Defining the nonreturn time for intestinal ischemia reperfusion injury in mice

Autores
Stringa, Pablo Luis; Lausada, Natalia Raquel; Romanin, David Emmanuel; Machuca, Mariana Alejandra; Cabanne, Ana; Rumbo, Martín; Gondolesi, Gabriel Eduardo
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Among the abdominal organs, the intestine is probably the most sensitive to ischemia reperfusion injury (IRI), a phenomenon that occurs in many intestinal disorders. Few studies have reported in detail the impact of intestinal ischemia time in mice. We evaluated the effect of various warm intestinal ischemia times in an intestinal IRI model in mice. Adult male Balb/c mice were divided into 4 groups that differed in intestinal ischemia time: G1, 30; minutes; G2, 35 minutes; G3, 40 minutes; and G4, 45 minutes. Histological evaluation showed average Park scores as follows: G1 0.6 ± 0.55; G2 1.8 ± 0.45; G3 4.8 ± 2.25; and G4 5 ± 1.79. All animals from G1 survived 30 hours. G2 animals showed intermediate behavior with all succumbing between 18 and 30 hours postprocedure. G3 and G4 displayed similar survival results with animals succumbing before 6 hours after intestinal reperfusion. These data showed that Park index scores of 3 or higher were related to early death. We concluded that the 5 minutes between 35 and 40 minutes is the critical limit, after which all mice die after reperfusion. This result may represent a valuable tool for future research in mice.
Fil: Stringa, Pablo Luis. Universidad Favaloro; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lausada, Natalia Raquel. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; Argentina. Universidad Favaloro; Argentina
Fil: Romanin, David Emmanuel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Machuca, Mariana Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología Especial; Argentina
Fil: Cabanne, Ana. Universidad Favaloro; Argentina
Fil: Rumbo, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Gondolesi, Gabriel Eduardo. Universidad Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
INTESTINAL
ISCHEMIA REPERFUSION INJURY
MICE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/195943
Acceder
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Defining the nonreturn time for intestinal ischemia reperfusion injury in miceStringa, Pablo LuisLausada, Natalia RaquelRomanin, David EmmanuelMachuca, Mariana AlejandraCabanne, AnaRumbo, MartínGondolesi, Gabriel EduardoINTESTINALISCHEMIA REPERFUSION INJURYMICEhttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Among the abdominal organs, the intestine is probably the most sensitive to ischemia reperfusion injury (IRI), a phenomenon that occurs in many intestinal disorders. Few studies have reported in detail the impact of intestinal ischemia time in mice. We evaluated the effect of various warm intestinal ischemia times in an intestinal IRI model in mice. Adult male Balb/c mice were divided into 4 groups that differed in intestinal ischemia time: G1, 30; minutes; G2, 35 minutes; G3, 40 minutes; and G4, 45 minutes. Histological evaluation showed average Park scores as follows: G1 0.6 ± 0.55; G2 1.8 ± 0.45; G3 4.8 ± 2.25; and G4 5 ± 1.79. All animals from G1 survived 30 hours. G2 animals showed intermediate behavior with all succumbing between 18 and 30 hours postprocedure. G3 and G4 displayed similar survival results with animals succumbing before 6 hours after intestinal reperfusion. These data showed that Park index scores of 3 or higher were related to early death. We concluded that the 5 minutes between 35 and 40 minutes is the critical limit, after which all mice die after reperfusion. This result may represent a valuable tool for future research in mice.Fil: Stringa, Pablo Luis. Universidad Favaloro; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lausada, Natalia Raquel. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; Argentina. Universidad Favaloro; ArgentinaFil: Romanin, David Emmanuel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Machuca, Mariana Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología Especial; ArgentinaFil: Cabanne, Ana. Universidad Favaloro; ArgentinaFil: Rumbo, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; ArgentinaFil: Gondolesi, Gabriel Eduardo. Universidad Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier Science Inc.2012-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/195943Stringa, Pablo Luis; Lausada, Natalia Raquel; Romanin, David Emmanuel; Machuca, Mariana Alejandra; Cabanne, Ana; et al.; Defining the nonreturn time for intestinal ischemia reperfusion injury in mice; Elsevier Science Inc.; Transplantation Proceedings; 44; 5; 6-2012; 1214-12170041-1345CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.transproceed.2011.11.066info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:10:29Zoai:ri.conicet.gov.ar:11336/195943instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:10:29.916CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Defining the nonreturn time for intestinal ischemia reperfusion injury in mice
title Defining the nonreturn time for intestinal ischemia reperfusion injury in mice
spellingShingle Defining the nonreturn time for intestinal ischemia reperfusion injury in mice
Stringa, Pablo Luis
INTESTINAL
ISCHEMIA REPERFUSION INJURY
MICE
