Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage

Autores
Vera, Federico Sebastian; Craig, María Isabel; Olivera, Valeria Soledad; Rojas, María Florencia; Konig, Guido Alberto; Pereda, Ariel Julian; Vagnozzi, Ariel Eduardo
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In Argentina, classical vaccines are used to control infectious bursal disease virus (IBDV); however, outbreaks of IBDV are frequently observed. This could be due to failures in the vaccination programs or to the emergence of new strains, which would be able to break through the protection given by vaccines. Hence, genetic characterization of the viruses responsible for the outbreaks that occurred in recent years is crucial for the evaluation of the control programs and the understanding of the epidemiology and evolution of IBDV. In this study, we characterized 51 field samples collected in Argentina (previously identified as IBDV positive) through the analysis of previously identified apomorphic sequences. Phylogenetic analysis of regVP2 showed that 42 samples formed a unique cluster (Argentinean lineage), seven samples were typical classical strains (one of them was a vaccine strain), and two belonged to the very virulent lineage (vvIBDV). Interestingly, when the analysis was performed on the regVP1 sequences, the field samples segregated similarly to regVP2; thus, we observed no evidence of a reassortment event in the Argentinean samples. Amino acid sequence analysis of regVP2 showed a particular pattern of residues in the Argentinean lineage, particularly the presence of T272, P289 and F296, which had not been reported before as signature sequences for any IBDV phenotype. Notably, the residue S254, characteristic of the antigenic variant, was not present in any of the Argentinean samples.
EEA Concepción del Uruguay
Fil: Vera, Federico Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio Sanidad Aviar; Argentina
Fil: Craig, Marí­a Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Olivera, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Rojas, María Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio Sanidad Aviar; Argentina
Fil: Konig, Guido Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Pereda, Ariel Julian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Vagnozzi, Ariel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio Sanidad Aviar; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fuente
Archives of Virology 160 (8) : 1909–1921 (August 2015)
Materia
Virus Bursitis Infecciosa
Genética
Vacuna
Antígenos
Epidemiología
Infectious Bursal Disease Virus
Genetics
Vaccines
Antigens
Epidemiology
Argentina
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/2968
Acceder
id INTADig_d3efdc47887240b02b104f927458809a
oai_identifier_str oai:localhost:20.500.12123/2968
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineageVera, Federico SebastianCraig, María IsabelOlivera, Valeria SoledadRojas, María FlorenciaKonig, Guido AlbertoPereda, Ariel JulianVagnozzi, Ariel EduardoVirus Bursitis InfecciosaGenéticaVacunaAntígenosEpidemiologíaInfectious Bursal Disease VirusGeneticsVaccinesAntigensEpidemiologyArgentinaIn Argentina, classical vaccines are used to control infectious bursal disease virus (IBDV); however, outbreaks of IBDV are frequently observed. This could be due to failures in the vaccination programs or to the emergence of new strains, which would be able to break through the protection given by vaccines. Hence, genetic characterization of the viruses responsible for the outbreaks that occurred in recent years is crucial for the evaluation of the control programs and the understanding of the epidemiology and evolution of IBDV. In this study, we characterized 51 field samples collected in Argentina (previously identified as IBDV positive) through the analysis of previously identified apomorphic sequences. Phylogenetic analysis of regVP2 showed that 42 samples formed a unique cluster (Argentinean lineage), seven samples were typical classical strains (one of them was a vaccine strain), and two belonged to the very virulent lineage (vvIBDV). Interestingly, when the analysis was performed on the regVP1 sequences, the field samples segregated similarly to regVP2; thus, we observed no evidence of a reassortment event in the Argentinean samples. Amino acid sequence analysis of regVP2 showed a particular pattern of residues in the Argentinean lineage, particularly the presence of T272, P289 and F296, which had not been reported before as signature sequences for any IBDV phenotype. Notably, the residue S254, characteristic of the antigenic variant, was not present in any of the Argentinean samples.EEA Concepción del UruguayFil: Vera, Federico Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio Sanidad Aviar; ArgentinaFil: Craig, Marí­a Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Olivera, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Rojas, María Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio Sanidad Aviar; ArgentinaFil: Konig, Guido Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Pereda, Ariel Julian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Vagnozzi, Ariel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio Sanidad Aviar; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina2018-08-03T13:13:11Z2018-08-03T13:13:11Z2015-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://link.springer.com/article/10.1007/s00705-015-2449-4http://hdl.handle.net/20.500.12123/29680304-86081432-8798https://doi.org/10.1007/s00705-015-2449-4Archives of Virology 160 (8) : 1909–1921 (August 2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:23Zoai:localhost:20.500.12123/2968instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:23.736INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage
title Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage
spellingShingle Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage
Vera, Federico Sebastian
Virus Bursitis Infecciosa
Genética
Vacuna
Antígenos
Epidemiología
Infectious Bursal Disease Virus
Genetics
Vaccines
Antigens
Epidemiology
Argentina
title_short Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage
title_full Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage
title_fullStr Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage
title_full_unstemmed Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage
title_sort Molecular characterization of infectious bursal disease virus (IBDV) isolated in Argentina indicates a regional lineage
dc.creator.none.fl_str_mv Vera, Federico Sebastian
Craig, María Isabel
Olivera, Valeria Soledad
Rojas, María Florencia
Konig, Guido Alberto
Pereda, Ariel Julian
Vagnozzi, Ariel Eduardo
author Vera, Federico Sebastian
author_facet Vera, Federico Sebastian
Craig, María Isabel
Olivera, Valeria Soledad
Rojas, María Florencia
Konig, Guido Alberto
Pereda, Ariel Julian
Vagnozzi, Ariel Eduardo
author_role author
author2 Craig, María Isabel
Olivera, Valeria Soledad
Rojas, María Florencia
Konig, Guido Alberto
Pereda, Ariel Julian
Vagnozzi, Ariel Eduardo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Virus Bursitis Infecciosa
Genética
Vacuna
Antígenos
Epidemiología
Infectious Bursal Disease Virus
Genetics
Vaccines
Antigens
Epidemiology
Argentina
topic Virus Bursitis Infecciosa
Genética
Vacuna
Antígenos
Epidemiología
Infectious Bursal Disease Virus
Genetics
Vaccines
Antigens
Epidemiology
Argentina
dc.description.none.fl_txt_mv In Argentina, classical vaccines are used to control infectious bursal disease virus (IBDV); however, outbreaks of IBDV are frequently observed. This could be due to failures in the vaccination programs or to the emergence of new strains, which would be able to break through the protection given by vaccines. Hence, genetic characterization of the viruses responsible for the outbreaks that occurred in recent years is crucial for the evaluation of the control programs and the understanding of the epidemiology and evolution of IBDV. In this study, we characterized 51 field samples collected in Argentina (previously identified as IBDV positive) through the analysis of previously identified apomorphic sequences. Phylogenetic analysis of regVP2 showed that 42 samples formed a unique cluster (Argentinean lineage), seven samples were typical classical strains (one of them was a vaccine strain), and two belonged to the very virulent lineage (vvIBDV). Interestingly, when the analysis was performed on the regVP1 sequences, the field samples segregated similarly to regVP2; thus, we observed no evidence of a reassortment event in the Argentinean samples. Amino acid sequence analysis of regVP2 showed a particular pattern of residues in the Argentinean lineage, particularly the presence of T272, P289 and F296, which had not been reported before as signature sequences for any IBDV phenotype. Notably, the residue S254, characteristic of the antigenic variant, was not present in any of the Argentinean samples.
EEA Concepción del Uruguay
Fil: Vera, Federico Sebastian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio Sanidad Aviar; Argentina
Fil: Craig, Marí­a Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Olivera, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Rojas, María Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio Sanidad Aviar; Argentina
Fil: Konig, Guido Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Pereda, Ariel Julian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Vagnozzi, Ariel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio Sanidad Aviar; Argentina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
description In Argentina, classical vaccines are used to control infectious bursal disease virus (IBDV); however, outbreaks of IBDV are frequently observed. This could be due to failures in the vaccination programs or to the emergence of new strains, which would be able to break through the protection given by vaccines. Hence, genetic characterization of the viruses responsible for the outbreaks that occurred in recent years is crucial for the evaluation of the control programs and the understanding of the epidemiology and evolution of IBDV. In this study, we characterized 51 field samples collected in Argentina (previously identified as IBDV positive) through the analysis of previously identified apomorphic sequences. Phylogenetic analysis of regVP2 showed that 42 samples formed a unique cluster (Argentinean lineage), seven samples were typical classical strains (one of them was a vaccine strain), and two belonged to the very virulent lineage (vvIBDV). Interestingly, when the analysis was performed on the regVP1 sequences, the field samples segregated similarly to regVP2; thus, we observed no evidence of a reassortment event in the Argentinean samples. Amino acid sequence analysis of regVP2 showed a particular pattern of residues in the Argentinean lineage, particularly the presence of T272, P289 and F296, which had not been reported before as signature sequences for any IBDV phenotype. Notably, the residue S254, characteristic of the antigenic variant, was not present in any of the Argentinean samples.
publishDate 2015
dc.date.none.fl_str_mv 2015-08
2018-08-03T13:13:11Z
2018-08-03T13:13:11Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://link.springer.com/article/10.1007/s00705-015-2449-4
http://hdl.handle.net/20.500.12123/2968
0304-8608
1432-8798
https://doi.org/10.1007/s00705-015-2449-4
url https://link.springer.com/article/10.1007/s00705-015-2449-4
http://hdl.handle.net/20.500.12123/2968
https://doi.org/10.1007/s00705-015-2449-4
identifier_str_mv 0304-8608
1432-8798
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Archives of Virology 160 (8) : 1909–1921 (August 2015)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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