Evaluation of the placental environment with a new in vitro model of histocultures of early and term placentae: determination of cytokine and chemokine expression profiles

Autores
Faye, Albert; Pornprasert, Sakorn; Dolcini, Guillermina Laura; Avé, Patrick; Taïeb, Joélle Z.; Taupin, Jean Luc; Derrien, Muriel; Huerre, Michel René; Barré Sinoussi, Françoise; Chaouat, Gerard; Menu, Elizabeth
Año de publicación
2005
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We aimed to set up and validate a new in vitro model of placental histocultures, for the evaluation of cytokine and chemokine profiles of the placental environment, over a long culture period. Micro-explant cultures from 6 early and 6 term placentae were set up on collagen sponge gel supports at a liquid/air interface. At various times during culture, we analyzed tissue morphology and cell death by microscopy and quantified beta-hCG production and mRNA levels for beta-hCG and insulin-like 4 (INSL4). Levels of IL-6, LIF, TNF alpha, IL-10, IFN-gamma, IL-16 and RANTES in the medium were measured by ELISA on days 1, 4 and 7 of culture. SDF-1 mRNA expression was determined by real-time PCR at the same time points. Histocultures from early and term placentae remained viable until day 10. High levels of IL-6 and LIF production, low levels of TNF alpha, IL-10 and IFN-gamma production and significant SDF-1 expression were observed. These data indicate that placental histoculture is a suitable and reliable in vitro model for studying the placental environment.
Fil: Faye, Albert. Institut Pasteur de Paris; Francia. Institut National de la Santé et de la Recherche Médicale; Francia
Fil: Pornprasert, Sakorn. Institut Pasteur de Paris; Francia
Fil: Dolcini, Guillermina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Institut Pasteur de Paris; Francia
Fil: Avé, Patrick. Institut Pasteur de Paris; Francia
Fil: Taïeb, Joélle Z.. Antoine Béclère Hospital. Biochemistry Unit; Francia
Fil: Taupin, Jean Luc. Centre National de la Recherche Scientifique; Francia. Universite de Bordeaux; Francia
Fil: Derrien, Muriel. Institut Pasteur de Paris; Francia
Fil: Huerre, Michel René. Institut Pasteur de Paris; Francia
Fil: Barré Sinoussi, Françoise. Institut Pasteur de Paris; Francia
Fil: Chaouat, Gerard. Institut National de la Santé et de la Recherche Médicale; Francia
Fil: Menu, Elizabeth. Institut Pasteur de Paris; Francia. Institut National de la Santé et de la Recherche Médicale; Francia
Materia
CYTOKINE
CHEMOKINE
PLACENTA
ENVIRONMENT HISTOCULTURE
HISTOCULTURE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/105983
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/105983
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Evaluation of the placental environment with a new in vitro model of histocultures of early and term placentae: determination of cytokine and chemokine expression profilesFaye, AlbertPornprasert, SakornDolcini, Guillermina LauraAvé, PatrickTaïeb, Joélle Z.Taupin, Jean LucDerrien, MurielHuerre, Michel RenéBarré Sinoussi, FrançoiseChaouat, GerardMenu, ElizabethCYTOKINECHEMOKINEPLACENTAENVIRONMENT HISTOCULTUREHISTOCULTUREhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1We aimed to set up and validate a new in vitro model of placental histocultures, for the evaluation of cytokine and chemokine profiles of the placental environment, over a long culture period. Micro-explant cultures from 6 early and 6 term placentae were set up on collagen sponge gel supports at a liquid/air interface. At various times during culture, we analyzed tissue morphology and cell death by microscopy and quantified beta-hCG production and mRNA levels for beta-hCG and insulin-like 4 (INSL4). Levels of IL-6, LIF, TNF alpha, IL-10, IFN-gamma, IL-16 and RANTES in the medium were measured by ELISA on days 1, 4 and 7 of culture. SDF-1 mRNA expression was determined by real-time PCR at the same time points. Histocultures from early and term placentae remained viable until day 10. High levels of IL-6 and