Decellularized ureteral scaffold (pig ureter) loaded with adipose mesenchymal stem cells (sheep adipose tissue) promotes ureter regeneration in a xenotransplant model (sheep ureter...

Autores
Fraunhoffer, Nicolas A; Meilerman, Analia; Ferraris, Sergio; Lange, Fernando; Casadei, Domingo; Guerrieri, Diego; Iovanna, Juan; Chuluyan, Hector Eduardo
Año de publicación
2020
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Ureteral injuries account for about 3% of urogenital traumas. Decellularized tissues have emerged as an alternative to ureteral repair, but the available protocols have failed in functional host integration. The aim of this study was to develop and validate in vivo a ureteral graft from a porcine ureteric scaffold, seeded with adipose mesenchymal stem cells (aMSCs). Ureteral samples from healthy pigs were used. Tissues were decellularized using Triton X-100 1% and SDS 0.1% under continuous intraluminal perfusion in a bioreactor designed by our group. Decellularization and structural integrity were characterized by histological analysis, β-actin western blot, residual DNA content, and scanning electron microscopy. Extracellular matrix (EMC) proteins and VEGF were studied by immunohistochemistry. Furthermore, 41 growth factors were analyzed by protein array. Recellularization was performed with aMSCs extracted from sheep adipose tissue, and it was evaluated histologically. Ureteral grafts were implanted into seven host sheep, and the functionality was analyzed by ureterography. At ten weeks, the implant was extracted, and integration was evaluated by histologically. Decellularized grafts showed high structural integrity and low DNA contamination and β-actin levels. EMC proteins and VEGF were observed. After cellularization with aMSC, the grafts showed the presence of groups of cells, and 32 growth factors were differentially detected. Sheep implants showed peristaltic movements and the regeneration of all ureteral tissue components. These results indicate that the protocol used is successful in achieving a decellularized ureter with an intact native architecture and recellularization with aMSCs. Also, the porcine ureteral scaffold seeded with aMSCs showed a high functional integration with the host tissue. Therefore, this type of graft may be a suitable alternative to ureteral regeneration.
Fil: Fraunhoffer, Nicolas A. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Meilerman, Analia. Inserm; Francia
Fil: Ferraris, Sergio. Universidad Maimónides; Argentina
Fil: Lange, Fernando. Universidad Maimónides; Argentina
Fil: Casadei, Domingo. Instituto de Transplante y alta Complejidad; Argentina
Fil: Guerrieri, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Iovanna, Juan. Inserm; Francia
Fil: Chuluyan, Hector Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
International Society for Stem Cell Research Annual Meeting
Estados Unidos
International Society for Stem Cell Research
Materia
URETER
BIOINGENIERIA
REGENERACION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/184973
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/184973
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Decellularized ureteral scaffold (pig ureter) loaded with adipose mesenchymal stem cells (sheep adipose tissue) promotes ureter regeneration in a xenotransplant model (sheep ureter)Fraunhoffer, Nicolas AMeilerman, AnaliaFerraris, SergioLange, FernandoCasadei, DomingoGuerrieri, DiegoIovanna, JuanChuluyan, Hector EduardoURETERBIOINGENIERIAREGENERACIONhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Ureteral injuries account for about 3% of urogenital traumas. Decellularized tissues have emerged as an alternative to ureteral repair, but the available protocols have failed in functional host integration. The aim of this study was to develop and validate in vivo a ureteral graft from a porcine ureteric scaffold, seeded with adipose mesenchymal stem cells (aMSCs). Ureteral samples from healthy pigs were used. Tissues were decellularized using Triton X-100 1% and SDS 0.1% under continuous intraluminal perfusion in a bioreactor designed by our group. Decellularization and structural integrity were characterized by histological analysis, β-actin western blot, residual DNA content, and scanning electron microscopy. Extracellular matrix (EMC) proteins and VEGF were studied by immunohistochemistry. Furthermore, 41 growth factors were analyzed by protein array. Recellularization was performed with aMSCs extracted from sheep adipose tissue, and it was evaluated histologically. Ureteral grafts were implanted into seven host sheep, and the functionality was analyzed by ureterography. At ten weeks, the implant was extracted, and integration was evaluated by histologically. Decellularized grafts showed high structural integrity and low DNA contamination and β-actin levels. EMC proteins and VEGF were observed. After cellularization with aMSC, the grafts showed the presence of groups of cells, and 32 growth factors were differentially detected. Sheep implants showed peristaltic movements and the regeneration of all ureteral tissue components. These results indicate that the protocol used is successful in achieving a decellularized ureter with an intact native architecture and recellularization with aMSCs. Also, the porcine ureteral scaffold seeded with aMSCs showed a high functional integration with the host tissue. Therefore, this type of graft may be a suitable alternative to ureteral regeneration.Fil: Fraunhoffer, Nicolas A. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Meilerman, Analia. Inserm; FranciaFil: Ferraris, Sergio. Universidad Maimónides; ArgentinaFil: Lange, Fernando. Universidad Maimónides; ArgentinaFil: Casadei, Domingo. Instituto de Transplante y alta Complejidad; ArgentinaFil: Guerrieri, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaFil: Iovanna, Juan. Inserm; FranciaFil: Chuluyan, Hector Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; ArgentinaInternational Society for Stem Cell Research Annual MeetingEstados UnidosInternational Society for Stem Cell ResearchInternactional Society for Stem Cell Research2020info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónBookhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/184973Decellularized ureteral scaffold (pig ureter) loaded with adipose mesenchymal stem cells (sheep adipose tissue) promotes ureter regeneration in a xenotransplant model (sheep ureter); International Society for Stem Cell Research Annual Meeting; Estados Unidos; 2020CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://static1.squarespace.com/static/611faaa8fee682525ee16489/t/62f54b950df1515922eeceac/1660242839740/ISSCR+2020+Poster+Abstract+Book.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:30:00Zoai:ri.conicet.gov.ar:11336/184973instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:30:01.164CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Decellularized ureteral scaffold (pig ureter) loaded with adipose mesenchymal stem cells (sheep adipose tissue) promotes ureter regeneration in a xenotransplant model (sheep ureter)
title Decellularized ureteral scaffold (pig ureter) loaded with adipose mesenchymal stem cells (sheep adipose tissue) promotes ureter regeneration in a xenotransplant model (sheep ureter)
spellingShingle Decellularized ureteral scaffold (pig ureter) loaded with adipose mesenchymal stem cells (sheep adipose tissue) promotes ureter regeneration in a xenotransplant model (sheep ureter)
Fraunhoffer, Nicolas A
URETER
BIOINGENIERIA
REGENERACION
title_short Decellularized ureteral scaffold (pig ureter) loaded with adipose mesenchymal stem cells (sheep adipose tissue) promotes ureter regeneration in a xenotransplant model (sheep ureter)
title_full Decellularized ureteral scaffold (pig ureter) loaded with adipose mesenchymal stem cells (sheep adipose tissue) promotes ureter regeneration in a xenotransplant model (sheep ureter)
title_fullStr Decellularized ureteral scaffold (pig ureter) loaded with adipose mesenchymal stem cells (sheep adipose tissue) promotes ureter regeneration in a xenotransplant model (sheep ureter)
title_full_unstemmed Decellularized ureteral scaffold (pig ureter) loaded with adipose mesenchymal stem cells (sheep adipose tissue) promotes ureter regeneration in a xenotransplant model (sheep ureter)
title_sort Decellularized ureteral scaffold (pig ureter) loaded with adipose mesenchymal stem cells (sheep adipose tissue) promotes ureter regeneration in a xenotransplant model (sheep ureter)
dc.creator.none.fl_str_mv Fraunhoffer, Nicolas A
Meilerman, Analia
Ferraris, Sergio
Lange, Fernando
Casadei, Domingo
Guerrieri, Diego
Iovanna, Juan
Chuluyan, Hector Eduardo
author Fraunhoffer, Nicolas A
author_facet Fraunhoffer, Nicolas A
Meilerman, Analia
Ferraris, Sergio
Lange, Fernando
Casadei, Domingo
Guerrieri, Diego
Iovanna, Juan
Chuluyan, Hector Eduardo
author_role author
author2 Meilerman, Analia
Ferraris, Sergio
Lange, Fernando
Casadei, Domingo
Guerrieri, Diego
Iovanna, Juan
Chuluyan, Hector Eduardo
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv URETER
BIOINGENIERIA
REGENERACION
topic URETER
BIOINGENIERIA
REGENERACION
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Ureteral injuries account for about 3% of urogenital traumas. Decellularized tissues have emerged as an alternative to ureteral repair, but the available protocols have failed in functional host integration. The aim of this study was to develop and validate in vivo a ureteral graft from a porcine ureteric scaffold, seeded with adipose mesenchymal stem cells (aMSCs). Ureteral samples from healthy pigs were used. Tissues were decellularized using Triton X-100 1% and SDS 0.1% under continuous intraluminal perfusion in a bioreactor designed by our group. Decellularization and structural integrity were characterized by histological analysis, β-actin western blot, residual DNA content, and scanning electron microscopy. Extracellular matrix (EMC) proteins and VEGF were studied by immunohistochemistry. Furthermore, 41 growth factors were analyzed by protein array. Recellularization was performed with aMSCs extracted from sheep adipose tissue, and it was evaluated histologically. Ureteral grafts were implanted into seven host sheep, and the functionality was analyzed by ureterography. At ten weeks, the implant was extracted, and integration was evaluated by histologically. Decellularized grafts showed high structural integrity and low DNA contamination and β-actin levels. EMC proteins and VEGF were observed. After cellularization with aMSC, the grafts showed the presence of groups of cells, and 32 growth factors were differentially detected. Sheep implants showed peristaltic movements and the regeneration of all ureteral tissue components. These results indicate that the protocol used is successful in achieving a decellularized ureter with an intact native architecture and recellularization with aMSCs. Also, the porcine ureteral scaffold seeded with aMSCs showed a high functional integration with the host tissue. Therefore, this type of graft may be a suitable alternative to ureteral regeneration.
