Evaluation of the transcriptional status of host cytokines and viral genes in the trachea of vaccinated and non-vaccinated chickens after challenge with the infectious laryngotrach...

Autores
Vagnozzi, Ariel Eduardo; Riblet, Sylva; Zavala, Guillermo; Ecco, Roselene; Afonso, Claudio L.; García, Maricarmen
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Infectious laryngotracheitis is a highly contagious disease of chickens responsible for significant economic losses for the poultry industry worldwide. The disease is caused by Gallid herpesvirus-1 (GaHV-1) commonly known as the infectious laryngotracheitis virus. Although characterized by their potential to regain virulence, chicken embryo origin (CEO) vaccines are the most effective vaccines against laryngotracheitis as they significantly reduce the replication of challenge virus in the trachea and conjunctiva. Knowledge on the nature of protective immunity elicited by CEO vaccines is very limited. Therefore, elucidating the origin of the immune responses elicited by CEO vaccination is relevant for development of safer control strategies. In this study the transcription levels of key host immune genes (IFN-γ, IFN-β, IL-1β, IL-6, IL-8, IL-18) and viral genes (ICP4, ICP27, UL46, UL49), as well as viral genome loads in trachea were quantified at 6 and 12 hours post-challenge of CEO vaccinated and non-vaccinated chickens. Immediately after challenge a significant increase in IFN-γ gene expression was followed by a significant reduction in viral replication. In contrast to the rapid induction of IFN-γ, expression of the pro-inflammatory cytokines (IL-1β, IL-6, IL-8) and type I IFN β was either slightly reduced or remained at basal levels. These suggest that the former cytokines may not play important roles during immediate early responses induced by ILTV challenge in either vaccinated or non-vaccinated chickens. Overall, these results suggest that the rapid expression of IFN-γ may induce pathways of antiviral responses necessary for blocking early virus replication.
Instituto de Virología
Fil: Vagnozzi, Ariel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Riblet, Sylva. University of Georgia. College of Veterinary Medicine. Department of Population Health. Poultry Diagnostic and Research Center; Estados Unidos
Fil: Zavala, Guillermo. Avian Health International. Flowery Branch; Estados Unidos
Fil: Ecco, Roselene. Universidade Federal de Minas Gerais. Escola de Veterinária. Laboratorio de Patología; Brasil
Fil: Afonso, Claudio L. Southeast Poultry Research Laboratory; Estados Unidos
Fil: García, Maricarmen. University of Georgia. College of Veterinary Medicine. Department of Population Health. Poultry Diagnostic and Research Center; Estados Unidos
Fuente
Avian pathology 45 (1) : 106–113. (2016)
Materia
Laryngotracheitis
Cytokines
Immune Response
Interferons
Chickens
Animal Viruses
Laringotraqueitis
Citoquinas
Respuesta Inmunológica
Interferonas
Pollo
Virus de los Animales
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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spelling Evaluation of the transcriptional status of host cytokines and viral genes in the trachea of vaccinated and non-vaccinated chickens after challenge with the infectious laryngotracheitis virusVagnozzi, Ariel EduardoRiblet, SylvaZavala, GuillermoEcco, RoseleneAfonso, Claudio L.García, MaricarmenLaryngotracheitisCytokinesImmune ResponseInterferonsChickensAnimal VirusesLaringotraqueitisCitoquinasRespuesta InmunológicaInterferonasPolloVirus de los AnimalesInfectious laryngotracheitis is a highly contagious disease of chickens responsible for significant economic losses for the poultry industry worldwide. The disease is caused by Gallid herpesvirus-1 (GaHV-1) commonly known as the infectious laryngotracheitis virus. Although characterized by their potential to regain virulence, chicken embryo origin (CEO) vaccines are the most effective vaccines against laryngotracheitis as they significantly reduce the replication of challenge virus in the trachea and conjunctiva. Knowledge on the nature of protective immunity elicited by CEO vaccines is very limited. Therefore, elucidating the origin of the immune responses elicited by CEO vaccination is relevant for development of safer