Age-related lung cell response to urban Buenos Aires air particle soluble fraction

Autores
Ostachuk, Agustin Ignacio; Evelson, Pablo Andrés; Martin, Susana; Dawidowski, Laura Elena; Yakisich, J. Sebastián; Tasat, Deborah Ruth
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Exposure to particulate matter (PM) may alter lung homeostasis inducing changes in fluid balance and host defense. Bioavailability of soluble PM compounds like polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and transition metals has been shown to play a key role in lung injury. We have previously characterized the size, shape, and chemical components of urban air particles from Buenos Aires (UAP-BA) and their biological impact on lungs. Herein, we evaluate the possible toxic effect of UAP-BA-soluble fraction (UAP-BAsf) on pulmonary cells obtained from young (1-2 months old) and aged (9-12 months old) Wistar rats using phagocytosis, oxidant-antioxidant generation, and apoptosis as endpoints. UAP-BA were collected in downtown BA and residual oil fly ash (ROFA), employed as a positive control, was collected from Boston Edison Co., Mystic Power Plant, Mystic, CT, USA. Both particle-soluble fractions (sf) were employed at concentrations ranging from 0 to 100 microg/mL. UAP-BAsf and ROFAsf even at the lowest dose assayed (10 microg/mL) showed in both lung cell populations the ability to stimulate phagocytosis and increase superoxide anion (O(2)(-)) generation. Both types of air particles caused a marked intracellular oxidant stress in aged pulmonary cells that may contribute to subsequent cell activation and production of proinflammatory mediators, leading to cell dysfunction. These data suggest that the impact of UAP-BAsf on phagocytosis, oxidant radical generation, and apoptosis is clearly dependent on the maturational state of the animal and might have different mechanisms of action.
Fil: Ostachuk, Agustin Ignacio. Universidad Nacional de San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Evelson, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Martin, Susana. Universidad Nacional de San Martín; Argentina
Fil: Dawidowski, Laura Elena. Comisión Nacional de Energía Atómica; Argentina
Fil: Yakisich, J. Sebastián. Texas A&M University; Estados Unidos
Fil: Tasat, Deborah Ruth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Materia
AIR POLLUTION
PARTICULATE MATTER
SOLUBLE FRACTION
AGING
LUNG
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/141875
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/141875
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Age-related lung cell response to urban Buenos Aires air particle soluble fractionOstachuk, Agustin IgnacioEvelson, Pablo AndrésMartin, SusanaDawidowski, Laura ElenaYakisich, J. SebastiánTasat, Deborah RuthAIR POLLUTIONPARTICULATE MATTERSOLUBLE FRACTIONAGINGLUNGhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Exposure to particulate matter (PM) may alter lung homeostasis inducing changes in fluid balance and host defense. Bioavailability of soluble PM compounds like polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and transition metals has been shown to play a key role in lung injury. We have previously characterized the size, shape, and chemical components of urban air particles from Buenos Aires (UAP-BA) and their biological impact on lungs. Herein, we evaluate the possible toxic effect of UAP-BA-soluble fraction (UAP-BAsf) on pulmonary cells obtained from young (1-2 months old) and aged (9-12 months old) Wistar rats using phagocytosis, oxidant-antioxidant generation, and apoptosis as endpoints. UAP-BA were collected in downtown BA and residual oil fly ash (ROFA), employed as a positive control, was collected from Boston Edison Co., Mystic Power Plant, Mystic, CT, USA. Both particle-soluble fractions (sf) were employed at concentrations ranging from 0 to 100 microg/mL. UAP-BAsf and ROFAsf even at the lowest dose assayed (10 microg/mL) showed in both lung cell populations the ability to stimulate phagocytosis and increase superoxide anion (O(2)(-)) generation. Both types of air particles caused a marked intracellular oxidant stress in aged pulmonary cells that may contribute to subsequent cell activation and production of proinflammatory mediators, leading to cell dysfunction. These data suggest that the impact of UAP-BAsf on phagocytosis, oxidant radical generation, and apoptosis is clearly dependent on the maturational state of the animal and might have different mechanisms of action.Fil: Ostachuk, Agustin Ignacio. Universidad Nacional de San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Evelson, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Martin, Susana. Universidad Nacional de San Martín; ArgentinaFil: Dawidowski, Laura Elena. Comisión Nacional de Energía Atómica; ArgentinaFil: Yakisich, J. Sebastián. Texas A&M University; Estados UnidosFil: Tasat, Deborah Ruth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaAcademic Press Inc Elsevier Science2008-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/141875Ostachuk, Agustin Ignacio; Evelson, Pablo Andrés; Martin, Susana; Dawidowski, Laura Elena; Yakisich, J. Sebastián; et al.; Age-related lung cell response to urban Buenos Aires air particle soluble fraction; Academic Press Inc Elsevier Science; Environmental Research; 107; 2; 6-2008; 170-1770013-9351CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.envres.2008.01.007info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0013935108000200info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T10:56:52Zoai:ri.conicet.gov.ar:11336/141875instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 10:56:52.553CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Age-related lung cell response to urban Buenos Aires air particle soluble fraction
title Age-related lung cell response to urban Buenos Aires air particle soluble fraction
spellingShingle Age-related lung cell response to urban Buenos Aires air particle soluble fraction
Ostachuk, Agustin Ignacio
AIR POLLUTION
