Down Modulation of TNF-α mRNA Placental Expression by AZT Used for the Prevention of HIV-1 Mother-to-Child Transmission

Autores
Pornprasert, Sakorn; Faye, Albert; Mary, Jean Yves; Dolcini, Guillermina Laura; Leechanachai, Pranee; Chaouat, Gérard; Ngo, Nicole; Barré Sinoussi, Françoise; Menu, Elizabeth
Año de publicación
2006
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mechanisms of HIV-1 in utero mother-to-child transmission (MTCT) protection provided by AZT are not completely understood. The placental cytokine network is involved in the control of HIV-1 in utero transmission but the effect of AZT on this network is unknown. To evaluate the effects of AZT on placental cytokine expression, the chorionic villi from HIV-1 uninfected women term placentae were cultured with 0, 100, and 2000ng/ml AZT. Tissue fragments were harvested at days 1, 4, and 7 to determine the level of cytokine mRNA by real-time RT-PCR. The viability and morphology of the placental histocultures were monitored by the expression of beta-human chorionic gonadotropin (beta-hCG) gene, lipopolysaccharide (LPS) activation, and microscopic examination. AZT at 2000ng/ml significantly down-regulated TNF-alpha mRNA expression at day 1 and day 4, but had no effect on beta-hCG, stromal cell-derived factor 1 (SDF-1), and IL-10 gene expression. AZT did not induce any deleterious impact on placental tissue structure. Furthermore, activation of chorionic villi by LPS for 24h up-regulated IL-10 and TNF-alpha mRNA expression. Down-regulation of TNF-alpha mRNA could represent a mechanism through which AZT can decrease the risk of HIV-1 MTCT, in addition to its direct effect on HIV-1 replication.
Fil: Pornprasert, Sakorn. Institut Pasteur de Paris; Francia. Mahidol University; Tailandia. Chiang Mai University; Tailandia
Fil: Faye, Albert. Institut National de la Santé et de la Recherche Médicale; Francia. Institut Pasteur de Paris; Francia
Fil: Mary, Jean Yves. Institut National de la Santé et de la Recherche Médicale; Francia. Université Paris Diderot - Paris 7; Francia
Fil: Dolcini, Guillermina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Institut Pasteur de Paris; Francia
Fil: Leechanachai, Pranee. Chiang Mai University; Tailandia
Fil: Chaouat, Gérard. Institut National de la Santé et de la Recherche Médicale; Francia
Fil: Ngo, Nicole. Chiang Mai University; Tailandia
Fil: Barré Sinoussi, Françoise. Institut Pasteur de Paris; Francia
Fil: Menu, Elizabeth. Institut National de la Santé et de la Recherche Médicale; Francia. Institut Pasteur de Paris; Francia
Materia
AZT
HIV-1
HISTOCULTURE
PLACENTAL CYTOKINE EXPRESSION
MOTHER-TO-CHILD TRANSMISSION
PREVENTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/105985
Acceder
id CONICETDig_b2d3542324327fe0bd75d749bf45c956
oai_identifier_str oai:ri.conicet.gov.ar:11336/105985
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Down Modulation of TNF-α mRNA Placental Expression by AZT Used for the Prevention of HIV-1 Mother-to-Child TransmissionPornprasert, SakornFaye, AlbertMary, Jean YvesDolcini, Guillermina LauraLeechanachai, PraneeChaouat, GérardNgo, NicoleBarré Sinoussi, FrançoiseMenu, ElizabethAZTHIV-1HISTOCULTUREPLACENTAL CYTOKINE EXPRESSIONMOTHER-TO-CHILD TRANSMISSIONPREVENTIONhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Mechanisms of HIV-1 in utero mother-to-child transmission (MTCT) protection provided by AZT are not completely understood. The placental cytokine network is involved in the control of HIV-1 in utero transmission but the effect of AZT on this network is unknown. To evaluate the effects of AZT on placental cytokine expression, the chorionic villi from HIV-1 uninfected women term placentae were cultured with 0, 100, and 2000ng/ml AZT. Tissue fragments were harvested at days 1, 4, and 7 to determine the level of cytokine mRNA by real-time RT-PCR. The viability and morphology of the placental histocultures were monitored by the expression of beta-human chorionic gonadotropin (beta-hCG) gene, lipopolysaccharide (LPS) activation, and microscopic examination. AZT at 2000ng/ml significantly down-regulated TNF-alpha mRNA expression at day 1 and day 4, but had no effect on beta-hCG, stromal cell-derived factor 1 (SDF-1), and IL-10 gene expression. AZT did not induce any deleterious impact on placental tissue structure. Furthermore, activation of chorionic villi by LPS for 24h up-regulated IL-10 and TNF-alpha mRNA expression. Down-regulation of TNF-alpha mRNA could represent a mechanism through which AZT can decrease the risk of HIV-1 MTCT, in addition to its direct effect on HIV-1 replication.Fil: Pornprasert, Sakorn. Institut Pasteur de Paris; Francia. Mahidol University; Tailandia. Chiang Mai University; TailandiaFil: Faye, Albert. Institut National de la Santé et de la