Study of the Experimental Conditions and Mechanisms for Diclofenac Loading in Functionalized Magnetic Nanoparticles

Autores
Agotegaray, Mariela Alejandra; Lassalle, Verónica Leticia
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Magnetic nanoparticles (MNPs) composed of magnetite coated with oleic acid and functionalized with chitosan have been prepared to the targeted release of the non steroidal anti-inflammatory drug (NSAID) Diclofenac. Two procedures to incorporate the drug were explored: simple adsorption and covalent linkage. The impact of experimental variables such as the nature of the NSAID (commercial versus analytical sodium Diclofenac), its concentration and amount of coupling agent were evaluated in terms of the loading efficiency and the properties of interest of MNPs (size, surface functionality, stability). Loaded and unloaded MNPs were characterized by FTIR-DRIFTS spectroscopy, dynamic light scattering, zeta potential and transmission electron microscopy. UV-visible quantification of Diclofenac was achieved reaching values of loading efficiency between 11 and 27% depending on the experimental conditions. Probable mechanisms for drug-MNPs interactions have been proposed on the base of characterization data. A nanocarrier of size lower than 150 nm, with satisfactory loading skill and high dispersion capability on water was obtained. The release behavior demonstrated to be independent on the presence of an external magnetic field. The properties found in these nanosystems result relevant in view of their effective in vivo implementation.
Fil: Agotegaray, Mariela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Química del Sur; Argentina. Universidad Nacional del Sur; Argentina
Fil: Lassalle, Verónica Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Química del Sur; Argentina. Universidad Nacional del Sur; Argentina
Materia
MAGNETIC NANOPARTICLES
CHITOSAN
DICLOFENAC
DRUG TARGET
IN VITRO RELEASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/10367
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/10367
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Study of the Experimental Conditions and Mechanisms for Diclofenac Loading in Functionalized Magnetic NanoparticlesAgotegaray, Mariela AlejandraLassalle, Verónica LeticiaMAGNETIC NANOPARTICLESCHITOSANDICLOFENACDRUG TARGETIN VITRO RELEASEhttps://purl.org/becyt/ford/2.10https://purl.org/becyt/ford/2Magnetic nanoparticles (MNPs) composed of magnetite coated with oleic acid and functionalized with chitosan have been prepared to the targeted release of the non steroidal anti-inflammatory drug (NSAID) Diclofenac. Two procedures to incorporate the drug were explored: simple adsorption and covalent linkage. The impact of experimental variables such as the nature of the NSAID (commercial versus analytical sodium Diclofenac), its concentration and amount of coupling agent were evaluated in terms of the loading efficiency and the properties of interest of MNPs (size, surface functionality, stability). Loaded and unloaded MNPs were characterized by FTIR-DRIFTS spectroscopy, dynamic light scattering, zeta potential and transmission electron microscopy. UV-visible quantification of Diclofenac was achieved reaching values of loading efficiency between 11 and 27% depending on the experimental conditions. Probable mechanisms for drug-MNPs interactions have been proposed on the base of characterization data. A nanocarrier of size lower than 150 nm, with satisfactory loading skill and high dispersion capability on water was obtained. The release behavior demonstrated to be independent on the presence of an external magnetic field. The properties found in these nanosystems result relevant in view of their effective in vivo implementation.Fil: Agotegaray, Mariela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Química del Sur; Argentina. Universidad Nacional del Sur; ArgentinaFil: Lassalle, Verónica Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Química del Sur; Argentina. Universidad Nacional del Sur; ArgentinaAarya Publishing House2014-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/10367Agotegaray, Mariela Alejandra; Lassalle, Verónica Leticia; Study of the Experimental Conditions and Mechanisms for Diclofenac Loading in Functionalized Magnetic Nanoparticles; Aarya Publishing House; International Journal of Chemical and Pharmaceutical Analysis; 1; 4; 7-2014; 154-1642395 - 24662348-0726enginfo:eu-repo/semantics/altIdentifier/url/http://pmindexing.com/journals/index.php/IJCPA/article/view/288info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:05:16Zoai:ri.conicet.gov.ar:11336/10367instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:05:16.827CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Study of the Experimental Conditions and Mechanisms for Diclofenac Loading in Functionalized Magnetic Nanoparticles
title Study of the Experimental Conditions and Mechanisms for Diclofenac Loading in Functionalized Magnetic Nanoparticles
spellingShingle Study of the Experimental Conditions and Mechanisms for Diclofenac Loading in Functionalized Magnetic Nanoparticles
Agotegaray, Mariela Alejandra
MAGNETIC NANOPARTICLES
CHITOSAN
DICLOFENAC
DRUG TARGET
IN VITRO RELEASE
