Ischemic preconditioning and tacrolimus pretreatment as strategies to attenuate intestinal ischemia-reperfusion injury in mice

Autores
Stringa, Pablo Luis; Romanin, David Emmanuel; Lausada, Natalia Raquel; Machuca, Mariana Alejandra; Raimondi, J. C.; Cabbane, A.; Rumbo, Martín; Gondolesi, Gabriel Eduardo
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The intestine is highly sensitive to ischemia-reperfusion injury (IRI), a phenomenon occurring in different intestinal diseases. Several strategies to mitigate IRI are in experimental stages; unfortunately, no consensus has been reached about the most appropriate one. We report a protocol to study ischemic preconditioning (IPC) evaluation in mice and to combine IPC and tacrolimus (TAC) pretreatment in a warm ischemia model. Mice were divided into treated (IPC, TAC, and IPC þ TAC) and untreated groups before intestinal ischemia. IPC, TAC, and IPC þ TAC groups were able to decrease postreperfusion nitrites levels (P < .05). IPC-containing groups had a major beneficial effect by preserving the integrity of the intestinal histology (P < .05) and improving animal survival (P < .002) compared with TAC alone or the untreated group. The IPC þ TAC group was the only one that showed significant improvement in lung histological analysis (P < .05). The TAC and IPC þ TAC groups down-regulated intestinal expression of interleukin (II)-6 and IL1b more than 10-fold compared with the control group. Although IPC and TAC alone reduced intestinal IRI, the used of a combined therapy produced the most significant results in all the local and distant evaluated parameters.
Fil: Stringa, Pablo Luis. Fundación Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; Argentina
Fil: Romanin, David Emmanuel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lausada, Natalia Raquel. Fundación Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; Argentina
Fil: Machuca, Mariana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; Argentina
Fil: Raimondi, J. C.. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; Argentina
Fil: Cabbane, A.. Fundación Favaloro; Argentina
Fil: Rumbo, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina
Fil: Gondolesi, Gabriel Eduardo. Fundación Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
Intestinal Transplant
Ischemia Reperfusion Injury
Ischemic Preconditioning
Tacrolimus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/23536
Acceder
id CONICETDig_b17d167113129b54743ffa66c5a86fa3
oai_identifier_str oai:ri.conicet.gov.ar:11336/23536
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Ischemic preconditioning and tacrolimus pretreatment as strategies to attenuate intestinal ischemia-reperfusion injury in miceStringa, Pablo LuisRomanin, David EmmanuelLausada, Natalia RaquelMachuca, Mariana AlejandraRaimondi, J. C.Cabbane, A.Rumbo, MartínGondolesi, Gabriel EduardoIntestinal TransplantIschemia Reperfusion InjuryIschemic PreconditioningTacrolimushttps://purl.org/becyt/ford/3.2https://purl.org/becyt/ford/3https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The intestine is highly sensitive to ischemia-reperfusion injury (IRI), a phenomenon occurring in different intestinal diseases. Several strategies to mitigate IRI are in experimental stages; unfortunately, no consensus has been reached about the most appropriate one. We report a protocol to study ischemic preconditioning (IPC) evaluation in mice and to combine IPC and tacrolimus (TAC) pretreatment in a warm ischemia model. Mice were divided into treated (IPC, TAC, and IPC þ TAC) and untreated groups before intestinal ischemia. IPC, TAC, and IPC þ TAC groups were able to decrease postreperfusion nitrites levels (P < .05). IPC-containing groups had a major beneficial effect by preserving the integrity of the intestinal histology (P < .05) and improving animal survival (P < .002) compared with TAC alone or the untreated group. The IPC þ TAC group was the only one that showed significant improvement in lung histological analysis (P < .05). The TAC and IPC þ TAC groups down-regulated intestinal expression of interleukin (II)-6 and IL1b more than 10-fold compared with the control group. Although IPC and TAC alone reduced intestinal IRI, the used of a combined therapy produced the most significant results in all the local and distant evaluated parameters.Fil: Stringa, Pablo Luis. Fundación Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; ArgentinaFil: Romanin, David Emmanuel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lausada, Natalia Raquel. Fundación Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; ArgentinaFil: Machuca, Mariana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; ArgentinaFil: Raimondi, J. C.. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; ArgentinaFil: Cabbane, A.. Fundación Favaloro; ArgentinaFil: Rumbo, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; ArgentinaFil: Gondolesi, Gabriel Eduardo. Fundación Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2013-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/23536Stringa, Pablo Luis; Romanin, David Emmanuel; Lausada, Natalia Raquel; Machuca, Mariana Alejandra; Raimondi, J. C.; et al.; Ischemic preconditioning and tacrolimus pretreatment as strategies to attenuate intestinal ischemia-reperfusion injury in mice; Elsevier; Transplantation Proceedings; 45; 6; 8-2013; 2480-24850041-1345CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0041134513004752info:eu-repo/semantics/altIdentifier/doi/10.1016/j.transproceed.2013.02.113info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-11-05T10:25:18Zoai:ri.conicet.gov.ar:11336/23536instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-11-05 10:25:19.261CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Ischemic preconditioning and tacrolimus pretreatment as strategies to attenuate intestinal ischemia-reperfusion injury in mice
title Ischemic preconditioning and tacrolimus pretreatment as strategies to attenuate intestinal ischemia-reperfusion injury in mice
spellingShingle Ischemic preconditioning and tacrolimus pretreatment as strategies to attenuate intestinal ischemia-reperfusion injury in mice
Stringa, Pablo Luis
Intestinal Transplant
Ischemia Reperfusion Injury
Ischemic Preconditioning
Tacrolimus
title_short Ischemic preconditioning and tacrolimus pretreatment as strategies to attenuate intestinal ischemia-reperfusion injury in mice
title_full Ischemic preconditioning and tacrolimus pretreatment as strategies to attenuate intestinal ischemia-reperfusion injury in mice
title_fullStr Ischemic preconditioning and tacrolimus pretreatment as strategies to attenuate intestinal ischemia-reperfusion injury in mice
title_full_unstemmed Ischemic preconditioning and tacrolimus pretreatment as strategies to attenuate intestinal ischemia-reperfusion injury in mice
title_sort Ischemic preconditioning and tacrolimus pretreatment as strategies to attenuate intestinal ischemia-reperfusion injury in mice
dc.creator.none.fl_str_mv Stringa, Pablo Luis
Romanin, David Emmanuel
Lausada, Natalia Raquel
Machuca, Mariana Alejandra
Raimondi, J. C.
