Lactobacilli isolation from dental plaque and saliva of a group of patients with caries and characterization of their surface properties

Autores
Ahumada, María del Carmen; López, María Elena; Colloca, María Eugenia; Nader, Maria Elena Fatima
Año de publicación
2001
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The oral cavity is a complex ecosystem colonized by micro-organisms which play different roles. The aims of this study were to identify Lactobacillus strains from the teeth and saliva of children lacking in dental care and to study the surface characteristics related to the adhesion capability of these micro-organisms. The population considered is from Tucumán, Argentina. It is characterized by a dmfs index over 5 and an absence of dental care. Lactobacilli were isolated and identified by microscopic observations, biochemical tests and modified API CH 50. Bacterial surface studies included: hydrophobicity, acid and basic characteristics, blood cell agglutination and salt aggregation. Thirty strains were isolated. The major group was facultative heterofermentative. The surface characteristic studies did not indicate that lactobacilli are hydrophobic, neither do they show high basic nor acid charges. Lactobacilli isolated from saliva auto-agglutinated and also agglutinated ABO red blood cells. Salt aggregation was not a characteristic property. In this preliminary work, lactobacilli from teeth and saliva from this specific population were not demonstrated to have relevant adhesion properties.
Fil: Ahumada, María del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: López, María Elena. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Colloca, María Eugenia. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Materia
Dental Caries
Hydrophobicity
Lactobacilli
Saliva
Surface Characteristics
Teeth
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/55518
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Lactobacilli isolation from dental plaque and saliva of a group of patients with caries and characterization of their surface propertiesAhumada, María del CarmenLópez, María ElenaColloca, María EugeniaNader, Maria Elena FatimaDental CariesHydrophobicityLactobacilliSalivaSurface CharacteristicsTeethhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3The oral cavity is a complex ecosystem colonized by micro-organisms which play different roles. The aims of this study were to identify Lactobacillus strains from the teeth and saliva of children lacking in dental care and to study the surface characteristics related to the adhesion capability of these micro-organisms. The population considered is from Tucumán, Argentina. It is characterized by a dmfs index over 5 and an absence of dental care. Lactobacilli were isolated and identified by microscopic observations, biochemical tests and modified API CH 50. Bacterial surface studies included: hydrophobicity, acid and basic characteristics, blood cell agglutination and salt aggregation. Thirty strains were isolated. The major group was facultative heterofermentative. The surface characteristic studies did not indicate that lactobacilli are hydrophobic, neither do they show high basic nor acid charges. Lactobacilli isolated from saliva auto-agglutinated and also agglutinated ABO red blood cells. Salt aggregation was not a characteristic property. In this preliminary work, lactobacilli from teeth and saliva from this specific population were not demonstrated to have relevant adhesion properties.Fil: Ahumada, María del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: López, María Elena. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Colloca, María Eugenia. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; ArgentinaFil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaElsevier2001-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/55518Ahumada, María del Carmen; López, María Elena; Colloca, María Eugenia; Nader, Maria Elena Fatima; Lactobacilli isolation from dental plaque and saliva of a group of patients with caries and characterization of their surface properties; Elsevier; Anaerobe; 7; 2; 2-2001; 71-771075-9964CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1006/anae.2001.0372info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1075996401903725info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:11:11Zoai:ri.conicet.gov.ar:11336/55518instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:11:11.338CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Lactobacilli isolation from dental plaque and saliva of a group of patients with caries and characterization of their surface properties
title Lactobacilli isolation from dental plaque and saliva of a group of patients with caries and characterization of their surface properties
spellingShingle Lactobacilli isolation from dental plaque and saliva of a group of patients with caries and characterization of their surface properties
Ahumada, María del Carmen
Dental Caries
Hydrophobicity
Lactobacilli
Saliva
Surface Characteristics
Teeth
