Role and clinical course of verotoxigenic Escherichia coli infections in childhood acute diarrhoea in Argentina

Autores
Rivero, Mariana Alejandra; Passucci, Juan Antonio; Rodriguez, Edgardo Mario; Parma, Alberto Ernesto
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of the study was to investigate the role and clinical course of verotoxigenic Escherichia coli (VTEC) infections in children with acute diarrhoea from Argentina, the country with the highest worldwide incidence of haemolytic uraemic syndrome (HUS). To accomplish our objective, 437 samples from children up to 6 years old with acute diarrhoea were collected and processed. More than 60% of the children studied presented watery or mucous diarrhoea without blood, and in 25.2% of the cases the samples were with blood. In a first screening, a multiplex PCR was performed to detect the presence of vt1, vt2, eae, ehxA, saa virulence genes Then, the strains were isolated and analyzed in order to characterize their serotypes, virulence genes, antibiotic susceptibility profiles and verotoxin (VT) production. Forty four of the 437 samples (10.06%) were positive to VTEC virulence genes. VTEC-infected patients presented different types of diarrhoea (27.27% belonged to non-bloody type). Several serotypes and virulence genotypes were found. Isolates belonged to the serotypes O157:H7, O145:H-, O26:H11, O121:H19, O111:H2, and O118:H2. HUS developed in 16 (36.4%) patients corresponding to positive samples. All the VTEC isolates produced a cytopathic effect on Vero cells monolayer, confirming the ability for VT expression. Despite most strains were sensitive to all antimicrobials studied a positive association between clinical progression to HUS and antibiotic therapy, both for the total of patients studied, as well as for the VTEC-positive group was observed. In conclusion, the data obtained in this study increase the knowledge on the role and clinical course of VTEC infection in childhood acute diarrhoea beyond bloody diarrhea, and might be considered for the prevention, diagnosis and management of this disease. It is possible that the optimal approach for VTEC diagnosis could be through searching by multiplex PCR for the presence of vt1, vt2, eae and ehxA genes.
Fil: Rivero, Mariana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; Argentina
Fil: Passucci, Juan Antonio. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; Argentina
Fil: Rodriguez, Edgardo Mario. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; Argentina
Fil: Parma, Alberto Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; Argentina
Materia
VEROTOXIGENIC
E COLI
CLINICAL COURSE
ARGENTINA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/242002
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/242002
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Role and clinical course of verotoxigenic Escherichia coli infections in childhood acute diarrhoea in ArgentinaRivero, Mariana AlejandraPassucci, Juan AntonioRodriguez, Edgardo MarioParma, Alberto ErnestoVEROTOXIGENICE COLICLINICAL COURSEARGENTINAhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The aim of the study was to investigate the role and clinical course of verotoxigenic Escherichia coli (VTEC) infections in children with acute diarrhoea from Argentina, the country with the highest worldwide incidence of haemolytic uraemic syndrome (HUS). To accomplish our objective, 437 samples from children up to 6 years old with acute diarrhoea were collected and processed. More than 60% of the children studied presented watery or mucous diarrhoea without blood, and in 25.2% of the cases the samples were with blood. In a first screening, a multiplex PCR was performed to detect the presence of vt1, vt2, eae, ehxA, saa virulence genes Then, the strains were isolated and analyzed in order to characterize their serotypes, virulence genes, antibiotic susceptibility profiles and verotoxin (VT) production. Forty four of the 437 samples (10.06%) were positive to VTEC virulence genes. VTEC-infected patients presented different types of diarrhoea (27.27% belonged to non-bloody type). Several serotypes and virulence genotypes were found. Isolates belonged to the serotypes O157:H7, O145:H-, O26:H11, O121:H19, O111:H2, and O118:H2. HUS developed in 16 (36.4%) patients corresponding to positive samples. All the VTEC isolates produced a cytopathic effect on Vero cells monolayer, confirming the ability for VT expression. Despite most strains were sensitive to all antimicrobials studied a positive association between clinical progression to HUS and antibiotic therapy, both for the total of patients studied, as well as for the VTEC-positive group was observed. In conclusion, the data obtained in this study increase the knowledge on the role and clinical course of VTEC infection in childhood acute diarrhoea beyond bloody diarrhea, and might be considered for the prevention, diagnosis and management of this disease. It is possible that the optimal approach for VTEC diagnosis could be through searching by multiplex PCR for the presence of vt1, vt2, eae and ehxA genes.Fil: Rivero, Mariana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; ArgentinaFil: Passucci, Juan Antonio. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; ArgentinaFil: Rodriguez, Edgardo Mario. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; ArgentinaFil: Parma, Alberto Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; ArgentinaSociety for General Microbiology2010-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/242002Rivero, Mariana Alejandra; Passucci, Juan Antonio; Rodriguez, Edgardo Mario; Parma, Alberto Ernesto; Role and clinical course of verotoxigenic Escherichia coli infections in childhood acute diarrhoea in Argentina; Society for General Microbiology; Journal of Medical Microbiology; 59; 3; 3-2010; 345-3520022-2615CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.015560-0info:eu-repo/semantics/altIdentifier/doi/10.1099/jmm.0.015560-0info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:14:36Zoai:ri.conicet.gov.ar:11336/242002instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:14:36.815CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Role and clinical course of verotoxigenic Escherichia coli infections in childhood acute diarrhoea in Argentina
title Role and clinical course of verotoxigenic Escherichia coli infections in childhood acute diarrhoea in Argentina
spellingShingle Role and clinical course of verotoxigenic Escherichia coli infections in childhood acute diarrhoea in Argentina
Rivero, Mariana Alejandra
VEROTOXIGENIC
E COLI
CLINICAL COURSE
ARGENTINA
title_short Role and clinical course of verotoxigenic Escherichia coli infections in childhood acute diarrhoea in Argentina
title_full Role and clinical course of verotoxigenic Escherichia coli infections in childhood acute diarrhoea in Argentina
title_fullStr Role and clinical course of verotoxigenic Escherichia coli infections in childhood acute diarrhoea in Argentina
title_full_unstemmed Role and clinical course of verotoxigenic Escherichia coli infections in childhood acute diarrhoea in Argentina
title_sort Role and clinical course of verotoxigenic Escherichia coli infections in childhood acute diarrhoea in Argentina
dc.creator.none.fl_str_mv Rivero, Mariana Alejandra
Passucci, Juan Antonio
Rodriguez, Edgardo Mario
Parma, Alberto Ernesto
author Rivero, Mariana Alejandra
author_facet Rivero, Mariana Alejandra
Passucci, Juan Antonio
Rodriguez, Edgardo Mario
Parma, Alberto Ernesto
author_role author
author2 Passucci, Juan Antonio
Rodriguez, Edgardo Mario
Parma, Alberto Ernesto
author2_role author
author
author
dc.subject.none.fl_str_mv VEROTOXIGENIC
E COLI
CLINICAL COURSE
ARGENTINA
topic VEROTOXIGENIC
E COLI
CLINICAL COURSE
ARGENTINA
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The aim of the study was to investigate the role and clinical course of verotoxigenic Escherichia coli (VTEC) infections in children with acute diarrhoea from Argentina, the country with the highest worldwide incidence of haemolytic uraemic syndrome (HUS). To accomplish our objective, 437 samples from children up to 6 years old with acute diarrhoea were collected and processed. More than 60% of the children studied presented watery or mucous diarrhoea without blood, and in 25.2% of the cases the samples were with blood. In a first screening, a multiplex PCR was performed to detect the presence of vt1, vt2, eae, ehxA, saa virulence genes Then, the strains were isolated and analyzed in order to characterize their serotypes, virulence genes, antibiotic susceptibility profiles and verotoxin (VT) production. Forty four of the 437 samples (10.06%) were positive to VTEC virulence genes. VTEC-infected patients presented different types of diarrhoea (27.27% belonged to non-bloody type). Several serotypes and virulence genotypes were found. Isolates belonged to the serotypes O157:H7, O145:H-, O26:H11, O121:H19, O111:H2, and O118:H2. HUS developed in 16 (36.4%) patients corresponding to positive samples. All the VTEC isolates produced a cytopathic effect on Vero cells monolayer, confirming the ability for VT expression. Despite most strains were sensitive to all antimicrobials studied a positive association between clinical progression to HUS and antibiotic therapy, both for the total of patients studied, as well as for the VTEC-positive group was observed. In conclusion, the data obtained in this study increase the knowledge on the role and clinical course of VTEC infection in childhood acute diarrhoea beyond bloody diarrhea, and might be considered for the prevention, diagnosis and management of this disease. It is possible that the optimal approach for VTEC diagnosis could be through searching by multiplex PCR for the presence of vt1, vt2, eae and ehxA genes.
