Huperzia quadrifariata and Huperzia reflexa alkaloids inhibit acetylcholinesterase activity in vivo in mice brain

Autores
Konrath, Eduardo Luis; Neves, Bruna Medeiros; Passos, Carolina Dos Santos; Lunardi, Paula Santana; Ortega, María Gabriela; Cabrera, Jose Luis; Goncalves, C. A.; Henriques, Amalia Teresinha
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Huperzine A, a Lycopodium alkaloid produced by Chinese folk herb Huperzia serrata (Lycopodiaceae), has been shown to be a promising agent for the treatment of Alzheimer's disease due to its potent acetylcholinesterase (AChE) activity, as well its efficacy in the treatment of memory of aged patients. Thus, the effects of two Huperzia species of habitats in Brazil (H. quadrifariata and H. reflexa) with described in vitro AChE inhibition activities were studied and their effects on mice brain AChE inhibition were determined after a single intraperitoneal (i.p.) injection. The alkaloid extracts were administered to mice in various doses (10, 1 and 0.5 mg/kg) and acetylcholinesterase activity was measured post mortem in two brain areas using the Ellman's colorimetric method. The AChE activity was found to be significantly reduced in both the cortex and hippocampus, although this activity was less potent than that of reference inhibitor huperzine A (0.5 mg/kg). Thus, it appears that H. quadrifariata and H. reflexa alkaloid extracts, shown to inhibit acetylcholinesterase in vitro, also have very potent in vivo effects, suggesting that the Huperzia species may still constitute a promising source of compounds with pharmaceutical interest for Alzheimer's disease.
Fil: Konrath, Eduardo Luis. Universidade Federal do Rio Grande do Sul. Programa de Pós Graduação em Ciências Farmacêuticas; Brasil
Fil: Neves, Bruna Medeiros. Universidade Federal do Rio Grande do Sul. Programa de Pós Graduação em Ciências Farmacêuticas; Brasil
Fil: Passos, Carolina Dos Santos. Universidade Federal do Rio Grande do Sul. Programa de Pós Graduação em Ciências Farmacêuticas; Brasil
Fil: Lunardi, Paula Santana. Universidade Federal do Rio Grande do Sul. Departamento de Bioquímica; Brasil
Fil: Ortega, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia. Catedra de Farmacognosia; Argentina
Fil: Cabrera, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia. Catedra de Farmacognosia; Argentina
Fil: Goncalves, C. A.. Universidade Federal do Rio Grande do Sul. Departamento de Bioquímica; Brasil
Fil: Henriques, Amalia Teresinha. Universidade Federal do Rio Grande do Sul. Programa de Pós Graduação em Ciências Farmacêuticas; Brasil
Materia
Huperzia Quadrifariata
Huperzia Reflexa
Lycopodiaceae
Acetylcholinesterase Activity
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/18853
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/18853
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Huperzia quadrifariata and Huperzia reflexa alkaloids inhibit acetylcholinesterase activity in vivo in mice brainKonrath, Eduardo LuisNeves, Bruna MedeirosPassos, Carolina Dos SantosLunardi, Paula SantanaOrtega, María GabrielaCabrera, Jose LuisGoncalves, C. A.Henriques, Amalia TeresinhaHuperzia QuadrifariataHuperzia ReflexaLycopodiaceaeAcetylcholinesterase Activityhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Huperzine A, a Lycopodium alkaloid produced by Chinese folk herb Huperzia serrata (Lycopodiaceae), has been shown to be a promising agent for the treatment of Alzheimer's disease due to its potent acetylcholinesterase (AChE) activity, as well its efficacy in the treatment of memory of aged patients. Thus, the effects of two Huperzia species of habitats in Brazil (H. quadrifariata and H. reflexa) with described in vitro AChE inhibition activities were studied and their effects on mice brain AChE inhibition were determined after a single intraperitoneal (i.p.) injection. The alkaloid extracts were administered to mice in various doses (10, 1 and 0.5 mg/kg) and acetylcholinesterase activity