Nano-hydroxyapatite for use in bone tissue repair

Autores
D'elía, Noelia Laura; Gravina, Noel; Laiuppa, Juan Andrés; Santillán, Graciela Edith; Messina, Paula Verónica
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Bone can be considered as a biological hybrid material composed of organic and inorganic components: collagen and rod-shaped hydroxyapatite (HAP) of 20–50 nm lengths. The synthetic hydroxyapatite (Ca10(PO4)6(OH)2) has been extensively used as a bone substitute material due to its chemical and structural similarities with natural mineral bone. One way to obtain HAP nanoparticles is by using self-assembled amphiphilic molecules as structure directors. This study involves different hexadecyltrimethylammonium bromide (CTAB) micellar-block copolymer organized networks. Inorganic precursors were added in sequence to the CTAB-polymer solution, followed by a hydrothermal treatment. The final product was separated from the suspension by filtration and then dried. The X-ray diffraction and infrared spectroscopy pattern of the materials synthesized corresponds to the HAP pattern. Transmission and scanning electron microscopy microphotographs show a fiber network composed by 37 nm length HAP nanorods. After treatment with simulated body fluid (SBF) a layer of HAP nanocrystals grew on the material surface; that is related to the bioactivity of the material. To confirm the samples' biocompatibility, calvarial osteoblasts obtained from neonatal rats were exposed to the material and then, viability and cell adhesion were evaluated. A new method of HAP nanocrystals with similar shape, morphology and chemical characteristics of bone were developed. After SBF immersion, material revealed a spherulitic-like HAP layer that implies a positive physiological response and good bond ability to the host tissue. Therefore, nanomaterials obtained by the proposed synthesis could have a wide range of biomedical applications.
Fil: D'elía, Noelia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Gravina, Noel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Laiuppa, Juan Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Santillán, Graciela Edith. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Messina, Paula Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Materia
Hydroxyapatite
Ctab
Bioactivity
Biomedical-Applications
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/40475
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/40475
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Nano-hydroxyapatite for use in bone tissue repairD'elía, Noelia LauraGravina, NoelLaiuppa, Juan AndrésSantillán, Graciela EdithMessina, Paula VerónicaHydroxyapatiteCtabBioactivityBiomedical-Applicationshttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1https://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Bone can be considered as a biological hybrid material composed of organic and inorganic components: collagen and rod-shaped hydroxyapatite (HAP) of 20–50 nm lengths. The synthetic hydroxyapatite (Ca10(PO4)6(OH)2) has been extensively used as a bone substitute material due to its chemical and structural similarities with natural mineral bone. One way to obtain HAP nanoparticles is by using self-assembled amphiphilic molecules as structure directors. This study involves different hexadecyltrimethylammonium bromide (CTAB) micellar-block copolymer organized networks. Inorganic precursors were added in sequence to the CTAB-polymer solution, followed by a hydrothermal treatment. The final product was separated from the suspension by filtration and then dried. The X-ray diffraction and infrared spectroscopy pattern of the materials synthesized corresponds to the HAP pattern. Transmission and scanning electron microscopy microphotographs show a fiber network composed by 37 nm length HAP nanorods. After treatment with simulated body fluid (SBF) a layer of HAP nanocrystals grew on the material surface; that is related to the bioactivity of the material. To confirm the samples' biocompatibility, calvarial osteoblasts obtained from neonatal rats were exposed to the material and then, viability and cell adhesion were evaluated. A new method of HAP nanocrystals with similar shape, morphology and chemical characteristics of bone were developed. After SBF immersion, material revealed a spherulitic-like HAP layer that implies a positive physiological response and good bond ability to the host tissue. Therefore, nanomaterials obtained by the proposed synthesis could have a wide range of biomedical applications.Fil: D'elía, Noelia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Gravina, Noel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaFil: Laiuppa, Juan Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Santillán, Graciela Edith. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; ArgentinaFil: Messina, Paula Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; ArgentinaElsevier Science Inc2015-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/40475D'elía, Noelia Laura; Gravina, Noel; Laiuppa, Juan Andrés; Santillán, Graciela Edith; Messina, Paula Verónica; Nano-hydroxyapatite for use in bone tissue repair; Elsevier Science Inc; Bone; 71; 2-2015; 260-2608756-3282CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.bone.2014.03.026info:eu-repo/semantics/altIdentifier/url/http://www.thebonejournal.com/article/S8756-3282(14)00100-8/fulltextinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:56:32Zoai:ri.conicet.gov.ar:11336/40475instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:56:32.762CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Nano-hydroxyapatite for use in bone tissue repair
title Nano-hydroxyapatite for use in bone tissue repair
spellingShingle Nano-hydroxyapatite for use in bone tissue repair
D'elía, Noelia Laura
Hydroxyapatite
Ctab
Bioactivity
Biomedical-Applications
