Serotype distribution of plasmid-encoded virulence profiles, and identification of espP and subAB alleles in verotoxigenic escherichia coli

Autores
Sanso, Andrea Mariel; Bustamante, Ana Victoria; Franci, Tomas; González, Juliana; Cadona, Jimena Soledad; Lucchesi, Paula Maria Alejandra
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aims: This study was designed to characterize verotoxigenic Escherichia coli (VTEC), important food-borne pathogens, in relation to virulence genes found in large plasmids harboured by diseaseassociated strains. Our aim was to detect these genes and possible combinations of them, and to establish if some kind of relationship exists between these profiles and different serotypes. Study Design: Amplification of genes and allelic variants by PCR. Place and Duration of Study: Laboratorio de Inmunoquímica y Biotecnología (FCV-UNCPBA, Argentina), between June 2010 and July 2013. Methodology: 208 VTEC isolates belonging to 49 serotypes were characterized for the presence of plasmid-encoded genes: epeA (serine-protease), espP (extracellular serine protease) and its variants, katP (periplasmic catalase-peroxidase), stcE (zinc metalloprotease) and subA (subtilase cytotoxin) and its variants. Results: The most frequently detected gene was espP (87%), followed by ehxA (47%), saa (20%) and katP (17%), whereas epeA, subA and stcE ranged from 7 to 12%. Taking into account these genes, twelve profiles were observed, ranging from the presence of zero to five of the genes. Some serotypes presented only one plasmid profile whereas others, such as O20:H19, showed up to four different profiles. Functional differences have been previously found among EspP subtypes, and we found that several VTEC isolated from bovines or foods contained the espPα, which is one of the alleles possibly associated with severe human disease. Considering subtilase cytotoxin gene subtypes, only subAB1 was identified. Conclusion: Our results show that VTEC strains can possess different combinations of plasmidencoded virulence genes and even strains belonging to the same serotype can differ in relation to their plasmid genetic composition. In relation to allelic variants, LEE-positive serotypes showed for espP gene the allele α or the γ, and the detected subAB allele differed from the prevalent allelic variant subAB2 carried by strains circulating in sheeps and humans in European countries.
Fil: Sanso, Andrea Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Bustamante, Ana Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Franci, Tomas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: González, Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Cadona, Jimena Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Lucchesi, Paula Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Materia
PLASMID PROFILES
VEROTOXIGENIC ESCHERICHIA COLI
VIRULENCE
ESPP ALLELES
SUBAB ALLELES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/27947
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/27947
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Serotype distribution of plasmid-encoded virulence profiles, and identification of espP and subAB alleles in verotoxigenic escherichia coliSanso, Andrea MarielBustamante, Ana VictoriaFranci, TomasGonzález, JulianaCadona, Jimena SoledadLucchesi, Paula Maria AlejandraPLASMID PROFILESVEROTOXIGENIC ESCHERICHIA COLIVIRULENCEESPP ALLELESSUBAB ALLELEShttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Aims: This study was designed to characterize verotoxigenic Escherichia coli (VTEC), important food-borne pathogens, in relation to virulence genes found in large plasmids harboured by diseaseassociated strains. Our aim was to detect these genes and possible combinations of them, and to establish if some kind of relationship exists between these profiles and different serotypes. Study Design: Amplification of genes and allelic variants by PCR. Place and Duration of Study: Laboratorio de Inmunoquímica y Biotecnología (FCV-UNCPBA, Argentina), between June 2010 and July 2013. Methodology: 208 VTEC isolates belonging to 49 serotypes were characterized for the presence of plasmid-encoded genes: epeA (serine-protease), espP (extracellular serine protease) and its variants, katP (periplasmic catalase-peroxidase), stcE (zinc metalloprotease) and subA (subtilase cytotoxin) and its variants. Results: The most frequently detected gene was espP (87%), followed by ehxA (47%), saa (20%) and katP (17%), whereas epeA, subA and stcE ranged from 7 to 12%. Taking into account these genes, twelve profiles were observed, ranging from the presence of zero to five of the genes. Some serotypes presented only one plasmid profile whereas others, such as O20:H19, showed up to four different profiles. Functional differences have been previously found among EspP subtypes, and we found that several VTEC isolated from bovines or foods contained the espPα, which is one of the alleles possibly associated with severe human disease. Considering subtilase cytotoxin gene subtypes, only subAB1 was identified. Conclusion: Our results show that VTEC strains can possess different combinations of plasmidencoded virulence genes and even strains belonging to the same serotype can differ in relation to their plasmid genetic composition. In relation to allelic variants, LEE-positive serotypes showed for espP gene the allele α or the γ, and the detected subAB allele differed from the prevalent allelic variant subAB2 carried by strains circulating in sheeps and humans in European countries.Fil: Sanso, Andrea Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; ArgentinaFil: Bustamante, Ana Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; ArgentinaFil: Franci, Tomas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; ArgentinaFil: González, Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; ArgentinaFil: Cadona, Jimena Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; ArgentinaFil: Lucchesi, Paula Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; ArgentinaScience Domain International2014-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/27947Sanso, Andrea Mariel; Bustamante, Ana Victoria; Franci, Tomas; González, Juliana; Cadona, Jimena Soledad; et al.; Serotype distribution of plasmid-encoded virulence profiles, and identification of espP and subAB alleles in verotoxigenic escherichia coli; Science Domain International; British Microbiology Research Journal; 5; 5; 11-2014; 396-4042231-0886CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.9734/BMRJ/2015/13905info:eu-repo/semantics/openAccessAtribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)https://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:53:35Zoai:ri.conicet.gov.ar:11336/27947instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:53:35.415CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Serotype distribution of plasmid-encoded virulence profiles, and identification of espP and subAB alleles in verotoxigenic escherichia coli
title Serotype distribution of plasmid-encoded virulence profiles, and identification of espP and subAB alleles in verotoxigenic escherichia coli
spellingShingle Serotype distribution of plasmid-encoded virulence profiles, and identification of espP and subAB alleles in verotoxigenic escherichia coli
Sanso, Andrea Mariel
PLASMID PROFILES
VEROTOXIGENIC ESCHERICHIA COLI
VIRULENCE
ESPP ALLELES
SUBAB ALLELES
title_short Serotype distribution of plasmid-encoded virulence profiles, and identification of espP and subAB alleles in verotoxigenic escherichia coli
title_full Serotype distribution of plasmid-encoded virulence profiles, and identification of espP and subAB alleles in verotoxigenic escherichia coli
title_fullStr Serotype distribution of plasmid-encoded virulence profiles, and identification of espP and subAB alleles in verotoxigenic escherichia coli
title_full_unstemmed Serotype distribution of plasmid-encoded virulence profiles, and identification of espP and subAB alleles in verotoxigenic escherichia coli
title_sort Serotype distribution of plasmid-encoded virulence profiles, and identification of espP and subAB alleles in verotoxigenic escherichia coli
dc.creator.none.fl_str_mv Sanso, Andrea Mariel
Bustamante, Ana Victoria
Franci, Tomas
González, Juliana
Cadona, Jimena Soledad
Lucchesi, Paula Maria Alejandra
author Sanso, Andrea Mariel
author_facet Sanso, Andrea Mariel
Bustamante, Ana Victoria
Franci, Tomas
González, Juliana
Cadona, Jimena Soledad
Lucchesi, Paula Maria Alejandra
author_role author
author2 Bustamante, Ana Victoria
Franci, Tomas
González, Juliana
Cadona, Jimena Soledad
Lucchesi, Paula Maria Alejandra
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv PLASMID PROFILES
VEROTOXIGENIC ESCHERICHIA COLI
VIRULENCE
ESPP ALLELES
SUBAB ALLELES
topic PLASMID PROFILES
VEROTOXIGENIC ESCHERICHIA COLI
VIRULENCE
ESPP ALLELES
SUBAB ALLELES
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Aims: This study was designed to characterize verotoxigenic Escherichia coli (VTEC), important food-borne pathogens, in relation to virulence genes found in large plasmids harboured by diseaseassociated strains. Our aim was to detect these genes and possible combinations of them, and to establish if some kind of relationship exists between these profiles and different serotypes. Study Design: Amplification of genes and allelic variants by PCR. Place and Duration of Study: Laboratorio de Inmunoquímica y Biotecnología (FCV-UNCPBA, Argentina), between June 2010 and July 2013. Methodology: 208 VTEC isolates belonging to 49 serotypes were characterized for the presence of plasmid-encoded genes: epeA (serine-protease), espP (extracellular serine protease) and its variants, katP (periplasmic catalase-peroxidase), stcE (zinc metalloprotease) and subA (subtilase cytotoxin) and its variants. Results: The most frequently detected gene was espP (87%), followed by ehxA (47%), saa (20%) and katP (17%), whereas epeA, subA and stcE ranged from 7 to 12%. Taking into account these genes, twelve profiles were observed, ranging from the presence of zero to five of the genes. Some serotypes presented only one plasmid profile whereas others, such as O20:H19, showed up to four different profiles. Functional differences have been previously found among EspP subtypes, and we found that several VTEC isolated from bovines or foods contained the espPα, which is one of the alleles possibly associated with severe human disease. Considering subtilase cytotoxin gene subtypes, only subAB1 was identified. Conclusion: Our results show that VTEC strains can possess different combinations of plasmidencoded virulence genes and even strains belonging to the same serotype can differ in relation to their plasmid genetic composition. In relation to allelic variants, LEE-positive serotypes showed for espP gene the allele α or the γ, and the detected subAB allele differed from the prevalent allelic variant subAB2 carried by strains circulating in sheeps and humans in European countries.
Fil: Sanso, Andrea Mariel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Bustamante, Ana Victoria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Franci, Tomas. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: González, Juliana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Cadona, Jimena Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
Fil: Lucchesi, Paula Maria Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Tandil. Centro de Investigacion Veterinaria de Tandil; Argentina
description Aims: This study was designed to characterize verotoxigenic Escherichia coli (VTEC), important food-borne pathogens, in relation to virulence genes found in large plasmids harboured by diseaseassociated strains. Our aim was to detect these genes and possible combinations of them, and to establish if some kind of relationship exists between these profiles and different serotypes. Study Design: Amplification of genes and allelic variants by PCR. Place and Duration of Study: Laboratorio de Inmunoquímica y Biotecnología (FCV-UNCPBA, Argentina), between June 2010 and July 2013. Methodology: 208 VTEC isolates belonging to 49 serotypes were characterized for the presence of plasmid-encoded genes: epeA (serine-protease), espP (extracellular serine protease) and its variants, katP (periplasmic catalase-peroxidase), stcE (zinc metalloprotease) and subA (subtilase cytotoxin) and its variants. Results: The most frequently detected gene was espP (87%), followed by ehxA (47%), saa (20%) and katP (17%), whereas epeA, subA and stcE ranged from 7 to 12%. Taking into account these genes, twelve profiles were observed, ranging from the presence of zero to five of the genes. Some serotypes presented only one plasmid profile whereas others, such as O20:H19, showed up to four different profiles. Functional differences have been previously found among EspP subtypes, and we found that several VTEC isolated from bovines or foods contained the espPα, which is one of the alleles possibly associated with severe human disease. Considering subtilase cytotoxin gene subtypes, only subAB1 was identified. Conclusion: Our results show that VTEC strains can possess different combinations of plasmidencoded virulence genes and even strains belonging to the same serotype can differ in relation to their plasmid genetic composition. In relation to allelic variants, LEE-positive serotypes showed for espP gene the allele α or the γ, and the detected subAB allele differed from the prevalent allelic variant subAB2 carried by strains circulating in sheeps and humans in European countries.
publishDate 2014
dc.date.none.fl_str_mv 2014-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/27947
Sanso, Andrea Mariel; Bustamante, Ana Victoria; Franci, Tomas; González, Juliana; Cadona, Jimena Soledad; et al.; Serotype distribution of plasmid-encoded virulence profiles, and identification of espP and subAB alleles in verotoxigenic escherichia coli; Science Domain International; British Microbiology Research Journal; 5; 5; 11-2014; 396-404
2231-0886
CONICET Digital
CONICET
url http://hdl.handle.net/11336/27947
identifier_str_mv Sanso, Andrea Mariel; Bustamante, Ana Victoria; Franci, Tomas; González, Juliana; Cadona, Jimena Soledad; et al.; Serotype distribution of plasmid-encoded virulence profiles, and identification of espP and subAB alleles in verotoxigenic escherichia coli; Science Domain International; British Microbiology Research Journal; 5; 5; 11-2014; 396-404
2231-0886
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.9734/BMRJ/2015/13905
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv Atribución-NoComercial-CompartirIgual 2.5 Argentina (CC BY-NC-SA 2.5 AR)
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Science Domain International
publisher.none.fl_str_mv Science Domain International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846083067023720448
score 13.22299

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