Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina
- Autores
- Craig, María Isabel; Rojas, María Florencia; Van Der Ploeg, Claudia Andrea; Olivera, Valeria Soledad; Vagnozzi, Ariel Eduardo; Perez, Andrés M.; Konig, Guido Alberto
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Avian infectious laryngotracheitis (ILT) is a worldwide infectious disease that causes important economic losses in the poultry industry. Although it is known that ILT virus (ILTV) is present in Argentina, there is no information about the circulating strains. With the aim to characterize them, seven different genomic regions (thymidine kinase, glycoproteins D, G, B, C, and J, and infected cell polypeptide 4) were partially sequenced and compared between field samples. The gJ sequence resulted to be the most informative segment, it allowed the differentiation among field sample strains, and also, between wild and vaccine viruses. Specific changes in selected nucleotidic positions led to the definition of five distinct haplotypes. Tests for detection of clustering were run to test the null hypothesis that ILTV haplotypes were randomly distributed in time in Argentina and in space in the most densely populated poultry region of this country, Entre Rios. From this study, it was possible to identify a 46 km radius cluster in which higher proportions of haplotypes 4 and 5 were observed, next to a provincial route in Entre Rios and a significant decline of haplotype 5 between 2009 and 2011. Results here provide an update on the molecular epidemiology of ILT in Argentina, including data on specific genome segments that may be used for rapid characterization of the virus in the field. Ultimately, results will contribute to the surveillance of ILT in the country.
Fil: Craig, María Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Rojas, María Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio de Sanidad Aviar; Argentina
Fil: Van Der Ploeg, Claudia Andrea. Instituto Nacional de Enfermedades Infecciosas “Dr C. G. Malbrán”; Argentina
Fil: Olivera, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Vagnozzi, Ariel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Perez, Andrés M. University of Minnesota. College of Veterinary Medicine. Department of Veterinary Population Medicine; Estados Unidos
Fil: Konig, Guido Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Fuente
- Frontiers in veterinary science 4. (2017)
- Materia
-
Laringotraqueitis
Virus Bronquitis Infecciosa Aviar
Virus de los Animales
Epidemiología
Glicoproteínas
Genética Molecular
Molecular Genetics
Glycoproteins
Epidemiology
Animal Viruses
Avian Infectious Bronchitis Virus
Laryngotracheitis
Infectious Laryngotracheitis Virus
Spatial Cluster Analysis
Análisis de Cluster Espacial
Argentina - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/2214
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Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from ArgentinaCraig, María IsabelRojas, María FlorenciaVan Der Ploeg, Claudia AndreaOlivera, Valeria SoledadVagnozzi, Ariel EduardoPerez, Andrés M.Konig, Guido AlbertoLaringotraqueitisVirus Bronquitis Infecciosa AviarVirus de los AnimalesEpidemiologíaGlicoproteínasGenética MolecularMolecular GeneticsGlycoproteinsEpidemiologyAnimal VirusesAvian Infectious Bronchitis VirusLaryngotracheitisInfectious Laryngotracheitis VirusSpatial Cluster AnalysisAnálisis de Cluster EspacialArgentinaAvian infectious laryngotracheitis (ILT) is a worldwide infectious disease that causes important economic losses in the poultry industry. Although it is known that ILT virus (ILTV) is present in Argentina, there is no information about the circulating strains. With the aim to characterize them, seven different genomic regions (thymidine kinase, glycoproteins D, G, B, C, and J, and infected cell polypeptide 4) were partially sequenced and compared between field samples. The gJ sequence resulted to be the most informative segment, it allowed the differentiation among field sample strains, and also, between wild and vaccine viruses. Specific changes in selected nucleotidic positions led to the definition of five distinct haplotypes. Tests for detection of clustering were run to test the null hypothesis that ILTV haplotypes were randomly distributed in time in Argentina and in space in the most densely populated poultry region of this country, Entre Rios. From this study, it was possible to identify a 46 km radius cluster in which higher proportions of haplotypes 4 and 5 were observed, next to a provincial route in Entre Rios and a significant decline of haplotype 5 between 2009 and 2011. Results here provide an update on the molecular epidemiology of ILT in Argentina, including data on specific genome segments that may be used for rapid characterization of the virus in the field. Ultimately, results will contribute to the surveillance of ILT in the country.Fil: Craig, María Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Rojas, María Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio de Sanidad Aviar; ArgentinaFil: Van Der Ploeg, Claudia Andrea. Instituto Nacional de Enfermedades Infecciosas “Dr C. G. Malbrán”; ArgentinaFil: Olivera, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Vagnozzi, Ariel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Perez, Andrés M. University of Minnesota. College of Veterinary Medicine. Department of Veterinary Population Medicine; Estados UnidosFil: Konig, Guido Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina2018-04-10T14:41:14Z2018-04-10T14:41:14Z2017-12-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/2214https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5733342/2297-176910.3389/fvets.2017.00212Frontiers in veterinary science 4. (2017)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología AgropecuariaengArgentina (nation)info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:17Zoai:localhost:20.500.12123/2214instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:17.506INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina |
title |
Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina |
spellingShingle |
Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina Craig, María Isabel Laringotraqueitis Virus Bronquitis Infecciosa Aviar Virus de los Animales Epidemiología Glicoproteínas Genética Molecular Molecular Genetics Glycoproteins Epidemiology Animal Viruses Avian Infectious Bronchitis Virus Laryngotracheitis Infectious Laryngotracheitis Virus Spatial Cluster Analysis Análisis de Cluster Espacial Argentina |