control strategies. In this study the transcription levels of key host immune genes (IFN-γ, IFN-β, IL-1β, IL-6, IL-8, IL-18) and viral genes (ICP4, ICP27, UL46, UL49), as well as viral genome loads in trachea were quantified at 6 and 12 hours post-challenge of CEO vaccinated and non-vaccinated chickens. Immediately after challenge a significant increase in IFN-γ gene expression was followed by a significant reduction in viral replication. In contrast to the rapid induction of IFN-γ, expression of the pro-inflammatory cytokines (IL-1β, IL-6, IL-8) and type I IFN β was either slightly reduced or remained at basal levels. These suggest that the former cytokines may not play important roles during immediate early responses induced by ILTV challenge in either vaccinated or non-vaccinated chickens. Overall, these results suggest that the rapid expression of IFN-γ may induce pathways of antiviral responses necessary for blocking early virus replication.Instituto de VirologíaFil: Vagnozzi, Ariel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Riblet, Sylva. University of Georgia. College of Veterinary Medicine. Department of Population Health. Poultry Diagnostic and Research Center; Estados UnidosFil: Zavala, Guillermo. Avian Health International. Flowery Branch; Estados UnidosFil: Ecco, Roselene. Universidade Federal de Minas Gerais. Escola de Veterinária. Laboratorio de Patología; BrasilFil: Afonso, Claudio L. Southeast Poultry Research Laboratory; Estados UnidosFil: García, Maricarmen. University of Georgia. College of Veterinary Medicine. Department of Population Health. Poultry Diagnostic and Research Center; Estados UnidosTaylor & Francis2019-02-13T18:22:15Z2019-02-13T18:22:15Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/44380307-94571465-3338 (Online)http://dx.doi.org/10.1080/03079457.2015.1126804Avian pathology 45 (1) : 106–113. (2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:34Zoai:localhost:20.500.12123/4438instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:34.961INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Evaluation of the transcriptional status of host cytokines and viral genes in the trachea of vaccinated and non-vaccinated chickens after challenge with the infectious laryngotracheitis virus
title Evaluation of the transcriptional status of host cytokines and viral genes in the trachea of vaccinated and non-vaccinated chickens after challenge with the infectious laryngotracheitis virus
spellingShingle Evaluation of the transcriptional status of host cytokines and viral genes in the trachea of vaccinated and non-vaccinated chickens after challenge with the infectious laryngotracheitis virus
Vagnozzi, Ariel Eduardo
Laryngotracheitis
Cytokines
Immune Response
Interferons
Chickens
Animal Viruses
Laringotraqueitis
Citoquinas
Respuesta Inmunológica
Interferonas
Pollo
Virus de los Animales
title_short Evaluation of the transcriptional status of host cytokines and viral genes in the trachea of vaccinated and non-vaccinated chickens after challenge with the infectious laryngotracheitis virus
title_full Evaluation of the transcriptional status of host cytokines and viral genes in the trachea of vaccinated and non-vaccinated chickens after challenge with the infectious laryngotracheitis virus
title_fullStr Evaluation of the transcriptional status of host cytokines and viral genes in the trachea of vaccinated and non-vaccinated chickens after challenge with the infectious laryngotracheitis virus
title_full_unstemmed Evaluation of the transcriptional status of host cytokines and viral genes in the trachea of vaccinated and non-vaccinated chickens after challenge with the infectious laryngotracheitis virus
title_sort Evaluation of the transcriptional status of host cytokines and viral genes in the trachea of vaccinated and non-vaccinated chickens after challenge with the infectious laryngotracheitis virus
dc.creator.none.fl_str_mv Vagnozzi, Ariel Eduardo
Riblet, Sylva
Zavala, Guillermo
Ecco, Roselene
Afonso, Claudio L.
García, Maricarmen
author Vagnozzi, Ariel Eduardo
author_facet Vagnozzi, Ariel Eduardo
Riblet, Sylva
Zavala, Guillermo
Ecco, Roselene
Afonso, Claudio L.
García, Maricarmen
author_role author
author2 Riblet, Sylva
Zavala, Guillermo
Ecco, Roselene
Afonso, Claudio L.