LIF production, low levels of TNF alpha, IL-10 and IFN-gamma production and significant SDF-1 expression were observed. These data indicate that placental histoculture is a suitable and reliable in vitro model for studying the placental environment.Fil: Faye, Albert. Institut Pasteur de Paris; Francia. Institut National de la Santé et de la Recherche Médicale; FranciaFil: Pornprasert, Sakorn. Institut Pasteur de Paris; FranciaFil: Dolcini, Guillermina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Institut Pasteur de Paris; FranciaFil: Avé, Patrick. Institut Pasteur de Paris; FranciaFil: Taïeb, Joélle Z.. Antoine Béclère Hospital. Biochemistry Unit; FranciaFil: Taupin, Jean Luc. Centre National de la Recherche Scientifique; Francia. Universite de Bordeaux; FranciaFil: Derrien, Muriel. Institut Pasteur de Paris; FranciaFil: Huerre, Michel René. Institut Pasteur de Paris; FranciaFil: Barré Sinoussi, Françoise. Institut Pasteur de Paris; FranciaFil: Chaouat, Gerard. Institut National de la Santé et de la Recherche Médicale; FranciaFil: Menu, Elizabeth. Institut Pasteur de Paris; Francia. Institut National de la Santé et de la Recherche Médicale; FranciaW B Saunders Co Ltd2005-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/105983Faye, Albert; Pornprasert, Sakorn; Dolcini, Guillermina Laura; Avé, Patrick; Taïeb, Joélle Z.; et al.; Evaluation of the placental environment with a new in vitro model of histocultures of early and term placentae: determination of cytokine and chemokine expression profiles; W B Saunders Co Ltd; Placenta; 26; 2-3; 2-2005; 262-2670143-40041532-3102CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.placenta.2004.08.005info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:30:13Zoai:ri.conicet.gov.ar:11336/105983instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:30:13.474CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Evaluation of the placental environment with a new in vitro model of histocultures of early and term placentae: determination of cytokine and chemokine expression profiles
title Evaluation of the placental environment with a new in vitro model of histocultures of early and term placentae: determination of cytokine and chemokine expression profiles
spellingShingle Evaluation of the placental environment with a new in vitro model of histocultures of early and term placentae: determination of cytokine and chemokine expression profiles
Faye, Albert
CYTOKINE
CHEMOKINE
PLACENTA
ENVIRONMENT HISTOCULTURE
HISTOCULTURE
title_short Evaluation of the placental environment with a new in vitro model of histocultures of early and term placentae: determination of cytokine and chemokine expression profiles
title_full Evaluation of the placental environment with a new in vitro model of histocultures of early and term placentae: determination of cytokine and chemokine expression profiles
title_fullStr Evaluation of the placental environment with a new in vitro model of histocultures of early and term placentae: determination of cytokine and chemokine expression profiles
title_full_unstemmed Evaluation of the placental environment with a new in vitro model of histocultures of early and term placentae: determination of cytokine and chemokine expression profiles
title_sort Evaluation of the placental environment with a new in vitro model of histocultures of early and term placentae: determination of cytokine and chemokine expression profiles
dc.creator.none.fl_str_mv Faye, Albert
Pornprasert, Sakorn
Dolcini, Guillermina Laura
Avé, Patrick
Taïeb, Joélle Z.
Taupin, Jean Luc
Derrien, Muriel
Huerre, Michel René
Barré Sinoussi, Françoise
Chaouat, Gerard
Menu, Elizabeth
author Faye, Albert
author_facet Faye, Albert
Pornprasert, Sakorn
Dolcini, Guillermina Laura
Avé, Patrick
Taïeb, Joélle Z.
Taupin, Jean Luc
Derrien, Muriel
Huerre, Michel René
Barré Sinoussi, Françoise
Chaouat, Gerard
Menu, Elizabeth
author_role author
author2 Pornprasert, Sakorn
Dolcini, Guillermina Laura
Avé, Patrick
Taïeb, Joélle Z.
