Air particulate matter exacerbates lung response on Sjögren's Syndrome animals

Autores
Ferraro, Sebastián Ariel; Orona, Nadia Soledad; Villalon, Liliana; do Nascimento Saldiva, Paulo Hilário; Tasat, Deborah Ruth; Berra, Alejandro
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Epidemiological studies have associated air particulate matter (PM) inhalation with a decline in lung function and increased morbo-mortality due to cardiorespiratory diseases, particularly in susceptible populations. Sjögren´s Syndrome (SS) is a chronic autoimmune disease characterized by cellular infiltration in exocrine glands and extraglandular tissue, being the respiratory tract an important target. We evaluated the effect of PM on the airways of NOD mice, which develop SS and BALB/c mice. BALB/c or NOD mice (2-3 months) were randomized in two groups and exposed to intranasal instillation either with saline (control) or ROFA solution (1mg/kg body weight). After 24h, mice were euthanized in order to perform lung histology, or measure total cell number (TCN), differential cell count (DCC) and superoxide anion generation in the bronchoalveolar lavage (BAL) fluid. BALB/c mice showed normal histoarchitecture, while NOD mice showed lymphocytic peribronchial infiltrates. ROFA exposure affected the respiratory tract from both BALB/c and NOD mice, with a significant increase in the TCN (p<0.05) and generation of O2- (p<0.05), as well as an imbalance in the DCC (p<0.05). All histological observations correlated with the cellular parameters evaluated. Lesions in NOD mice were more severe than those of BALB/c, showing cellular infiltration in the alveoli and leading to a greater decrease in the alveolar space. We have proved that in this experimental Sjögren´s Syndrome animal model (NOD mice); airborne pollution exacerbates pre-existing pulmonary lesions. These findings show experimental evidence on the harmful effects of airborne pollution on the airways of patients with Sjögren´s Syndrome.
Fil: Ferraro, Sebastián Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Fil: Orona, Nadia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Fil: Villalon, Liliana. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología; Argentina
Fil: do Nascimento Saldiva, Paulo Hilário. National Research Council; Brasil
Fil: Tasat, Deborah Ruth. Universidad de Buenos Aires; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Fil: Berra, Alejandro. Universidad de Buenos Aires; Argentina
Materia
Lung
Particulate Matter
Inflammation
Sjögren Syndrome
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/36504
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/36504
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Air particulate matter exacerbates lung response on Sjögren's Syndrome animalsFerraro, Sebastián ArielOrona, Nadia SoledadVillalon, Lilianado Nascimento Saldiva, Paulo HilárioTasat, Deborah RuthBerra, AlejandroLungParticulate MatterInflammationSjögren Syndromehttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Epidemiological studies have associated air particulate matter (PM) inhalation with a decline in lung function and increased morbo-mortality due to cardiorespiratory diseases, particularly in susceptible populations. Sjögren´s Syndrome (SS) is a chronic autoimmune disease characterized by cellular infiltration in exocrine glands and extraglandular tissue, being the respiratory tract an important target. We evaluated the effect of PM on the airways of NOD mice, which develop SS and BALB/c mice. BALB/c or NOD mice (2-3 months) were randomized in two groups and exposed to intranasal instillation either with saline (control) or ROFA solution (1mg/kg body weight). After 24h, mice were euthanized in order to perform lung histology, or measure total cell number (TCN), differential cell count (DCC) and superoxide anion generation in the bronchoalveolar lavage (BAL) fluid. BALB/c mice showed normal histoarchitecture, while NOD mice showed lymphocytic peribronchial infiltrates. ROFA exposure affected the respiratory tract from both BALB/c and NOD mice, with a significant increase in the TCN (p<0.05) and generation of O2- (p<0.05), as well as an imbalance in the DCC (p<0.05). All histological observations correlated with the cellular parameters evaluated. Lesions in NOD mice were more severe than those of BALB/c, showing cellular infiltration in the alveoli and leading to a greater decrease in the alveolar space. We have proved that in this experimental Sjögren´s Syndrome animal model (NOD mice); airborne pollution exacerbates pre-existing pulmonary lesions. These findings show experimental evidence on the harmful effects of airborne pollution on the airways of patients with Sjögren´s Syndrome.Fil: Ferraro, Sebastián Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; ArgentinaFil: Orona, Nadia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; ArgentinaFil: Villalon, Liliana. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología; ArgentinaFil: do Nascimento Saldiva, Paulo Hilário. National Research Council; BrasilFil: Tasat, Deborah Ruth. Universidad de Buenos Aires; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; ArgentinaFil: Berra, Alejandro. Universidad de Buenos Aires; ArgentinaElsevier Gmbh2014-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/36504Ferraro, Sebastián Ariel; Orona, Nadia Soledad; Villalon, Liliana; do Nascimento Saldiva, Paulo Hilário; Tasat, Deborah Ruth; et al.; Air particulate matter exacerbates lung response on Sjögren's Syndrome animals; Elsevier Gmbh; Experimental And Toxicologic Pathology; 67; 11-2014; 125-1310940-2993CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/ 10.1016/j.etp.2014.10.007info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0940299314001493info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:52:53Zoai:ri.conicet.gov.ar:11336/36504instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:52:54.226CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Air particulate matter exacerbates lung response on Sjögren's Syndrome animals
title Air particulate matter exacerbates lung response on Sjögren's Syndrome animals
spellingShingle Air particulate matter exacerbates lung response on Sjögren's Syndrome animals
Ferraro, Sebastián Ariel
Lung
Particulate Matter
Inflammation
Sjögren Syndrome
title_short Air particulate matter exacerbates lung response on Sjögren's Syndrome animals
title_full Air particulate matter exacerbates lung response on Sjögren's Syndrome animals
title_fullStr Air particulate matter exacerbates lung response on Sjögren's Syndrome animals
title_full_unstemmed Air particulate matter exacerbates lung response on Sjögren's Syndrome animals
title_sort Air particulate matter exacerbates lung response on Sjögren's Syndrome animals
dc.creator.none.fl_str_mv Ferraro, Sebastián Ariel
Orona, Nadia Soledad
Villalon, Liliana
do Nascimento Saldiva, Paulo Hilário
Tasat, Deborah Ruth
Berra, Alejandro
author Ferraro, Sebastián Ariel
author_facet Ferraro, Sebastián Ariel
Orona, Nadia Soledad
Villalon, Liliana
do Nascimento Saldiva, Paulo Hilário
Tasat, Deborah Ruth
Berra, Alejandro
author_role author
author2 Orona, Nadia Soledad
Villalon, Liliana
do Nascimento Saldiva, Paulo Hilário
Tasat, Deborah Ruth
Berra, Alejandro
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Lung
Particulate Matter
Inflammation
Sjögren Syndrome
topic Lung
Particulate Matter
Inflammation
Sjögren Syndrome
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Epidemiological studies have associated air particulate matter (PM) inhalation with a decline in lung function and increased morbo-mortality due to cardiorespiratory diseases, particularly in susceptible populations. Sjögren´s Syndrome (SS) is a chronic autoimmune disease characterized by cellular infiltration in exocrine glands and extraglandular tissue, being the respiratory tract an important target. We evaluated the effect of PM on the airways of NOD mice, which develop SS and BALB/c mice. BALB/c or NOD mice (2-3 months) were randomized in two groups and exposed to intranasal instillation either with saline (control) or ROFA solution (1mg/kg body weight). After 24h, mice were euthanized in order to perform lung histology, or measure total cell number (TCN), differential cell count (DCC) and superoxide anion generation in the bronchoalveolar lavage (BAL) fluid. BALB/c mice showed normal histoarchitecture, while NOD mice showed lymphocytic peribronchial infiltrates. ROFA exposure affected the respiratory tract from both BALB/c and NOD mice, with a significant increase in the TCN (p<0.05) and generation of O2- (p<0.05), as well as an imbalance in the DCC (p<0.05). All histological observations correlated with the cellular parameters evaluated. Lesions in NOD mice were more severe than those of BALB/c, showing cellular infiltration in the alveoli and leading to a greater decrease in the alveolar space. We have proved that in this experimental Sjögren´s Syndrome animal model (NOD mice); airborne pollution exacerbates pre-existing pulmonary lesions. These findings show experimental evidence on the harmful effects of airborne pollution on the airways of patients with Sjögren´s Syndrome.
