Novel spectrofluorimetric method for boldine alkaloid determination in herbal drugs and phytopharmaceuticals

Autores
Peralta, Cecilia Mariana; Henestrosa, Cecilia; Gil, Raul Andres; Fernandez, Liliana Patricia; Acosta, Maria Gimena
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A new green on-line method for Boldine determination (BOL) in herbal drugs andphytopharmaceuticals, using its native fluorescence in acid media (λex = 282 nm; λem = 373 nm) has been developed. The presented methodology involves for the first time, a flow injection (FI) strategy using a mini-column of multiwalled carbon nanotubes as retention agent coupled with molecular fluorescence. Different parameters influence as sample pH and flow rate, eluent flow rate and composition; on BOL sensitivity and elution time was investigated by multifactorial techniques.Adequate dynamic calibration range (r2 = 0.9993) was obtained over a concentrationinterval of 0.029 ? 27.0 µg mL−1 BOL. The limits of detection (LOD) and quantification (LOQ) were 0.008 and 0.029 µg mL−1 , respectively. The average recoveries in explored samples ranged from 95 % to 103 %. Under optimized conditions, the throughput sample as high as 30 h−1 was achieved with high repeatability performance (99 %). The proposed development represents a useful and valuable tool emulating the analytical efficiency of the official methodologies for quality control of herbal and phytopharmaceutical drugs containing BOL. Moreover, this approach shows advantages respect to low cost, simplicity and environmental and analyst friendly.
Fil: Peralta, Cecilia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Henestrosa, Cecilia. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia; Argentina
Fil: Gil, Raul Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Acosta, Maria Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Materia
Boldine
Flow Injection Analysis-Spectrofluorimetry
Multivariate Optimization
Phytopharmaceuticals Quality Control
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/64762
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/64762
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Novel spectrofluorimetric method for boldine alkaloid determination in herbal drugs and phytopharmaceuticalsPeralta, Cecilia MarianaHenestrosa, CeciliaGil, Raul AndresFernandez, Liliana PatriciaAcosta, Maria GimenaBoldineFlow Injection Analysis-SpectrofluorimetryMultivariate OptimizationPhytopharmaceuticals Quality Controlhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A new green on-line method for Boldine determination (BOL) in herbal drugs andphytopharmaceuticals, using its native fluorescence in acid media (λex = 282 nm; λem = 373 nm) has been developed. The presented methodology involves for the first time, a flow injection (FI) strategy using a mini-column of multiwalled carbon nanotubes as retention agent coupled with molecular fluorescence. Different parameters influence as sample pH and flow rate, eluent flow rate and composition; on BOL sensitivity and elution time was investigated by multifactorial techniques.Adequate dynamic calibration range (r2 = 0.9993) was obtained over a concentrationinterval of 0.029 ? 27.0 µg mL−1 BOL. The limits of detection (LOD) and quantification (LOQ) were 0.008 and 0.029 µg mL−1 , respectively. The average recoveries in explored samples ranged from 95 % to 103 %. Under optimized conditions, the throughput sample as high as 30 h−1 was achieved with high repeatability performance (99 %). The proposed development represents a useful and valuable tool emulating the analytical efficiency of the official methodologies for quality control of herbal and phytopharmaceutical drugs containing BOL. Moreover, this approach shows advantages respect to low cost, simplicity and environmental and analyst friendly.Fil: Peralta, Cecilia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Henestrosa, Cecilia. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia; ArgentinaFil: Gil, Raul Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Acosta, Maria Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaPergamon-Elsevier Science Ltd2017-04-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/64762Peralta, Cecilia Mariana; Henestrosa, Cecilia; Gil, Raul Andres; Fernandez, Liliana Patricia; Acosta, Maria Gimena; Novel spectrofluorimetric method for boldine alkaloid determination in herbal drugs and phytopharmaceuticals; Pergamon-Elsevier Science Ltd; Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy; 184; 21-4-2017; 101-1081386-1425CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S1386142517303086info:eu-repo/semantics/altIdentifier/doi/10.1016/j.saa.2017.04.039info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:31Zoai:ri.conicet.gov.ar:11336/64762instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:31.888CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Novel spectrofluorimetric method for boldine alkaloid determination in herbal drugs and phytopharmaceuticals
title Novel spectrofluorimetric method for boldine alkaloid determination in herbal drugs and phytopharmaceuticals
spellingShingle Novel spectrofluorimetric method for boldine alkaloid determination in herbal drugs and phytopharmaceuticals
Peralta, Cecilia Mariana
Boldine
Flow Injection Analysis-Spectrofluorimetry
Multivariate Optimization
Phytopharmaceuticals Quality Control
