Keratinocytes oxidative damage mechanisms related to airbone particle matter exposure

Autores
Romani, Arianna; Cervellati, Carlo; Muresan, Ximena M.; Belmonte, Giuseppe; Pecorelli, Alessandra; Cervellati, Franco; Benedusi, Mascia; Evelson, Pablo Andrés; Valacchi, Giuseppe
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Epidemiological evidences have correlated airbone particulate matter (PM) to adverse health effects, mainly linking to pulmonary and cardiovascular disease. Nevertheless, only recently, some studies reported detrimental effects of PM on other organs such as skin. In a recent work, we have reported increased oxidative and inflammatory responses in Reconstituted Human Epidermis (RHE) exposed to ambient particles (CAPs) and we also demonstrated the ability of CAPs to penetrate the skin tissue.The present study was aimed to better understand the cellular mechanisms beyond the oxidative changes induced by CAPs (5-10-25. μg/mL) in human immortalized keratinocytes (HaCaT).After 24. h of treatment, CAPs were able to enter the cells leading to a decrease in viability, increased levels of 4-hydroxinonenal products (4-HNE) and IL-1α release. Overall these data, suggest lipid and protein oxidative damage, as well as an increase of inflammatory response after being challenged with CAPs. In addition, 3. h after CAPs exposure we found a significant increase in NF-kB and Nrf2 translocation into the nucleus. In contrast, no differences in gene expression and enzymatic activity of Nrf2 target genes were detected. This last finding could be explained by the ability of CAPs to possibly alter the binding of Nrf2 to the ARE DNA sequence.
Fil: Romani, Arianna. Università di Ferrara; Italia
Fil: Cervellati, Carlo. Università di Ferrara; Italia
Fil: Muresan, Ximena M.. Università di Ferrara; Italia
Fil: Belmonte, Giuseppe. Università di Ferrara; Italia
Fil: Pecorelli, Alessandra. North Carolina State University; Estados Unidos
Fil: Cervellati, Franco. Università di Ferrara; Italia
Fil: Benedusi, Mascia. Università di Ferrara; Italia
Fil: Evelson, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular. Departamento de Patología; Argentina
Fil: Valacchi, Giuseppe. Università di Ferrara; Italia. North Carolina State University; Estados Unidos
Materia
AIR POLLUTION
INFLAMMATION
OXIDATIVE DAMAGE
PARTICULATE MATTER
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47421
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/47421
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Keratinocytes oxidative damage mechanisms related to airbone particle matter exposureRomani, AriannaCervellati, CarloMuresan, Ximena M.Belmonte, GiuseppePecorelli, AlessandraCervellati, FrancoBenedusi, MasciaEvelson, Pablo AndrésValacchi, GiuseppeAIR POLLUTIONINFLAMMATIONOXIDATIVE DAMAGEPARTICULATE MATTERhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Epidemiological evidences have correlated airbone particulate matter (PM) to adverse health effects, mainly linking to pulmonary and cardiovascular disease. Nevertheless, only recently, some studies reported detrimental effects of PM on other organs such as skin. In a recent work, we have reported increased oxidative and inflammatory responses in Reconstituted Human Epidermis (RHE) exposed to ambient particles (CAPs) and we also demonstrated the ability of CAPs to penetrate the skin tissue.The present study was aimed to better understand the cellular mechanisms beyond the oxidative changes induced by CAPs (5-10-25. μg/mL) in human immortalized keratinocytes (HaCaT).After 24. h of treatment, CAPs were able to enter the cells leading to a decrease in viability, increased levels of 4-hydroxinonenal products (4-HNE) and IL-1α release. Overall these data, suggest lipid and protein oxidative damage, as well as an increase of inflammatory response after being challenged with CAPs. In addition, 3. h after CAPs exposure we found a significant increase in NF-kB and Nrf2 translocation into the nucleus. In contrast, no differences in gene expression and enzymatic activity of Nrf2 target genes were detected. This last finding could be explained by the ability of CAPs to possibly alter the binding of Nrf2 to the ARE DNA sequence.Fil: Romani, Arianna. Università di Ferrara; ItaliaFil: Cervellati, Carlo. Università di Ferrara; ItaliaFil: Muresan, Ximena M.. Università di Ferrara; ItaliaFil: Belmonte, Giuseppe. Università di Ferrara; ItaliaFil: Pecorelli, Alessandra. North Carolina State University; Estados UnidosFil: Cervellati, Franco. Università di Ferrara; ItaliaFil: Benedusi, Mascia. Università di Ferrara; ItaliaFil: Evelson, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular. Departamento de Patología; ArgentinaFil: Valacchi, Giuseppe. Università di Ferrara; Italia. North Carolina State University; Estados UnidosElsevier Ireland2017-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47421Romani, Arianna; Cervellati, Carlo; Muresan, Ximena M.; Belmonte, Giuseppe; Pecorelli, Alessandra; et al.; Keratinocytes oxidative damage mechanisms related to airbone particle matter exposure; Elsevier Ireland; Mechanisms of Ageing and Development; 172; 11-2017; 86-950047-6374CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.mad.2017.11.007info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0047637417300830info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:27:57Zoai:ri.conicet.gov.ar:11336/47421instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:27:57.609CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Keratinocytes oxidative damage mechanisms related to airbone particle matter exposure
title Keratinocytes oxidative damage mechanisms related to airbone particle matter exposure
spellingShingle Keratinocytes oxidative damage mechanisms related to airbone particle matter exposure
Romani, Arianna
AIR POLLUTION
INFLAMMATION
OXIDATIVE DAMAGE
PARTICULATE MATTER
title_short Keratinocytes oxidative damage mechanisms related to airbone particle matter exposure
title_full Keratinocytes oxidative damage mechanisms related to airbone particle matter exposure
title_fullStr Keratinocytes oxidative damage mechanisms related to airbone particle matter exposure
title_full_unstemmed Keratinocytes oxidative damage mechanisms related to airbone particle matter exposure
title_sort Keratinocytes oxidative damage mechanisms related to airbone particle matter exposure
dc.creator.none.fl_str_mv Romani, Arianna
Cervellati, Carlo
Muresan, Ximena M.
