Chronic postnatal stress induces voluntary alcohol intake and modifies glutamate transporters in adolescent rats

Autores
Odeon, Maria Mercedes; Andreu, Marcela; Yamauchi, Laura; Grosman, Mauricio; Acosta, Gabriela Beatriz
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Postnatal stress alters stress responses for life, with serious consequences on the central nervous system (CNS), involving glutamatergic neurotransmission and development of alcohol voluntary intake. Several drugs of abuse, including alcohol and cocaine, alter glutamate transport (GluT). Here, we evaluated effects of chronic postnatal stress (CPS) on alcohol intake and brain glutamate uptake and transporters in male adolescent Wistar rats. For CPS from postnatal day (PD) 7, the pups were separated from their mothers and exposed to cold stress (4ºC) for 1h during 20 days; controls remained with their mothers. Then they were exposed to either voluntary ethanol (6%) or dextrose (1%) intake for 7 days (5-7 rats per group), then killed. CPS: 1) increased voluntary ethanol intake; 2) did not affect body weight gain or produce signs of toxicity with alcohol exposure; 3) increased glutamate uptake by hippocampal synaptosomes in vitro 4) reduced protein levels (Western measurements) in hippocampus and frontal cortex of glial glutamate transporter-1 (GLT-1) and excitatory amino-acid transporter-3 (EAAT-3) but increased glutamate aspartate transporter (GLAST) levels. We propose that CPS-induced decrements in GLT-1 and EAAT-3 expression levels are opposed by activation of a compensatory mechanism to prevent excitotoxicity. A greater role for GLAST in total glutamate uptake to put a stop to enlarged extracellular glutamate levels is inferred. The results also demonstrate that CPS strongly increased intake of ethanol, which had little impact on effects of CPS on brain glutamate uptake or transporters. However, the impact of early life adverse events on glutamatergic neurotransmission may underlie increased alcohol consumption in adulthood.
Fil: Odeon, Maria Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Andreu, Marcela. Laboratorio Bioquímica Médica; Argentina
Fil: Yamauchi, Laura. Laboratorio Bioquímica Médica; Argentina
Fil: Grosman, Mauricio. Laboratorio Bioquímica Médica; Argentina
Fil: Acosta, Gabriela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Materia
Alcohol
Frontal Cortex
Glutamate Transporter
Glutamate Uptake
Hippocampus
Postnatal Stress
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13597
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/13597
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Chronic postnatal stress induces voluntary alcohol intake and modifies glutamate transporters in adolescent ratsOdeon, Maria MercedesAndreu, MarcelaYamauchi, LauraGrosman, MauricioAcosta, Gabriela BeatrizAlcoholFrontal CortexGlutamate TransporterGlutamate UptakeHippocampusPostnatal Stresshttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Postnatal stress alters stress responses for life, with serious consequences on the central nervous system (CNS), involving glutamatergic neurotransmission and development of alcohol voluntary intake. Several drugs of abuse, including alcohol and cocaine, alter glutamate transport (GluT). Here, we evaluated effects of chronic postnatal stress (CPS) on alcohol intake and brain glutamate uptake and transporters in male adolescent Wistar rats. For CPS from postnatal day (PD) 7, the pups were separated from their mothers and exposed to cold stress (4ºC) for 1h during 20 days; controls remained with their mothers. Then they were exposed to either voluntary ethanol (6%) or dextrose (1%) intake for 7 days (5-7 rats per group), then killed. CPS: 1) increased voluntary ethanol intake; 2) did not affect body weight gain or produce signs of toxicity with alcohol exposure; 3) increased glutamate uptake by hippocampal synaptosomes in vitro 4) reduced protein levels (Western measurements) in hippocampus and frontal cortex of glial glutamate transporter-1 (GLT-1) and excitatory amino-acid transporter-3 (EAAT-3) but increased glutamate aspartate transporter (GLAST) levels. We propose that CPS-induced decrements in GLT-1 and EAAT-3 expression levels are opposed by activation of a compensatory mechanism to prevent excitotoxicity. A greater role for GLAST in total glutamate uptake to put a stop to enlarged extracellular glutamate levels is inferred. The results also demonstrate that CPS strongly increased intake of ethanol, which had little impact on effects of CPS on brain glutamate uptake or transporters. However, the impact of early life adverse events on glutamatergic