Donor preconditioning with rabbit anti-rat thymocyte immunoglobulin ameliorates ischemia reperfusion injury in rat kidney transplantation

Autores
Cicora, Federico; Roberti, Javier Eugenio; Lausada, Natalia Raquel; González, Pedro Horacio; Guerrieri, Diego; Stringa, Pablo Luis; Cicora, Paola; Vásquez, Daniela; González, Ivana; Palti, Gustavo; Intile, Dante; Raimondi, Clemente
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
A major concern in transplantation is the preservation of organ function. Ischemia time and microcirculatory disturbance of the organ cannot be avoided and may result in ischemia reperfusion injury (IRI), increasing the risk of delayed graft function (DGF) and acute and chronic rejection. Anti-thymocyte immunoglobulin (rATG) is a polyclonal antibody preparation with multiple effects when administered to recipients. Our objective has been to evaluate whether the administration of rATG to kidney donors instead of recipients, in an experimental model of syngeneic rat transplantation, ameliorates IRI and facilitates immediate graft function recovery. Urea and creatinine levels and necrosis severity scores were significantly lower in kidneys from donors that had received rATG (urea: control: 211 ± 8. mg/dl vs. treatment: 110 ± 15. mg/dl, p < 0.001; creatinine: control: 4.6 ± 0.24. mg/dl vs. treatment: 2.6 ± 0.22. mg/dl, p < 0.001; necrosis severity scores: control: 2.3 vs. treatment: 1.6, p < 0.05). TUNEL staining showed 80 ± 13 positive cells in control group and 9 ± 3 (p < 0.001) in treatment group. In situ expression of proinflammatory cytokines TNF-α, IL-6, IL-21 and TGF-β1 was reduced in rATG group (p < 0.01); the same was observed for KIM-1 and caspase 8 (p < 0.001). Cytoprotective genes Bcl2 and HO-1 were upregulated in situ in treatment group (p < 0.001). In situ expression of IL-17, caspase 9, IL-23a, CxCl3 and ICAM1 showed no difference between groups (p > 0.05). Findings suggest ATG administered to donors may ameliorate the IRI process in kidney transplantation, expressed by lower necrosis and apoptosis scores and the improvement of renal function, which may be explained through the diminished in situ expression of inflammatory mediators.
Fil: Cicora, Federico. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina. Hospital Aleman; Argentina
Fil: Roberti, Javier Eugenio. Universidad de Belgrano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lausada, Natalia Raquel. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Fil: González, Pedro Horacio. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Guerrieri, Diego. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Stringa, Pablo Luis. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cicora, Paola. Fundación Para la Investigación y Asistencia de la Enfermedad Renal; Argentina
Fil: Vásquez, Daniela. Fundación Para la Investigación y Asistencia de la Enfermedad Renal; Argentina
Fil: González, Ivana. Fundación Para la Investigación y Asistencia de la Enfermedad Renal; Argentina
Fil: Palti, Gustavo. Hospital Alemán; Argentina
Fil: Intile, Dante. Fundación Para la Investigación y Asistencia de la Enfermedad Renal; Argentina
Fil: Raimondi, Clemente. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Materia
KIDNEY TRANSPLANTATION
DONOR PRECONDITIONING
APOPTOSIS
ISCHEMIA REPERFUSION INJURY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/245828
Acceder
id CONICETDig_28cb45c815820238f4b11b96fefa52d0
oai_identifier_str oai:ri.conicet.gov.ar:11336/245828
