Consequences of postnatal stress: maternal separation in rats induces long-lasting changes on glutamate transporters

Autores
Odeon, Maria Mercedes; Salatino, Adrián Emanuel; Acosta, Gabriela Beatriz
Año de publicación
2013
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: There is increasing evidence that environmental factors, particularly stressful events experienced early in life, increase the risk of developing a psychiatric illness and/or a behavioural disorder. The aim of this study was to evaluate the effects of acute and chronic maternal separation (AMS and CMS) plus cold stress on the expression patterns of Glutamate Transporters (TGlus) in the developing and young adult Central Nervous System (CNS). As regulation of Glutamate (Glu) extracellular levels is of key importance, sodium-dependent Glu uptake using synaptosome-enriched fractions isolated from Frontal Cortex (FC) and Hippocampus (Hic) was also studied. Results: In animals under AMS stress found that Glu uptake decreases with respect to control groups. Meanwhile in CMS did not observe changes on Glu uptake in adult animals. These data would suggest the existence of an adaptive mechanism that could compensate the effect of AMS and CMS plus cold stress on glutamate uptake. Western blotting was performed in homogenates prepared from FC and Hic from both neonate and young adult rat brains. These blots exposed that homogenates include GLT-1 and EAAC-1 proteins and their levels varied in different areas of the rat brain and with the age of animals. Conclusions: During early postnatal life, exposure to various stressors lead to the development of various neurological, psychiatric, neurodegenerative and behavioral disorders, expressible in adult life in the case of chronic stress. These results demonstrate that adverse early life events have profound and persistent effects on brain function and may represent a risk factor for the development of psychopathology later life.
Fil: Odeon, Maria Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina
Fil: Salatino, Adrián Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina
Fil: Acosta, Gabriela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas. Cátedra de Fisiopatología; Argentina
Materia
acute
chronc
maternal separation
cold stress
glutamate transporters
uptake
Western blot
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/13691
Acceder
id CONICETDig_eb54fafbc953108fe2266612a7205b8f
oai_identifier_str oai:ri.conicet.gov.ar:11336/13691
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Consequences of postnatal stress: maternal separation in rats induces long-lasting changes on glutamate transportersOdeon, Maria MercedesSalatino, Adrián EmanuelAcosta, Gabriela Beatrizacutechroncmaternal separationcold stressglutamate transportersuptakeWestern blothttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Background: There is increasing evidence that environmental factors, particularly stressful events experienced early in life, increase the risk of developing a psychiatric illness and/or a behavioural disorder. The aim of this study was to evaluate the effects of acute and chronic maternal separation (AMS and CMS) plus cold stress on the expression patterns of Glutamate Transporters (TGlus) in the developing and young adult Central Nervous System (CNS). As regulation of Glutamate (Glu) extracellular levels is of key importance, sodium-dependent Glu uptake using synaptosome-enriched fractions isolated from Frontal Cortex (FC) and Hippocampus (Hic) was also studied. Results: In animals under AMS stress found that Glu uptake decreases with respect to control groups. Meanwhile in CMS did not observe changes on Glu uptake in adult animals. These data would suggest the existence of an adaptive mechanism that could compensate the effect of AMS and CMS plus cold stress on glutamate uptake. Western blotting was performed in homogenates prepared from FC and Hic from both neonate and young adult rat brains. These blots exposed that homogenates include GLT-1 and EAAC-1 proteins and their levels varied in different areas of the rat brain and with the age of animals. Conclusions: During early postnatal life, exposure to various stressors lead to the development of various neurological, psychiatric, neurodegenerative and behavioral disorders, expressible in adult life in the case of chronic stress. These results demonstrate that adverse early life events have profound and persistent effects on brain function and may represent a risk factor for the development of psychopathology later life.Fil: Odeon, Maria Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); ArgentinaFil: Salatino, Adrián Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); ArgentinaFil: Acosta, Gabriela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas. Cátedra de Fisiopatología; ArgentinaOMICS International2013-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/13691Odeon, Maria Mercedes; Salatino, Adrián Emanuel; Acosta, Gabriela Beatriz; Consequences of postnatal stress: maternal separation in rats induces long-lasting changes on glutamate transporters; OMICS International; Journal of Clinical & Experimental Pharmacology; 3; 121; 3-2013; 1-72161-1459enginfo:eu-repo/semantics/altIdentifier/url/https://www.omicsonline.org/consequences-of-postnatal-stress-maternal-separation-in-rats-induces-long-lasting-changes-on-glutamate-transporters-2161-1459.1000121.php?aid=12098info:eu-repo/semantics/altIdentifier/doi/10.4172/2161-1459.1000121info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:30:06Zoai:ri.conicet.gov.ar:11336/13691instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:30:06.984CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Consequences of postnatal stress: maternal separation in rats induces long-lasting changes on glutamate transporters