neurotransmission may underlie increased alcohol consumption in adulthood.Fil: Odeon, Maria Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Andreu, Marcela. Laboratorio Bioquímica Médica; ArgentinaFil: Yamauchi, Laura. Laboratorio Bioquímica Médica; ArgentinaFil: Grosman, Mauricio. Laboratorio Bioquímica Médica; ArgentinaFil: Acosta, Gabriela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaTaylor & Francis2015-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13597Odeon, Maria Mercedes; Andreu, Marcela; Yamauchi, Laura; Grosman, Mauricio; Acosta, Gabriela Beatriz; Chronic postnatal stress induces voluntary alcohol intake and modifies glutamate transporters in adolescent rats; Taylor & Francis; Stress; 2; 6-2015; 1-81025-38901607-8888enginfo:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.3109/10253890.2015.1041909info:eu-repo/semantics/altIdentifier/doi/10.3109/10253890.2015.1041909info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:37:08Zoai:ri.conicet.gov.ar:11336/13597instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:37:08.495CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Chronic postnatal stress induces voluntary alcohol intake and modifies glutamate transporters in adolescent rats
title Chronic postnatal stress induces voluntary alcohol intake and modifies glutamate transporters in adolescent rats
spellingShingle Chronic postnatal stress induces voluntary alcohol intake and modifies glutamate transporters in adolescent rats
Odeon, Maria Mercedes
Alcohol
Frontal Cortex
Glutamate Transporter
Glutamate Uptake
Hippocampus
Postnatal Stress
title_short Chronic postnatal stress induces voluntary alcohol intake and modifies glutamate transporters in adolescent rats
title_full Chronic postnatal stress induces voluntary alcohol intake and modifies glutamate transporters in adolescent rats
title_fullStr Chronic postnatal stress induces voluntary alcohol intake and modifies glutamate transporters in adolescent rats
title_full_unstemmed Chronic postnatal stress induces voluntary alcohol intake and modifies glutamate transporters in adolescent rats
title_sort Chronic postnatal stress induces voluntary alcohol intake and modifies glutamate transporters in adolescent rats
dc.creator.none.fl_str_mv Odeon, Maria Mercedes
Andreu, Marcela
Yamauchi, Laura
Grosman, Mauricio
Acosta, Gabriela Beatriz
author Odeon, Maria Mercedes
author_facet Odeon, Maria Mercedes
Andreu, Marcela
Yamauchi, Laura
Grosman, Mauricio
Acosta, Gabriela Beatriz
author_role author
author2 Andreu, Marcela
Yamauchi, Laura
Grosman, Mauricio
Acosta, Gabriela Beatriz
author2_role author
author
author
author
dc.subject.none.fl_str_mv Alcohol
Frontal Cortex
Glutamate Transporter
Glutamate Uptake
Hippocampus
Postnatal Stress
topic Alcohol
Frontal Cortex
Glutamate Transporter
Glutamate Uptake
Hippocampus
Postnatal Stress
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Postnatal stress alters stress responses for life, with serious consequences on the central nervous system (CNS), involving glutamatergic neurotransmission and development of alcohol voluntary intake. Several drugs of abuse, including alcohol and cocaine, alter glutamate transport (GluT). Here, we evaluated effects of chronic postnatal stress (CPS) on alcohol intake and brain glutamate uptake and transporters in male adolescent Wistar rats. For CPS from postnatal day (PD) 7, the pups were separated from their mothers and exposed to cold stress (4ºC) for 1h during 20 days; controls remained with their mothers. Then they were exposed to either voluntary ethanol (6%) or dextrose (1%) intake for 7 days (5-7 rats per group), then killed. CPS: 1) increased voluntary ethanol intake; 2) did not affect body weight gain or produce signs of toxicity with alcohol exposure; 3) increased glutamate uptake by hippocampal synaptosomes in vitro 4) reduced protein levels (Western measurements) in hippocampus and frontal cortex of glial glutamate transporter-1 (GLT-1) and excitatory amino-acid transporter-3 (EAAT-3) but increased glutamate aspartate transporter (GLAST) levels. We propose that CPS-induced decrements in GLT-1 and EAAT-3 expression levels are opposed by activation of a compensatory mechanism to prevent excitotoxicity. A greater role for GLAST in total glutamate uptake to put a stop to enlarged extracellular glutamate levels is inferred. The results also demonstrate that CPS strongly increased intake of ethanol, which had little impact on effects of CPS on brain glutamate uptake or transporters. However, the impact of early life adverse events on glutamatergic neurotransmission may underlie increased alcohol consumption in adulthood.
