Identification of Anaplasma marginale, Babesia bovis and Babesia bigemina resistance alleles in Crioulo Lageano cattle using PCR-SBT and BoLA-DRB3 gene sequencing

Autores
da Silva Casa, Mariana; Giovambattista, Guillermo; Fonteque, Graziela Vieira; Miguel, Ellen Lara; Vogel, Carla Ivane Ganz; Miletti, Luiz Claudio; Takeshima, Shin Nosuke; Fonteque, Joandes Henrique
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Introduction: The BoLA-DRB3 gene in cattle is associated with tolerance to several infectious diseases, such as neosporosis, dermatophilosis, leukosis, and mastitis. Methods: This study used PCR-SBT and BoLA-DRB3 gene sequencing to determine the association between the presence or absence of Anaplasma marginale, Babesia bovis, and Babesia bigemina infections in 208 Crioulo Lageano cattle and alleles present in the population. The chi-square test and odds ratio analysis were employed to establish the association. Results: Of the BoLA-DRB3 gene alleles present in the population, two alleles were significantly associated with resistance to A. marginale infections: BoLA-DRB3001:01 (p < 0.001; OR = 0.224), which had a frequency of 7.93%, and BoLA-DRB3024:06 (p = 0.007; OR < 0.00001), which had a frequency of 0.72%. Regarding B. bovis infection, the BoLA-DRB3*011:01 allele (p = 0.002; OR = 0.271) had a frequency of 6% in the population and was associated with resistance to the infection. None of the alleles was associated with resistance to infection by B. bigemina. Discussion: The Crioulo Lageano breed has alleles that may confer resistance against infection by A. marginale and B. bovis.
Fil: da Silva Casa, Mariana. Universidade Federal de Santa Catarina; Brasil
Fil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Fonteque, Graziela Vieira. Universidade Federal de Santa Catarina; Brasil
Fil: Miguel, Ellen Lara. Universidade Federal de Santa Catarina; Brasil
Fil: Vogel, Carla Ivane Ganz. Universidade Federal de Santa Catarina; Brasil
Fil: Miletti, Luiz Claudio. Universidade Federal de Santa Catarina; Brasil
Fil: Takeshima, Shin Nosuke. Jumonji University; Japón
Fil: Fonteque, Joandes Henrique. Universidade Federal de Santa Catarina; Brasil
Materia
ALLELE
ANAPLASMA
BABESIA
CREOLE BREED
PCR
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/253694
Acceder
id CONICETDig_20497a1546f0923eeea26e155e1dc422
oai_identifier_str oai:ri.conicet.gov.ar:11336/253694
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Identification of Anaplasma marginale, Babesia bovis and Babesia bigemina resistance alleles in Crioulo Lageano cattle using PCR-SBT and BoLA-DRB3 gene sequencingda Silva Casa, MarianaGiovambattista, GuillermoFonteque, Graziela VieiraMiguel, Ellen LaraVogel, Carla Ivane GanzMiletti, Luiz ClaudioTakeshima, Shin NosukeFonteque, Joandes HenriqueALLELEANAPLASMABABESIACREOLE BREEDPCRhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Introduction: The BoLA-DRB3 gene in cattle is associated with tolerance to several infectious diseases, such as neosporosis, dermatophilosis, leukosis, and mastitis. Methods: This study used PCR-SBT and BoLA-DRB3 gene sequencing to determine the association between the presence or absence of Anaplasma marginale, Babesia bovis, and Babesia bigemina infections in 208 Crioulo Lageano cattle and alleles present in the population. The chi-square test and odds ratio analysis were employed to establish the association. Results: Of the BoLA-DRB3 gene alleles present in the population, two alleles were significantly associated with resistance to A. marginale infections: BoLA-DRB3001:01 (p < 0.001; OR = 0.224), which had a frequency of 7.93%, and BoLA-DRB3024:06 (p = 0.007; OR < 0.00001), which had a frequency of 0.72%. Regarding B. bovis infection, the BoLA-DRB3*011:01 allele (p = 0.002; OR = 0.271) had a frequency of 6% in the population and was associated with resistance to the infection. None of the alleles was associated with resistance to infection by B. bigemina. Discussion: The Crioulo Lageano breed has alleles that may confer resistance against infection by A. marginale and B. bovis.Fil: da Silva Casa, Mariana. Universidade Federal de Santa Catarina; BrasilFil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; ArgentinaFil: Fonteque, Graziela Vieira. Universidade Federal de Santa Catarina; BrasilFil: Miguel, Ellen Lara. Universidade Federal de Santa Catarina; BrasilFil: Vogel, Carla Ivane Ganz. Universidade Federal de Santa Catarina; BrasilFil: Miletti, Luiz