title_short Lactobacilli isolation from dental plaque and saliva of a group of patients with caries and characterization of their surface properties
title_full Lactobacilli isolation from dental plaque and saliva of a group of patients with caries and characterization of their surface properties
title_fullStr Lactobacilli isolation from dental plaque and saliva of a group of patients with caries and characterization of their surface properties
title_full_unstemmed Lactobacilli isolation from dental plaque and saliva of a group of patients with caries and characterization of their surface properties
title_sort Lactobacilli isolation from dental plaque and saliva of a group of patients with caries and characterization of their surface properties
dc.creator.none.fl_str_mv Ahumada, María del Carmen
López, María Elena
Colloca, María Eugenia
Nader, Maria Elena Fatima
author Ahumada, María del Carmen
author_facet Ahumada, María del Carmen
López, María Elena
Colloca, María Eugenia
Nader, Maria Elena Fatima
author_role author
author2 López, María Elena
Colloca, María Eugenia
Nader, Maria Elena Fatima
author2_role author
author
author
dc.subject.none.fl_str_mv Dental Caries
Hydrophobicity
Lactobacilli
Saliva
Surface Characteristics
Teeth
topic Dental Caries
Hydrophobicity
Lactobacilli
Saliva
Surface Characteristics
Teeth
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The oral cavity is a complex ecosystem colonized by micro-organisms which play different roles. The aims of this study were to identify Lactobacillus strains from the teeth and saliva of children lacking in dental care and to study the surface characteristics related to the adhesion capability of these micro-organisms. The population considered is from Tucumán, Argentina. It is characterized by a dmfs index over 5 and an absence of dental care. Lactobacilli were isolated and identified by microscopic observations, biochemical tests and modified API CH 50. Bacterial surface studies included: hydrophobicity, acid and basic characteristics, blood cell agglutination and salt aggregation. Thirty strains were isolated. The major group was facultative heterofermentative. The surface characteristic studies did not indicate that lactobacilli are hydrophobic, neither do they show high basic nor acid charges. Lactobacilli isolated from saliva auto-agglutinated and also agglutinated ABO red blood cells. Salt aggregation was not a characteristic property. In this preliminary work, lactobacilli from teeth and saliva from this specific population were not demonstrated to have relevant adhesion properties.
Fil: Ahumada, María del Carmen. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: López, María Elena. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Colloca, María Eugenia. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
description The oral cavity is a complex ecosystem colonized by micro-organisms which play different roles. The aims of this study were to identify Lactobacillus strains from the teeth and saliva of children lacking in dental care and to study the surface characteristics related to the adhesion capability of these micro-organisms. The population considered is from Tucumán, Argentina. It is characterized by a dmfs index over 5 and an absence of dental care. Lactobacilli were isolated and identified by microscopic observations, biochemical tests and modified API CH 50. Bacterial surface studies included: hydrophobicity, acid and basic characteristics, blood cell agglutination and salt aggregation. Thirty strains were isolated. The major group was facultative heterofermentative. The surface characteristic studies did not indicate that lactobacilli are hydrophobic, neither do they show high basic nor acid charges. Lactobacilli isolated from saliva auto-agglutinated and also agglutinated ABO red blood cells. Salt aggregation was not a characteristic property. In this preliminary work, lactobacilli from teeth and saliva from this specific population were not demonstrated to have relevant adhesion properties.
publishDate 2001
dc.date.none.fl_str_mv 2001-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/55518
Ahumada, María del Carmen; López, María Elena; Colloca, María Eugenia; Nader, Maria Elena Fatima; Lactobacilli isolation from dental plaque and saliva of a group of patients with caries and characterization of their surface properties; Elsevier; Anaerobe; 7; 2; 2-2001; 71-77
1075-9964
CONICET Digital
CONICET
url http://hdl.handle.net/11336/55518
identifier_str_mv Ahumada, María del Carmen; López, María Elena; Colloca, María Eugenia; Nader, Maria Elena Fatima; Lactobacilli isolation from dental plaque and saliva of a group of patients with caries and characterization of their surface properties; Elsevier; Anaerobe; 7; 2; 2-2001; 71-77
1075-9964
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1006/anae.2001.0372
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1075996401903725
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/msword
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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