Online naphazoline quality control by micellar-enhanced spectrofluorimetry

Autores
Peralta, Cecilia Mariana; Silva, Raúl Alejandro; Fernandez, Liliana Patricia; Masi, Adriana Noemi
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to develop a method for online spectrofluorimetric quality control of naphazoline (NPZ) in pharmaceuticals and raw drugs. A combination of a flow-injection analysis (FIA) system with micellar-enhanced fluorescence detection is presented as a powerful alternative for the rapid and sensitive analysis of naphazoline. Since NPZ shows low native fluorescence, the use of an anionic surfactant, such as sodium dodecyl sulphate (SDS), provides a considerable enhancement of fluorescence intensity and the nature of the technique allows a possible and easy adaptation to a FIA system. Using λ exc = 280 nm and λ em = 326 nm, a good linear relationship (LOL) was obtained in the range 0.003-10 μg mL -1 with a detection limit (LOD) of 3 × 10 -4 μg mL -1 (s/n = 3). Parameters related to the nature of the analytical signal and to the FIA manifold were optimized. Satisfactory recoveries were obtained in the analysis of commercial pharmaceutical formulations. The proposed method is simple, accurate and allows for high-speed sampling and considerably shorter analysis times. In addition, it requires inexpensive equipment and reagents and has easy operational conditions and no side effects, thus avoiding environmental pollution through toxic waste.
Fil: Peralta, Cecilia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Silva, Raúl Alejandro. Universidad Nacional de San Luis; Argentina
Fil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Masi, Adriana Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Materia
FLOW INJECTION
MICELLAR ENHANCEMENT
NAPHAZOLINE
PHARMACEUTICALS
SPECTROFLUORIMETRY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/84751
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/84751
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Online naphazoline quality control by micellar-enhanced spectrofluorimetryPeralta, Cecilia MarianaSilva, Raúl AlejandroFernandez, Liliana PatriciaMasi, Adriana NoemiFLOW INJECTIONMICELLAR ENHANCEMENTNAPHAZOLINEPHARMACEUTICALSSPECTROFLUORIMETRYhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The aim of this study was to develop a method for online spectrofluorimetric quality control of naphazoline (NPZ) in pharmaceuticals and raw drugs. A combination of a flow-injection analysis (FIA) system with micellar-enhanced fluorescence detection is presented as a powerful alternative for the rapid and sensitive analysis of naphazoline. Since NPZ shows low native fluorescence, the use of an anionic surfactant, such as sodium dodecyl sulphate (SDS), provides a considerable enhancement of fluorescence intensity and the nature of the technique allows a possible and easy adaptation to a FIA system. Using λ exc = 280 nm and λ em = 326 nm, a good linear relationship (LOL) was obtained in the range 0.003-10 μg mL -1 with a detection limit (LOD) of 3 × 10 -4 μg mL -1 (s/n = 3). Parameters related to the nature of the analytical signal and to the FIA manifold were optimized. Satisfactory recoveries were obtained in the analysis of commercial pharmaceutical formulations. The proposed method is simple, accurate and allows for high-speed sampling and considerably shorter analysis times. In addition, it requires inexpensive equipment and reagents and has easy operational conditions and no side effects, thus avoiding environmental pollution through toxic waste.Fil: Peralta, Cecilia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Silva, Raúl Alejandro. Universidad Nacional de San Luis; ArgentinaFil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaFil: Masi, Adriana Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; ArgentinaJohn Wiley & Sons Ltd2011-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/84751Peralta, Cecilia Mariana; Silva, Raúl Alejandro; Fernandez, Liliana Patricia; Masi, Adriana Noemi; Online naphazoline quality control by micellar-enhanced spectrofluorimetry; John Wiley & Sons Ltd; Luminescence : The Journal Of Biological And Chemical Luminescence; 26; 6; 11-2011; 689-6951522-7235CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/bio.1297info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/bio.1297info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:11:27Zoai:ri.conicet.gov.ar:11336/84751instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:11:27.762CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Online naphazoline quality control by micellar-enhanced spectrofluorimetry
title Online naphazoline quality control by micellar-enhanced spectrofluorimetry
spellingShingle Online naphazoline quality control by micellar-enhanced spectrofluorimetry
Peralta, Cecilia Mariana
FLOW INJECTION
MICELLAR ENHANCEMENT
NAPHAZOLINE
PHARMACEUTICALS
SPECTROFLUORIMETRY
title_short Online naphazoline quality control by micellar-enhanced spectrofluorimetry
title_full Online naphazoline quality control by micellar-enhanced spectrofluorimetry
