O-(β-hydroxyethyl)rutosides determination by micellar flow injection (FI) spectrofluorimetry

Autores
Fernandez, Liliana Patricia; Peralta, Cecilia Mariana
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A simple, eco friendly, sensitive and economic flow injection spectrofluorimetric method was developed for the determination of O-(β-hydroxyethyl)rutosides. The procedure was based on the use of an anionic surfactant such as sodium dodecyl sulfate to provide an appreciable O-(β- hydroxyethyl)rutosides fluorescence enhancement, increasing considerably the sensitivity of detection. All the variables affecting the fluorescence intensity were studied and optimized. The flow rate was 5 mL/min with detection at 450 nm (after excitation at 346 nm). A linear correlation between drug amount and peak area was established for O-(β-hydroxyethyl)rutosides in the range of 0.01 - 200 μg/mL with a detection limit of 0.001 μg/mL (s/n = 3). Validation processes were performed by recovering studies with satisfactory results. The new methodology can be employed for the routine analysis of O-(β-hydroxyethyl)rutosides in bulks as well as in commercial formulations.
Fil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Fisica; Argentina
Fil: Peralta, Cecilia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; Argentina
Materia
O-(β-HYDROXYETHYL)RUTOSIDES
MICELLAR ENHANCEMENT
FLOW INJECTION
SPECTROFLUORIMETRY
PHARMACEUTICALS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/5766
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling O-(β-hydroxyethyl)rutosides determination by micellar flow injection (FI) spectrofluorimetryFernandez, Liliana PatriciaPeralta, Cecilia MarianaO-(β-HYDROXYETHYL)RUTOSIDESMICELLAR ENHANCEMENTFLOW INJECTIONSPECTROFLUORIMETRYPHARMACEUTICALShttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1A simple, eco friendly, sensitive and economic flow injection spectrofluorimetric method was developed for the determination of O-(β-hydroxyethyl)rutosides. The procedure was based on the use of an anionic surfactant such as sodium dodecyl sulfate to provide an appreciable O-(β- hydroxyethyl)rutosides fluorescence enhancement, increasing considerably the sensitivity of detection. All the variables affecting the fluorescence intensity were studied and optimized. The flow rate was 5 mL/min with detection at 450 nm (after excitation at 346 nm). A linear correlation between drug amount and peak area was established for O-(β-hydroxyethyl)rutosides in the range of 0.01 - 200 μg/mL with a detection limit of 0.001 μg/mL (s/n = 3). Validation processes were performed by recovering studies with satisfactory results. The new methodology can be employed for the routine analysis of O-(β-hydroxyethyl)rutosides in bulks as well as in commercial formulations.Fil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Fisica; ArgentinaFil: Peralta, Cecilia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; ArgentinaElsevier2014-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/5766Fernandez, Liliana Patricia; Peralta, Cecilia Mariana; O-(β-hydroxyethyl)rutosides determination by micellar flow injection (FI) spectrofluorimetry; Elsevier; Journal of Pharmaceutical Analysis; 4; 6; 7-2014; 368-3732095-1779enginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S2095177914000628info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jpha.2014.07.002info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:06Zoai:ri.conicet.gov.ar:11336/5766instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:06.51CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv O-(β-hydroxyethyl)rutosides determination by micellar flow injection (FI) spectrofluorimetry
title O-(β-hydroxyethyl)rutosides determination by micellar flow injection (FI) spectrofluorimetry
spellingShingle O-(β-hydroxyethyl)rutosides determination by micellar flow injection (FI) spectrofluorimetry
Fernandez, Liliana Patricia
O-(β-HYDROXYETHYL)RUTOSIDES
MICELLAR ENHANCEMENT
FLOW INJECTION
SPECTROFLUORIMETRY
PHARMACEUTICALS
title_short O-(β-hydroxyethyl)rutosides determination by micellar flow injection (FI) spectrofluorimetry