Fil: Rivero, Mariana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; Argentina
Fil: Passucci, Juan Antonio. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; Argentina
Fil: Rodriguez, Edgardo Mario. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; Argentina
Fil: Parma, Alberto Ernesto. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil; Argentina. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva; Argentina
description The aim of the study was to investigate the role and clinical course of verotoxigenic Escherichia coli (VTEC) infections in children with acute diarrhoea from Argentina, the country with the highest worldwide incidence of haemolytic uraemic syndrome (HUS). To accomplish our objective, 437 samples from children up to 6 years old with acute diarrhoea were collected and processed. More than 60% of the children studied presented watery or mucous diarrhoea without blood, and in 25.2% of the cases the samples were with blood. In a first screening, a multiplex PCR was performed to detect the presence of vt1, vt2, eae, ehxA, saa virulence genes Then, the strains were isolated and analyzed in order to characterize their serotypes, virulence genes, antibiotic susceptibility profiles and verotoxin (VT) production. Forty four of the 437 samples (10.06%) were positive to VTEC virulence genes. VTEC-infected patients presented different types of diarrhoea (27.27% belonged to non-bloody type). Several serotypes and virulence genotypes were found. Isolates belonged to the serotypes O157:H7, O145:H-, O26:H11, O121:H19, O111:H2, and O118:H2. HUS developed in 16 (36.4%) patients corresponding to positive samples. All the VTEC isolates produced a cytopathic effect on Vero cells monolayer, confirming the ability for VT expression. Despite most strains were sensitive to all antimicrobials studied a positive association between clinical progression to HUS and antibiotic therapy, both for the total of patients studied, as well as for the VTEC-positive group was observed. In conclusion, the data obtained in this study increase the knowledge on the role and clinical course of VTEC infection in childhood acute diarrhoea beyond bloody diarrhea, and might be considered for the prevention, diagnosis and management of this disease. It is possible that the optimal approach for VTEC diagnosis could be through searching by multiplex PCR for the presence of vt1, vt2, eae and ehxA genes.
publishDate 2010
dc.date.none.fl_str_mv 2010-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/242002
Rivero, Mariana Alejandra; Passucci, Juan Antonio; Rodriguez, Edgardo Mario; Parma, Alberto Ernesto; Role and clinical course of verotoxigenic Escherichia coli infections in childhood acute diarrhoea in Argentina; Society for General Microbiology; Journal of Medical Microbiology; 59; 3; 3-2010; 345-352
0022-2615
CONICET Digital
CONICET
url http://hdl.handle.net/11336/242002
identifier_str_mv Rivero, Mariana Alejandra; Passucci, Juan Antonio; Rodriguez, Edgardo Mario; Parma, Alberto Ernesto; Role and clinical course of verotoxigenic Escherichia coli infections in childhood acute diarrhoea in Argentina; Society for General Microbiology; Journal of Medical Microbiology; 59; 3; 3-2010; 345-352
0022-2615
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.015560-0
info:eu-repo/semantics/altIdentifier/doi/10.1099/jmm.0.015560-0
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Society for General Microbiology
publisher.none.fl_str_mv Society for General Microbiology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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