was measured post mortem in two brain areas using the Ellman's colorimetric method. The AChE activity was found to be significantly reduced in both the cortex and hippocampus, although this activity was less potent than that of reference inhibitor huperzine A (0.5 mg/kg). Thus, it appears that H. quadrifariata and H. reflexa alkaloid extracts, shown to inhibit acetylcholinesterase in vitro, also have very potent in vivo effects, suggesting that the Huperzia species may still constitute a promising source of compounds with pharmaceutical interest for Alzheimer's disease.Fil: Konrath, Eduardo Luis. Universidade Federal do Rio Grande do Sul. Programa de Pós Graduação em Ciências Farmacêuticas; BrasilFil: Neves, Bruna Medeiros. Universidade Federal do Rio Grande do Sul. Programa de Pós Graduação em Ciências Farmacêuticas; BrasilFil: Passos, Carolina Dos Santos. Universidade Federal do Rio Grande do Sul. Programa de Pós Graduação em Ciências Farmacêuticas; BrasilFil: Lunardi, Paula Santana. Universidade Federal do Rio Grande do Sul. Departamento de Bioquímica; BrasilFil: Ortega, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia. Catedra de Farmacognosia; ArgentinaFil: Cabrera, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia. Catedra de Farmacognosia; ArgentinaFil: Goncalves, C. A.. Universidade Federal do Rio Grande do Sul. Departamento de Bioquímica; BrasilFil: Henriques, Amalia Teresinha. Universidade Federal do Rio Grande do Sul. Programa de Pós Graduação em Ciências Farmacêuticas; BrasilElsevier Gmbh2012-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/18853Konrath, Eduardo Luis; Neves, Bruna Medeiros; Passos, Carolina Dos Santos; Lunardi, Paula Santana; Ortega, María Gabriela; et al.; Huperzia quadrifariata and Huperzia reflexa alkaloids inhibit acetylcholinesterase activity in vivo in mice brain; Elsevier Gmbh; Phytomedicine; 19; 14; 11-2012; 1321-13240944-71131618-095XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0944711312002838info:eu-repo/semantics/altIdentifier/doi/10.1016/j.phymed.2012.08.009info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:24:56Zoai:ri.conicet.gov.ar:11336/18853instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:24:56.296CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Huperzia quadrifariata and Huperzia reflexa alkaloids inhibit acetylcholinesterase activity in vivo in mice brain
title Huperzia quadrifariata and Huperzia reflexa alkaloids inhibit acetylcholinesterase activity in vivo in mice brain
spellingShingle Huperzia quadrifariata and Huperzia reflexa alkaloids inhibit acetylcholinesterase activity in vivo in mice brain
Konrath, Eduardo Luis
Huperzia Quadrifariata
Huperzia Reflexa
Lycopodiaceae
Acetylcholinesterase Activity
title_short Huperzia quadrifariata and Huperzia reflexa alkaloids inhibit acetylcholinesterase activity in vivo in mice brain
title_full Huperzia quadrifariata and Huperzia reflexa alkaloids inhibit acetylcholinesterase activity in vivo in mice brain
title_fullStr Huperzia quadrifariata and Huperzia reflexa alkaloids inhibit acetylcholinesterase activity in vivo in mice brain
title_full_unstemmed Huperzia quadrifariata and Huperzia reflexa alkaloids inhibit acetylcholinesterase activity in vivo in mice brain
title_sort Huperzia quadrifariata and Huperzia reflexa alkaloids inhibit acetylcholinesterase activity in vivo in mice brain
dc.creator.none.fl_str_mv Konrath, Eduardo Luis
Neves, Bruna Medeiros
Passos, Carolina Dos Santos
Lunardi, Paula Santana
Ortega, María Gabriela
Cabrera, Jose Luis
Goncalves, C. A.
Henriques, Amalia Teresinha
author Konrath, Eduardo Luis
author_facet Konrath, Eduardo Luis
Neves, Bruna Medeiros
Passos, Carolina Dos Santos
Lunardi, Paula Santana
Ortega, María Gabriela
Cabrera, Jose Luis
Goncalves, C. A.
Henriques, Amalia Teresinha
author_role author
author2 Neves, Bruna Medeiros
Passos, Carolina Dos Santos
Lunardi, Paula Santana
Ortega, María Gabriela
Cabrera, Jose Luis
Goncalves, C. A.