title_full O-(β-hydroxyethyl)rutosides determination by micellar flow injection (FI) spectrofluorimetry
title_fullStr O-(β-hydroxyethyl)rutosides determination by micellar flow injection (FI) spectrofluorimetry
title_full_unstemmed O-(β-hydroxyethyl)rutosides determination by micellar flow injection (FI) spectrofluorimetry
title_sort O-(β-hydroxyethyl)rutosides determination by micellar flow injection (FI) spectrofluorimetry
dc.creator.none.fl_str_mv Fernandez, Liliana Patricia
Peralta, Cecilia Mariana
author Fernandez, Liliana Patricia
author_facet Fernandez, Liliana Patricia
Peralta, Cecilia Mariana
author_role author
author2 Peralta, Cecilia Mariana
author2_role author
dc.subject.none.fl_str_mv O-(β-HYDROXYETHYL)RUTOSIDES
MICELLAR ENHANCEMENT
FLOW INJECTION
SPECTROFLUORIMETRY
PHARMACEUTICALS
topic O-(β-HYDROXYETHYL)RUTOSIDES
MICELLAR ENHANCEMENT
FLOW INJECTION
SPECTROFLUORIMETRY
PHARMACEUTICALS
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv A simple, eco friendly, sensitive and economic flow injection spectrofluorimetric method was developed for the determination of O-(β-hydroxyethyl)rutosides. The procedure was based on the use of an anionic surfactant such as sodium dodecyl sulfate to provide an appreciable O-(β- hydroxyethyl)rutosides fluorescence enhancement, increasing considerably the sensitivity of detection. All the variables affecting the fluorescence intensity were studied and optimized. The flow rate was 5 mL/min with detection at 450 nm (after excitation at 346 nm). A linear correlation between drug amount and peak area was established for O-(β-hydroxyethyl)rutosides in the range of 0.01 - 200 μg/mL with a detection limit of 0.001 μg/mL (s/n = 3). Validation processes were performed by recovering studies with satisfactory results. The new methodology can be employed for the routine analysis of O-(β-hydroxyethyl)rutosides in bulks as well as in commercial formulations.
Fil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Fisica; Argentina
Fil: Peralta, Cecilia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico San Luis. Instituto de Química de San Luis; Argentina. Universidad Nacional de San Luis. Facultad de Quimica, Bioquimica y Farmacia. Departamento de Quimica. Area de Quimica Analitica; Argentina
description A simple, eco friendly, sensitive and economic flow injection spectrofluorimetric method was developed for the determination of O-(β-hydroxyethyl)rutosides. The procedure was based on the use of an anionic surfactant such as sodium dodecyl sulfate to provide an appreciable O-(β- hydroxyethyl)rutosides fluorescence enhancement, increasing considerably the sensitivity of detection. All the variables affecting the fluorescence intensity were studied and optimized. The flow rate was 5 mL/min with detection at 450 nm (after excitation at 346 nm). A linear correlation between drug amount and peak area was established for O-(β-hydroxyethyl)rutosides in the range of 0.01 - 200 μg/mL with a detection limit of 0.001 μg/mL (s/n = 3). Validation processes were performed by recovering studies with satisfactory results. The new methodology can be employed for the routine analysis of O-(β-hydroxyethyl)rutosides in bulks as well as in commercial formulations.
publishDate 2014
dc.date.none.fl_str_mv 2014-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/5766
Fernandez, Liliana Patricia; Peralta, Cecilia Mariana; O-(β-hydroxyethyl)rutosides determination by micellar flow injection (FI) spectrofluorimetry; Elsevier; Journal of Pharmaceutical Analysis; 4; 6; 7-2014; 368-373
2095-1779
url http://hdl.handle.net/11336/5766
identifier_str_mv Fernandez, Liliana Patricia; Peralta, Cecilia Mariana; O-(β-hydroxyethyl)rutosides determination by micellar flow injection (FI) spectrofluorimetry; Elsevier; Journal of Pharmaceutical Analysis; 4; 6; 7-2014; 368-373
2095-1779
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S2095177914000628
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jpha.2014.07.002
info:eu-repo/semantics/altIdentifier/doi/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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