Efectos del tratamiento prolongado con AZT sobre células tumorales : acortamiento telomérico, inducción de senescencia y apoptosis, y reducción de tumorigenicidad

Autores
Tejera, Agueda Mercedes
Año de publicación
2002
Idioma
español castellano
Tipo de recurso
tesis doctoral
Estado
versión publicada
Colaborador/a o director/a de tesis
Gómez, Daniel E.
Alonso, Daniel Fernando
Descripción
Normal cells in culture divide a certain amount of times and undergo a processtermed replicative senescence. Telomere loss is thought to control entry intosenescence. Activation of telomerase in tumors bypasses cellular senescence and isthus a requirement for tumor progression. In this work, we have investigated theeffects of chronic in vitro 3'-azido-2', 3'-dideoxythymidine (AZT)exposure on humanand murine carcinoma cells. We demonstrate the irreversible telomere shortening inhuman cervical cancer cells (HeLa) cultured for long-term with AZT, but withoutevidence of senescence. Furthermore, we demonstrate, for the first time, that AZT-treatedmurine mammary carcinoma cells (F3II) have a reduced tumorigenicity insyngeneic BALB/c mice. Tumor incidence was reduced and survival was prolonged inanimals inoculated with AZT-treated cells when comparing with control counterparts. The number and size of spontaneous metastases were also decreased in animalsreceiving AZT-treated F3II cells. In addition, we present morphological and biochemicalevidence of senescence, and induction of apoptosis in tumor cells exposed to AZT. These data indicate that chronic exposure of mammary carcinoma cells to AZT may besufficient to induce a senescent phenotype and to reduce tumorigenicity.
Fil: Tejera, Agueda Mercedes. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
Materia
APOPTOSIS
AZT
CANCER DE MAMA
SENESCENCIA
TELOMERASA
APOPTOSIS
AZT
BREAST CANCER
SENESCENCE
TELOMERASE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar
Repositorio
Biblioteca Digital (UBA-FCEN)
Institución
Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
OAI Identificador
tesis:tesis_n3502_Tejera
Acceder
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oai_identifier_str tesis:tesis_n3502_Tejera
network_acronym_str BDUBAFCEN
repository_id_str 1896
network_name_str Biblioteca Digital (UBA-FCEN)
spelling Efectos del tratamiento prolongado con AZT sobre células tumorales : acortamiento telomérico, inducción de senescencia y apoptosis, y reducción de tumorigenicidadEffects of AZT prolonged treatment on tumor cells: telomere shortening, induction of senescence and apoptosis and reduction of tumorigenicityTejera, Agueda MercedesAPOPTOSISAZTCANCER DE MAMASENESCENCIATELOMERASAAPOPTOSISAZTBREAST CANCERSENESCENCETELOMERASENormal cells in culture divide a certain amount of times and undergo a processtermed replicative senescence. Telomere loss is thought to control entry intosenescence. Activation of telomerase in tumors bypasses cellular senescence and isthus a requirement for tumor progression. In this work, we have investigated theeffects of chronic in vitro 3'-azido-2', 3'-dideoxythymidine (AZT)exposure on humanand murine carcinoma cells. We demonstrate the irreversible telomere shortening inhuman cervical cancer cells (HeLa) cultured for long-term with AZT, but withoutevidence of senescence. Furthermore, we demonstrate, for the first time, that AZT-treatedmurine mammary carcinoma cells (F3II) have a reduced tumorigenicity insyngeneic BALB/c mice. Tumor incidence was reduced and survival was prolonged inanimals inoculated with AZT-treated cells when comparing with control counterparts. The number and size of spontaneous metastases were also decreased in animalsreceiving AZT-treated F3II cells. In addition, we present morphological and biochemicalevidence of senescence, and induction of apoptosis in tumor cells exposed to AZT. These data indicate that chronic exposure of mammary carcinoma cells to AZT may besufficient to induce a senescent phenotype and to reduce tumorigenicity.Fil: Tejera, Agueda Mercedes. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.Universidad de Buenos Aires. Facultad de Ciencias Exactas y NaturalesGómez, Daniel E.Alonso, Daniel Fernando2002info:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_db06info:ar-repo/semantics/tesisDoctoralapplication/pdfhttps://hdl.handle.net/20.500.12110/tesis_n3502_Tejeraspainfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/arreponame:Biblioteca Digital (UBA-FCEN)instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesinstacron:UBA-FCEN2025-10-16T09:29:04Ztesis:tesis_n3502_TejeraInstitucionalhttps://digital.bl.fcen.uba.ar/Universidad públicaNo correspondehttps://digital.bl.fcen.uba.ar/cgi-bin/oaiserver.cgiana@bl.fcen.uba.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:18962025-10-16 09:29:06.469Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturalesfalse
dc.title.none.fl_str_mv Efectos del tratamiento prolongado con AZT sobre células tumorales : acortamiento telomérico, inducción de senescencia y apoptosis, y reducción de tumorigenicidad
Effects of AZT prolonged treatment on tumor cells: telomere shortening, induction of senescence and apoptosis and reduction of tumorigenicity
title Efectos del tratamiento prolongado con AZT sobre células tumorales : acortamiento telomérico, inducción de senescencia y apoptosis, y reducción de tumorigenicidad
spellingShingle Efectos del tratamiento prolongado con AZT sobre células tumorales : acortamiento telomérico, inducción de senescencia y apoptosis, y reducción de tumorigenicidad
Tejera, Agueda Mercedes
APOPTOSIS
AZT
CANCER DE MAMA
SENESCENCIA
TELOMERASA
APOPTOSIS
AZT
BREAST CANCER
SENESCENCE
TELOMERASE
title_short Efectos del tratamiento prolongado con AZT sobre células tumorales : acortamiento telomérico, inducción de senescencia y apoptosis, y reducción de tumorigenicidad
title_full Efectos del tratamiento prolongado con AZT sobre células tumorales : acortamiento telomérico, inducción de senescencia y apoptosis, y reducción de tumorigenicidad
title_fullStr Efectos del tratamiento prolongado con AZT sobre células tumorales : acortamiento telomérico, inducción de senescencia y apoptosis, y reducción de tumorigenicidad
title_full_unstemmed Efectos del tratamiento prolongado con AZT sobre células tumorales : acortamiento telomérico, inducción de senescencia y apoptosis, y reducción de tumorigenicidad
title_sort Efectos del tratamiento prolongado con AZT sobre células tumorales : acortamiento telomérico, inducción de senescencia y apoptosis, y reducción de tumorigenicidad
dc.creator.none.fl_str_mv Tejera, Agueda Mercedes
author Tejera, Agueda Mercedes
author_facet Tejera, Agueda Mercedes
author_role author
dc.contributor.none.fl_str_mv Gómez, Daniel E.