title_short |
Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina |
title_full |
Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina |
title_fullStr |
Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina |
title_full_unstemmed |
Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina |
title_sort |
Molecular characterization and cluster analysis of field isolates of avian infectious laryngotracheitis virus from Argentina |
dc.creator.none.fl_str_mv |
Craig, María Isabel Rojas, María Florencia Van Der Ploeg, Claudia Andrea Olivera, Valeria Soledad Vagnozzi, Ariel Eduardo Perez, Andrés M. Konig, Guido Alberto |
author |
Craig, María Isabel |
author_facet |
Craig, María Isabel Rojas, María Florencia Van Der Ploeg, Claudia Andrea Olivera, Valeria Soledad Vagnozzi, Ariel Eduardo Perez, Andrés M. Konig, Guido Alberto |
author_role |
author |
author2 |
Rojas, María Florencia Van Der Ploeg, Claudia Andrea Olivera, Valeria Soledad Vagnozzi, Ariel Eduardo Perez, Andrés M. Konig, Guido Alberto |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
Laringotraqueitis Virus Bronquitis Infecciosa Aviar Virus de los Animales Epidemiología Glicoproteínas Genética Molecular Molecular Genetics Glycoproteins Epidemiology Animal Viruses Avian Infectious Bronchitis Virus Laryngotracheitis Infectious Laryngotracheitis Virus Spatial Cluster Analysis Análisis de Cluster Espacial Argentina |
topic |
Laringotraqueitis Virus Bronquitis Infecciosa Aviar Virus de los Animales Epidemiología Glicoproteínas Genética Molecular Molecular Genetics Glycoproteins Epidemiology Animal Viruses Avian Infectious Bronchitis Virus Laryngotracheitis Infectious Laryngotracheitis Virus Spatial Cluster Analysis Análisis de Cluster Espacial Argentina |
dc.description.none.fl_txt_mv |
Avian infectious laryngotracheitis (ILT) is a worldwide infectious disease that causes important economic losses in the poultry industry. Although it is known that ILT virus (ILTV) is present in Argentina, there is no information about the circulating strains. With the aim to characterize them, seven different genomic regions (thymidine kinase, glycoproteins D, G, B, C, and J, and infected cell polypeptide 4) were partially sequenced and compared between field samples. The gJ sequence resulted to be the most informative segment, it allowed the differentiation among field sample strains, and also, between wild and vaccine viruses. Specific changes in selected nucleotidic positions led to the definition of five distinct haplotypes. Tests for detection of clustering were run to test the null hypothesis that ILTV haplotypes were randomly distributed in time in Argentina and in space in the most densely populated poultry region of this country, Entre Rios. From this study, it was possible to identify a 46 km radius cluster in which higher proportions of haplotypes 4 and 5 were observed, next to a provincial route in Entre Rios and a significant decline of haplotype 5 between 2009 and 2011. Results here provide an update on the molecular epidemiology of ILT in Argentina, including data on specific genome segments that may be used for rapid characterization of the virus in the field. Ultimately, results will contribute to the surveillance of ILT in the country. Fil: Craig, María Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Rojas, María Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay. Laboratorio de Sanidad Aviar; Argentina Fil: Van Der Ploeg, Claudia Andrea. Instituto Nacional de Enfermedades Infecciosas “Dr C. G. Malbrán”; Argentina Fil: Olivera, Valeria Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Vagnozzi, Ariel Eduardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina Fil: Perez, Andrés M. University of Minnesota. College of Veterinary Medicine. Department of Veterinary Population Medicine; Estados Unidos Fil: Konig, Guido Alberto. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
Avian infectious laryngotracheitis (ILT) is a worldwide infectious disease that causes important economic losses in the poultry industry. Although it is known that ILT virus (ILTV) is present in Argentina, there is no information about the circulating strains. With the aim to characterize them, seven different genomic regions (thymidine kinase, glycoproteins D, G, B, C, and J, and infected cell polypeptide 4) were partially sequenced and compared between field samples. The gJ sequence resulted to be the most informative segment, it allowed the differentiation among field sample strains, and also, between wild and vaccine viruses. Specific changes in selected nucleotidic positions led to the definition of five distinct haplotypes. Tests for detection of clustering were run to test the null hypothesis that ILTV haplotypes were randomly distributed in time in Argentina and in space in the most densely populated poultry region of this country, Entre Rios. From this study, it was possible to identify a 46 km radius cluster in which higher proportions of haplotypes 4 and 5 were observed, next to a provincial route in Entre Rios and a significant decline of haplotype 5 between 2009 and 2011. Results here provide an update on the molecular epidemiology of ILT in Argentina, including data on specific genome segments that may be used for rapid characterization of the virus in the field. Ultimately, results will contribute to the surveillance of ILT in the country. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-13 2018-04-10T14:41:14Z 2018-04-10T14:41:14Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/2214 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5733342/ 2297-1769 10.3389/fvets.2017.00212 |
url |
http://hdl.handle.net/20.500.12123/2214 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5733342/ |
identifier_str_mv |
2297-1769 10.3389/fvets.2017.00212 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.coverage.none.fl_str_mv |
Argentina (nation) |
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Frontiers in veterinary science 4. (2017) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) |
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INTA Digital (INTA) |
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Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria |
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tripaldi.nicolas@inta.gob.ar |
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