Fil: Fraunhoffer, Nicolas A. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Meilerman, Analia. Inserm; Francia
Fil: Ferraris, Sergio. Universidad Maimónides; Argentina
Fil: Lange, Fernando. Universidad Maimónides; Argentina
Fil: Casadei, Domingo. Instituto de Transplante y alta Complejidad; Argentina
Fil: Guerrieri, Diego. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
Fil: Iovanna, Juan. Inserm; Francia
Fil: Chuluyan, Hector Eduardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Centro de Estudios Farmacológicos y Botánicos. Universidad de Buenos Aires. Facultad de Medicina. Centro de Estudios Farmacológicos y Botánicos; Argentina
International Society for Stem Cell Research Annual Meeting
Estados Unidos
International Society for Stem Cell Research
description Ureteral injuries account for about 3% of urogenital traumas. Decellularized tissues have emerged as an alternative to ureteral repair, but the available protocols have failed in functional host integration. The aim of this study was to develop and validate in vivo a ureteral graft from a porcine ureteric scaffold, seeded with adipose mesenchymal stem cells (aMSCs). Ureteral samples from healthy pigs were used. Tissues were decellularized using Triton X-100 1% and SDS 0.1% under continuous intraluminal perfusion in a bioreactor designed by our group. Decellularization and structural integrity were characterized by histological analysis, β-actin western blot, residual DNA content, and scanning electron microscopy. Extracellular matrix (EMC) proteins and VEGF were studied by immunohistochemistry. Furthermore, 41 growth factors were analyzed by protein array. Recellularization was performed with aMSCs extracted from sheep adipose tissue, and it was evaluated histologically. Ureteral grafts were implanted into seven host sheep, and the functionality was analyzed by ureterography. At ten weeks, the implant was extracted, and integration was evaluated by histologically. Decellularized grafts showed high structural integrity and low DNA contamination and β-actin levels. EMC proteins and VEGF were observed. After cellularization with aMSC, the grafts showed the presence of groups of cells, and 32 growth factors were differentially detected. Sheep implants showed peristaltic movements and the regeneration of all ureteral tissue components. These results indicate that the protocol used is successful in achieving a decellularized ureter with an intact native architecture and recellularization with aMSCs. Also, the porcine ureteral scaffold seeded with aMSCs showed a high functional integration with the host tissue. Therefore, this type of graft may be a suitable alternative to ureteral regeneration.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Book
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/184973
Decellularized ureteral scaffold (pig ureter) loaded with adipose mesenchymal stem cells (sheep adipose tissue) promotes ureter regeneration in a xenotransplant model (sheep ureter); International Society for Stem Cell Research Annual Meeting; Estados Unidos; 2020
CONICET Digital
CONICET
url http://hdl.handle.net/11336/184973
identifier_str_mv Decellularized ureteral scaffold (pig ureter) loaded with adipose mesenchymal stem cells (sheep adipose tissue) promotes ureter regeneration in a xenotransplant model (sheep ureter); International Society for Stem Cell Research Annual Meeting; Estados Unidos; 2020
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://static1.squarespace.com/static/611faaa8fee682525ee16489/t/62f54b950df1515922eeceac/1660242839740/ISSCR+2020+Poster+Abstract+Book.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Internactional Society for Stem Cell Research
publisher.none.fl_str_mv Internactional Society for Stem Cell Research
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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