Recherche Médicale; Francia. Institut Pasteur de Paris; FranciaFil: Mary, Jean Yves. Institut National de la Santé et de la Recherche Médicale; Francia. Université Paris Diderot - Paris 7; FranciaFil: Dolcini, Guillermina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Institut Pasteur de Paris; FranciaFil: Leechanachai, Pranee. Chiang Mai University; TailandiaFil: Chaouat, Gérard. Institut National de la Santé et de la Recherche Médicale; FranciaFil: Ngo, Nicole. Chiang Mai University; TailandiaFil: Barré Sinoussi, Françoise. Institut Pasteur de Paris; FranciaFil: Menu, Elizabeth. Institut National de la Santé et de la Recherche Médicale; Francia. Institut Pasteur de Paris; FranciaW B Saunders Co Ltd2006-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/105985Pornprasert, Sakorn; Faye, Albert; Mary, Jean Yves; Dolcini, Guillermina Laura; Leechanachai, Pranee; et al.; Down Modulation of TNF-α mRNA Placental Expression by AZT Used for the Prevention of HIV-1 Mother-to-Child Transmission; W B Saunders Co Ltd; Placenta; 27; 9-10; 9-2006; 989-9950143-40041532-3102CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.placenta.2005.10.010info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0143400405002833info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-12T09:53:16Zoai:ri.conicet.gov.ar:11336/105985instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-12 09:53:16.444CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Down Modulation of TNF-α mRNA Placental Expression by AZT Used for the Prevention of HIV-1 Mother-to-Child Transmission
title Down Modulation of TNF-α mRNA Placental Expression by AZT Used for the Prevention of HIV-1 Mother-to-Child Transmission
spellingShingle Down Modulation of TNF-α mRNA Placental Expression by AZT Used for the Prevention of HIV-1 Mother-to-Child Transmission
Pornprasert, Sakorn
AZT
HIV-1
HISTOCULTURE
PLACENTAL CYTOKINE EXPRESSION
MOTHER-TO-CHILD TRANSMISSION
PREVENTION
title_short Down Modulation of TNF-α mRNA Placental Expression by AZT Used for the Prevention of HIV-1 Mother-to-Child Transmission
title_full Down Modulation of TNF-α mRNA Placental Expression by AZT Used for the Prevention of HIV-1 Mother-to-Child Transmission
title_fullStr Down Modulation of TNF-α mRNA Placental Expression by AZT Used for the Prevention of HIV-1 Mother-to-Child Transmission
title_full_unstemmed Down Modulation of TNF-α mRNA Placental Expression by AZT Used for the Prevention of HIV-1 Mother-to-Child Transmission
title_sort Down Modulation of TNF-α mRNA Placental Expression by AZT Used for the Prevention of HIV-1 Mother-to-Child Transmission
dc.creator.none.fl_str_mv Pornprasert, Sakorn
Faye, Albert
Mary, Jean Yves
Dolcini, Guillermina Laura
Leechanachai, Pranee
Chaouat, Gérard
Ngo, Nicole
Barré Sinoussi, Françoise
Menu, Elizabeth
author Pornprasert, Sakorn
author_facet Pornprasert, Sakorn
Faye, Albert
Mary, Jean Yves
Dolcini, Guillermina Laura
Leechanachai, Pranee
Chaouat, Gérard
Ngo, Nicole
Barré Sinoussi, Françoise
Menu, Elizabeth
author_role author
author2 Faye, Albert
Mary, Jean Yves
Dolcini, Guillermina Laura
Leechanachai, Pranee
Chaouat, Gérard
Ngo, Nicole
Barré Sinoussi, Françoise
Menu, Elizabeth
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv AZT
HIV-1
HISTOCULTURE
PLACENTAL CYTOKINE EXPRESSION
MOTHER-TO-CHILD TRANSMISSION
PREVENTION
topic AZT
HIV-1
HISTOCULTURE
PLACENTAL CYTOKINE EXPRESSION
MOTHER-TO-CHILD TRANSMISSION
PREVENTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Mechanisms of HIV-1 in utero mother-to-child transmission (MTCT) protection provided by AZT are not completely understood. The placental cytokine network is involved in the control of HIV-1 in utero transmission but the effect of AZT on this network is unknown. To evaluate the effects of AZT on placental cytokine expression, the chorionic villi from HIV-1 uninfected women term placentae were cultured with 0, 100, and 2000ng/ml AZT. Tissue fragments were harvested at days 1, 4, and 7 to determine the level of cytokine mRNA by real-time RT-PCR. The viability and morphology of the placental histocultures were monitored by the expression of beta-human chorionic gonadotropin (beta-hCG) gene, lipopolysaccharide (LPS) activation, and microscopic examination. AZT at 2000ng/ml significantly down-regulated TNF-alpha mRNA expression at day 1 and day 4, but had no effect on beta-hCG, stromal cell-derived factor 1 (SDF-1), and IL-10 gene expression. AZT did not induce any deleterious impact on placental tissue structure. Furthermore, activation of chorionic villi by LPS for 24h up-regulated IL-10 and TNF-alpha mRNA expression. Down-regulation of TNF-alpha mRNA could represent a mechanism through which AZT can decrease the risk of HIV-1 MTCT, in addition to its direct effect on HIV-1 replication.