title_short Defining the nonreturn time for intestinal ischemia reperfusion injury in mice
title_full Defining the nonreturn time for intestinal ischemia reperfusion injury in mice
title_fullStr Defining the nonreturn time for intestinal ischemia reperfusion injury in mice
title_full_unstemmed Defining the nonreturn time for intestinal ischemia reperfusion injury in mice
title_sort Defining the nonreturn time for intestinal ischemia reperfusion injury in mice
dc.creator.none.fl_str_mv Stringa, Pablo Luis
Lausada, Natalia Raquel
Romanin, David Emmanuel
Machuca, Mariana Alejandra
Cabanne, Ana
Rumbo, Martín
Gondolesi, Gabriel Eduardo
author Stringa, Pablo Luis
author_facet Stringa, Pablo Luis
Lausada, Natalia Raquel
Romanin, David Emmanuel
Machuca, Mariana Alejandra
Cabanne, Ana
Rumbo, Martín
Gondolesi, Gabriel Eduardo
author_role author
author2 Lausada, Natalia Raquel
Romanin, David Emmanuel
Machuca, Mariana Alejandra
Cabanne, Ana
Rumbo, Martín
Gondolesi, Gabriel Eduardo
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv INTESTINAL
ISCHEMIA REPERFUSION INJURY
MICE
topic INTESTINAL
ISCHEMIA REPERFUSION INJURY
MICE
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Among the abdominal organs, the intestine is probably the most sensitive to ischemia reperfusion injury (IRI), a phenomenon that occurs in many intestinal disorders. Few studies have reported in detail the impact of intestinal ischemia time in mice. We evaluated the effect of various warm intestinal ischemia times in an intestinal IRI model in mice. Adult male Balb/c mice were divided into 4 groups that differed in intestinal ischemia time: G1, 30; minutes; G2, 35 minutes; G3, 40 minutes; and G4, 45 minutes. Histological evaluation showed average Park scores as follows: G1 0.6 ± 0.55; G2 1.8 ± 0.45; G3 4.8 ± 2.25; and G4 5 ± 1.79. All animals from G1 survived 30 hours. G2 animals showed intermediate behavior with all succumbing between 18 and 30 hours postprocedure. G3 and G4 displayed similar survival results with animals succumbing before 6 hours after intestinal reperfusion. These data showed that Park index scores of 3 or higher were related to early death. We concluded that the 5 minutes between 35 and 40 minutes is the critical limit, after which all mice die after reperfusion. This result may represent a valuable tool for future research in mice.
Fil: Stringa, Pablo Luis. Universidad Favaloro; Argentina. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lausada, Natalia Raquel. Universidad Nacional de la Plata. Facultad de Ciencias Médicas. Laboratorio de Transplante de Órganos; Argentina. Universidad Favaloro; Argentina
Fil: Romanin, David Emmanuel. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Machuca, Mariana Alejandra. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias. Cátedra de Patología Especial; Argentina
Fil: Cabanne, Ana. Universidad Favaloro; Argentina
Fil: Rumbo, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas; Argentina
Fil: Gondolesi, Gabriel Eduardo. Universidad Favaloro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Among the abdominal organs, the intestine is probably the most sensitive to ischemia reperfusion injury (IRI), a phenomenon that occurs in many intestinal disorders. Few studies have reported in detail the impact of intestinal ischemia time in mice. We evaluated the effect of various warm intestinal ischemia times in an intestinal IRI model in mice. Adult male Balb/c mice were divided into 4 groups that differed in intestinal ischemia time: G1, 30; minutes; G2, 35 minutes; G3, 40 minutes; and G4, 45 minutes. Histological evaluation showed average Park scores as follows: G1 0.6 ± 0.55; G2 1.8 ± 0.45; G3 4.8 ± 2.25; and G4 5 ± 1.79. All animals from G1 survived 30 hours. G2 animals showed intermediate behavior with all succumbing between 18 and 30 hours postprocedure. G3 and G4 displayed similar survival results with animals succumbing before 6 hours after intestinal reperfusion. These data showed that Park index scores of 3 or higher were related to early death. We concluded that the 5 minutes between 35 and 40 minutes is the critical limit, after which all mice die after reperfusion. This result may represent a valuable tool for future research in mice.
publishDate 2012
dc.date.none.fl_str_mv 2012-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/195943
Stringa, Pablo Luis; Lausada, Natalia Raquel; Romanin, David Emmanuel; Machuca, Mariana Alejandra; Cabanne, Ana; et al.; Defining the nonreturn time for intestinal ischemia reperfusion injury in mice; Elsevier Science Inc.; Transplantation Proceedings; 44; 5; 6-2012; 1214-1217
0041-1345
CONICET Digital
CONICET
url http://hdl.handle.net/11336/195943
identifier_str_mv Stringa, Pablo Luis; Lausada, Natalia Raquel; Romanin, David Emmanuel; Machuca, Mariana Alejandra; Cabanne, Ana; et al.; Defining the nonreturn time for intestinal ischemia reperfusion injury in mice; Elsevier Science Inc.; Transplantation Proceedings; 44; 5; 6-2012; 1214-1217
0041-1345
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.transproceed.2011.11.066
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc.
publisher.none.fl_str_mv Elsevier Science Inc.
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842270121893036032
score 13.13397

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