Titanium-hydroxyapatite composites sintered at low temperature for tissue engineering: In vitro cell support and biocompatibility

Autores
Comin, Romina; Cid, Mariana Paula; Grinschpun, Luciano; Oldani, Carlos Rodolfo; Salvatierra, Nancy Alicia
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In clinical orthopedics, a critical problem is the bone tissue loss produced by a disease or injury. The use of composites from titanium and hydroxyapatite for biomedical applications has increased due to the resulting advantageous combination of hydroxyapatite bioactivity and favorable mechanical properties of titanium. Powder metallurgy is a simple and lower-cost method that uses powder from titanium and hydroxyapatite to obtain composites having hydroxyapatite phases in a metallic matrix. However, this method has certain limitations arising from thermal decomposition of hydroxyapatite in the titanium-hydroxyapatite system above 800°C. We obtained a composite from titanium and bovine hydroxyapatite powders sintered at 800°C and evaluated its bioactivity and cytocompatibility according to the ISO 10993 standard. Methods: Surface analysis and bioactivity of the composite was evaluated by X-ray diffraction and SEM. MTT assay was carried out to assess cytotoxicity on Vero and NIH3T3 cells. Cell morphology and cell adhesion on the composite surface were analyzed using fluorescence and SEM. Results: We obtained a porous composite with hydroxyapatite particles well integrated in titanium matrix which presented excellent bioactivity. Our data did not reveal any toxicity of titanium-hydroxyapatite composite on Vero or NIH3T3 cells. Moreover, extracts from composite did not affect cell morphology or density. Finally, NIH3T3 cells were capable of adhering to and proliferating on the composite surface. Conclusions: The composite obtained displayed promising biomedical applications through the simple method of powder metallurgy. Additionally, these findings provide an in vitro proof for adequate biocompatibility of titanium-hydroxyapatite composite sintered at 800°C.
Fil: Comin, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Cid, Mariana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Grinschpun, Luciano. Universidad Nacional de Córdoba. Facultad de Cs.exactas Físicas y Naturales. Departamento de Materiales y Tecnología; Argentina
Fil: Oldani, Carlos Rodolfo. Universidad Nacional de Córdoba. Facultad de Cs.exactas Físicas y Naturales. Departamento de Materiales y Tecnología; Argentina
Fil: Salvatierra, Nancy Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Materia
BIOCOMPATIBILITY
COMPOSITE
HYDROXYAPATITE
POWDER METALLURGY
TITANIUM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/63872
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/63872
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Titanium-hydroxyapatite composites sintered at low temperature for tissue engineering: In vitro cell support and biocompatibilityComin, RominaCid, Mariana PaulaGrinschpun, LucianoOldani, Carlos RodolfoSalvatierra, Nancy AliciaBIOCOMPATIBILITYCOMPOSITEHYDROXYAPATITEPOWDER METALLURGYTITANIUMIn clinical orthopedics, a critical problem is the bone tissue loss produced by a disease or injury. The use of composites from titanium and hydroxyapatite for biomedical applications has increased due to the resulting advantageous combination of hydroxyapatite bioactivity and favorable mechanical properties of titanium. Powder metallurgy is a simple and lower-cost method that uses powder from titanium and hydroxyapatite to obtain composites having hydroxyapatite phases in a metallic matrix. However, this method has certain limitations arising from thermal decomposition of hydroxyapatite in the titanium-hydroxyapatite system above 800°C. We obtained a composite from titanium and bovine hydroxyapatite powders sintered at 800°C and evaluated its bioactivity and cytocompatibility according to the ISO 10993 standard. Methods: Surface analysis and bioactivity of the composite was evaluated by X-ray diffraction and SEM. MTT assay was carried out to assess cytotoxicity on Vero and NIH3T3 cells. Cell morphology and cell adhesion on the composite surface were analyzed using fluorescence and SEM. Results: We obtained a porous composite with hydroxyapatite particles well integrated in titanium matrix which presented excellent bioactivity. Our data did not reveal any toxicity of titanium-hydroxyapatite composite on Vero or NIH3T3 cells. Moreover, extracts from composite did not affect cell morphology or density. Finally, NIH3T3 cells were capable of adhering to and proliferating on the composite surface. Conclusions: The composite obtained displayed promising biomedical applications through the simple method of powder metallurgy. Additionally, these findings provide an in vitro proof for adequate biocompatibility of titanium-hydroxyapatite composite sintered at 800°C.Fil: