The corn influence on the adsorption levels of aflatoxin B1 and zearalenone by yeast cell wall

Autores
Pereyra, Carina Maricel; Cavaglieri, Lilia Reneé; Chiacchiera, Stella Maris; Dalcero, Ana Maria
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Aims: To in vitro evaluate the influence of the corn on the adsorption levels of aflatoxin B1 (AFB1) and zearalenone (ZEA) by yeast cell walls (YCWs). Methods and Results: Two commercial YCWs were studied. The YCWs contain different percentages of polysaccharides. YCW1 and 2 contain 5·9 and 21% of mannans and 17·4 and 23% of β-glucans, respectively. Each YCW was resuspended in pH 2 and pH 6 buffer solutions. Corn was used to study the matrix influence. An aliquot of 500 μl YCW suspension was added to each microtube containing 500 μl of 0·1, 0·25, 0·5, 1, 2·5 and 5 μg ml−1 AFB1 or 0·5, 5, 10, 20 and 50 μg ml−1 ZEA. Microtubes were kept with mechanical agitation at 37°C for 30 min and then centrifuged for 10 min at 16 873 g and; the supernatants were quantified by high-pressure liquid chromatography. The amount of bound toxin was plotted as a function of the amount of added toxin according to mathematical expressions proposed by three theoretical models. Both YCWs were capable of adsorbing AFB1 and ZEA in amounts from 0·061 to 0·40 and from 0·10 and 0·26 g g−1, respectively. In the presence of the matrix, both adsorbents were not able to adsorb AFB1. However, they could adsorb ZEA at levels from 0·03 to 0·23 g g−1. Conclusions: Both YCWs adsorbed ZEA in the presence of corn and also under simulated gastrointestinal pH conditions. These results suggest that the studied YCWs are potential candidates for ZEA adsorption. Significance and Impact of the Study: Several in vitro assays have informed the ability of different substrates including yeast walls to adsorb AFB1 and ZEA; none of them have evaluated their ability to adsorb AFB1 and ZEA in the presence of the corn. The corn matrix can influence the adsorption phenomena of these mycotoxins.
Fil: Pereyra, Carina Maricel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chiacchiera, Stella Maris. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dalcero, Ana Maria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Matrix
Adsorption
Aflatoxins
Zearalenone
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/24683
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/24683
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling The corn influence on the adsorption levels of aflatoxin B1 and zearalenone by yeast cell wallPereyra, Carina MaricelCavaglieri, Lilia ReneéChiacchiera, Stella MarisDalcero, Ana MariaMatrixAdsorptionAflatoxinsZearalenonehttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Aims: To in vitro evaluate the influence of the corn on the adsorption levels of aflatoxin B1 (AFB1) and zearalenone (ZEA) by yeast cell walls (YCWs). Methods and Results: Two commercial YCWs were studied. The YCWs contain different percentages of polysaccharides. YCW1 and 2 contain 5·9 and 21% of mannans and 17·4 and 23% of β-glucans, respectively. Each YCW was resuspended in pH 2 and pH 6 buffer solutions. Corn was used to study the matrix influence. An aliquot of 500 μl YCW suspension was added to each microtube containing 500 μl of 0·1, 0·25, 0·5, 1, 2·5 and 5 μg ml−1 AFB1 or 0·5, 5, 10, 20 and 50 μg ml−1 ZEA. Microtubes were kept with mechanical agitation at 37°C for 30 min and then centrifuged for 10 min at 16 873 g and; the supernatants were quantified by high-pressure liquid chromatography. The amount of bound toxin was plotted as a function of the amount of added toxin according to mathematical expressions proposed by three theoretical models. Both YCWs were capable of adsorbing AFB1 and ZEA in amounts from 0·061 to 0·40 and from 0·10 and 0·26 g g−1, respectively. In the presence of the matrix, both adsorbents were not able to adsorb AFB1. However, they could adsorb ZEA at levels from 0·03 to 0·23 g g−1. Conclusions: Both YCWs adsorbed ZEA in the presence of corn and also under simulated gastrointestinal pH conditions. These results suggest that the studied YCWs are potential candidates for ZEA adsorption. Significance and Impact of the Study: Several in vitro assays have informed the ability of different substrates including yeast walls to adsorb AFB1 and ZEA; none of them have evaluated their ability to adsorb AFB1 and ZEA in the presence of the corn. The corn matrix can influence the adsorption phenomena of these mycotoxins.Fil: Pereyra, Carina Maricel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Chiacchiera, Stella Maris. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Dalcero, Ana Maria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaWiley2013-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/24683Pereyra, Carina Maricel; Cavaglieri, Lilia Reneé; Chiacchiera, Stella Maris; Dalcero, Ana Maria; The corn influence on the adsorption levels of aflatoxin B1 and zearalenone by yeast cell wall; Wiley; Journal of Applied Microbiology; 114; 3; 1-2013; 655-6621364-5072CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/jam.12082info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jam.12082/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:08:42Zoai:ri.conicet.gov.ar:11336/24683instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:08:42.756CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv The corn influence on the adsorption levels of aflatoxin B1 and zearalenone by yeast cell wall
title The corn influence on the adsorption levels of aflatoxin B1 and zearalenone by yeast cell wall
spellingShingle The corn influence on the adsorption levels of aflatoxin B1 and zearalenone by yeast cell wall
Pereyra, Carina Maricel
Matrix
Adsorption
Aflatoxins
Zearalenone
title_short The corn influence on the adsorption levels of aflatoxin B1 and zearalenone by yeast cell wall