Claudio. Universidade Federal de Santa Catarina; BrasilFil: Takeshima, Shin Nosuke. Jumonji University; JapónFil: Fonteque, Joandes Henrique. Universidade Federal de Santa Catarina; BrasilFrontiers Media2023-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/253694da Silva Casa, Mariana; Giovambattista, Guillermo; Fonteque, Graziela Vieira; Miguel, Ellen Lara; Vogel, Carla Ivane Ganz; et al.; Identification of Anaplasma marginale, Babesia bovis and Babesia bigemina resistance alleles in Crioulo Lageano cattle using PCR-SBT and BoLA-DRB3 gene sequencing; Frontiers Media; Frontiers in Veterinary Science; 10; 9-2023; 1-62297-1769CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3389/fvets.2023.1256928info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2023.1256928/fullinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:45:28Zoai:ri.conicet.gov.ar:11336/253694instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:45:28.334CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Identification of Anaplasma marginale, Babesia bovis and Babesia bigemina resistance alleles in Crioulo Lageano cattle using PCR-SBT and BoLA-DRB3 gene sequencing
title Identification of Anaplasma marginale, Babesia bovis and Babesia bigemina resistance alleles in Crioulo Lageano cattle using PCR-SBT and BoLA-DRB3 gene sequencing
spellingShingle Identification of Anaplasma marginale, Babesia bovis and Babesia bigemina resistance alleles in Crioulo Lageano cattle using PCR-SBT and BoLA-DRB3 gene sequencing
da Silva Casa, Mariana
ALLELE
ANAPLASMA
BABESIA
CREOLE BREED
PCR
title_short Identification of Anaplasma marginale, Babesia bovis and Babesia bigemina resistance alleles in Crioulo Lageano cattle using PCR-SBT and BoLA-DRB3 gene sequencing
title_full Identification of Anaplasma marginale, Babesia bovis and Babesia bigemina resistance alleles in Crioulo Lageano cattle using PCR-SBT and BoLA-DRB3 gene sequencing
title_fullStr Identification of Anaplasma marginale, Babesia bovis and Babesia bigemina resistance alleles in Crioulo Lageano cattle using PCR-SBT and BoLA-DRB3 gene sequencing
title_full_unstemmed Identification of Anaplasma marginale, Babesia bovis and Babesia bigemina resistance alleles in Crioulo Lageano cattle using PCR-SBT and BoLA-DRB3 gene sequencing
title_sort Identification of Anaplasma marginale, Babesia bovis and Babesia bigemina resistance alleles in Crioulo Lageano cattle using PCR-SBT and BoLA-DRB3 gene sequencing
dc.creator.none.fl_str_mv da Silva Casa, Mariana
Giovambattista, Guillermo
Fonteque, Graziela Vieira
Miguel, Ellen Lara
Vogel, Carla Ivane Ganz
Miletti, Luiz Claudio
Takeshima, Shin Nosuke
Fonteque, Joandes Henrique
author da Silva Casa, Mariana
author_facet da Silva Casa, Mariana
Giovambattista, Guillermo
Fonteque, Graziela Vieira
Miguel, Ellen Lara
Vogel, Carla Ivane Ganz
Miletti, Luiz Claudio
Takeshima, Shin Nosuke
Fonteque, Joandes Henrique
author_role author
author2 Giovambattista, Guillermo
Fonteque, Graziela Vieira
Miguel, Ellen Lara
Vogel, Carla Ivane Ganz
Miletti, Luiz Claudio
Takeshima, Shin Nosuke
Fonteque, Joandes Henrique
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ALLELE
ANAPLASMA
BABESIA
CREOLE BREED
PCR
topic ALLELE
ANAPLASMA
BABESIA
CREOLE BREED
PCR
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Introduction: The BoLA-DRB3 gene in cattle is associated with tolerance to several infectious diseases, such as neosporosis, dermatophilosis, leukosis, and mastitis. Methods: This study used PCR-SBT and BoLA-DRB3 gene sequencing to determine the association between the presence or absence of Anaplasma marginale, Babesia bovis, and Babesia bigemina infections in 208 Crioulo Lageano cattle and alleles present in the population. The chi-square test and odds ratio analysis were employed to establish the association. Results: Of the BoLA-DRB3 gene alleles present in the population, two alleles were significantly associated with resistance to A. marginale infections: BoLA-DRB3001:01 (p < 0.001; OR = 0.224), which had a frequency of 7.93%, and BoLA-DRB3024:06 (p = 0.007; OR < 0.00001), which had a frequency of 0.72%. Regarding B. bovis infection, the BoLA-DRB3*011:01 allele (p = 0.002; OR = 0.271) had a frequency of 6% in the population and was associated with resistance to the infection. None of the alleles was associated with resistance to infection by B. bigemina. Discussion: The Crioulo Lageano breed has alleles that may confer resistance against infection by A. marginale and B. bovis.