title Consequences of postnatal stress: maternal separation in rats induces long-lasting changes on glutamate transporters
spellingShingle Consequences of postnatal stress: maternal separation in rats induces long-lasting changes on glutamate transporters
Odeon, Maria Mercedes
acute
chronc
maternal separation
cold stress
glutamate transporters
uptake
Western blot
title_short Consequences of postnatal stress: maternal separation in rats induces long-lasting changes on glutamate transporters
title_full Consequences of postnatal stress: maternal separation in rats induces long-lasting changes on glutamate transporters
title_fullStr Consequences of postnatal stress: maternal separation in rats induces long-lasting changes on glutamate transporters
title_full_unstemmed Consequences of postnatal stress: maternal separation in rats induces long-lasting changes on glutamate transporters
title_sort Consequences of postnatal stress: maternal separation in rats induces long-lasting changes on glutamate transporters
dc.creator.none.fl_str_mv Odeon, Maria Mercedes
Salatino, Adrián Emanuel
Acosta, Gabriela Beatriz
author Odeon, Maria Mercedes
author_facet Odeon, Maria Mercedes
Salatino, Adrián Emanuel
Acosta, Gabriela Beatriz
author_role author
author2 Salatino, Adrián Emanuel
Acosta, Gabriela Beatriz
author2_role author
author
dc.subject.none.fl_str_mv acute
chronc
maternal separation
cold stress
glutamate transporters
uptake
Western blot
topic acute
chronc
maternal separation
cold stress
glutamate transporters
uptake
Western blot
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: There is increasing evidence that environmental factors, particularly stressful events experienced early in life, increase the risk of developing a psychiatric illness and/or a behavioural disorder. The aim of this study was to evaluate the effects of acute and chronic maternal separation (AMS and CMS) plus cold stress on the expression patterns of Glutamate Transporters (TGlus) in the developing and young adult Central Nervous System (CNS). As regulation of Glutamate (Glu) extracellular levels is of key importance, sodium-dependent Glu uptake using synaptosome-enriched fractions isolated from Frontal Cortex (FC) and Hippocampus (Hic) was also studied. Results: In animals under AMS stress found that Glu uptake decreases with respect to control groups. Meanwhile in CMS did not observe changes on Glu uptake in adult animals. These data would suggest the existence of an adaptive mechanism that could compensate the effect of AMS and CMS plus cold stress on glutamate uptake. Western blotting was performed in homogenates prepared from FC and Hic from both neonate and young adult rat brains. These blots exposed that homogenates include GLT-1 and EAAC-1 proteins and their levels varied in different areas of the rat brain and with the age of animals. Conclusions: During early postnatal life, exposure to various stressors lead to the development of various neurological, psychiatric, neurodegenerative and behavioral disorders, expressible in adult life in the case of chronic stress. These results demonstrate that adverse early life events have profound and persistent effects on brain function and may represent a risk factor for the development of psychopathology later life.
Fil: Odeon, Maria Mercedes. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina
Fil: Salatino, Adrián Emanuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina
Fil: Acosta, Gabriela Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Farmacológicas (i); Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Departamento de Ciencias Biológicas. Cátedra de Fisiopatología; Argentina
description Background: There is increasing evidence that environmental factors, particularly stressful events experienced early in life, increase the risk of developing a psychiatric illness and/or a behavioural disorder. The aim of this study was to evaluate the effects of acute and chronic maternal separation (AMS and CMS) plus cold stress on the expression patterns of Glutamate Transporters (TGlus) in the developing and young adult Central Nervous System (CNS). As regulation of Glutamate (Glu) extracellular levels is of key importance, sodium-dependent Glu uptake using synaptosome-enriched fractions isolated from Frontal Cortex (FC) and Hippocampus (Hic) was also studied. Results: In animals under AMS stress found that Glu uptake decreases with respect to control groups. Meanwhile in CMS did not observe changes on Glu uptake in adult animals. These data would suggest the existence of an adaptive mechanism that could compensate the effect of AMS and CMS plus cold stress on glutamate uptake. Western blotting was performed in homogenates prepared from FC and Hic from both neonate and young adult rat brains. These blots exposed that homogenates include GLT-1 and EAAC-1 proteins and their levels varied in different areas of the rat brain and with the age of animals. Conclusions: During early postnatal life, exposure to various stressors lead to the development of various neurological, psychiatric, neurodegenerative and behavioral disorders, expressible in adult life in the case of chronic stress. These results demonstrate that adverse early life events have profound and persistent effects on brain function and may represent a risk factor for the development of psychopathology later life.
publishDate 2013
dc.date.none.fl_str_mv 2013-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/13691
Odeon, