Allele-specific expression of a weeping lovegrass gene from the lignin biosynthetic pathway, caffeoyl-coenzyme A 3-O-methyltransferase

Autores
Díaz, Marina Lucía; Garbus, Ingrid; Echenique, Carmen Viviana
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Eragrostis curvula is an important forage grass in marginal regions for its capacity to grow and develop in sandy, not very fertile soils and for its drought tolerance. However, its widespread use for animal production is limited at present since it has low forage quality. In forage species, lignin content has been recognized as one of the main factors that affects this parameter. The O-methylation at the C3 position of the phenolic ring of caffeic acid constitutes a key step in the lignin biosynthetic pathway. The enzyme caffeoyl-CoA 3-O-methyltransferase (CCoAOMT)catalyzes such methylation and is thus considered an interesting target for molecular breeding programs. Here we report the isolation of the full-length CCoAOMT cDNA from the E. curvula inflorescences library. Primers based on this sequence led to the amplification of seven unigenes from genomic DNA from cvs. Tanganyika, Don Pablo and Kromdraai.
Fil: Díaz, Marina Lucía. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Garbus, Ingrid. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Echenique, Carmen Viviana. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Materia
CCoAOMT gene
Lignin
Weeping lovegrass
Allele expression
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/246038
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Allele-specific expression of a weeping lovegrass gene from the lignin biosynthetic pathway, caffeoyl-coenzyme A 3-O-methyltransferaseDíaz, Marina LucíaGarbus, IngridEchenique, Carmen VivianaCCoAOMT geneLigninWeeping lovegrassAllele expressionhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Eragrostis curvula is an important forage grass in marginal regions for its capacity to grow and develop in sandy, not very fertile soils and for its drought tolerance. However, its widespread use for animal production is limited at present since it has low forage quality. In forage species, lignin content has been recognized as one of the main factors that affects this parameter. The O-methylation at the C3 position of the phenolic ring of caffeic acid constitutes a key step in the lignin biosynthetic pathway. The enzyme caffeoyl-CoA 3-O-methyltransferase (CCoAOMT)catalyzes such methylation and is thus considered an interesting target for molecular breeding programs. Here we report the isolation of the full-length CCoAOMT cDNA from the E. curvula inflorescences library. Primers based on this sequence led to the amplification of seven unigenes from genomic DNA from cvs. Tanganyika, Don Pablo and Kromdraai.Fil: Díaz, Marina Lucía. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Garbus, Ingrid. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaFil: Echenique, Carmen Viviana. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; ArgentinaSpringer2010-02-24info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/246038Díaz, Marina Lucía; Garbus, Ingrid; Echenique, Carmen Viviana; Allele-specific expression of a weeping lovegrass gene from the lignin biosynthetic pathway, caffeoyl-coenzyme A 3-O-methyltransferase; Springer; Molecular Breeding; 26; 4; 24-2-2010; 627-6371380-3743CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11032-010-9399-zinfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11032-010-9399-zinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:46:43Zoai:ri.conicet.gov.ar:11336/246038instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:46:43.742CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Allele-specific expression of a weeping lovegrass gene from the lignin biosynthetic pathway, caffeoyl-coenzyme A 3-O-methyltransferase
title Allele-specific expression of a weeping lovegrass gene from the lignin biosynthetic pathway, caffeoyl-coenzyme A 3-O-methyltransferase
spellingShingle Allele-specific expression of a weeping lovegrass gene from the lignin biosynthetic pathway, caffeoyl-coenzyme A 3-O-methyltransferase
Díaz, Marina Lucía
CCoAOMT gene
Lignin
Weeping lovegrass
Allele expression
title_short Allele-specific expression of a weeping lovegrass gene from the lignin biosynthetic pathway, caffeoyl-coenzyme A 3-O-methyltransferase
