Amino acid content and acetylcholinesterase inhibition of Huperzia saururus infusion and decoction

Autores
Vallejo, Mariana Guadalupe; Dimmer, Jesica Ayelen; Ortega, María Gabriela; Cabrera, Jose Luis; Agnese, Alicia Mariel
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Huperzia saururus (Lam.) Trevis. (Lycopodiaceae), an autochthonous plant species in Argentina, is used as a memory improver in traditional medicine. It was studied for this reason and the purified alkaloid extract did show significant activity on learning and memory. The species is mostly consumed in the form of infusions and decoctions. Objectives: To evaluate the activity of the H. saururus infusion and decoction as inhibitors of acetylcholinesterase (AChE) and to determine the amino acid content in both extracts. Material and methods: Infusion and decoction were purified by ionic exchange chromatography and analyzed by high-performance liquid chromatography HPLC-UV, and the AChE inhibition of these extracts was evaluated by using the Ellman method. Results: Both infusion and decoction exerted strong AChE inhibitory activities (IC50 ¼ 7.2 0.4 and 22.7 5.6 mg/mL, respectively). Among nine amino acids, arginine (Arg) was identified in a concentration greater than 9 mg/100 g of dried aerial parts in both extracts. Discussion and conclusion: This high content of Arg could be considered a contributing factor to the activity on memory previously demonstrated for the H. saururus alkaloid extract, since Arg is implicated indirectly in mnemonic processes as a precursor in nitric oxide synthesis. Thus, the central effect of H. saururus could involve two different mechanisms, the cholinergic mechanism and the nitric oxide pathway.
Fil: Vallejo, Mariana Guadalupe. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina
Fil: Dimmer, Jesica Ayelen. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina
Fil: Ortega, María Gabriela. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina
Fil: Cabrera, Jose Luis. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina
Fil: Agnese, Alicia Mariel. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina
Materia
Anti-Cholinesterase Activity
Arginine
Hplc
Plant Extracts
Quantitation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/9317
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Amino acid content and acetylcholinesterase inhibition of Huperzia saururus infusion and decoctionVallejo, Mariana GuadalupeDimmer, Jesica AyelenOrtega, María GabrielaCabrera, Jose LuisAgnese, Alicia MarielAnti-Cholinesterase ActivityArginineHplcPlant ExtractsQuantitationhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1Huperzia saururus (Lam.) Trevis. (Lycopodiaceae), an autochthonous plant species in Argentina, is used as a memory improver in traditional medicine. It was studied for this reason and the purified alkaloid extract did show significant activity on learning and memory. The species is mostly consumed in the form of infusions and decoctions. Objectives: To evaluate the activity of the H. saururus infusion and decoction as inhibitors of acetylcholinesterase (AChE) and to determine the amino acid content in both extracts. Material and methods: Infusion and decoction were purified by ionic exchange chromatography and analyzed by high-performance liquid chromatography HPLC-UV, and the AChE inhibition of these extracts was evaluated by using the Ellman method. Results: Both infusion and decoction exerted strong AChE inhibitory activities (IC50 ¼ 7.2 0.4 and 22.7 5.6 mg/mL, respectively). Among nine amino acids, arginine (Arg) was identified in a concentration greater than 9 mg/100 g of dried aerial parts in both extracts. Discussion and conclusion: This high content of Arg could be considered a contributing factor to the activity on memory previously demonstrated for the H. saururus alkaloid extract, since Arg is implicated indirectly in mnemonic processes as a precursor in nitric oxide synthesis. Thus, the central effect of H. saururus could involve two different mechanisms, the cholinergic mechanism and the nitric oxide pathway.Fil: Vallejo, Mariana Guadalupe. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); ArgentinaFil: Dimmer, Jesica Ayelen. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); ArgentinaFil: Ortega, María Gabriela. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); ArgentinaFil: Cabrera, Jose Luis. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); ArgentinaFil: Agnese, Alicia Mariel. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); ArgentinaTaylor & Francis2013-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/9317Vallejo, Mariana Guadalupe; Dimmer, Jesica Ayelen; Ortega, María Gabriela; Cabrera, Jose Luis; Agnese, Alicia Mariel; Amino acid content and acetylcholinesterase inhibition of Huperzia saururus infusion and decoction; Taylor & Francis; Pharmaceutical Biology; 51; 9; 7-2013; 1341-13451388-02091744-5116enginfo:eu-repo/semantics/altIdentifier/doi/10.3109/13880209.2013.784921info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.3109/13880209.2013.784921info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-12-23T14:05:03Zoai:ri.conicet.gov.ar:11336/9317instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-12-23 14:05:03.509CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Amino acid content and acetylcholinesterase inhibition of Huperzia saururus infusion and decoction
title Amino acid content and acetylcholinesterase inhibition of Huperzia saururus infusion and decoction
spellingShingle Amino acid content and acetylcholinesterase inhibition of Huperzia saururus infusion and decoction
Vallejo, Mariana Guadalupe
Anti-Cholinesterase Activity
