Higher placental anti-inflammatory IL-10 cytokine expression in HIV-1 infected women receiving longer zidovudine prophylaxis associated with nevirapine

• Autores: Pornprasert, Sakorn; Mary, Jean-Yves; Faye, Albert; Leechanachai, Pannee; Limtrakul, Aram; Rugpao, Sungwal; Sirivatanapa, Pannee; Gomuthbutra, Vorapin; Matanasaravoot, Wanmanee; Le Coer, Sophie; Lallemant, Marc; Barré-Sinoussi, Françoise; Menu, Elisabeth; Ngo Giang Huong, Nicole; Ayouba, Ahidjo; Chailert, Sanupong; Chaouat, Gérard; Derrien, Muriel; Dolcini, Guillermina Laura; Eteki, Nicole; Kfutwah, Anfumbom Jude; Kouo, Odette; Lemen, Brigitte; Abal, Facundo Juan Pablo; Nerrienet, Eric; Njinku, Bernadette; Scarlatti, Gabriella; Tejiokem, Mathurin; Téné, Gilbert
• Año de publicación: 2009
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: ANTIRETROVIRAL PROPHYLAXIS; HIV-1; IL-10 CYTOKINE; MOTHER-TO-CHILD TRANSMISSION; PLACENTA; ZIDOVUDINE AND NEVIRAPINE
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

Placental cytokine balance may be critical for the control of mother-to-child transmission (MTCT) of HIV. We assessed whether the type and duration of antiretrovirals used for prevention of HIV-1-MTCT modified the inflammatory cytokine profile. We investigated the levels of cytokine expression in the placentas of 61 HIV-1-infected women who received zidovudine (ZDV) plus single dose nevirapine (SD-NVP) or ZDV only for prevention of MTCT. Placentas of 38 HIV-1-uninfected women were included as controls. All placentas were obtained after vaginal delivery. Levels of mRNA and cytokine expression were quantified using real-time PCR and ELISA, respectively, in placental explants and 24-hour culture supernatants and analyzed in relation to the women's characteristics and the type and duration of antiretroviral prophylaxis. HIV-1-infected and uninfected women did not show any differences in the expression of placental cytokine secretion except for a trend toward lower TNF-10 mRNA levels in HIV-1-infected women. Within the HIV-1-infected group, women who were exposed to a long duration of ZDV (>72 days) or received SD-NVP less than 5h prior to delivery, more frequently expressed detectable levels of IL-10 in their placentas (32% versus 7% (p = 0.01) and 32% versus 5% (p = 0.02), respectively). No infant was found to be HIV-1-infected. Our results showed a normalization of the placental cytokine balance in HIV-1-infected women receiving antiretroviral prophylaxis. Furthermore, the type and duration of antiretroviral prophylaxis have an impact on the placental anti-inflammatory IL-10 expression level, which may contribute to controlling HIV replication at the placental level, thus reducing MTCT of HIV-1.
Fil: Pornprasert, Sakorn. Chiang Mai University; Tailandia
Fil: Mary, Jean-Yves. Université Paris Diderot - Paris 7; Francia
Fil: Faye, Albert. Institut National de la Santé et de la Recherche Médicale; Francia
Fil: Leechanachai, Pannee. Chiang Mai University; Tailandia
Fil: Limtrakul, Aram. Health Promotion Center Region; Tailandia
Fil: Rugpao, Sungwal. Chiang Mai University; Tailandia
Fil: Sirivatanapa, Pannee. Chiang Mai University; Tailandia
Fil: Gomuthbutra, Vorapin. Nakornping Hospital; Tailandia
Fil: Matanasaravoot, Wanmanee. Lamphun Hospital; Tailandia
Fil: Le Coer, Sophie. Institut National d’Etudes Démographiques; Francia
Fil: Lallemant, Marc. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia
Fil: Barré-Sinoussi, Françoise. Instituto Pasteur; Francia
Fil: Menu, Elisabeth. Instituto Pasteur; Francia
Fil: Ngo Giang Huong, Nicole. Centre National de la Recherche Scientifique. Institut de Recherche pour le Développement; Francia
Fil: Ayouba, Ahidjo. Instituto Pasteur; Francia
Fil: Chailert, Sanupong. Chiang Mai University; Tailandia. Instituto Pasteur; Francia
Fil: Chaouat, Gérard. Institut National de la Santé et de la Recherche Médicale; Francia
Fil: Derrien, Muriel. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Dolcini, Guillermina Laura. Universidad Nacional del Centro de la Provincia de Buenos Aires. Facultad de Ciencias Veterinarias. Departamento de Sanidad Animal y Medicina Preventiva. Laboratorio de Virología; Argentina. Instituto Pasteur; Francia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigación Veterinaria de Tandil. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigación Veterinaria de Tandil. Provincia de Buenos Aires. Gobernación. Comision de Investigaciones Científicas. Centro de Investigación Veterinaria de Tandil; Argentina
Fil: Eteki, Nicole. Hôpital Central de Yaoundé. Maternité Principale; Camerún
Fil: Kfutwah, Anfumbom Jude. Instituto Pasteur; Francia
Fil: Kouo, Odette. Instituto Pasteur; Francia
Fil: Lemen, Brigitte. Instituto Pasteur; Francia
Fil: Abal, Facundo Juan Pablo. Instituto Pasteur; Francia
Fil: Nerrienet, Eric. Instituto Pasteur; Francia
Fil: Njinku, Bernadette. Instituto Pasteur; Francia
Fil: Scarlatti, Gabriella. Suan Dok Hospital; Tailandia
Fil: Tejiokem, Mathurin. Centre Pasteur du Cameroun; Camerún
Fil: Téné, Gilbert. No especifíca;