Surface properties of lactobacilli isolated from healthy subjects

Autores
Colloca, M. E.; Ahumada, M. C.; Lopez de Bocanera, Maria Elena; Nader, Maria Elena Fatima
Año de publicación
2008
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
OBJECTIVE: Lactobacilli are considered cariogenic micro-organismS. As oral species of lactobacilli have not been thoroughly described, the aim of this work was to isolate and identify these organisms from teeth, tongue, saliva and gum of healthy patients and to describe some of their surface properties. SUBJECTS: Forty-four subjects from Tucumán, Argentina, with D, d and M, m indices equal to 0. MATERIALS AND METHODS: Samples were obtained from different areas of the oral cavity. Microorganisms were cultured in lactobacilli selected media (LBS) and identified morphologically and biochemically. Hydrophobicity was analysed by partition in organic solvents, acidity by affinity with chloroform and basicity with ethyl acetate (MATH method), aggregation and coaggregation in presence of (NH4)2SO4, and haemagglutination with ABO erythrocytes in microplates. RESULTS: Eighty-five lactobacilli were isolated; 29.4% were homofermenter, 44.7% facultative heterofermenter and 25.9% obligate heterofermenter. Predominant species were L.fermentum, L.plantarum, L.salivarius, and L.rhamnosus. Most of the strains showed moderate to high hydrophobicity and demonstrated high acid and basic surface charges with almost 40% showing salt aggregation. Few strains haemagglutinated. CONCLUSIONS: A variety of Lactobacillus species were isolated from healthy mouths, some of whom showed adhesion-related properties such as high hydrophobicity and charged surfaceS. Probable mechanisms related to the ecological behaviour of lactobacilli in the oral cavity are discussed.
Fil: Colloca, M. E.. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Ahumada, M. C.. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Lopez de Bocanera, Maria Elena. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Materia
Lactobacilli
Oral Health
Surface Characteristics
Adhesion
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/38275
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/38275
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Surface properties of lactobacilli isolated from healthy subjectsColloca, M. E.Ahumada, M. C.Lopez de Bocanera, Maria ElenaNader, Maria Elena FatimaLactobacilliOral HealthSurface CharacteristicsAdhesionhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3OBJECTIVE: Lactobacilli are considered cariogenic micro-organismS. As oral species of lactobacilli have not been thoroughly described, the aim of this work was to isolate and identify these organisms from teeth, tongue, saliva and gum of healthy patients and to describe some of their surface properties. SUBJECTS: Forty-four subjects from Tucumán, Argentina, with D, d and M, m indices equal to 0. MATERIALS AND METHODS: Samples were obtained from different areas of the oral cavity. Microorganisms were cultured in lactobacilli selected media (LBS) and identified morphologically and biochemically. Hydrophobicity was analysed by partition in organic solvents, acidity by affinity with chloroform and basicity with ethyl acetate (MATH method), aggregation and coaggregation in presence of (NH4)2SO4, and haemagglutination with ABO erythrocytes in microplates. RESULTS: Eighty-five lactobacilli were isolated; 29.4% were homofermenter, 44.7% facultative heterofermenter and 25.9% obligate heterofermenter. Predominant species were L.fermentum, L.plantarum, L.salivarius, and L.rhamnosus. Most of the strains showed moderate to high hydrophobicity and demonstrated high acid and basic surface charges with almost 40% showing salt aggregation. Few strains haemagglutinated. CONCLUSIONS: A variety of Lactobacillus species were isolated from healthy mouths, some of whom showed adhesion-related properties such as high hydrophobicity and charged surfaceS. Probable mechanisms related to the ecological behaviour of lactobacilli in the oral cavity are discussed.Fil: Colloca, M. E.. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Ahumada, M. C.. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Lopez de Bocanera, Maria Elena. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaFil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; ArgentinaWiley Blackwell Publishing, Inc2008-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/mswordapplication/pdfhttp://hdl.handle.net/11336/38275Colloca, M. E.; Ahumada, M. C.; Lopez de Bocanera, Maria Elena; Nader, Maria Elena Fatima; Surface properties of lactobacilli isolated from healthy subjects; Wiley Blackwell Publishing, Inc; Oral Diseases; 6; 4; 6-2008; 227-2331354-523XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1601-0825.2000.tb00118.xinfo:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1601-0825.2000.tb00118.x/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:07:02Zoai:ri.conicet.gov.ar:11336/38275instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:07:02.384CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Surface properties of lactobacilli isolated from healthy subjects
title Surface properties of lactobacilli isolated from healthy subjects
spellingShingle Surface properties of lactobacilli isolated from healthy subjects
Colloca, M. E.
Lactobacilli
Oral Health
Surface Characteristics
Adhesion
title_short Surface properties of lactobacilli isolated from healthy subjects
title_full Surface properties of lactobacilli isolated from healthy subjects
title_fullStr Surface properties of lactobacilli isolated from healthy subjects
title_full_unstemmed Surface properties of lactobacilli isolated from healthy subjects
title_sort Surface properties of lactobacilli isolated from healthy subjects
dc.creator.none.fl_str_mv Colloca, M. E.