title_short Nano-hydroxyapatite for use in bone tissue repair
title_full Nano-hydroxyapatite for use in bone tissue repair
title_fullStr Nano-hydroxyapatite for use in bone tissue repair
title_full_unstemmed Nano-hydroxyapatite for use in bone tissue repair
title_sort Nano-hydroxyapatite for use in bone tissue repair
dc.creator.none.fl_str_mv D'elía, Noelia Laura
Gravina, Noel
Laiuppa, Juan Andrés
Santillán, Graciela Edith
Messina, Paula Verónica
author D'elía, Noelia Laura
author_facet D'elía, Noelia Laura
Gravina, Noel
Laiuppa, Juan Andrés
Santillán, Graciela Edith
Messina, Paula Verónica
author_role author
author2 Gravina, Noel
Laiuppa, Juan Andrés
Santillán, Graciela Edith
Messina, Paula Verónica
author2_role author
author
author
author
dc.subject.none.fl_str_mv Hydroxyapatite
Ctab
Bioactivity
Biomedical-Applications
topic Hydroxyapatite
Ctab
Bioactivity
Biomedical-Applications
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Bone can be considered as a biological hybrid material composed of organic and inorganic components: collagen and rod-shaped hydroxyapatite (HAP) of 20–50 nm lengths. The synthetic hydroxyapatite (Ca10(PO4)6(OH)2) has been extensively used as a bone substitute material due to its chemical and structural similarities with natural mineral bone. One way to obtain HAP nanoparticles is by using self-assembled amphiphilic molecules as structure directors. This study involves different hexadecyltrimethylammonium bromide (CTAB) micellar-block copolymer organized networks. Inorganic precursors were added in sequence to the CTAB-polymer solution, followed by a hydrothermal treatment. The final product was separated from the suspension by filtration and then dried. The X-ray diffraction and infrared spectroscopy pattern of the materials synthesized corresponds to the HAP pattern. Transmission and scanning electron microscopy microphotographs show a fiber network composed by 37 nm length HAP nanorods. After treatment with simulated body fluid (SBF) a layer of HAP nanocrystals grew on the material surface; that is related to the bioactivity of the material. To confirm the samples' biocompatibility, calvarial osteoblasts obtained from neonatal rats were exposed to the material and then, viability and cell adhesion were evaluated. A new method of HAP nanocrystals with similar shape, morphology and chemical characteristics of bone were developed. After SBF immersion, material revealed a spherulitic-like HAP layer that implies a positive physiological response and good bond ability to the host tissue. Therefore, nanomaterials obtained by the proposed synthesis could have a wide range of biomedical applications.
Fil: D'elía, Noelia Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Gravina, Noel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
Fil: Laiuppa, Juan Andrés. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Santillán, Graciela Edith. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional del Sur. Departamento de Biología, Bioquímica y Farmacia; Argentina
Fil: Messina, Paula Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Bahía Blanca. Instituto de Química del Sur. Universidad Nacional del Sur. Departamento de Química. Instituto de Química del Sur; Argentina
description Bone can be considered as a biological hybrid material composed of organic and inorganic components: collagen and rod-shaped hydroxyapatite (HAP) of 20–50 nm lengths. The synthetic hydroxyapatite (Ca10(PO4)6(OH)2) has been extensively used as a bone substitute material due to its chemical and structural similarities with natural mineral bone. One way to obtain HAP nanoparticles is by using self-assembled amphiphilic molecules as structure directors. This study involves different hexadecyltrimethylammonium bromide (CTAB) micellar-block copolymer organized networks. Inorganic precursors were added in sequence to the CTAB-polymer solution, followed by a hydrothermal treatment. The final product was separated from the suspension by filtration and then dried. The X-ray diffraction and infrared spectroscopy pattern of the materials synthesized corresponds to the HAP pattern. Transmission and scanning electron microscopy microphotographs show a fiber network composed by 37 nm length HAP nanorods. After treatment with simulated body fluid (SBF) a layer of HAP nanocrystals grew on the material surface; that is related to the bioactivity of the material. To confirm the samples' biocompatibility, calvarial osteoblasts obtained from neonatal rats were exposed to the material and then, viability and cell adhesion were evaluated. A new method of HAP nanocrystals with similar shape, morphology and chemical characteristics of bone were developed. After SBF immersion, material revealed a spherulitic-like HAP layer that implies a positive physiological response and good bond ability to the host tissue. Therefore, nanomaterials obtained by the proposed synthesis could have a wide range of biomedical applications.
publishDate 2015
dc.date.none.fl_str_mv 2015-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/40475
D'elía, Noelia Laura; Gravina, Noel; Laiuppa, Juan Andrés; Santillán, Graciela Edith; Messina, Paula Verónica; Nano-hydroxyapatite for use in bone tissue repair; Elsevier Science Inc; Bone; 71; 2-2015; 260-260
8756-3282
CONICET Digital
CONICET
url http://hdl.handle.net/11336/40475
identifier_str_mv D'elía, Noelia Laura; Gravina, Noel; Laiuppa, Juan Andrés; Santillán, Graciela Edith; Messina, Paula Verónica; Nano-hydroxyapatite for use in bone tissue repair; Elsevier Science Inc; Bone; 71; 2-2015; 260-260
8756-3282
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.bone.2014.03.026
info:eu-repo/semantics/altIdentifier/url/http://www.thebonejournal.com/article/S8756-3282(14)00100-8/fulltext
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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