Taupin, Jean Luc
Derrien, Muriel
Huerre, Michel René
Barré Sinoussi, Françoise
Chaouat, Gerard
Menu, Elizabeth
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv CYTOKINE
CHEMOKINE
PLACENTA
ENVIRONMENT HISTOCULTURE
HISTOCULTURE
topic CYTOKINE
CHEMOKINE
PLACENTA
ENVIRONMENT HISTOCULTURE
HISTOCULTURE
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv We aimed to set up and validate a new in vitro model of placental histocultures, for the evaluation of cytokine and chemokine profiles of the placental environment, over a long culture period. Micro-explant cultures from 6 early and 6 term placentae were set up on collagen sponge gel supports at a liquid/air interface. At various times during culture, we analyzed tissue morphology and cell death by microscopy and quantified beta-hCG production and mRNA levels for beta-hCG and insulin-like 4 (INSL4). Levels of IL-6, LIF, TNF alpha, IL-10, IFN-gamma, IL-16 and RANTES in the medium were measured by ELISA on days 1, 4 and 7 of culture. SDF-1 mRNA expression was determined by real-time PCR at the same time points. Histocultures from early and term placentae remained viable until day 10. High levels of IL-6 and LIF production, low levels of TNF alpha, IL-10 and IFN-gamma production and significant SDF-1 expression were observed. These data indicate that placental histoculture is a suitable and reliable in vitro model for studying the placental environment.
Fil: Faye, Albert. Institut Pasteur de Paris; Francia. Institut National de la Santé et de la Recherche Médicale; Francia
Fil: Pornprasert, Sakorn. Institut Pasteur de Paris; Francia
Fil: Dolcini, Guillermina Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Institut Pasteur de Paris; Francia
Fil: Avé, Patrick. Institut Pasteur de Paris; Francia
Fil: Taïeb, Joélle Z.. Antoine Béclère Hospital. Biochemistry Unit; Francia
Fil: Taupin, Jean Luc. Centre National de la Recherche Scientifique; Francia. Universite de Bordeaux; Francia
Fil: Derrien, Muriel. Institut Pasteur de Paris; Francia
Fil: Huerre, Michel René. Institut Pasteur de Paris; Francia
Fil: Barré Sinoussi, Françoise. Institut Pasteur de Paris; Francia
Fil: Chaouat, Gerard. Institut National de la Santé et de la Recherche Médicale; Francia
Fil: Menu, Elizabeth. Institut Pasteur de Paris; Francia. Institut National de la Santé et de la Recherche Médicale; Francia
description We aimed to set up and validate a new in vitro model of placental histocultures, for the evaluation of cytokine and chemokine profiles of the placental environment, over a long culture period. Micro-explant cultures from 6 early and 6 term placentae were set up on collagen sponge gel supports at a liquid/air interface. At various times during culture, we analyzed tissue morphology and cell death by microscopy and quantified beta-hCG production and mRNA levels for beta-hCG and insulin-like 4 (INSL4). Levels of IL-6, LIF, TNF alpha, IL-10, IFN-gamma, IL-16 and RANTES in the medium were measured by ELISA on days 1, 4 and 7 of culture. SDF-1 mRNA expression was determined by real-time PCR at the same time points. Histocultures from early and term placentae remained viable until day 10. High levels of IL-6 and LIF production, low levels of TNF alpha, IL-10 and IFN-gamma production and significant SDF-1 expression were observed. These data indicate that placental histoculture is a suitable and reliable in vitro model for studying the placental environment.
publishDate 2005
dc.date.none.fl_str_mv 2005-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/105983
Faye, Albert; Pornprasert, Sakorn; Dolcini, Guillermina Laura; Avé, Patrick; Taïeb, Joélle Z.; et al.; Evaluation of the placental environment with a new in vitro model of histocultures of early and term placentae: determination of cytokine and chemokine expression profiles; W B Saunders Co Ltd; Placenta; 26; 2-3; 2-2005; 262-267
0143-4004
1532-3102
CONICET Digital
CONICET
url http://hdl.handle.net/11336/105983
identifier_str_mv Faye, Albert; Pornprasert, Sakorn; Dolcini, Guillermina Laura; Avé, Patrick; Taïeb, Joélle Z.; et al.; Evaluation of the placental environment with a new in vitro model of histocultures of early and term placentae: determination of cytokine and chemokine expression profiles; W B Saunders Co Ltd; Placenta; 26; 2-3; 2-2005; 262-267
0143-4004
1532-3102
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.placenta.2004.08.005
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv W B Saunders Co Ltd
publisher.none.fl_str_mv W B Saunders Co Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082777901957120
score 13.22299

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