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Donor preconditioning with rabbit anti-rat thymocyte immunoglobulin ameliorates ischemia reperfusion injury in rat kidney transplantationCicora, FedericoRoberti, Javier EugenioLausada, Natalia RaquelGonzález, Pedro HoracioGuerrieri, DiegoStringa, Pablo LuisCicora, PaolaVásquez, DanielaGonzález, IvanaPalti, GustavoIntile, DanteRaimondi, ClementeKIDNEY TRANSPLANTATIONDONOR PRECONDITIONINGAPOPTOSISISCHEMIA REPERFUSION INJURYhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3A major concern in transplantation is the preservation of organ function. Ischemia time and microcirculatory disturbance of the organ cannot be avoided and may result in ischemia reperfusion injury (IRI), increasing the risk of delayed graft function (DGF) and acute and chronic rejection. Anti-thymocyte immunoglobulin (rATG) is a polyclonal antibody preparation with multiple effects when administered to recipients. Our objective has been to evaluate whether the administration of rATG to kidney donors instead of recipients, in an experimental model of syngeneic rat transplantation, ameliorates IRI and facilitates immediate graft function recovery. Urea and creatinine levels and necrosis severity scores were significantly lower in kidneys from donors that had received rATG (urea: control: 211 ± 8. mg/dl vs. treatment: 110 ± 15. mg/dl, p < 0.001; creatinine: control: 4.6 ± 0.24. mg/dl vs. treatment: 2.6 ± 0.22. mg/dl, p < 0.001; necrosis severity scores: control: 2.3 vs. treatment: 1.6, p < 0.05). TUNEL staining showed 80 ± 13 positive cells in control group and 9 ± 3 (p < 0.001) in treatment group. In situ expression of proinflammatory cytokines TNF-α, IL-6, IL-21 and TGF-β1 was reduced in rATG group (p < 0.01); the same was observed for KIM-1 and caspase 8 (p < 0.001). Cytoprotective genes Bcl2 and HO-1 were upregulated in situ in treatment group (p < 0.001). In situ expression of IL-17, caspase 9, IL-23a, CxCl3 and ICAM1 showed no difference between groups (p > 0.05). Findings suggest ATG administered to donors may ameliorate the IRI process in kidney transplantation, expressed by lower necrosis and apoptosis scores and the improvement of renal function, which may be explained through the diminished in situ expression of inflammatory mediators.Fil: Cicora, Federico. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina. Hospital Aleman; ArgentinaFil: Roberti, Javier Eugenio. Universidad de Belgrano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lausada, Natalia Raquel. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; ArgentinaFil: González, Pedro Horacio. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; ArgentinaFil: Guerrieri, Diego. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Stringa, Pablo Luis. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cicora, Paola. Fundación Para la Investigación y Asistencia de la Enfermedad Renal; ArgentinaFil: Vásquez, Daniela. Fundación Para la Investigación y Asistencia de la Enfermedad Renal; ArgentinaFil: González, Ivana. Fundación Para la Investigación y Asistencia de la Enfermedad Renal; ArgentinaFil: Palti, Gustavo. Hospital Alemán; ArgentinaFil: Intile, Dante. Fundación Para la Investigación y Asistencia de la Enfermedad Renal; ArgentinaFil: Raimondi, Clemente. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; ArgentinaElsevier Science2012-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/245828Cicora, Federico; Roberti, Javier Eugenio; Lausada, Natalia Raquel; González, Pedro Horacio; Guerrieri, Diego; et al.; Donor preconditioning with rabbit anti-rat thymocyte immunoglobulin ameliorates ischemia reperfusion injury in rat kidney transplantation; Elsevier Science; Transplant Immunology; 27; 1; 8-2012; 1-70966-3274CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0966327412000275info:eu-repo/semantics/altIdentifier/doi/10.1016/j.trim.2012.03.004info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:11Zoai:ri.conicet.gov.ar:11336/245828instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:11.82CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Donor preconditioning with rabbit anti-rat thymocyte immunoglobulin ameliorates ischemia reperfusion injury in rat kidney transplantation