Henriques, Amalia Teresinha
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Huperzia Quadrifariata
Huperzia Reflexa
Lycopodiaceae
Acetylcholinesterase Activity
topic Huperzia Quadrifariata
Huperzia Reflexa
Lycopodiaceae
Acetylcholinesterase Activity
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Huperzine A, a Lycopodium alkaloid produced by Chinese folk herb Huperzia serrata (Lycopodiaceae), has been shown to be a promising agent for the treatment of Alzheimer's disease due to its potent acetylcholinesterase (AChE) activity, as well its efficacy in the treatment of memory of aged patients. Thus, the effects of two Huperzia species of habitats in Brazil (H. quadrifariata and H. reflexa) with described in vitro AChE inhibition activities were studied and their effects on mice brain AChE inhibition were determined after a single intraperitoneal (i.p.) injection. The alkaloid extracts were administered to mice in various doses (10, 1 and 0.5 mg/kg) and acetylcholinesterase activity was measured post mortem in two brain areas using the Ellman's colorimetric method. The AChE activity was found to be significantly reduced in both the cortex and hippocampus, although this activity was less potent than that of reference inhibitor huperzine A (0.5 mg/kg). Thus, it appears that H. quadrifariata and H. reflexa alkaloid extracts, shown to inhibit acetylcholinesterase in vitro, also have very potent in vivo effects, suggesting that the Huperzia species may still constitute a promising source of compounds with pharmaceutical interest for Alzheimer's disease.
Fil: Konrath, Eduardo Luis. Universidade Federal do Rio Grande do Sul. Programa de Pós Graduação em Ciências Farmacêuticas; Brasil
Fil: Neves, Bruna Medeiros. Universidade Federal do Rio Grande do Sul. Programa de Pós Graduação em Ciências Farmacêuticas; Brasil
Fil: Passos, Carolina Dos Santos. Universidade Federal do Rio Grande do Sul. Programa de Pós Graduação em Ciências Farmacêuticas; Brasil
Fil: Lunardi, Paula Santana. Universidade Federal do Rio Grande do Sul. Departamento de Bioquímica; Brasil
Fil: Ortega, María Gabriela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia. Catedra de Farmacognosia; Argentina
Fil: Cabrera, Jose Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto Multidisciplinario de Biología Vegetal. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Instituto Multidisciplinario de Biología Vegetal; Argentina. Universidad Nacional de Cordoba. Facultad de Ciencias Quimicas. Departamento de Farmacia. Catedra de Farmacognosia; Argentina
Fil: Goncalves, C. A.. Universidade Federal do Rio Grande do Sul. Departamento de Bioquímica; Brasil
Fil: Henriques, Amalia Teresinha. Universidade Federal do Rio Grande do Sul. Programa de Pós Graduação em Ciências Farmacêuticas; Brasil
description Huperzine A, a Lycopodium alkaloid produced by Chinese folk herb Huperzia serrata (Lycopodiaceae), has been shown to be a promising agent for the treatment of Alzheimer's disease due to its potent acetylcholinesterase (AChE) activity, as well its efficacy in the treatment of memory of aged patients. Thus, the effects of two Huperzia species of habitats in Brazil (H. quadrifariata and H. reflexa) with described in vitro AChE inhibition activities were studied and their effects on mice brain AChE inhibition were determined after a single intraperitoneal (i.p.) injection. The alkaloid extracts were administered to mice in various doses (10, 1 and 0.5 mg/kg) and acetylcholinesterase activity was measured post mortem in two brain areas using the Ellman's colorimetric method. The AChE activity was found to be significantly reduced in both the cortex and hippocampus, although this activity was less potent than that of reference inhibitor huperzine A (0.5 mg/kg). Thus, it appears that H. quadrifariata and H. reflexa alkaloid extracts, shown to inhibit acetylcholinesterase in vitro, also have very potent in vivo effects, suggesting that the Huperzia species may still constitute a promising source of compounds with pharmaceutical interest for Alzheimer's disease.
publishDate 2012
dc.date.none.fl_str_mv 2012-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/18853
Konrath, Eduardo Luis; Neves, Bruna Medeiros; Passos, Carolina Dos Santos; Lunardi, Paula Santana; Ortega, María Gabriela; et al.; Huperzia quadrifariata and Huperzia reflexa alkaloids inhibit acetylcholinesterase activity in vivo in mice brain; Elsevier Gmbh; Phytomedicine; 19; 14; 11-2012; 1321-1324
0944-7113
1618-095X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/18853
identifier_str_mv Konrath, Eduardo Luis; Neves, Bruna Medeiros; Passos, Carolina Dos Santos; Lunardi, Paula Santana; Ortega, María Gabriela; et al.; Huperzia quadrifariata and Huperzia reflexa alkaloids inhibit acetylcholinesterase activity in vivo in mice brain; Elsevier Gmbh; Phytomedicine; 19; 14; 11-2012; 1321-1324
0944-7113
1618-095X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0944711312002838
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.phymed.2012.08.009
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Gmbh
publisher.none.fl_str_mv Elsevier Gmbh
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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