Comin, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Cid, Mariana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaFil: Grinschpun, Luciano. Universidad Nacional de Córdoba. Facultad de Cs.exactas Físicas y Naturales. Departamento de Materiales y Tecnología; ArgentinaFil: Oldani, Carlos Rodolfo. Universidad Nacional de Córdoba. Facultad de Cs.exactas Físicas y Naturales. Departamento de Materiales y Tecnología; ArgentinaFil: Salvatierra, Nancy Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaWichtig Publishing Srl2017-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/63872Comin, Romina; Cid, Mariana Paula; Grinschpun, Luciano; Oldani, Carlos Rodolfo; Salvatierra, Nancy Alicia; Titanium-hydroxyapatite composites sintered at low temperature for tissue engineering: In vitro cell support and biocompatibility; Wichtig Publishing Srl; Journal of Applied Biomaterials and Functional Materials; 15; 2; 4-2017; e176-e1832280-8000CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/10.5301/jabfm.5000340info:eu-repo/semantics/altIdentifier/doi/10.5301/jabfm.5000340info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:47:00Zoai:ri.conicet.gov.ar:11336/63872instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:47:00.733CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Titanium-hydroxyapatite composites sintered at low temperature for tissue engineering: In vitro cell support and biocompatibility
title Titanium-hydroxyapatite composites sintered at low temperature for tissue engineering: In vitro cell support and biocompatibility
spellingShingle Titanium-hydroxyapatite composites sintered at low temperature for tissue engineering: In vitro cell support and biocompatibility
Comin, Romina
BIOCOMPATIBILITY
COMPOSITE
HYDROXYAPATITE
POWDER METALLURGY
TITANIUM
title_short Titanium-hydroxyapatite composites sintered at low temperature for tissue engineering: In vitro cell support and biocompatibility
title_full Titanium-hydroxyapatite composites sintered at low temperature for tissue engineering: In vitro cell support and biocompatibility
title_fullStr Titanium-hydroxyapatite composites sintered at low temperature for tissue engineering: In vitro cell support and biocompatibility
title_full_unstemmed Titanium-hydroxyapatite composites sintered at low temperature for tissue engineering: In vitro cell support and biocompatibility
title_sort Titanium-hydroxyapatite composites sintered at low temperature for tissue engineering: In vitro cell support and biocompatibility
dc.creator.none.fl_str_mv Comin, Romina
Cid, Mariana Paula
Grinschpun, Luciano
Oldani, Carlos Rodolfo
Salvatierra, Nancy Alicia
author Comin, Romina
author_facet Comin, Romina
Cid, Mariana Paula
Grinschpun, Luciano
Oldani, Carlos Rodolfo
Salvatierra, Nancy Alicia
author_role author
author2 Cid, Mariana Paula
Grinschpun, Luciano
Oldani, Carlos Rodolfo
Salvatierra, Nancy Alicia
author2_role author
author
author
author
dc.subject.none.fl_str_mv BIOCOMPATIBILITY
COMPOSITE
HYDROXYAPATITE
POWDER METALLURGY
TITANIUM
topic BIOCOMPATIBILITY
COMPOSITE
HYDROXYAPATITE
POWDER METALLURGY
TITANIUM
dc.description.none.fl_txt_mv In clinical orthopedics, a critical problem is the bone tissue loss produced by a disease or injury. The use of composites from titanium and hydroxyapatite for biomedical applications has increased due to the resulting advantageous combination of hydroxyapatite bioactivity and favorable mechanical properties of titanium. Powder metallurgy is a simple and lower-cost method that uses powder from titanium and hydroxyapatite to obtain composites having hydroxyapatite phases in a metallic matrix. However, this method has certain limitations arising from thermal decomposition of hydroxyapatite in the titanium-hydroxyapatite system above 800°C. We obtained a composite from titanium and bovine hydroxyapatite powders sintered at 800°C and evaluated its bioactivity and cytocompatibility according to the ISO 10993 standard. Methods: Surface analysis and bioactivity of the composite was evaluated by X-ray diffraction and SEM. MTT assay was carried out to assess cytotoxicity on Vero and NIH3T3 cells. Cell morphology and cell adhesion on the composite surface were analyzed using fluorescence and SEM. Results: We obtained a porous composite with hydroxyapatite particles well integrated in titanium matrix which presented excellent bioactivity. Our data did not reveal any toxicity of titanium-hydroxyapatite composite on Vero or NIH3T3 cells. Moreover, extracts from composite did not affect cell morphology or density. Finally, NIH3T3 cells were capable of adhering to and proliferating on the composite surface. Conclusions: The composite obtained displayed promising biomedical applications through the simple method of powder metallurgy. Additionally, these findings provide an in vitro proof for adequate biocompatibility of titanium-hydroxyapatite composite sintered at 800°C.