Fil: Odeon, Maria Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Andreu, Marcela. Laboratorio Bioquímica Médica; Argentina
Fil: Yamauchi, Laura. Laboratorio Bioquímica Médica; Argentina
Fil: Grosman, Mauricio. Laboratorio Bioquímica Médica; Argentina
Fil: Acosta, Gabriela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
description Postnatal stress alters stress responses for life, with serious consequences on the central nervous system (CNS), involving glutamatergic neurotransmission and development of alcohol voluntary intake. Several drugs of abuse, including alcohol and cocaine, alter glutamate transport (GluT). Here, we evaluated effects of chronic postnatal stress (CPS) on alcohol intake and brain glutamate uptake and transporters in male adolescent Wistar rats. For CPS from postnatal day (PD) 7, the pups were separated from their mothers and exposed to cold stress (4ºC) for 1h during 20 days; controls remained with their mothers. Then they were exposed to either voluntary ethanol (6%) or dextrose (1%) intake for 7 days (5-7 rats per group), then killed. CPS: 1) increased voluntary ethanol intake; 2) did not affect body weight gain or produce signs of toxicity with alcohol exposure; 3) increased glutamate uptake by hippocampal synaptosomes in vitro 4) reduced protein levels (Western measurements) in hippocampus and frontal cortex of glial glutamate transporter-1 (GLT-1) and excitatory amino-acid transporter-3 (EAAT-3) but increased glutamate aspartate transporter (GLAST) levels. We propose that CPS-induced decrements in GLT-1 and EAAT-3 expression levels are opposed by activation of a compensatory mechanism to prevent excitotoxicity. A greater role for GLAST in total glutamate uptake to put a stop to enlarged extracellular glutamate levels is inferred. The results also demonstrate that CPS strongly increased intake of ethanol, which had little impact on effects of CPS on brain glutamate uptake or transporters. However, the impact of early life adverse events on glutamatergic neurotransmission may underlie increased alcohol consumption in adulthood.
publishDate 2015
dc.date.none.fl_str_mv 2015-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13597
Odeon, Maria Mercedes; Andreu, Marcela; Yamauchi, Laura; Grosman, Mauricio; Acosta, Gabriela Beatriz; Chronic postnatal stress induces voluntary alcohol intake and modifies glutamate transporters in adolescent rats; Taylor & Francis; Stress; 2; 6-2015; 1-8
1025-3890
1607-8888
url http://hdl.handle.net/11336/13597
identifier_str_mv Odeon, Maria Mercedes; Andreu, Marcela; Yamauchi, Laura; Grosman, Mauricio; Acosta, Gabriela Beatriz; Chronic postnatal stress induces voluntary alcohol intake and modifies glutamate transporters in adolescent rats; Taylor & Francis; Stress; 2; 6-2015; 1-8
1025-3890
1607-8888
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.tandfonline.com/doi/full/10.3109/10253890.2015.1041909
info:eu-repo/semantics/altIdentifier/doi/10.3109/10253890.2015.1041909
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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