Belmonte, Giuseppe
Pecorelli, Alessandra
Cervellati, Franco
Benedusi, Mascia
Evelson, Pablo Andrés
Valacchi, Giuseppe
author Romani, Arianna
author_facet Romani, Arianna
Cervellati, Carlo
Muresan, Ximena M.
Belmonte, Giuseppe
Pecorelli, Alessandra
Cervellati, Franco
Benedusi, Mascia
Evelson, Pablo Andrés
Valacchi, Giuseppe
author_role author
author2 Cervellati, Carlo
Muresan, Ximena M.
Belmonte, Giuseppe
Pecorelli, Alessandra
Cervellati, Franco
Benedusi, Mascia
Evelson, Pablo Andrés
Valacchi, Giuseppe
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AIR POLLUTION
INFLAMMATION
OXIDATIVE DAMAGE
PARTICULATE MATTER
topic AIR POLLUTION
INFLAMMATION
OXIDATIVE DAMAGE
PARTICULATE MATTER
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Epidemiological evidences have correlated airbone particulate matter (PM) to adverse health effects, mainly linking to pulmonary and cardiovascular disease. Nevertheless, only recently, some studies reported detrimental effects of PM on other organs such as skin. In a recent work, we have reported increased oxidative and inflammatory responses in Reconstituted Human Epidermis (RHE) exposed to ambient particles (CAPs) and we also demonstrated the ability of CAPs to penetrate the skin tissue.The present study was aimed to better understand the cellular mechanisms beyond the oxidative changes induced by CAPs (5-10-25. μg/mL) in human immortalized keratinocytes (HaCaT).After 24. h of treatment, CAPs were able to enter the cells leading to a decrease in viability, increased levels of 4-hydroxinonenal products (4-HNE) and IL-1α release. Overall these data, suggest lipid and protein oxidative damage, as well as an increase of inflammatory response after being challenged with CAPs. In addition, 3. h after CAPs exposure we found a significant increase in NF-kB and Nrf2 translocation into the nucleus. In contrast, no differences in gene expression and enzymatic activity of Nrf2 target genes were detected. This last finding could be explained by the ability of CAPs to possibly alter the binding of Nrf2 to the ARE DNA sequence.
Fil: Romani, Arianna. Università di Ferrara; Italia
Fil: Cervellati, Carlo. Università di Ferrara; Italia
Fil: Muresan, Ximena M.. Università di Ferrara; Italia
Fil: Belmonte, Giuseppe. Università di Ferrara; Italia
Fil: Pecorelli, Alessandra. North Carolina State University; Estados Unidos
Fil: Cervellati, Franco. Università di Ferrara; Italia
Fil: Benedusi, Mascia. Università di Ferrara; Italia
Fil: Evelson, Pablo Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular. Departamento de Patología; Argentina
Fil: Valacchi, Giuseppe. Università di Ferrara; Italia. North Carolina State University; Estados Unidos
description Epidemiological evidences have correlated airbone particulate matter (PM) to adverse health effects, mainly linking to pulmonary and cardiovascular disease. Nevertheless, only recently, some studies reported detrimental effects of PM on other organs such as skin. In a recent work, we have reported increased oxidative and inflammatory responses in Reconstituted Human Epidermis (RHE) exposed to ambient particles (CAPs) and we also demonstrated the ability of CAPs to penetrate the skin tissue.The present study was aimed to better understand the cellular mechanisms beyond the oxidative changes induced by CAPs (5-10-25. μg/mL) in human immortalized keratinocytes (HaCaT).After 24. h of treatment, CAPs were able to enter the cells leading to a decrease in viability, increased levels of 4-hydroxinonenal products (4-HNE) and IL-1α release. Overall these data, suggest lipid and protein oxidative damage, as well as an increase of inflammatory response after being challenged with CAPs. In addition, 3. h after CAPs exposure we found a significant increase in NF-kB and Nrf2 translocation into the nucleus. In contrast, no differences in gene expression and enzymatic activity of Nrf2 target genes were detected. This last finding could be explained by the ability of CAPs to possibly alter the binding of Nrf2 to the ARE DNA sequence.
publishDate 2017
dc.date.none.fl_str_mv 2017-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47421
Romani, Arianna; Cervellati, Carlo; Muresan, Ximena M.; Belmonte, Giuseppe; Pecorelli, Alessandra; et al.; Keratinocytes oxidative damage mechanisms related to airbone particle matter exposure; Elsevier Ireland; Mechanisms of Ageing and Development; 172; 11-2017; 86-95
0047-6374
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47421
identifier_str_mv Romani, Arianna; Cervellati, Carlo; Muresan, Ximena M.; Belmonte, Giuseppe; Pecorelli, Alessandra; et al.; Keratinocytes oxidative damage mechanisms related to airbone particle matter exposure; Elsevier Ireland; Mechanisms of Ageing and Development; 172; 11-2017; 86-95
0047-6374
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.mad.2017.11.007
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S0047637417300830
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Ireland
publisher.none.fl_str_mv Elsevier Ireland
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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