Fil: Comin, Romina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Cid, Mariana Paula. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fil: Grinschpun, Luciano. Universidad Nacional de Córdoba. Facultad de Cs.exactas Físicas y Naturales. Departamento de Materiales y Tecnología; Argentina
Fil: Oldani, Carlos Rodolfo. Universidad Nacional de Córdoba. Facultad de Cs.exactas Físicas y Naturales. Departamento de Materiales y Tecnología; Argentina
Fil: Salvatierra, Nancy Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
description In clinical orthopedics, a critical problem is the bone tissue loss produced by a disease or injury. The use of composites from titanium and hydroxyapatite for biomedical applications has increased due to the resulting advantageous combination of hydroxyapatite bioactivity and favorable mechanical properties of titanium. Powder metallurgy is a simple and lower-cost method that uses powder from titanium and hydroxyapatite to obtain composites having hydroxyapatite phases in a metallic matrix. However, this method has certain limitations arising from thermal decomposition of hydroxyapatite in the titanium-hydroxyapatite system above 800°C. We obtained a composite from titanium and bovine hydroxyapatite powders sintered at 800°C and evaluated its bioactivity and cytocompatibility according to the ISO 10993 standard. Methods: Surface analysis and bioactivity of the composite was evaluated by X-ray diffraction and SEM. MTT assay was carried out to assess cytotoxicity on Vero and NIH3T3 cells. Cell morphology and cell adhesion on the composite surface were analyzed using fluorescence and SEM. Results: We obtained a porous composite with hydroxyapatite particles well integrated in titanium matrix which presented excellent bioactivity. Our data did not reveal any toxicity of titanium-hydroxyapatite composite on Vero or NIH3T3 cells. Moreover, extracts from composite did not affect cell morphology or density. Finally, NIH3T3 cells were capable of adhering to and proliferating on the composite surface. Conclusions: The composite obtained displayed promising biomedical applications through the simple method of powder metallurgy. Additionally, these findings provide an in vitro proof for adequate biocompatibility of titanium-hydroxyapatite composite sintered at 800°C.
publishDate 2017
dc.date.none.fl_str_mv 2017-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/63872
Comin, Romina; Cid, Mariana Paula; Grinschpun, Luciano; Oldani, Carlos Rodolfo; Salvatierra, Nancy Alicia; Titanium-hydroxyapatite composites sintered at low temperature for tissue engineering: In vitro cell support and biocompatibility; Wichtig Publishing Srl; Journal of Applied Biomaterials and Functional Materials; 15; 2; 4-2017; e176-e183
2280-8000
CONICET Digital
CONICET
url http://hdl.handle.net/11336/63872
identifier_str_mv Comin, Romina; Cid, Mariana Paula; Grinschpun, Luciano; Oldani, Carlos Rodolfo; Salvatierra, Nancy Alicia; Titanium-hydroxyapatite composites sintered at low temperature for tissue engineering: In vitro cell support and biocompatibility; Wichtig Publishing Srl; Journal of Applied Biomaterials and Functional Materials; 15; 2; 4-2017; e176-e183
2280-8000
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://journals.sagepub.com/doi/10.5301/jabfm.5000340
info:eu-repo/semantics/altIdentifier/doi/10.5301/jabfm.5000340
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wichtig Publishing Srl
publisher.none.fl_str_mv Wichtig Publishing Srl
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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