title Donor preconditioning with rabbit anti-rat thymocyte immunoglobulin ameliorates ischemia reperfusion injury in rat kidney transplantation
spellingShingle Donor preconditioning with rabbit anti-rat thymocyte immunoglobulin ameliorates ischemia reperfusion injury in rat kidney transplantation
Cicora, Federico
KIDNEY TRANSPLANTATION
DONOR PRECONDITIONING
APOPTOSIS
ISCHEMIA REPERFUSION INJURY
title_short Donor preconditioning with rabbit anti-rat thymocyte immunoglobulin ameliorates ischemia reperfusion injury in rat kidney transplantation
title_full Donor preconditioning with rabbit anti-rat thymocyte immunoglobulin ameliorates ischemia reperfusion injury in rat kidney transplantation
title_fullStr Donor preconditioning with rabbit anti-rat thymocyte immunoglobulin ameliorates ischemia reperfusion injury in rat kidney transplantation
title_full_unstemmed Donor preconditioning with rabbit anti-rat thymocyte immunoglobulin ameliorates ischemia reperfusion injury in rat kidney transplantation
title_sort Donor preconditioning with rabbit anti-rat thymocyte immunoglobulin ameliorates ischemia reperfusion injury in rat kidney transplantation
dc.creator.none.fl_str_mv Cicora, Federico
Roberti, Javier Eugenio
Lausada, Natalia Raquel
González, Pedro Horacio
Guerrieri, Diego
Stringa, Pablo Luis
Cicora, Paola
Vásquez, Daniela
González, Ivana
Palti, Gustavo
Intile, Dante
Raimondi, Clemente
author Cicora, Federico
author_facet Cicora, Federico
Roberti, Javier Eugenio
Lausada, Natalia Raquel
González, Pedro Horacio
Guerrieri, Diego
Stringa, Pablo Luis
Cicora, Paola
Vásquez, Daniela
González, Ivana
Palti, Gustavo
Intile, Dante
Raimondi, Clemente
author_role author
author2 Roberti, Javier Eugenio
Lausada, Natalia Raquel
González, Pedro Horacio
Guerrieri, Diego
Stringa, Pablo Luis
Cicora, Paola
Vásquez, Daniela
González, Ivana
Palti, Gustavo
Intile, Dante
Raimondi, Clemente
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv KIDNEY TRANSPLANTATION
DONOR PRECONDITIONING
APOPTOSIS
ISCHEMIA REPERFUSION INJURY
topic KIDNEY TRANSPLANTATION
DONOR PRECONDITIONING
APOPTOSIS
ISCHEMIA REPERFUSION INJURY
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv A major concern in transplantation is the preservation of organ function. Ischemia time and microcirculatory disturbance of the organ cannot be avoided and may result in ischemia reperfusion injury (IRI), increasing the risk of delayed graft function (DGF) and acute and chronic rejection. Anti-thymocyte immunoglobulin (rATG) is a polyclonal antibody preparation with multiple effects when administered to recipients. Our objective has been to evaluate whether the administration of rATG to kidney donors instead of recipients, in an experimental model of syngeneic rat transplantation, ameliorates IRI and facilitates immediate graft function recovery. Urea and creatinine levels and necrosis severity scores were significantly lower in kidneys from donors that had received rATG (urea: control: 211 ± 8. mg/dl vs. treatment: 110 ± 15. mg/dl, p < 0.001; creatinine: control: 4.6 ± 0.24. mg/dl vs. treatment: 2.6 ± 0.22. mg/dl, p < 0.001; necrosis severity scores: control: 2.3 vs. treatment: 1.6, p < 0.05). TUNEL staining showed 80 ± 13 positive cells in control group and 9 ± 3 (p < 0.001) in treatment group. In situ expression of proinflammatory cytokines TNF-α, IL-6, IL-21 and TGF-β1 was reduced in rATG group (p < 0.01); the same was observed for KIM-1 and caspase 8 (p < 0.001). Cytoprotective genes Bcl2 and HO-1 were upregulated in situ in treatment group (p < 0.001). In situ expression of IL-17, caspase 9, IL-23a, CxCl3 and ICAM1 showed no difference between groups (p > 0.05). Findings suggest ATG administered to donors may ameliorate the IRI process in kidney transplantation, expressed by lower necrosis and apoptosis scores and the improvement of renal function, which may be explained through the diminished in situ expression of inflammatory mediators.
