Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina

Autores
Craig, Marí­a Isabel; Rojas, Marí­a Florencia; Van Der Ploeg, Claudia Andrea; Olivera, Valeria Soledad; Vagnozzi, Ariel Eduardo; Perez, Andrés M.; Konig, Guido Alberto
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Avian infectious laryngotracheitis (ILT) is a worldwide infectious disease that causes important economic losses in the poultry industry. Although it is known that ILT virus (ILTV) is present in Argentina, there is no information about the circulating strains. With the aim to characterize them, seven different genomic regions (thymidine kinase, glycoproteins D, G, B, C, and J, and infected cell polypeptide 4) were partially sequenced and compared between field samples. The gJ sequence resulted to be the most informative segment, it allowed the differentiation among field sample strains, and also, between wild and vaccine viruses. Specific changes in selected nucleotidic positions led to the definition of five distinct haplotypes. Tests for detection of clustering were run to test the null hypothesis that ILTV haplotypes were randomly distributed in time in Argentina and in space in the most densely populated poultry region of this country, Entre Rios. From this study, it was possible to identify a 46 km radius cluster in which higher proportions of haplotypes 4 and 5 were observed, next to a provincial route in Entre Rios and a significant decline of haplotype 5 between 2009 and 2011. Results here provide an update on the molecular epidemiology of ILT in Argentina, including data on specific genome segments that may be used for rapid characterization of the virus in the field. Ultimately, results will contribute to the surveillance of ILT in the country.
Fil: Craig, Marí­a Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Rojas, Marí­a Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio de Sanidad Aviar; Argentina
Fil: Van Der Ploeg, Claudia Andrea. Instituto Nacional de Enfermedades Infecciosas “Dr C. G. Malbrán”; Argentina
Fil: Olivera, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Vagnozzi, Ariel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Perez, Andrés M. University of Minnesota. College of Veterinary Medicine. Department of Veterinary Population Medicine; Estados Unidos
Fil: Konig, Guido Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Frontiers in veterinary science 4. (2017)
Materia
Laringotraqueitis
Virus Bronquitis Infecciosa Aviar
Virus de los Animales
Epidemiología
Glicoproteínas
Genética Molecular
Molecular Genetics
Glycoproteins
Epidemiology
Animal Viruses
Avian Infectious Bronchitis Virus
Laryngotracheitis
Infectious Laryngotracheitis Virus
Spatial Cluster Analysis
Análisis de Cluster Espacial
Argentina
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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oai_identifier_str oai:localhost:20.500.12123/2214
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network_name_str INTA Digital (INTA)
spelling Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from ArgentinaCraig, Marí­a IsabelRojas, Marí­a FlorenciaVan Der Ploeg, Claudia AndreaOlivera, Valeria SoledadVagnozzi, Ariel EduardoPerez, Andrés M.Konig, Guido AlbertoLaringotraqueitisVirus Bronquitis Infecciosa AviarVirus de los AnimalesEpidemiologíaGlicoproteínasGenética MolecularMolecular GeneticsGlycoproteinsEpidemiologyAnimal VirusesAvian Infectious Bronchitis VirusLaryngotracheitisInfectious Laryngotracheitis VirusSpatial Cluster AnalysisAnálisis de Cluster EspacialArgentinaAvian infectious laryngotracheitis (ILT) is a worldwide infectious disease that causes important economic losses in the poultry industry. Although it is known that ILT virus (ILTV) is present in Argentina, there is no information about the circulating strains. With the aim to characterize them, seven different genomic regions (thymidine kinase, glycoproteins D, G, B, C, and J, and infected cell polypeptide 4) were partially sequenced and compared between field samples. The gJ sequence resulted to be the most informative segment, it allowed the differentiation among field sample strains, and also, between wild and vaccine viruses. Specific changes in selected nucleotidic positions led to the definition of five distinct haplotypes. Tests for detection of clustering were run to test the null hypothesis that ILTV haplotypes were randomly distributed in time in Argentina and in space in the most densely populated poultry region of this country, Entre Rios. From this study, it was possible to identify a 46 km radius cluster in which higher proportions of haplotypes 4 and 5 were observed, next to a provincial route in Entre Rios and a significant decline of haplotype 5 between 2009 and 2011. Results here provide an update on the molecular epidemiology of ILT in Argentina, including data on specific genome segments that may be used for rapid characterization of the virus in the field. Ultimately, results will contribute to the surveillance of ILT in the country.Fil: Craig, Marí­a Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Rojas, Marí­a Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio de Sanidad Aviar; ArgentinaFil: Van Der Ploeg, Claudia Andrea. Instituto Nacional de Enfermedades Infecciosas “Dr C. G. Malbrán”; ArgentinaFil: Olivera, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Vagnozzi, Ariel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Perez, Andrés M. University of Minnesota. College of Veterinary Medicine. Department of Veterinary Population Medicine; Estados UnidosFil: Konig, Guido Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina2018-04-10T14:41:14Z2018-04-10T14:41:14Z2017-12-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/2214https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5733342/2297-176910.3389/fvets.2017.00212Frontiers in veterinary science 4. (2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología AgropecuariaengArgentina (nation)info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:29:08Zoai:localhost:20.500.12123/2214instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:09.029INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina
title Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina
spellingShingle Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina
Craig, Marí­a Isabel
Laringotraqueitis
Virus Bronquitis Infecciosa Aviar
Virus de los Animales
Epidemiología
Glicoproteínas
Genética Molecular
Molecular Genetics
Glycoproteins
Epidemiology
Animal Viruses
Avian Infectious Bronchitis Virus
Laryngotracheitis
Infectious Laryngotracheitis Virus
Spatial Cluster Analysis
Análisis de Cluster Espacial
Argentina
title_short Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina
title_full Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina
title_fullStr Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina
title_full_unstemmed Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina
title_sort Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina
dc.creator.none.fl_str_mv Craig, Marí­a Isabel
Rojas, Marí­a Florencia
Van Der Ploeg, Claudia Andrea
Olivera, Valeria Soledad
Vagnozzi, Ariel Eduardo
Perez, Andrés M.