title_full The corn influence on the adsorption levels of aflatoxin B1 and zearalenone by yeast cell wall
title_fullStr The corn influence on the adsorption levels of aflatoxin B1 and zearalenone by yeast cell wall
title_full_unstemmed The corn influence on the adsorption levels of aflatoxin B1 and zearalenone by yeast cell wall
title_sort The corn influence on the adsorption levels of aflatoxin B1 and zearalenone by yeast cell wall
dc.creator.none.fl_str_mv Pereyra, Carina Maricel
Cavaglieri, Lilia Reneé
Chiacchiera, Stella Maris
Dalcero, Ana Maria
author Pereyra, Carina Maricel
author_facet Pereyra, Carina Maricel
Cavaglieri, Lilia Reneé
Chiacchiera, Stella Maris
Dalcero, Ana Maria
author_role author
author2 Cavaglieri, Lilia Reneé
Chiacchiera, Stella Maris
Dalcero, Ana Maria
author2_role author
author
author
dc.subject.none.fl_str_mv Matrix
Adsorption
Aflatoxins
Zearalenone
topic Matrix
Adsorption
Aflatoxins
Zearalenone
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Aims: To in vitro evaluate the influence of the corn on the adsorption levels of aflatoxin B1 (AFB1) and zearalenone (ZEA) by yeast cell walls (YCWs). Methods and Results: Two commercial YCWs were studied. The YCWs contain different percentages of polysaccharides. YCW1 and 2 contain 5·9 and 21% of mannans and 17·4 and 23% of β-glucans, respectively. Each YCW was resuspended in pH 2 and pH 6 buffer solutions. Corn was used to study the matrix influence. An aliquot of 500 μl YCW suspension was added to each microtube containing 500 μl of 0·1, 0·25, 0·5, 1, 2·5 and 5 μg ml−1 AFB1 or 0·5, 5, 10, 20 and 50 μg ml−1 ZEA. Microtubes were kept with mechanical agitation at 37°C for 30 min and then centrifuged for 10 min at 16 873 g and; the supernatants were quantified by high-pressure liquid chromatography. The amount of bound toxin was plotted as a function of the amount of added toxin according to mathematical expressions proposed by three theoretical models. Both YCWs were capable of adsorbing AFB1 and ZEA in amounts from 0·061 to 0·40 and from 0·10 and 0·26 g g−1, respectively. In the presence of the matrix, both adsorbents were not able to adsorb AFB1. However, they could adsorb ZEA at levels from 0·03 to 0·23 g g−1. Conclusions: Both YCWs adsorbed ZEA in the presence of corn and also under simulated gastrointestinal pH conditions. These results suggest that the studied YCWs are potential candidates for ZEA adsorption. Significance and Impact of the Study: Several in vitro assays have informed the ability of different substrates including yeast walls to adsorb AFB1 and ZEA; none of them have evaluated their ability to adsorb AFB1 and ZEA in the presence of the corn. The corn matrix can influence the adsorption phenomena of these mycotoxins.
Fil: Pereyra, Carina Maricel. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cavaglieri, Lilia Reneé. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chiacchiera, Stella Maris. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas Fisicoquímicas y Naturales. Departamento de Química; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Dalcero, Ana Maria. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Aims: To in vitro evaluate the influence of the corn on the adsorption levels of aflatoxin B1 (AFB1) and zearalenone (ZEA) by yeast cell walls (YCWs). Methods and Results: Two commercial YCWs were studied. The YCWs contain different percentages of polysaccharides. YCW1 and 2 contain 5·9 and 21% of mannans and 17·4 and 23% of β-glucans, respectively. Each YCW was resuspended in pH 2 and pH 6 buffer solutions. Corn was used to study the matrix influence. An aliquot of 500 μl YCW suspension was added to each microtube containing 500 μl of 0·1, 0·25, 0·5, 1, 2·5 and 5 μg ml−1 AFB1 or 0·5, 5, 10, 20 and 50 μg ml−1 ZEA. Microtubes were kept with mechanical agitation at 37°C for 30 min and then centrifuged for 10 min at 16 873 g and; the supernatants were quantified by high-pressure liquid chromatography. The amount of bound toxin was plotted as a function of the amount of added toxin according to mathematical expressions proposed by three theoretical models. Both YCWs were capable of adsorbing AFB1 and ZEA in amounts from 0·061 to 0·40 and from 0·10 and 0·26 g g−1, respectively. In the presence of the matrix, both adsorbents were not able to adsorb AFB1. However, they could adsorb ZEA at levels from 0·03 to 0·23 g g−1. Conclusions: Both YCWs adsorbed ZEA in the presence of corn and also under simulated gastrointestinal pH conditions. These results suggest that the studied YCWs are potential candidates for ZEA adsorption. Significance and Impact of the Study: Several in vitro assays have informed the ability of different substrates including yeast walls to adsorb AFB1 and ZEA; none of them have evaluated their ability to adsorb AFB1 and ZEA in the presence of the corn. The corn matrix can influence the adsorption phenomena of these mycotoxins.
publishDate 2013
dc.date.none.fl_str_mv 2013-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/24683
Pereyra, Carina Maricel; Cavaglieri, Lilia Reneé; Chiacchiera, Stella Maris; Dalcero, Ana Maria; The corn influence on the adsorption levels of aflatoxin B1 and zearalenone by yeast cell wall; Wiley; Journal of Applied Microbiology; 114; 3; 1-2013; 655-662
1364-5072
CONICET Digital
CONICET
url http://hdl.handle.net/11336/24683
identifier_str_mv Pereyra, Carina Maricel; Cavaglieri, Lilia Reneé; Chiacchiera, Stella Maris; Dalcero, Ana Maria; The corn influence on the adsorption levels of aflatoxin B1 and zearalenone by yeast cell wall; Wiley; Journal of Applied Microbiology; 114; 3; 1-2013; 655-662
1364-5072
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/jam.12082
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/jam.12082/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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