title_short Study of the Experimental Conditions and Mechanisms for Diclofenac Loading in Functionalized Magnetic Nanoparticles
title_full Study of the Experimental Conditions and Mechanisms for Diclofenac Loading in Functionalized Magnetic Nanoparticles
title_fullStr Study of the Experimental Conditions and Mechanisms for Diclofenac Loading in Functionalized Magnetic Nanoparticles
title_full_unstemmed Study of the Experimental Conditions and Mechanisms for Diclofenac Loading in Functionalized Magnetic Nanoparticles
title_sort Study of the Experimental Conditions and Mechanisms for Diclofenac Loading in Functionalized Magnetic Nanoparticles
dc.creator.none.fl_str_mv Agotegaray, Mariela Alejandra
Lassalle, Verónica Leticia
author Agotegaray, Mariela Alejandra
author_facet Agotegaray, Mariela Alejandra
Lassalle, Verónica Leticia
author_role author
author2 Lassalle, Verónica Leticia
author2_role author
dc.subject.none.fl_str_mv MAGNETIC NANOPARTICLES
CHITOSAN
DICLOFENAC
DRUG TARGET
IN VITRO RELEASE
topic MAGNETIC NANOPARTICLES
CHITOSAN
DICLOFENAC
DRUG TARGET
IN VITRO RELEASE
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.10
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Magnetic nanoparticles (MNPs) composed of magnetite coated with oleic acid and functionalized with chitosan have been prepared to the targeted release of the non steroidal anti-inflammatory drug (NSAID) Diclofenac. Two procedures to incorporate the drug were explored: simple adsorption and covalent linkage. The impact of experimental variables such as the nature of the NSAID (commercial versus analytical sodium Diclofenac), its concentration and amount of coupling agent were evaluated in terms of the loading efficiency and the properties of interest of MNPs (size, surface functionality, stability). Loaded and unloaded MNPs were characterized by FTIR-DRIFTS spectroscopy, dynamic light scattering, zeta potential and transmission electron microscopy. UV-visible quantification of Diclofenac was achieved reaching values of loading efficiency between 11 and 27% depending on the experimental conditions. Probable mechanisms for drug-MNPs interactions have been proposed on the base of characterization data. A nanocarrier of size lower than 150 nm, with satisfactory loading skill and high dispersion capability on water was obtained. The release behavior demonstrated to be independent on the presence of an external magnetic field. The properties found in these nanosystems result relevant in view of their effective in vivo implementation.
Fil: Agotegaray, Mariela Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Química del Sur; Argentina. Universidad Nacional del Sur; Argentina
Fil: Lassalle, Verónica Leticia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Bahía Blanca. Instituto de Química del Sur; Argentina. Universidad Nacional del Sur; Argentina
description Magnetic nanoparticles (MNPs) composed of magnetite coated with oleic acid and functionalized with chitosan have been prepared to the targeted release of the non steroidal anti-inflammatory drug (NSAID) Diclofenac. Two procedures to incorporate the drug were explored: simple adsorption and covalent linkage. The impact of experimental variables such as the nature of the NSAID (commercial versus analytical sodium Diclofenac), its concentration and amount of coupling agent were evaluated in terms of the loading efficiency and the properties of interest of MNPs (size, surface functionality, stability). Loaded and unloaded MNPs were characterized by FTIR-DRIFTS spectroscopy, dynamic light scattering, zeta potential and transmission electron microscopy. UV-visible quantification of Diclofenac was achieved reaching values of loading efficiency between 11 and 27% depending on the experimental conditions. Probable mechanisms for drug-MNPs interactions have been proposed on the base of characterization data. A nanocarrier of size lower than 150 nm, with satisfactory loading skill and high dispersion capability on water was obtained. The release behavior demonstrated to be independent on the presence of an external magnetic field. The properties found in these nanosystems result relevant in view of their effective in vivo implementation.
publishDate 2014
dc.date.none.fl_str_mv 2014-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/10367
Agotegaray, Mariela Alejandra; Lassalle, Verónica Leticia; Study of the Experimental Conditions and Mechanisms for Diclofenac Loading in Functionalized Magnetic Nanoparticles; Aarya Publishing House; International Journal of Chemical and Pharmaceutical Analysis; 1; 4; 7-2014; 154-164
2395 - 2466
2348-0726
url http://hdl.handle.net/11336/10367
identifier_str_mv Agotegaray, Mariela Alejandra; Lassalle, Verónica Leticia; Study of the Experimental Conditions and Mechanisms for Diclofenac Loading in Functionalized Magnetic Nanoparticles; Aarya Publishing House; International Journal of Chemical and Pharmaceutical Analysis; 1; 4; 7-2014; 154-164
2395 - 2466
2348-0726
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://pmindexing.com/journals/index.php/IJCPA/article/view/288
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Aarya Publishing House
publisher.none.fl_str_mv Aarya Publishing House
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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