Alonso, Daniel Fernando
dc.subject.none.fl_str_mv APOPTOSIS
AZT
CANCER DE MAMA
SENESCENCIA
TELOMERASA
APOPTOSIS
AZT
BREAST CANCER
SENESCENCE
TELOMERASE
topic APOPTOSIS
AZT
CANCER DE MAMA
SENESCENCIA
TELOMERASA
APOPTOSIS
AZT
BREAST CANCER
SENESCENCE
TELOMERASE
dc.description.none.fl_txt_mv Normal cells in culture divide a certain amount of times and undergo a processtermed replicative senescence. Telomere loss is thought to control entry intosenescence. Activation of telomerase in tumors bypasses cellular senescence and isthus a requirement for tumor progression. In this work, we have investigated theeffects of chronic in vitro 3'-azido-2', 3'-dideoxythymidine (AZT)exposure on humanand murine carcinoma cells. We demonstrate the irreversible telomere shortening inhuman cervical cancer cells (HeLa) cultured for long-term with AZT, but withoutevidence of senescence. Furthermore, we demonstrate, for the first time, that AZT-treatedmurine mammary carcinoma cells (F3II) have a reduced tumorigenicity insyngeneic BALB/c mice. Tumor incidence was reduced and survival was prolonged inanimals inoculated with AZT-treated cells when comparing with control counterparts. The number and size of spontaneous metastases were also decreased in animalsreceiving AZT-treated F3II cells. In addition, we present morphological and biochemicalevidence of senescence, and induction of apoptosis in tumor cells exposed to AZT. These data indicate that chronic exposure of mammary carcinoma cells to AZT may besufficient to induce a senescent phenotype and to reduce tumorigenicity.
Fil: Tejera, Agueda Mercedes. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina.
description Normal cells in culture divide a certain amount of times and undergo a processtermed replicative senescence. Telomere loss is thought to control entry intosenescence. Activation of telomerase in tumors bypasses cellular senescence and isthus a requirement for tumor progression. In this work, we have investigated theeffects of chronic in vitro 3'-azido-2', 3'-dideoxythymidine (AZT)exposure on humanand murine carcinoma cells. We demonstrate the irreversible telomere shortening inhuman cervical cancer cells (HeLa) cultured for long-term with AZT, but withoutevidence of senescence. Furthermore, we demonstrate, for the first time, that AZT-treatedmurine mammary carcinoma cells (F3II) have a reduced tumorigenicity insyngeneic BALB/c mice. Tumor incidence was reduced and survival was prolonged inanimals inoculated with AZT-treated cells when comparing with control counterparts. The number and size of spontaneous metastases were also decreased in animalsreceiving AZT-treated F3II cells. In addition, we present morphological and biochemicalevidence of senescence, and induction of apoptosis in tumor cells exposed to AZT. These data indicate that chronic exposure of mammary carcinoma cells to AZT may besufficient to induce a senescent phenotype and to reduce tumorigenicity.
publishDate 2002
dc.date.none.fl_str_mv 2002
dc.type.none.fl_str_mv info:eu-repo/semantics/doctoralThesis
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_db06
info:ar-repo/semantics/tesisDoctoral
format doctoralThesis
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.12110/tesis_n3502_Tejera
url https://hdl.handle.net/20.500.12110/tesis_n3502_Tejera
dc.language.none.fl_str_mv spa
language spa
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales
publisher.none.fl_str_mv Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales
dc.source.none.fl_str_mv reponame:Biblioteca Digital (UBA-FCEN)
instname:Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron:UBA-FCEN
reponame_str Biblioteca Digital (UBA-FCEN)
collection Biblioteca Digital (UBA-FCEN)
instname_str Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
instacron_str UBA-FCEN
institution UBA-FCEN
repository.name.fl_str_mv Biblioteca Digital (UBA-FCEN) - Universidad Nacional de Buenos Aires. Facultad de Ciencias Exactas y Naturales
repository.mail.fl_str_mv ana@bl.fcen.uba.ar
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score 12.712165

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