title_short Novel spectrofluorimetric method for boldine alkaloid determination in herbal drugs and phytopharmaceuticals
title_full Novel spectrofluorimetric method for boldine alkaloid determination in herbal drugs and phytopharmaceuticals
title_fullStr Novel spectrofluorimetric method for boldine alkaloid determination in herbal drugs and phytopharmaceuticals
title_full_unstemmed Novel spectrofluorimetric method for boldine alkaloid determination in herbal drugs and phytopharmaceuticals
title_sort Novel spectrofluorimetric method for boldine alkaloid determination in herbal drugs and phytopharmaceuticals
dc.creator.none.fl_str_mv Peralta, Cecilia Mariana
Henestrosa, Cecilia
Gil, Raul Andres
Fernandez, Liliana Patricia
Acosta, Maria Gimena
author Peralta, Cecilia Mariana
author_facet Peralta, Cecilia Mariana
Henestrosa, Cecilia
Gil, Raul Andres
Fernandez, Liliana Patricia
Acosta, Maria Gimena
author_role author
author2 Henestrosa, Cecilia
Gil, Raul Andres
Fernandez, Liliana Patricia
Acosta, Maria Gimena
author2_role author
author
author
author
dc.subject.none.fl_str_mv Boldine
Flow Injection Analysis-Spectrofluorimetry
Multivariate Optimization
Phytopharmaceuticals Quality Control
topic Boldine
Flow Injection Analysis-Spectrofluorimetry
Multivariate Optimization
Phytopharmaceuticals Quality Control
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A new green on-line method for Boldine determination (BOL) in herbal drugs andphytopharmaceuticals, using its native fluorescence in acid media (λex = 282 nm; λem = 373 nm) has been developed. The presented methodology involves for the first time, a flow injection (FI) strategy using a mini-column of multiwalled carbon nanotubes as retention agent coupled with molecular fluorescence. Different parameters influence as sample pH and flow rate, eluent flow rate and composition; on BOL sensitivity and elution time was investigated by multifactorial techniques.Adequate dynamic calibration range (r2 = 0.9993) was obtained over a concentrationinterval of 0.029 ? 27.0 µg mL−1 BOL. The limits of detection (LOD) and quantification (LOQ) were 0.008 and 0.029 µg mL−1 , respectively. The average recoveries in explored samples ranged from 95 % to 103 %. Under optimized conditions, the throughput sample as high as 30 h−1 was achieved with high repeatability performance (99 %). The proposed development represents a useful and valuable tool emulating the analytical efficiency of the official methodologies for quality control of herbal and phytopharmaceutical drugs containing BOL. Moreover, this approach shows advantages respect to low cost, simplicity and environmental and analyst friendly.
Fil: Peralta, Cecilia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Henestrosa, Cecilia. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Departamento de Farmacia; Argentina
Fil: Gil, Raul Andres. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Acosta, Maria Gimena. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
description A new green on-line method for Boldine determination (BOL) in herbal drugs andphytopharmaceuticals, using its native fluorescence in acid media (λex = 282 nm; λem = 373 nm) has been developed. The presented methodology involves for the first time, a flow injection (FI) strategy using a mini-column of multiwalled carbon nanotubes as retention agent coupled with molecular fluorescence. Different parameters influence as sample pH and flow rate, eluent flow rate and composition; on BOL sensitivity and elution time was investigated by multifactorial techniques.Adequate dynamic calibration range (r2 = 0.9993) was obtained over a concentrationinterval of 0.029 ? 27.0 µg mL−1 BOL. The limits of detection (LOD) and quantification (LOQ) were 0.008 and 0.029 µg mL−1 , respectively. The average recoveries in explored samples ranged from 95 % to 103 %. Under optimized conditions, the throughput sample as high as 30 h−1 was achieved with high repeatability performance (99 %). The proposed development represents a useful and valuable tool emulating the analytical efficiency of the official methodologies for quality control of herbal and phytopharmaceutical drugs containing BOL. Moreover, this approach shows advantages respect to low cost, simplicity and environmental and analyst friendly.
publishDate 2017
dc.date.none.fl_str_mv 2017-04-21
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/64762
Peralta, Cecilia Mariana; Henestrosa, Cecilia; Gil, Raul Andres; Fernandez, Liliana Patricia; Acosta, Maria Gimena; Novel spectrofluorimetric method for boldine alkaloid determination in herbal drugs and phytopharmaceuticals; Pergamon-Elsevier Science Ltd; Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy; 184; 21-4-2017; 101-108
1386-1425
CONICET Digital
CONICET
url http://hdl.handle.net/11336/64762
identifier_str_mv Peralta, Cecilia Mariana; Henestrosa, Cecilia; Gil, Raul Andres; Fernandez, Liliana Patricia; Acosta, Maria Gimena; Novel spectrofluorimetric method for boldine alkaloid determination in herbal drugs and phytopharmaceuticals; Pergamon-Elsevier Science Ltd; Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy; 184; 21-4-2017; 101-108
1386-1425
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://linkinghub.elsevier.com/retrieve/pii/S1386142517303086
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.saa.2017.04.039
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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