Fil: Ferraro, Sebastián Ariel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Fil: Orona, Nadia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Fil: Villalon, Liliana. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Patología; Argentina
Fil: do Nascimento Saldiva, Paulo Hilário. National Research Council; Brasil
Fil: Tasat, Deborah Ruth. Universidad de Buenos Aires; Argentina. Universidad Nacional de San Martín. Escuela de Ciencia y Tecnología; Argentina
Fil: Berra, Alejandro. Universidad de Buenos Aires; Argentina
description Epidemiological studies have associated air particulate matter (PM) inhalation with a decline in lung function and increased morbo-mortality due to cardiorespiratory diseases, particularly in susceptible populations. Sjögren´s Syndrome (SS) is a chronic autoimmune disease characterized by cellular infiltration in exocrine glands and extraglandular tissue, being the respiratory tract an important target. We evaluated the effect of PM on the airways of NOD mice, which develop SS and BALB/c mice. BALB/c or NOD mice (2-3 months) were randomized in two groups and exposed to intranasal instillation either with saline (control) or ROFA solution (1mg/kg body weight). After 24h, mice were euthanized in order to perform lung histology, or measure total cell number (TCN), differential cell count (DCC) and superoxide anion generation in the bronchoalveolar lavage (BAL) fluid. BALB/c mice showed normal histoarchitecture, while NOD mice showed lymphocytic peribronchial infiltrates. ROFA exposure affected the respiratory tract from both BALB/c and NOD mice, with a significant increase in the TCN (p<0.05) and generation of O2- (p<0.05), as well as an imbalance in the DCC (p<0.05). All histological observations correlated with the cellular parameters evaluated. Lesions in NOD mice were more severe than those of BALB/c, showing cellular infiltration in the alveoli and leading to a greater decrease in the alveolar space. We have proved that in this experimental Sjögren´s Syndrome animal model (NOD mice); airborne pollution exacerbates pre-existing pulmonary lesions. These findings show experimental evidence on the harmful effects of airborne pollution on the airways of patients with Sjögren´s Syndrome.
publishDate 2014
dc.date.none.fl_str_mv 2014-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/36504
Ferraro, Sebastián Ariel; Orona, Nadia Soledad; Villalon, Liliana; do Nascimento Saldiva, Paulo Hilário; Tasat, Deborah Ruth; et al.; Air particulate matter exacerbates lung response on Sjögren's Syndrome animals; Elsevier Gmbh; Experimental And Toxicologic Pathology; 67; 11-2014; 125-131
0940-2993
CONICET Digital
CONICET
url http://hdl.handle.net/11336/36504
identifier_str_mv Ferraro, Sebastián Ariel; Orona, Nadia Soledad; Villalon, Liliana; do Nascimento Saldiva, Paulo Hilário; Tasat, Deborah Ruth; et al.; Air particulate matter exacerbates lung response on Sjögren's Syndrome animals; Elsevier Gmbh; Experimental And Toxicologic Pathology; 67; 11-2014; 125-131
0940-2993
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/ 10.1016/j.etp.2014.10.007
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0940299314001493
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Gmbh
publisher.none.fl_str_mv Elsevier Gmbh
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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