PARTICULATE MATTER
SOLUBLE FRACTION
AGING
LUNG
title_short Age-related lung cell response to urban Buenos Aires air particle soluble fraction
title_full Age-related lung cell response to urban Buenos Aires air particle soluble fraction
title_fullStr Age-related lung cell response to urban Buenos Aires air particle soluble fraction
title_full_unstemmed Age-related lung cell response to urban Buenos Aires air particle soluble fraction
title_sort Age-related lung cell response to urban Buenos Aires air particle soluble fraction
dc.creator.none.fl_str_mv Ostachuk, Agustin Ignacio
Evelson, Pablo Andrés
Martin, Susana
Dawidowski, Laura Elena
Yakisich, J. Sebastián
Tasat, Deborah Ruth
author Ostachuk, Agustin Ignacio
author_facet Ostachuk, Agustin Ignacio
Evelson, Pablo Andrés
Martin, Susana
Dawidowski, Laura Elena
Yakisich, J. Sebastián
Tasat, Deborah Ruth
author_role author
author2 Evelson, Pablo Andrés
Martin, Susana
Dawidowski, Laura Elena
Yakisich, J. Sebastián
Tasat, Deborah Ruth
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv AIR POLLUTION
PARTICULATE MATTER
SOLUBLE FRACTION
AGING
LUNG
topic AIR POLLUTION
PARTICULATE MATTER
SOLUBLE FRACTION
AGING
LUNG
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Exposure to particulate matter (PM) may alter lung homeostasis inducing changes in fluid balance and host defense. Bioavailability of soluble PM compounds like polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and transition metals has been shown to play a key role in lung injury. We have previously characterized the size, shape, and chemical components of urban air particles from Buenos Aires (UAP-BA) and their biological impact on lungs. Herein, we evaluate the possible toxic effect of UAP-BA-soluble fraction (UAP-BAsf) on pulmonary cells obtained from young (1-2 months old) and aged (9-12 months old) Wistar rats using phagocytosis, oxidant-antioxidant generation, and apoptosis as endpoints. UAP-BA were collected in downtown BA and residual oil fly ash (ROFA), employed as a positive control, was collected from Boston Edison Co., Mystic Power Plant, Mystic, CT, USA. Both particle-soluble fractions (sf) were employed at concentrations ranging from 0 to 100 microg/mL. UAP-BAsf and ROFAsf even at the lowest dose assayed (10 microg/mL) showed in both lung cell populations the ability to stimulate phagocytosis and increase superoxide anion (O(2)(-)) generation. Both types of air particles caused a marked intracellular oxidant stress in aged pulmonary cells that may contribute to subsequent cell activation and production of proinflammatory mediators, leading to cell dysfunction. These data suggest that the impact of UAP-BAsf on phagocytosis, oxidant radical generation, and apoptosis is clearly dependent on the maturational state of the animal and might have different mechanisms of action.
Fil: Ostachuk, Agustin Ignacio. Universidad Nacional de San Martín; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Evelson, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Programa de Radicales Libres; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Martin, Susana. Universidad Nacional de San Martín; Argentina
Fil: Dawidowski, Laura Elena. Comisión Nacional de Energía Atómica; Argentina
Fil: Yakisich, J. Sebastián. Texas A&M University; Estados Unidos
Fil: Tasat, Deborah Ruth. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
description Exposure to particulate matter (PM) may alter lung homeostasis inducing changes in fluid balance and host defense. Bioavailability of soluble PM compounds like polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and transition metals has been shown to play a key role in lung injury. We have previously characterized the size, shape, and chemical components of urban air particles from Buenos Aires (UAP-BA) and their biological impact on lungs. Herein, we evaluate the possible toxic effect of UAP-BA-soluble fraction (UAP-BAsf) on pulmonary cells obtained from young (1-2 months old) and aged (9-12 months old) Wistar rats using phagocytosis, oxidant-antioxidant generation, and apoptosis as endpoints. UAP-BA were collected in downtown BA and residual oil fly ash (ROFA), employed as a positive control, was collected from Boston Edison Co., Mystic Power Plant, Mystic, CT, USA. Both particle-soluble fractions (sf) were employed at concentrations ranging from 0 to 100 microg/mL. UAP-BAsf and ROFAsf even at the lowest dose assayed (10 microg/mL) showed in both lung cell populations the ability to stimulate phagocytosis and increase superoxide anion (O(2)(-)) generation. Both types of air particles caused a marked intracellular oxidant stress in aged pulmonary cells that may contribute to subsequent cell activation and production of proinflammatory mediators, leading to cell dysfunction. These data suggest that the impact of UAP-BAsf on phagocytosis, oxidant radical generation, and apoptosis is clearly dependent on the maturational state of the animal and might have different mechanisms of action.
publishDate 2008
dc.date.none.fl_str_mv 2008-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/141875
Ostachuk, Agustin Ignacio; Evelson, Pablo Andrés; Martin, Susana; Dawidowski, Laura Elena; Yakisich, J. Sebastián; et al.; Age-related lung cell response to urban Buenos Aires air particle soluble fraction; Academic Press Inc Elsevier Science; Environmental Research; 107; 2; 6-2008; 170-177
0013-9351
CONICET Digital
CONICET
url http://hdl.handle.net/11336/141875
identifier_str_mv Ostachuk, Agustin Ignacio; Evelson, Pablo Andrés; Martin, Susana; Dawidowski, Laura Elena; Yakisich, J. Sebastián; et al.; Age-related lung cell response to urban Buenos Aires air particle soluble fraction; Academic Press Inc Elsevier Science; Environmental Research; 107; 2; 6-2008; 170-177
0013-9351
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.envres.2008.01.007
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0013935108000200
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.001348

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