Galactomannan as a potential modulator of intestinal ischemia–reperfusion injury

Autores
Stringa, Pablo; Toledano, Victor; Papa Gobbi, Rodrigo; Arreola, Miguel; Largo, Carlota; Machuca, Mariana; Aguirre, Luis A.; Rumbo, Martín; López Collazo, Eduardo; Hernández Oliveros, Francisco
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Galactomannan (GAL), a polysaccharide present on the cell wall of several fungi, has shown an ability to modulate inflammatory responses through the dectin-1 receptor in human macrophages. However, studies evaluating the modulatory properties of this polysaccharide in in vivo inflammatory scenarios are scarce. We hypothesized that GAL pretreatment would modulate local and remote damage related to intestinal reperfusion after an ischemic insult. Materials and methods: Adult male Balb/c mice were subjected to intestinal ischemia–reperfusion injury by reversible occlusion of the superior mesenteric artery, consisting of 45 min of ischemia followed by 3 or 24 h of reperfusion. Intragastric GAL (70 mg/kg) was administered 12 h before ischemia, and saline solution was used in the control animals. Jejunum, lung, and blood samples were taken for the analysis of histology, gene expression, plasma cytokine levels, and nitrosative stress. Results: Intestinal and lung histologic alterations were attenuated by GAL pretreatment, showing significant differences compared with nontreated animals. Interleukin 1β, monocyte chemoattractant protein 1, and IL-6 messenger RNA expression were considerably downregulated in the small intestine of the GAL group. In addition, GAL treatment significantly prevented plasma interleukin 6 and monocyte chemoattractant protein 1 upregulation and diminished nitrate and nitrite levels after 3 h of intestinal reperfusion. Conclusions: GAL pretreatment constitutes a novel and promising therapy to reduce local and remote damage triggered by intestinal ischemia–reperfusion injury. Further in vivo and in vitro studies to understand GAL's modulatory effects are warranted.
Fil: Stringa, Pablo. Hospital Universitario la Paz; España
Fil: Toledano, Victor. Hospital Universitario la Paz; España
Fil: Papa Gobbi, Rodrigo. Hospital Universitario la Paz; España
Fil: Arreola, Miguel. Hospital Universitario la Paz; España
Fil: Largo, Carlota. Hospital Universitario la Paz; España
Fil: Machuca, Mariana. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Aguirre, Luis A.. Hospital Universitario la Paz; España
Fil: Rumbo, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
Fil: López Collazo, Eduardo. Hospital Universitario la Paz; España
Fil: Hernández Oliveros, Francisco. Hospital Universitario la Paz; España
Materia
DIGESTIVE SYSTEM SURGICAL PROCEDURES
INTESTINE
MESENTERIC ISCHEMIA
REPERFUSION INJURY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/151984
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/151984
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Galactomannan as a potential modulator of intestinal ischemia–reperfusion injuryStringa, PabloToledano, VictorPapa Gobbi, RodrigoArreola, MiguelLargo, CarlotaMachuca, MarianaAguirre, Luis A.Rumbo, MartínLópez Collazo, EduardoHernández Oliveros, FranciscoDIGESTIVE SYSTEM SURGICAL PROCEDURESINTESTINEMESENTERIC ISCHEMIAREPERFUSION INJURYhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Background: Galactomannan (GAL), a polysaccharide present on the cell wall of several fungi, has shown an ability to modulate inflammatory responses through the dectin-1 receptor in human macrophages. However, studies evaluating the modulatory properties of this polysaccharide in in vivo inflammatory scenarios are scarce. We hypothesized that GAL pretreatment would modulate local and remote damage related to intestinal reperfusion after an ischemic insult. Materials and methods: Adult male Balb/c mice were subjected to intestinal ischemia–reperfusion injury by reversible occlusion of the superior mesenteric artery, consisting of 45 min of ischemia followed by 3 or 24 h of reperfusion. Intragastric GAL (70 mg/kg) was administered 12 h before ischemia, and saline solution was used in the control animals. Jejunum, lung, and blood samples were taken for the analysis of histology, gene expression, plasma cytokine levels, and nitrosative stress. Results: Intestinal and lung histologic alterations were attenuated by GAL pretreatment, showing significant differences compared with nontreated animals. Interleukin 1β, monocyte chemoattractant protein 1, and IL-6 messenger RNA expression were considerably downregulated in the small intestine of the GAL group. In addition, GAL treatment significantly prevented plasma interleukin 6 and monocyte chemoattractant protein 1 upregulation and diminished nitrate and nitrite levels after 3 h of intestinal reperfusion. Conclusions: GAL pretreatment constitutes a novel and promising therapy to reduce local and remote damage triggered by intestinal ischemia–reperfusion injury. Further in vivo and in vitro studies to understand GAL's modulatory effects are warranted.Fil: Stringa, Pablo. Hospital Universitario la Paz; EspañaFil: Toledano, Victor. Hospital Universitario la Paz; EspañaFil: Papa Gobbi, Rodrigo. Hospital Universitario la Paz; EspañaFil: Arreola, Miguel. Hospital Universitario la Paz; EspañaFil: Largo, Carlota. Hospital Universitario la Paz; EspañaFil: Machuca, Mariana. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; ArgentinaFil: Aguirre, Luis A.. Hospital Universitario la Paz; EspañaFil: Rumbo, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; ArgentinaFil: López Collazo, Eduardo. Hospital Universitario la Paz; EspañaFil: Hernández Oliveros, Francisco. Hospital Universitario la Paz; EspañaAcademic Press Inc Elsevier Science2020-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/151984Stringa, Pablo; Toledano, Victor; Papa Gobbi, Rodrigo; Arreola, Miguel; Largo, Carlota; et al.; Galactomannan as a potential modulator of intestinal ischemia–reperfusion injury; Academic Press Inc Elsevier Science; Journal Of Surgical Research; 249; 01-5-2020; 232-2400022-48041095-8673CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0022480419307565info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jss.2019.10.027info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:02:27Zoai:ri.conicet.gov.ar:11336/151984instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:02:27.878CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Galactomannan as a potential modulator of intestinal ischemia–reperfusion injury
title Galactomannan as a potential modulator of intestinal ischemia–reperfusion injury
spellingShingle Galactomannan as a potential modulator of intestinal ischemia–reperfusion injury
Stringa, Pablo
DIGESTIVE SYSTEM SURGICAL PROCEDURES
INTESTINE
MESENTERIC ISCHEMIA
REPERFUSION INJURY
title_short Galactomannan as a potential modulator of intestinal ischemia–reperfusion injury
title_full Galactomannan as a potential modulator of intestinal ischemia–reperfusion injury
title_fullStr Galactomannan as a potential modulator of intestinal ischemia–reperfusion injury
title_full_unstemmed Galactomannan as a potential modulator of intestinal ischemia–reperfusion injury
title_sort Galactomannan as a potential modulator of intestinal ischemia–reperfusion injury
dc.creator.none.fl_str_mv Stringa, Pablo
Toledano, Victor
Papa Gobbi, Rodrigo
Arreola, Miguel
Largo, Carlota
Machuca, Mariana
Aguirre, Luis A.
Rumbo, Martín
López Collazo, Eduardo
Hernández Oliveros, Francisco
author Stringa, Pablo
author_facet Stringa, Pablo
Toledano, Victor
Papa Gobbi, Rodrigo
Arreola, Miguel
Largo, Carlota
Machuca, Mariana
Aguirre, Luis A.
Rumbo, Martín
López Collazo, Eduardo
Hernández Oliveros, Francisco
author_role author
author2 Toledano, Victor
Papa Gobbi, Rodrigo
Arreola, Miguel
Largo, Carlota
Machuca, Mariana
Aguirre, Luis A.
Rumbo, Martín
López Collazo, Eduardo
Hernández Oliveros, Francisco
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv DIGESTIVE SYSTEM SURGICAL PROCEDURES
INTESTINE
MESENTERIC ISCHEMIA
REPERFUSION INJURY
topic DIGESTIVE SYSTEM SURGICAL PROCEDURES
INTESTINE
MESENTERIC ISCHEMIA
REPERFUSION INJURY
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: Galactomannan (GAL), a polysaccharide present on the cell wall of several fungi, has shown an ability to modulate inflammatory responses through the dectin-1 receptor in human macrophages. However, studies evaluating the modulatory properties of this polysaccharide in in vivo inflammatory scenarios are scarce. We hypothesized that GAL pretreatment would modulate local and remote damage related to intestinal reperfusion after an ischemic insult. Materials and methods: Adult male Balb/c mice were subjected to intestinal ischemia–reperfusion injury by reversible occlusion of the superior mesenteric artery, consisting of 45 min of ischemia followed by 3 or 24 h of reperfusion. Intragastric GAL (70 mg/kg) was administered 12 h before ischemia, and saline solution was used in the control animals. Jejunum, lung, and blood samples were taken for the analysis of histology, gene expression, plasma cytokine levels, and nitrosative stress. Results: Intestinal and lung histologic alterations were attenuated by GAL pretreatment, showing significant differences compared with nontreated animals. Interleukin 1β, monocyte chemoattractant protein 1, and IL-6 messenger RNA expression were considerably downregulated in the small intestine of the GAL group. In addition, GAL treatment significantly prevented plasma interleukin 6 and monocyte chemoattractant protein 1 upregulation and diminished nitrate and nitrite levels after 3 h of intestinal reperfusion. Conclusions: GAL pretreatment constitutes a novel and promising therapy to reduce local and remote damage triggered by intestinal ischemia–reperfusion injury. Further in vivo and in vitro studies to understand GAL's modulatory effects are warranted.