title_fullStr Online naphazoline quality control by micellar-enhanced spectrofluorimetry
title_full_unstemmed Online naphazoline quality control by micellar-enhanced spectrofluorimetry
title_sort Online naphazoline quality control by micellar-enhanced spectrofluorimetry
dc.creator.none.fl_str_mv Peralta, Cecilia Mariana
Silva, Raúl Alejandro
Fernandez, Liliana Patricia
Masi, Adriana Noemi
author Peralta, Cecilia Mariana
author_facet Peralta, Cecilia Mariana
Silva, Raúl Alejandro
Fernandez, Liliana Patricia
Masi, Adriana Noemi
author_role author
author2 Silva, Raúl Alejandro
Fernandez, Liliana Patricia
Masi, Adriana Noemi
author2_role author
author
author
dc.subject.none.fl_str_mv FLOW INJECTION
MICELLAR ENHANCEMENT
NAPHAZOLINE
PHARMACEUTICALS
SPECTROFLUORIMETRY
topic FLOW INJECTION
MICELLAR ENHANCEMENT
NAPHAZOLINE
PHARMACEUTICALS
SPECTROFLUORIMETRY
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The aim of this study was to develop a method for online spectrofluorimetric quality control of naphazoline (NPZ) in pharmaceuticals and raw drugs. A combination of a flow-injection analysis (FIA) system with micellar-enhanced fluorescence detection is presented as a powerful alternative for the rapid and sensitive analysis of naphazoline. Since NPZ shows low native fluorescence, the use of an anionic surfactant, such as sodium dodecyl sulphate (SDS), provides a considerable enhancement of fluorescence intensity and the nature of the technique allows a possible and easy adaptation to a FIA system. Using λ exc = 280 nm and λ em = 326 nm, a good linear relationship (LOL) was obtained in the range 0.003-10 μg mL -1 with a detection limit (LOD) of 3 × 10 -4 μg mL -1 (s/n = 3). Parameters related to the nature of the analytical signal and to the FIA manifold were optimized. Satisfactory recoveries were obtained in the analysis of commercial pharmaceutical formulations. The proposed method is simple, accurate and allows for high-speed sampling and considerably shorter analysis times. In addition, it requires inexpensive equipment and reagents and has easy operational conditions and no side effects, thus avoiding environmental pollution through toxic waste.
Fil: Peralta, Cecilia Mariana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Silva, Raúl Alejandro. Universidad Nacional de San Luis; Argentina
Fil: Fernandez, Liliana Patricia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
Fil: Masi, Adriana Noemi. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - San Luis. Instituto de Química de San Luis. Universidad Nacional de San Luis. Facultad de Química, Bioquímica y Farmacia. Instituto de Química de San Luis; Argentina
description The aim of this study was to develop a method for online spectrofluorimetric quality control of naphazoline (NPZ) in pharmaceuticals and raw drugs. A combination of a flow-injection analysis (FIA) system with micellar-enhanced fluorescence detection is presented as a powerful alternative for the rapid and sensitive analysis of naphazoline. Since NPZ shows low native fluorescence, the use of an anionic surfactant, such as sodium dodecyl sulphate (SDS), provides a considerable enhancement of fluorescence intensity and the nature of the technique allows a possible and easy adaptation to a FIA system. Using λ exc = 280 nm and λ em = 326 nm, a good linear relationship (LOL) was obtained in the range 0.003-10 μg mL -1 with a detection limit (LOD) of 3 × 10 -4 μg mL -1 (s/n = 3). Parameters related to the nature of the analytical signal and to the FIA manifold were optimized. Satisfactory recoveries were obtained in the analysis of commercial pharmaceutical formulations. The proposed method is simple, accurate and allows for high-speed sampling and considerably shorter analysis times. In addition, it requires inexpensive equipment and reagents and has easy operational conditions and no side effects, thus avoiding environmental pollution through toxic waste.
publishDate 2011
dc.date.none.fl_str_mv 2011-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/84751
Peralta, Cecilia Mariana; Silva, Raúl Alejandro; Fernandez, Liliana Patricia; Masi, Adriana Noemi; Online naphazoline quality control by micellar-enhanced spectrofluorimetry; John Wiley & Sons Ltd; Luminescence : The Journal Of Biological And Chemical Luminescence; 26; 6; 11-2011; 689-695
1522-7235
CONICET Digital
CONICET
url http://hdl.handle.net/11336/84751
identifier_str_mv Peralta, Cecilia Mariana; Silva, Raúl Alejandro; Fernandez, Liliana Patricia; Masi, Adriana Noemi; Online naphazoline quality control by micellar-enhanced spectrofluorimetry; John Wiley & Sons Ltd; Luminescence : The Journal Of Biological And Chemical Luminescence; 26; 6; 11-2011; 689-695
1522-7235
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/bio.1297
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/bio.1297
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons Ltd
publisher.none.fl_str_mv John Wiley & Sons Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842270158961246208
score 13.13397

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