Cabbane, A.
Rumbo, Martín
Gondolesi, Gabriel Eduardo
author Stringa, Pablo Luis
author_facet Stringa, Pablo Luis
Romanin, David Emmanuel
Lausada, Natalia Raquel
Machuca, Mariana Alejandra
Raimondi, J. C.
Cabbane, A.
Rumbo, Martín
Gondolesi, Gabriel Eduardo
author_role author
author2 Romanin, David Emmanuel
Lausada, Natalia Raquel
Machuca, Mariana Alejandra
Raimondi, J. C.
Cabbane, A.
Rumbo, Martín
Gondolesi, Gabriel Eduardo
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Intestinal Transplant
Ischemia Reperfusion Injury
Ischemic Preconditioning
Tacrolimus
topic Intestinal Transplant
Ischemia Reperfusion Injury
Ischemic Preconditioning
Tacrolimus
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.2
https://purl.org/becyt/ford/3
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The intestine is highly sensitive to ischemia-reperfusion injury (IRI), a phenomenon occurring in different intestinal diseases. Several strategies to mitigate IRI are in experimental stages; unfortunately, no consensus has been reached about the most appropriate one. We report a protocol to study ischemic preconditioning (IPC) evaluation in mice and to combine IPC and tacrolimus (TAC) pretreatment in a warm ischemia model. Mice were divided into treated (IPC, TAC, and IPC þ TAC) and untreated groups before intestinal ischemia. IPC, TAC, and IPC þ TAC groups were able to decrease postreperfusion nitrites levels (P < .05). IPC-containing groups had a major beneficial effect by preserving the integrity of the intestinal histology (P < .05) and improving animal survival (P < .002) compared with TAC alone or the untreated group. The IPC þ TAC group was the only one that showed significant improvement in lung histological analysis (P < .05). The TAC and IPC þ TAC groups down-regulated intestinal expression of interleukin (II)-6 and IL1b more than 10-fold compared with the control group. Although IPC and TAC alone reduced intestinal IRI, the used of a combined therapy produced the most significant results in all the local and distant evaluated parameters.
Fil: Stringa, Pablo Luis. Fundación Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; Argentina
Fil: Romanin, David Emmanuel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lausada, Natalia Raquel. Fundación Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; Argentina
Fil: Machuca, Mariana Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; Argentina
Fil: Raimondi, J. C.. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; Argentina
Fil: Cabbane, A.. Fundación Favaloro; Argentina
Fil: Rumbo, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Laboratorio de Investigaciones del Sistema Inmune; Argentina
Fil: Gondolesi, Gabriel Eduardo. Fundación Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description The intestine is highly sensitive to ischemia-reperfusion injury (IRI), a phenomenon occurring in different intestinal diseases. Several strategies to mitigate IRI are in experimental stages; unfortunately, no consensus has been reached about the most appropriate one. We report a protocol to study ischemic preconditioning (IPC) evaluation in mice and to combine IPC and tacrolimus (TAC) pretreatment in a warm ischemia model. Mice were divided into treated (IPC, TAC, and IPC þ TAC) and untreated groups before intestinal ischemia. IPC, TAC, and IPC þ TAC groups were able to decrease postreperfusion nitrites levels (P < .05). IPC-containing groups had a major beneficial effect by preserving the integrity of the intestinal histology (P < .05) and improving animal survival (P < .002) compared with TAC alone or the untreated group. The IPC þ TAC group was the only one that showed significant improvement in lung histological analysis (P < .05). The TAC and IPC þ TAC groups down-regulated intestinal expression of interleukin (II)-6 and IL1b more than 10-fold compared with the control group. Although IPC and TAC alone reduced intestinal IRI, the used of a combined therapy produced the most significant results in all the local and distant evaluated parameters.
publishDate 2013
dc.date.none.fl_str_mv 2013-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/23536
Stringa, Pablo Luis; Romanin, David Emmanuel; Lausada, Natalia Raquel; Machuca, Mariana Alejandra; Raimondi, J. C.; et al.; Ischemic preconditioning and tacrolimus pretreatment as strategies to attenuate intestinal ischemia-reperfusion injury in mice; Elsevier; Transplantation Proceedings; 45; 6; 8-2013; 2480-2485
0041-1345
CONICET Digital
CONICET
url http://hdl.handle.net/11336/23536
identifier_str_mv Stringa, Pablo Luis; Romanin, David Emmanuel; Lausada, Natalia Raquel; Machuca, Mariana Alejandra; Raimondi, J. C.; et al.; Ischemic preconditioning and tacrolimus pretreatment as strategies to attenuate intestinal ischemia-reperfusion injury in mice; Elsevier; Transplantation Proceedings; 45; 6; 8-2013; 2480-2485
0041-1345
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.sciencedirect.com/science/article/pii/S0041134513004752
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.transproceed.2013.02.113
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.087074

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