Cloning, Expression, Purification And Physical And Kinetic Characterization Of The Phosphoenolpyruvate Carboxylase From Orange (Citrus sinensis Osbeck var. Valencia) Fruit Juice Sa...

Autores
Perotti, Valeria Elisa; Figueroa, Carlos Maria; Andreo, Carlos Santiago; Iglesias, Alberto Alvaro; Podesta, Florencio Esteban
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Phosphoenolpyruvate (PEP) carboxylase (PEPCase) from orange fruit juice sacs has been cloned and heterogously expressed in high yield. The purified recombinant enzyme displays properties typical of plant PEPCase, including activation by sugar phosphates and inhibition by malate and citrate. Malate inhibition is weak in the physiological pH range, and the enzyme is also poorly affected by Glu and Asp, known inhibitors of C3 plants PEPCases. However, it is strongly inhibited by citrate. Orange fruit PEPCase phosphorylation by mammalian protein kinase A decreased inhibition by malate. The enzyme presents an unusual high molecular mass in the absence of PEP, while in its presence it displays a more common tetrameric arrangement. The overall properties of the enzyme suggest that it is suited for organic acid synthesis and NADH reoxidation in the mature fruit. The present study provides the first analysis of a recombinant fruit PEPCase.
Fil: Perotti, Valeria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Figueroa, Carlos Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Andreo, Carlos Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Materia
CITRUS SINENSIS
ORANGE FRUIT
PHOSPHOENOLPYRUVATE CARBOXYLASE
CARBON METABOLISM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/84777
Acceder
id CONICETDig_d8bdcde34f7d1763a142fb6db5967363
oai_identifier_str oai:ri.conicet.gov.ar:11336/84777
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Cloning, Expression, Purification And Physical And Kinetic Characterization Of The Phosphoenolpyruvate Carboxylase From Orange (Citrus sinensis Osbeck var. Valencia) Fruit Juice SacsPerotti, Valeria ElisaFigueroa, Carlos MariaAndreo, Carlos SantiagoIglesias, Alberto AlvaroPodesta, Florencio EstebanCITRUS SINENSISORANGE FRUITPHOSPHOENOLPYRUVATE CARBOXYLASECARBON METABOLISMhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Phosphoenolpyruvate (PEP) carboxylase (PEPCase) from orange fruit juice sacs has been cloned and heterogously expressed in high yield. The purified recombinant enzyme displays properties typical of plant PEPCase, including activation by sugar phosphates and inhibition by malate and citrate. Malate inhibition is weak in the physiological pH range, and the enzyme is also poorly affected by Glu and Asp, known inhibitors of C3 plants PEPCases. However, it is strongly inhibited by citrate. Orange fruit PEPCase phosphorylation by mammalian protein kinase A decreased inhibition by malate. The enzyme presents an unusual high molecular mass in the absence of PEP, while in its presence it displays a more common tetrameric arrangement. The overall properties of the enzyme suggest that it is suited for organic acid synthesis and NADH reoxidation in the mature fruit. The present study provides the first analysis of a recombinant fruit PEPCase.Fil: Perotti, Valeria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Figueroa, Carlos Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Andreo, Carlos Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaFil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; ArgentinaFil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; ArgentinaElsevier2010-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/84777Perotti, Valeria Elisa; Figueroa, Carlos Maria; Andreo, Carlos Santiago; Iglesias, Alberto Alvaro; Podesta, Florencio Esteban; Cloning, Expression, Purification And Physical And Kinetic Characterization Of The Phosphoenolpyruvate Carboxylase From Orange (Citrus sinensis Osbeck var. Valencia) Fruit Juice Sacs; Elsevier; Plant Science; 179; 5; 11-2010; 527-5350168-9452CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.plantsci.2010.08.003info:eu-repo/semantics/altIdentifier/url/https://pubag.nal.usda.gov/catalog/792389info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:05:17Zoai:ri.conicet.gov.ar:11336/84777instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:05:17.449CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Cloning, Expression, Purification And Physical And Kinetic Characterization Of The Phosphoenolpyruvate Carboxylase From Orange (Citrus sinensis Osbeck var. Valencia) Fruit Juice Sacs
title Cloning, Expression, Purification And Physical And Kinetic Characterization Of The Phosphoenolpyruvate Carboxylase From Orange (Citrus sinensis Osbeck var. Valencia) Fruit Juice Sacs
spellingShingle Cloning, Expression, Purification And Physical And Kinetic Characterization Of The Phosphoenolpyruvate Carboxylase From Orange (Citrus sinensis Osbeck var. Valencia) Fruit Juice Sacs
Perotti, Valeria Elisa
CITRUS SINENSIS
ORANGE FRUIT
PHOSPHOENOLPYRUVATE CARBOXYLASE
CARBON METABOLISM
title_short Cloning, Expression, Purification And Physical And Kinetic Characterization Of The Phosphoenolpyruvate Carboxylase From Orange (Citrus sinensis Osbeck var. Valencia) Fruit Juice Sacs