Fil: da Silva Casa, Mariana. Universidade Federal de Santa Catarina; Brasil
Fil: Giovambattista, Guillermo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico CONICET- La Plata. Instituto de Genética Veterinaria "Ing. Fernando Noel Dulout". Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Instituto de Genética Veterinaria; Argentina
Fil: Fonteque, Graziela Vieira. Universidade Federal de Santa Catarina; Brasil
Fil: Miguel, Ellen Lara. Universidade Federal de Santa Catarina; Brasil
Fil: Vogel, Carla Ivane Ganz. Universidade Federal de Santa Catarina; Brasil
Fil: Miletti, Luiz Claudio. Universidade Federal de Santa Catarina; Brasil
Fil: Takeshima, Shin Nosuke. Jumonji University; Japón
Fil: Fonteque, Joandes Henrique. Universidade Federal de Santa Catarina; Brasil
description Introduction: The BoLA-DRB3 gene in cattle is associated with tolerance to several infectious diseases, such as neosporosis, dermatophilosis, leukosis, and mastitis. Methods: This study used PCR-SBT and BoLA-DRB3 gene sequencing to determine the association between the presence or absence of Anaplasma marginale, Babesia bovis, and Babesia bigemina infections in 208 Crioulo Lageano cattle and alleles present in the population. The chi-square test and odds ratio analysis were employed to establish the association. Results: Of the BoLA-DRB3 gene alleles present in the population, two alleles were significantly associated with resistance to A. marginale infections: BoLA-DRB3001:01 (p < 0.001; OR = 0.224), which had a frequency of 7.93%, and BoLA-DRB3024:06 (p = 0.007; OR < 0.00001), which had a frequency of 0.72%. Regarding B. bovis infection, the BoLA-DRB3*011:01 allele (p = 0.002; OR = 0.271) had a frequency of 6% in the population and was associated with resistance to the infection. None of the alleles was associated with resistance to infection by B. bigemina. Discussion: The Crioulo Lageano breed has alleles that may confer resistance against infection by A. marginale and B. bovis.
publishDate 2023
dc.date.none.fl_str_mv 2023-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/253694
da Silva Casa, Mariana; Giovambattista, Guillermo; Fonteque, Graziela Vieira; Miguel, Ellen Lara; Vogel, Carla Ivane Ganz; et al.; Identification of Anaplasma marginale, Babesia bovis and Babesia bigemina resistance alleles in Crioulo Lageano cattle using PCR-SBT and BoLA-DRB3 gene sequencing; Frontiers Media; Frontiers in Veterinary Science; 10; 9-2023; 1-6
2297-1769
CONICET Digital
CONICET
url http://hdl.handle.net/11336/253694
identifier_str_mv da Silva Casa, Mariana; Giovambattista, Guillermo; Fonteque, Graziela Vieira; Miguel, Ellen Lara; Vogel, Carla Ivane Ganz; et al.; Identification of Anaplasma marginale, Babesia bovis and Babesia bigemina resistance alleles in Crioulo Lageano cattle using PCR-SBT and BoLA-DRB3 gene sequencing; Frontiers Media; Frontiers in Veterinary Science; 10; 9-2023; 1-6
2297-1769
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fvets.2023.1256928
info:eu-repo/semantics/altIdentifier/url/https://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2023.1256928/full
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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