Arginine
Hplc
Plant Extracts
Quantitation
title_short Amino acid content and acetylcholinesterase inhibition of Huperzia saururus infusion and decoction
title_full Amino acid content and acetylcholinesterase inhibition of Huperzia saururus infusion and decoction
title_fullStr Amino acid content and acetylcholinesterase inhibition of Huperzia saururus infusion and decoction
title_full_unstemmed Amino acid content and acetylcholinesterase inhibition of Huperzia saururus infusion and decoction
title_sort Amino acid content and acetylcholinesterase inhibition of Huperzia saururus infusion and decoction
dc.creator.none.fl_str_mv Vallejo, Mariana Guadalupe
Dimmer, Jesica Ayelen
Ortega, María Gabriela
Cabrera, Jose Luis
Agnese, Alicia Mariel
author Vallejo, Mariana Guadalupe
author_facet Vallejo, Mariana Guadalupe
Dimmer, Jesica Ayelen
Ortega, María Gabriela
Cabrera, Jose Luis
Agnese, Alicia Mariel
author_role author
author2 Dimmer, Jesica Ayelen
Ortega, María Gabriela
Cabrera, Jose Luis
Agnese, Alicia Mariel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Anti-Cholinesterase Activity
Arginine
Hplc
Plant Extracts
Quantitation
topic Anti-Cholinesterase Activity
Arginine
Hplc
Plant Extracts
Quantitation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Huperzia saururus (Lam.) Trevis. (Lycopodiaceae), an autochthonous plant species in Argentina, is used as a memory improver in traditional medicine. It was studied for this reason and the purified alkaloid extract did show significant activity on learning and memory. The species is mostly consumed in the form of infusions and decoctions. Objectives: To evaluate the activity of the H. saururus infusion and decoction as inhibitors of acetylcholinesterase (AChE) and to determine the amino acid content in both extracts. Material and methods: Infusion and decoction were purified by ionic exchange chromatography and analyzed by high-performance liquid chromatography HPLC-UV, and the AChE inhibition of these extracts was evaluated by using the Ellman method. Results: Both infusion and decoction exerted strong AChE inhibitory activities (IC50 ¼ 7.2 0.4 and 22.7 5.6 mg/mL, respectively). Among nine amino acids, arginine (Arg) was identified in a concentration greater than 9 mg/100 g of dried aerial parts in both extracts. Discussion and conclusion: This high content of Arg could be considered a contributing factor to the activity on memory previously demonstrated for the H. saururus alkaloid extract, since Arg is implicated indirectly in mnemonic processes as a precursor in nitric oxide synthesis. Thus, the central effect of H. saururus could involve two different mechanisms, the cholinergic mechanism and the nitric oxide pathway.
Fil: Vallejo, Mariana Guadalupe. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina
Fil: Dimmer, Jesica Ayelen. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina
Fil: Ortega, María Gabriela. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina
Fil: Cabrera, Jose Luis. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina
Fil: Agnese, Alicia Mariel. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Instituto Multidisciplinario de Biología Vegetal (p); Argentina
description Huperzia saururus (Lam.) Trevis. (Lycopodiaceae), an autochthonous plant species in Argentina, is used as a memory improver in traditional medicine. It was studied for this reason and the purified alkaloid extract did show significant activity on learning and memory. The species is mostly consumed in the form of infusions and decoctions. Objectives: To evaluate the activity of the H. saururus infusion and decoction as inhibitors of acetylcholinesterase (AChE) and to determine the amino acid content in both extracts. Material and methods: Infusion and decoction were purified by ionic exchange chromatography and analyzed by high-performance liquid chromatography HPLC-UV, and the AChE inhibition of these extracts was evaluated by using the Ellman method. Results: Both infusion and decoction exerted strong AChE inhibitory activities (IC50 ¼ 7.2 0.4 and 22.7 5.6 mg/mL, respectively). Among nine amino acids, arginine (Arg) was identified in a concentration greater than 9 mg/100 g of dried aerial parts in both extracts. Discussion and conclusion: This high content of Arg could be considered a contributing factor to the activity on memory previously demonstrated for the H. saururus alkaloid extract, since Arg is implicated indirectly in mnemonic processes as a precursor in nitric oxide synthesis. Thus, the central effect of H. saururus could involve two different mechanisms, the cholinergic mechanism and the nitric oxide pathway.
publishDate 2013
dc.date.none.fl_str_mv 2013-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/9317
Vallejo, Mariana Guadalupe; Dimmer, Jesica Ayelen; Ortega, María Gabriela; Cabrera, Jose Luis; Agnese, Alicia Mariel; Amino acid content and acetylcholinesterase inhibition of Huperzia saururus infusion and decoction; Taylor & Francis; Pharmaceutical Biology; 51; 9; 7-2013; 1341-1345
1388-0209
1744-5116
url http://hdl.handle.net/11336/9317
identifier_str_mv Vallejo, Mariana Guadalupe; Dimmer, Jesica Ayelen; Ortega, María Gabriela; Cabrera, Jose Luis; Agnese, Alicia Mariel; Amino acid content and acetylcholinesterase inhibition of Huperzia saururus infusion and decoction; Taylor & Francis; Pharmaceutical Biology; 51; 9; 7-2013; 1341-1345
1388-0209
1744-5116
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3109/13880209.2013.784921
info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.3109/13880209.2013.784921
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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