Konig, Guido Alberto
author Craig, Marí­a Isabel
author_facet Craig, Marí­a Isabel
Rojas, Marí­a Florencia
Van Der Ploeg, Claudia Andrea
Olivera, Valeria Soledad
Vagnozzi, Ariel Eduardo
Perez, Andrés M.
Konig, Guido Alberto
author_role author
author2 Rojas, Marí­a Florencia
Van Der Ploeg, Claudia Andrea
Olivera, Valeria Soledad
Vagnozzi, Ariel Eduardo
Perez, Andrés M.
Konig, Guido Alberto
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Laringotraqueitis
Virus Bronquitis Infecciosa Aviar
Virus de los Animales
Epidemiología
Glicoproteínas
Genética Molecular
Molecular Genetics
Glycoproteins
Epidemiology
Animal Viruses
Avian Infectious Bronchitis Virus
Laryngotracheitis
Infectious Laryngotracheitis Virus
Spatial Cluster Analysis
Análisis de Cluster Espacial
Argentina
topic Laringotraqueitis
Virus Bronquitis Infecciosa Aviar
Virus de los Animales
Epidemiología
Glicoproteínas
Genética Molecular
Molecular Genetics
Glycoproteins
Epidemiology
Animal Viruses
Avian Infectious Bronchitis Virus
Laryngotracheitis
Infectious Laryngotracheitis Virus
Spatial Cluster Analysis
Análisis de Cluster Espacial
Argentina
dc.description.none.fl_txt_mv Avian infectious laryngotracheitis (ILT) is a worldwide infectious disease that causes important economic losses in the poultry industry. Although it is known that ILT virus (ILTV) is present in Argentina, there is no information about the circulating strains. With the aim to characterize them, seven different genomic regions (thymidine kinase, glycoproteins D, G, B, C, and J, and infected cell polypeptide 4) were partially sequenced and compared between field samples. The gJ sequence resulted to be the most informative segment, it allowed the differentiation among field sample strains, and also, between wild and vaccine viruses. Specific changes in selected nucleotidic positions led to the definition of five distinct haplotypes. Tests for detection of clustering were run to test the null hypothesis that ILTV haplotypes were randomly distributed in time in Argentina and in space in the most densely populated poultry region of this country, Entre Rios. From this study, it was possible to identify a 46 km radius cluster in which higher proportions of haplotypes 4 and 5 were observed, next to a provincial route in Entre Rios and a significant decline of haplotype 5 between 2009 and 2011. Results here provide an update on the molecular epidemiology of ILT in Argentina, including data on specific genome segments that may be used for rapid characterization of the virus in the field. Ultimately, results will contribute to the surveillance of ILT in the country.
Fil: Craig, Marí­a Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Rojas, Marí­a Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio de Sanidad Aviar; Argentina
Fil: Van Der Ploeg, Claudia Andrea. Instituto Nacional de Enfermedades Infecciosas “Dr C. G. Malbrán”; Argentina
Fil: Olivera, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Vagnozzi, Ariel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Perez, Andrés M. University of Minnesota. College of Veterinary Medicine. Department of Veterinary Population Medicine; Estados Unidos
Fil: Konig, Guido Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Avian infectious laryngotracheitis (ILT) is a worldwide infectious disease that causes important economic losses in the poultry industry. Although it is known that ILT virus (ILTV) is present in Argentina, there is no information about the circulating strains. With the aim to characterize them, seven different genomic regions (thymidine kinase, glycoproteins D, G, B, C, and J, and infected cell polypeptide 4) were partially sequenced and compared between field samples. The gJ sequence resulted to be the most informative segment, it allowed the differentiation among field sample strains, and also, between wild and vaccine viruses. Specific changes in selected nucleotidic positions led to the definition of five distinct haplotypes. Tests for detection of clustering were run to test the null hypothesis that ILTV haplotypes were randomly distributed in time in Argentina and in space in the most densely populated poultry region of this country, Entre Rios. From this study, it was possible to identify a 46 km radius cluster in which higher proportions of haplotypes 4 and 5 were observed, next to a provincial route in Entre Rios and a significant decline of haplotype 5 between 2009 and 2011. Results here provide an update on the molecular epidemiology of ILT in Argentina, including data on specific genome segments that may be used for rapid characterization of the virus in the field. Ultimately, results will contribute to the surveillance of ILT in the country.
publishDate 2017
dc.date.none.fl_str_mv 2017-12-13
2018-04-10T14:41:14Z
2018-04-10T14:41:14Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/2214
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5733342/
2297-1769
10.3389/fvets.2017.00212
url http://hdl.handle.net/20.500.12123/2214
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5733342/
identifier_str_mv 2297-1769
10.3389/fvets.2017.00212
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Argentina (nation)
dc.source.none.fl_str_mv Frontiers in veterinary science 4. (2017)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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