García, Maricarmen
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Laryngotracheitis
Cytokines
Immune Response
Interferons
Chickens
Animal Viruses
Laringotraqueitis
Citoquinas
Respuesta Inmunológica
Interferonas
Pollo
Virus de los Animales
topic Laryngotracheitis
Cytokines
Immune Response
Interferons
Chickens
Animal Viruses
Laringotraqueitis
Citoquinas
Respuesta Inmunológica
Interferonas
Pollo
Virus de los Animales
dc.description.none.fl_txt_mv Infectious laryngotracheitis is a highly contagious disease of chickens responsible for significant economic losses for the poultry industry worldwide. The disease is caused by Gallid herpesvirus-1 (GaHV-1) commonly known as the infectious laryngotracheitis virus. Although characterized by their potential to regain virulence, chicken embryo origin (CEO) vaccines are the most effective vaccines against laryngotracheitis as they significantly reduce the replication of challenge virus in the trachea and conjunctiva. Knowledge on the nature of protective immunity elicited by CEO vaccines is very limited. Therefore, elucidating the origin of the immune responses elicited by CEO vaccination is relevant for development of safer control strategies. In this study the transcription levels of key host immune genes (IFN-γ, IFN-β, IL-1β, IL-6, IL-8, IL-18) and viral genes (ICP4, ICP27, UL46, UL49), as well as viral genome loads in trachea were quantified at 6 and 12 hours post-challenge of CEO vaccinated and non-vaccinated chickens. Immediately after challenge a significant increase in IFN-γ gene expression was followed by a significant reduction in viral replication. In contrast to the rapid induction of IFN-γ, expression of the pro-inflammatory cytokines (IL-1β, IL-6, IL-8) and type I IFN β was either slightly reduced or remained at basal levels. These suggest that the former cytokines may not play important roles during immediate early responses induced by ILTV challenge in either vaccinated or non-vaccinated chickens. Overall, these results suggest that the rapid expression of IFN-γ may induce pathways of antiviral responses necessary for blocking early virus replication.
Instituto de Virología
Fil: Vagnozzi, Ariel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Riblet, Sylva. University of Georgia. College of Veterinary Medicine. Department of Population Health. Poultry Diagnostic and Research Center; Estados Unidos
Fil: Zavala, Guillermo. Avian Health International. Flowery Branch; Estados Unidos
Fil: Ecco, Roselene. Universidade Federal de Minas Gerais. Escola de Veterinária. Laboratorio de Patología; Brasil
Fil: Afonso, Claudio L. Southeast Poultry Research Laboratory; Estados Unidos
Fil: García, Maricarmen. University of Georgia. College of Veterinary Medicine. Department of Population Health. Poultry Diagnostic and Research Center; Estados Unidos
description Infectious laryngotracheitis is a highly contagious disease of chickens responsible for significant economic losses for the poultry industry worldwide. The disease is caused by Gallid herpesvirus-1 (GaHV-1) commonly known as the infectious laryngotracheitis virus. Although characterized by their potential to regain virulence, chicken embryo origin (CEO) vaccines are the most effective vaccines against laryngotracheitis as they significantly reduce the replication of challenge virus in the trachea and conjunctiva. Knowledge on the nature of protective immunity elicited by CEO vaccines is very limited. Therefore, elucidating the origin of the immune responses elicited by CEO vaccination is relevant for development of safer control strategies. In this study the transcription levels of key host immune genes (IFN-γ, IFN-β, IL-1β, IL-6, IL-8, IL-18) and viral genes (ICP4, ICP27, UL46, UL49), as well as viral genome loads in trachea were quantified at 6 and 12 hours post-challenge of CEO vaccinated and non-vaccinated chickens. Immediately after challenge a significant increase in IFN-γ gene expression was followed by a significant reduction in viral replication. In contrast to the rapid induction of IFN-γ, expression of the pro-inflammatory cytokines (IL-1β, IL-6, IL-8) and type I IFN β was either slightly reduced or remained at basal levels. These suggest that the former cytokines may not play important roles during immediate early responses induced by ILTV challenge in either vaccinated or non-vaccinated chickens. Overall, these results suggest that the rapid expression of IFN-γ may induce pathways of antiviral responses necessary for blocking early virus replication.
publishDate 2016
dc.date.none.fl_str_mv 2016
2019-02-13T18:22:15Z
2019-02-13T18:22:15Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4438
0307-9457
1465-3338 (Online)
http://dx.doi.org/10.1080/03079457.2015.1126804
url http://hdl.handle.net/20.500.12123/4438
http://dx.doi.org/10.1080/03079457.2015.1126804
identifier_str_mv 0307-9457
1465-3338 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv Avian pathology 45 (1) : 106–113. (2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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