title_full Allele-specific expression of a weeping lovegrass gene from the lignin biosynthetic pathway, caffeoyl-coenzyme A 3-O-methyltransferase
title_fullStr Allele-specific expression of a weeping lovegrass gene from the lignin biosynthetic pathway, caffeoyl-coenzyme A 3-O-methyltransferase
title_full_unstemmed Allele-specific expression of a weeping lovegrass gene from the lignin biosynthetic pathway, caffeoyl-coenzyme A 3-O-methyltransferase
title_sort Allele-specific expression of a weeping lovegrass gene from the lignin biosynthetic pathway, caffeoyl-coenzyme A 3-O-methyltransferase
dc.creator.none.fl_str_mv Díaz, Marina Lucía
Garbus, Ingrid
Echenique, Carmen Viviana
author Díaz, Marina Lucía
author_facet Díaz, Marina Lucía
Garbus, Ingrid
Echenique, Carmen Viviana
author_role author
author2 Garbus, Ingrid
Echenique, Carmen Viviana
author2_role author
author
dc.subject.none.fl_str_mv CCoAOMT gene
Lignin
Weeping lovegrass
Allele expression
topic CCoAOMT gene
Lignin
Weeping lovegrass
Allele expression
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Eragrostis curvula is an important forage grass in marginal regions for its capacity to grow and develop in sandy, not very fertile soils and for its drought tolerance. However, its widespread use for animal production is limited at present since it has low forage quality. In forage species, lignin content has been recognized as one of the main factors that affects this parameter. The O-methylation at the C3 position of the phenolic ring of caffeic acid constitutes a key step in the lignin biosynthetic pathway. The enzyme caffeoyl-CoA 3-O-methyltransferase (CCoAOMT)catalyzes such methylation and is thus considered an interesting target for molecular breeding programs. Here we report the isolation of the full-length CCoAOMT cDNA from the E. curvula inflorescences library. Primers based on this sequence led to the amplification of seven unigenes from genomic DNA from cvs. Tanganyika, Don Pablo and Kromdraai.
Fil: Díaz, Marina Lucía. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Garbus, Ingrid. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
Fil: Echenique, Carmen Viviana. Universidad Nacional del Sur. Departamento de Agronomía; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Centro de Recursos Naturales Renovables de la Zona Semiárida. Universidad Nacional del Sur. Centro de Recursos Naturales Renovables de la Zona Semiárida; Argentina
description Eragrostis curvula is an important forage grass in marginal regions for its capacity to grow and develop in sandy, not very fertile soils and for its drought tolerance. However, its widespread use for animal production is limited at present since it has low forage quality. In forage species, lignin content has been recognized as one of the main factors that affects this parameter. The O-methylation at the C3 position of the phenolic ring of caffeic acid constitutes a key step in the lignin biosynthetic pathway. The enzyme caffeoyl-CoA 3-O-methyltransferase (CCoAOMT)catalyzes such methylation and is thus considered an interesting target for molecular breeding programs. Here we report the isolation of the full-length CCoAOMT cDNA from the E. curvula inflorescences library. Primers based on this sequence led to the amplification of seven unigenes from genomic DNA from cvs. Tanganyika, Don Pablo and Kromdraai.
publishDate 2010
dc.date.none.fl_str_mv 2010-02-24
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/246038
Díaz, Marina Lucía; Garbus, Ingrid; Echenique, Carmen Viviana; Allele-specific expression of a weeping lovegrass gene from the lignin biosynthetic pathway, caffeoyl-coenzyme A 3-O-methyltransferase; Springer; Molecular Breeding; 26; 4; 24-2-2010; 627-637
1380-3743
CONICET Digital
CONICET
url http://hdl.handle.net/11336/246038
identifier_str_mv Díaz, Marina Lucía; Garbus, Ingrid; Echenique, Carmen Viviana; Allele-specific expression of a weeping lovegrass gene from the lignin biosynthetic pathway, caffeoyl-coenzyme A 3-O-methyltransferase; Springer; Molecular Breeding; 26; 4; 24-2-2010; 627-637
1380-3743
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s11032-010-9399-z
info:eu-repo/semantics/altIdentifier/doi/10.1007/s11032-010-9399-z
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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