title_full Cloning, Expression, Purification And Physical And Kinetic Characterization Of The Phosphoenolpyruvate Carboxylase From Orange (Citrus sinensis Osbeck var. Valencia) Fruit Juice Sacs
title_fullStr Cloning, Expression, Purification And Physical And Kinetic Characterization Of The Phosphoenolpyruvate Carboxylase From Orange (Citrus sinensis Osbeck var. Valencia) Fruit Juice Sacs
title_full_unstemmed Cloning, Expression, Purification And Physical And Kinetic Characterization Of The Phosphoenolpyruvate Carboxylase From Orange (Citrus sinensis Osbeck var. Valencia) Fruit Juice Sacs
title_sort Cloning, Expression, Purification And Physical And Kinetic Characterization Of The Phosphoenolpyruvate Carboxylase From Orange (Citrus sinensis Osbeck var. Valencia) Fruit Juice Sacs
dc.creator.none.fl_str_mv Perotti, Valeria Elisa
Figueroa, Carlos Maria
Andreo, Carlos Santiago
Iglesias, Alberto Alvaro
Podesta, Florencio Esteban
author Perotti, Valeria Elisa
author_facet Perotti, Valeria Elisa
Figueroa, Carlos Maria
Andreo, Carlos Santiago
Iglesias, Alberto Alvaro
Podesta, Florencio Esteban
author_role author
author2 Figueroa, Carlos Maria
Andreo, Carlos Santiago
Iglesias, Alberto Alvaro
Podesta, Florencio Esteban
author2_role author
author
author
author
dc.subject.none.fl_str_mv CITRUS SINENSIS
ORANGE FRUIT
PHOSPHOENOLPYRUVATE CARBOXYLASE
CARBON METABOLISM
topic CITRUS SINENSIS
ORANGE FRUIT
PHOSPHOENOLPYRUVATE CARBOXYLASE
CARBON METABOLISM
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Phosphoenolpyruvate (PEP) carboxylase (PEPCase) from orange fruit juice sacs has been cloned and heterogously expressed in high yield. The purified recombinant enzyme displays properties typical of plant PEPCase, including activation by sugar phosphates and inhibition by malate and citrate. Malate inhibition is weak in the physiological pH range, and the enzyme is also poorly affected by Glu and Asp, known inhibitors of C3 plants PEPCases. However, it is strongly inhibited by citrate. Orange fruit PEPCase phosphorylation by mammalian protein kinase A decreased inhibition by malate. The enzyme presents an unusual high molecular mass in the absence of PEP, while in its presence it displays a more common tetrameric arrangement. The overall properties of the enzyme suggest that it is suited for organic acid synthesis and NADH reoxidation in the mature fruit. The present study provides the first analysis of a recombinant fruit PEPCase.
Fil: Perotti, Valeria Elisa. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Figueroa, Carlos Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Andreo, Carlos Santiago. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
Fil: Iglesias, Alberto Alvaro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Agrobiotecnología del Litoral. Universidad Nacional del Litoral. Instituto de Agrobiotecnología del Litoral; Argentina
Fil: Podesta, Florencio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Centro de Estudios Fotosintéticos y Bioquímicos. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Centro de Estudios Fotosintéticos y Bioquímicos; Argentina
description Phosphoenolpyruvate (PEP) carboxylase (PEPCase) from orange fruit juice sacs has been cloned and heterogously expressed in high yield. The purified recombinant enzyme displays properties typical of plant PEPCase, including activation by sugar phosphates and inhibition by malate and citrate. Malate inhibition is weak in the physiological pH range, and the enzyme is also poorly affected by Glu and Asp, known inhibitors of C3 plants PEPCases. However, it is strongly inhibited by citrate. Orange fruit PEPCase phosphorylation by mammalian protein kinase A decreased inhibition by malate. The enzyme presents an unusual high molecular mass in the absence of PEP, while in its presence it displays a more common tetrameric arrangement. The overall properties of the enzyme suggest that it is suited for organic acid synthesis and NADH reoxidation in the mature fruit. The present study provides the first analysis of a recombinant fruit PEPCase.
publishDate 2010
dc.date.none.fl_str_mv 2010-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/84777
Perotti, Valeria Elisa; Figueroa, Carlos Maria; Andreo, Carlos Santiago; Iglesias, Alberto Alvaro; Podesta, Florencio Esteban; Cloning, Expression, Purification And Physical And Kinetic Characterization Of The Phosphoenolpyruvate Carboxylase From Orange (Citrus sinensis Osbeck var. Valencia) Fruit Juice Sacs; Elsevier; Plant Science; 179; 5; 11-2010; 527-535
0168-9452
CONICET Digital
CONICET
url http://hdl.handle.net/11336/84777
identifier_str_mv Perotti, Valeria Elisa; Figueroa, Carlos Maria; Andreo, Carlos Santiago; Iglesias, Alberto Alvaro; Podesta, Florencio Esteban; Cloning, Expression, Purification And Physical And Kinetic Characterization Of The Phosphoenolpyruvate Carboxylase From Orange (Citrus sinensis Osbeck var. Valencia) Fruit Juice Sacs; Elsevier; Plant Science; 179; 5; 11-2010; 527-535
0168-9452
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.plantsci.2010.08.003
info:eu-repo/semantics/altIdentifier/url/https://pubag.nal.usda.gov/catalog/792389
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1844613887280807936
score 13.070432

Publicaciones similares