Ahumada, M. C.
Lopez de Bocanera, Maria Elena
Nader, Maria Elena Fatima
author Colloca, M. E.
author_facet Colloca, M. E.
Ahumada, M. C.
Lopez de Bocanera, Maria Elena
Nader, Maria Elena Fatima
author_role author
author2 Ahumada, M. C.
Lopez de Bocanera, Maria Elena
Nader, Maria Elena Fatima
author2_role author
author
author
dc.subject.none.fl_str_mv Lactobacilli
Oral Health
Surface Characteristics
Adhesion
topic Lactobacilli
Oral Health
Surface Characteristics
Adhesion
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv OBJECTIVE: Lactobacilli are considered cariogenic micro-organismS. As oral species of lactobacilli have not been thoroughly described, the aim of this work was to isolate and identify these organisms from teeth, tongue, saliva and gum of healthy patients and to describe some of their surface properties. SUBJECTS: Forty-four subjects from Tucumán, Argentina, with D, d and M, m indices equal to 0. MATERIALS AND METHODS: Samples were obtained from different areas of the oral cavity. Microorganisms were cultured in lactobacilli selected media (LBS) and identified morphologically and biochemically. Hydrophobicity was analysed by partition in organic solvents, acidity by affinity with chloroform and basicity with ethyl acetate (MATH method), aggregation and coaggregation in presence of (NH4)2SO4, and haemagglutination with ABO erythrocytes in microplates. RESULTS: Eighty-five lactobacilli were isolated; 29.4% were homofermenter, 44.7% facultative heterofermenter and 25.9% obligate heterofermenter. Predominant species were L.fermentum, L.plantarum, L.salivarius, and L.rhamnosus. Most of the strains showed moderate to high hydrophobicity and demonstrated high acid and basic surface charges with almost 40% showing salt aggregation. Few strains haemagglutinated. CONCLUSIONS: A variety of Lactobacillus species were isolated from healthy mouths, some of whom showed adhesion-related properties such as high hydrophobicity and charged surfaceS. Probable mechanisms related to the ecological behaviour of lactobacilli in the oral cavity are discussed.
Fil: Colloca, M. E.. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Ahumada, M. C.. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Lopez de Bocanera, Maria Elena. Universidad Nacional de Tucumán. Facultad de Odontología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
Fil: Nader, Maria Elena Fatima. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Centro de Referencia Para Lactobacilos; Argentina
description OBJECTIVE: Lactobacilli are considered cariogenic micro-organismS. As oral species of lactobacilli have not been thoroughly described, the aim of this work was to isolate and identify these organisms from teeth, tongue, saliva and gum of healthy patients and to describe some of their surface properties. SUBJECTS: Forty-four subjects from Tucumán, Argentina, with D, d and M, m indices equal to 0. MATERIALS AND METHODS: Samples were obtained from different areas of the oral cavity. Microorganisms were cultured in lactobacilli selected media (LBS) and identified morphologically and biochemically. Hydrophobicity was analysed by partition in organic solvents, acidity by affinity with chloroform and basicity with ethyl acetate (MATH method), aggregation and coaggregation in presence of (NH4)2SO4, and haemagglutination with ABO erythrocytes in microplates. RESULTS: Eighty-five lactobacilli were isolated; 29.4% were homofermenter, 44.7% facultative heterofermenter and 25.9% obligate heterofermenter. Predominant species were L.fermentum, L.plantarum, L.salivarius, and L.rhamnosus. Most of the strains showed moderate to high hydrophobicity and demonstrated high acid and basic surface charges with almost 40% showing salt aggregation. Few strains haemagglutinated. CONCLUSIONS: A variety of Lactobacillus species were isolated from healthy mouths, some of whom showed adhesion-related properties such as high hydrophobicity and charged surfaceS. Probable mechanisms related to the ecological behaviour of lactobacilli in the oral cavity are discussed.
publishDate 2008
dc.date.none.fl_str_mv 2008-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/38275
Colloca, M. E.; Ahumada, M. C.; Lopez de Bocanera, Maria Elena; Nader, Maria Elena Fatima; Surface properties of lactobacilli isolated from healthy subjects; Wiley Blackwell Publishing, Inc; Oral Diseases; 6; 4; 6-2008; 227-233
1354-523X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/38275
identifier_str_mv Colloca, M. E.; Ahumada, M. C.; Lopez de Bocanera, Maria Elena; Nader, Maria Elena Fatima; Surface properties of lactobacilli isolated from healthy subjects; Wiley Blackwell Publishing, Inc; Oral Diseases; 6; 4; 6-2008; 227-233
1354-523X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1601-0825.2000.tb00118.x
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1601-0825.2000.tb00118.x/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/msword
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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