Fil: Cicora, Federico. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina. Hospital Aleman; Argentina
Fil: Roberti, Javier Eugenio. Universidad de Belgrano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lausada, Natalia Raquel. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
Fil: González, Pedro Horacio. Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas; Argentina
Fil: Guerrieri, Diego. Universidad de Buenos Aires; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Stringa, Pablo Luis. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cicora, Paola. Fundación Para la Investigación y Asistencia de la Enfermedad Renal; Argentina
Fil: Vásquez, Daniela. Fundación Para la Investigación y Asistencia de la Enfermedad Renal; Argentina
Fil: González, Ivana. Fundación Para la Investigación y Asistencia de la Enfermedad Renal; Argentina
Fil: Palti, Gustavo. Hospital Alemán; Argentina
Fil: Intile, Dante. Fundación Para la Investigación y Asistencia de la Enfermedad Renal; Argentina
Fil: Raimondi, Clemente. Universidad Nacional de La Plata. Facultad de Ciencias Médicas; Argentina
description A major concern in transplantation is the preservation of organ function. Ischemia time and microcirculatory disturbance of the organ cannot be avoided and may result in ischemia reperfusion injury (IRI), increasing the risk of delayed graft function (DGF) and acute and chronic rejection. Anti-thymocyte immunoglobulin (rATG) is a polyclonal antibody preparation with multiple effects when administered to recipients. Our objective has been to evaluate whether the administration of rATG to kidney donors instead of recipients, in an experimental model of syngeneic rat transplantation, ameliorates IRI and facilitates immediate graft function recovery. Urea and creatinine levels and necrosis severity scores were significantly lower in kidneys from donors that had received rATG (urea: control: 211 ± 8. mg/dl vs. treatment: 110 ± 15. mg/dl, p < 0.001; creatinine: control: 4.6 ± 0.24. mg/dl vs. treatment: 2.6 ± 0.22. mg/dl, p < 0.001; necrosis severity scores: control: 2.3 vs. treatment: 1.6, p < 0.05). TUNEL staining showed 80 ± 13 positive cells in control group and 9 ± 3 (p < 0.001) in treatment group. In situ expression of proinflammatory cytokines TNF-α, IL-6, IL-21 and TGF-β1 was reduced in rATG group (p < 0.01); the same was observed for KIM-1 and caspase 8 (p < 0.001). Cytoprotective genes Bcl2 and HO-1 were upregulated in situ in treatment group (p < 0.001). In situ expression of IL-17, caspase 9, IL-23a, CxCl3 and ICAM1 showed no difference between groups (p > 0.05). Findings suggest ATG administered to donors may ameliorate the IRI process in kidney transplantation, expressed by lower necrosis and apoptosis scores and the improvement of renal function, which may be explained through the diminished in situ expression of inflammatory mediators.
publishDate 2012
dc.date.none.fl_str_mv 2012-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/245828
Cicora, Federico; Roberti, Javier Eugenio; Lausada, Natalia Raquel; González, Pedro Horacio; Guerrieri, Diego; et al.; Donor preconditioning with rabbit anti-rat thymocyte immunoglobulin ameliorates ischemia reperfusion injury in rat kidney transplantation; Elsevier Science; Transplant Immunology; 27; 1; 8-2012; 1-7
0966-3274
CONICET Digital
CONICET
url http://hdl.handle.net/11336/245828
identifier_str_mv Cicora, Federico; Roberti, Javier Eugenio; Lausada, Natalia Raquel; González, Pedro Horacio; Guerrieri, Diego; et al.; Donor preconditioning with rabbit anti-rat thymocyte immunoglobulin ameliorates ischemia reperfusion injury in rat kidney transplantation; Elsevier Science; Transplant Immunology; 27; 1; 8-2012; 1-7
0966-3274
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/abs/pii/S0966327412000275
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.trim.2012.03.004
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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