología Infectious Bursal Disease Virus Genetics Vaccines Antigens Epidemiology Argentina |
title_short |
Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage |
title_full |
Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage |
title_fullStr |
Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage |
title_full_unstemmed |
Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage |
title_sort |
Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage |
dc.creator.none.fl_str_mv |
Vera, Federico Sebastian Craig, María Isabel Olivera, Valeria Soledad Rojas, María Florencia Konig, Guido Alberto Pereda, Ariel Julian Vagnozzi, Ariel Eduardo |
author |
Vera, Federico Sebastian |
author_facet |
Vera, Federico Sebastian Craig, María Isabel Olivera, Valeria Soledad Rojas, María Florencia Konig, Guido Alberto Pereda, Ariel Julian Vagnozzi, Ariel Eduardo |
author_role |
author |
author2 |
Craig, María Isabel Olivera, Valeria Soledad Rojas, María Florencia Konig, Guido Alberto Pereda, Ariel Julian Vagnozzi, Ariel Eduardo |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Virus Bursitis Infecciosa Genética Vacuna Antígenos Epidemiología Infectious Bursal Disease Virus Genetics Vaccines Antigens Epidemiology Argentina |
topic |
Virus Bursitis Infecciosa Genética Vacuna Antígenos Epidemiología Infectious Bursal Disease Virus Genetics Vaccines Antigens Epidemiology Argentina |
dc.description.none.fl_txt_mv |
In Argentina, classical vaccines are used to control infectious bursal disease virus (IBDV); however, outbreaks of IBDV are frequently observed. This could be due to failures in the vaccination programs or to the emergence of new strains, which would be able to break through the protection given by vaccines. Hence, genetic characterization of the viruses responsible for the outbreaks that occurred in recent years is crucial for the evaluation of the control programs and the understanding of the epidemiology and evolution of IBDV. In this study, we characterized 51 field samples collected in Argentina (previously identified as IBDV positive) through the analysis of previously identified apomorphic sequences. Phylogenetic analysis of regVP2 showed that 42 samples formed a unique cluster (Argentinean lineage), seven samples were typical classical strains (one of them was a vaccine strain), and two belonged to the very virulent lineage (vvIBDV). Interestingly, when the analysis was performed on the regVP1 sequences, the field samples segregated similarly to regVP2; thus, we observed no evidence of a reassortment event in the Argentinean samples. Amino acid sequence analysis of regVP2 showed a particular pattern of residues in the Argentinean lineage, particularly the presence of T272, P289 and F296, which had not been reported before as signature sequences for any IBDV phenotype. Notably, the residue S254, characteristic of the antigenic variant, was not present in any of the Argentinean samples. EEA Concepción del Uruguay Fil: Vera, Federico Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio Sanidad Aviar; Argentina Fil: Craig, María Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Olivera, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Rojas, María Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio Sanidad Aviar; Argentina Fil: Konig, Guido Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Pereda, Ariel Julian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Vagnozzi, Ariel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio Sanidad Aviar; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina |
description |
In Argentina, classical vaccines are used to control infectious bursal disease virus (IBDV); however, outbreaks of IBDV are frequently observed. This could be due to failures in the vaccination programs or to the emergence of new strains, which would be able to break through the protection given by vaccines. Hence, genetic characterization of the viruses responsible for the outbreaks that occurred in recent years is crucial for the evaluation of the control programs and the understanding of the epidemiology and evolution of IBDV. In this study, we characterized 51 field samples collected in Argentina (previously identified as IBDV positive) through the analysis of previously identified apomorphic sequences. Phylogenetic analysis of regVP2 showed that 42 samples formed a unique cluster (Argentinean lineage), seven samples were typical classical strains (one of them was a vaccine strain), and two belonged to the very virulent lineage (vvIBDV). Interestingly, when the analysis was performed on the regVP1 sequences, the field samples segregated similarly to regVP2; thus, we observed no evidence of a reassortment event in the Argentinean samples. Amino acid sequence analysis of regVP2 showed a particular pattern of residues in the Argentinean lineage, particularly the presence of T272, P289 and F296, which had not been reported before as signature sequences for any IBDV phenotype. Notably, the residue S254, characteristic of the antigenic variant, was not present in any of the Argentinean samples. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-08 2018-08-03T13:13:11Z 2018-08-03T13:13:11Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://link.springer.com/article/10.1007/s00705-015-2449-4 http://hdl.handle.net/20.500.12123/2968 0304-8608 1432-8798 https://doi.org/10.1007/s00705-015-2449-4 |
url |
https://link.springer.com/article/10.1007/s00705-015-2449-4 http://hdl.handle.net/20.500.12123/2968 https://doi.org/10.1007/s00705-015-2449-4 |
identifier_str_mv |
0304-8608 1432-8798 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/restrictedAccess |
eu_rights_str_mv |
restrictedAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Archives of Virology 160 (8) : 1909–1921 (August 2015) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
reponame_str |
INTA Digital (INTA) |
collection |
INTA Digital (INTA) |
instname_str |
Instituto Nacional de Tecnología Agropecuaria |
repository.name.fl_str_mv |
INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
repository.mail.fl_str_mv |
tripaldi.nicolas@inta.gob.ar |
_version_ |
1842341357308346368 |
score |
12.623145 |