Fil: Stringa, Pablo. Hospital Universitario la Paz; España
Fil: Toledano, Victor. Hospital Universitario la Paz; España
Fil: Papa Gobbi, Rodrigo. Hospital Universitario la Paz; España
Fil: Arreola, Miguel. Hospital Universitario la Paz; España
Fil: Largo, Carlota. Hospital Universitario la Paz; España
Fil: Machuca, Mariana. Universidad Nacional de La Plata. Facultad de Ciencias Veterinarias; Argentina
Fil: Aguirre, Luis A.. Hospital Universitario la Paz; España
Fil: Rumbo, Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Estudios Inmunológicos y Fisiopatológicos. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Instituto de Estudios Inmunológicos y Fisiopatológicos; Argentina
Fil: López Collazo, Eduardo. Hospital Universitario la Paz; España
Fil: Hernández Oliveros, Francisco. Hospital Universitario la Paz; España
description Background: Galactomannan (GAL), a polysaccharide present on the cell wall of several fungi, has shown an ability to modulate inflammatory responses through the dectin-1 receptor in human macrophages. However, studies evaluating the modulatory properties of this polysaccharide in in vivo inflammatory scenarios are scarce. We hypothesized that GAL pretreatment would modulate local and remote damage related to intestinal reperfusion after an ischemic insult. Materials and methods: Adult male Balb/c mice were subjected to intestinal ischemia–reperfusion injury by reversible occlusion of the superior mesenteric artery, consisting of 45 min of ischemia followed by 3 or 24 h of reperfusion. Intragastric GAL (70 mg/kg) was administered 12 h before ischemia, and saline solution was used in the control animals. Jejunum, lung, and blood samples were taken for the analysis of histology, gene expression, plasma cytokine levels, and nitrosative stress. Results: Intestinal and lung histologic alterations were attenuated by GAL pretreatment, showing significant differences compared with nontreated animals. Interleukin 1β, monocyte chemoattractant protein 1, and IL-6 messenger RNA expression were considerably downregulated in the small intestine of the GAL group. In addition, GAL treatment significantly prevented plasma interleukin 6 and monocyte chemoattractant protein 1 upregulation and diminished nitrate and nitrite levels after 3 h of intestinal reperfusion. Conclusions: GAL pretreatment constitutes a novel and promising therapy to reduce local and remote damage triggered by intestinal ischemia–reperfusion injury. Further in vivo and in vitro studies to understand GAL's modulatory effects are warranted.
publishDate 2020
dc.date.none.fl_str_mv 2020-05-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/151984
Stringa, Pablo; Toledano, Victor; Papa Gobbi, Rodrigo; Arreola, Miguel; Largo, Carlota; et al.; Galactomannan as a potential modulator of intestinal ischemia–reperfusion injury; Academic Press Inc Elsevier Science; Journal Of Surgical Research; 249; 01-5-2020; 232-240
0022-4804
1095-8673
CONICET Digital
CONICET
url http://hdl.handle.net/11336/151984
identifier_str_mv Stringa, Pablo; Toledano, Victor; Papa Gobbi, Rodrigo; Arreola, Miguel; Largo, Carlota; et al.; Galactomannan as a potential modulator of intestinal ischemia–reperfusion injury; Academic Press Inc Elsevier Science; Journal Of Surgical Research; 249; 01-5-2020; 232-240
0022-4804
1095-8673
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0022480419307565
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.jss.2019.10.027
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Academic Press Inc Elsevier Science
publisher.none.fl_str_mv Academic Press Inc Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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