Fil: Pornprasert, Sakorn. Institut Pasteur de Paris; Francia. Mahidol University; Tailandia. Chiang Mai University; Tailandia
Fil: Faye, Albert. Institut National de la Santé et de la Recherche Médicale; Francia. Institut Pasteur de Paris; Francia
Fil: Mary, Jean Yves. Institut National de la Santé et de la Recherche Médicale; Francia. Université Paris Diderot - Paris 7; Francia
Fil: Dolcini, Guillermina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Institut Pasteur de Paris; Francia
Fil: Leechanachai, Pranee. Chiang Mai University; Tailandia
Fil: Chaouat, Gérard. Institut National de la Santé et de la Recherche Médicale; Francia
Fil: Ngo, Nicole. Chiang Mai University; Tailandia
Fil: Barré Sinoussi, Françoise. Institut Pasteur de Paris; Francia
Fil: Menu, Elizabeth. Institut National de la Santé et de la Recherche Médicale; Francia. Institut Pasteur de Paris; Francia
description Mechanisms of HIV-1 in utero mother-to-child transmission (MTCT) protection provided by AZT are not completely understood. The placental cytokine network is involved in the control of HIV-1 in utero transmission but the effect of AZT on this network is unknown. To evaluate the effects of AZT on placental cytokine expression, the chorionic villi from HIV-1 uninfected women term placentae were cultured with 0, 100, and 2000ng/ml AZT. Tissue fragments were harvested at days 1, 4, and 7 to determine the level of cytokine mRNA by real-time RT-PCR. The viability and morphology of the placental histocultures were monitored by the expression of beta-human chorionic gonadotropin (beta-hCG) gene, lipopolysaccharide (LPS) activation, and microscopic examination. AZT at 2000ng/ml significantly down-regulated TNF-alpha mRNA expression at day 1 and day 4, but had no effect on beta-hCG, stromal cell-derived factor 1 (SDF-1), and IL-10 gene expression. AZT did not induce any deleterious impact on placental tissue structure. Furthermore, activation of chorionic villi by LPS for 24h up-regulated IL-10 and TNF-alpha mRNA expression. Down-regulation of TNF-alpha mRNA could represent a mechanism through which AZT can decrease the risk of HIV-1 MTCT, in addition to its direct effect on HIV-1 replication.
publishDate 2006
dc.date.none.fl_str_mv 2006-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/105985
Pornprasert, Sakorn; Faye, Albert; Mary, Jean Yves; Dolcini, Guillermina Laura; Leechanachai, Pranee; et al.; Down Modulation of TNF-α mRNA Placental Expression by AZT Used for the Prevention of HIV-1 Mother-to-Child Transmission; W B Saunders Co Ltd; Placenta; 27; 9-10; 9-2006; 989-995
0143-4004
1532-3102
CONICET Digital
CONICET
url http://hdl.handle.net/11336/105985
identifier_str_mv Pornprasert, Sakorn; Faye, Albert; Mary, Jean Yves; Dolcini, Guillermina Laura; Leechanachai, Pranee; et al.; Down Modulation of TNF-α mRNA Placental Expression by AZT Used for the Prevention of HIV-1 Mother-to-Child Transmission; W B Saunders Co Ltd; Placenta; 27; 9-10; 9-2006; 989-995
0143-4004
1532-3102
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.placenta.2005.10.010
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0143400405002833
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv W B Saunders Co Ltd
publisher.none.fl_str_mv W B Saunders Co Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.003704

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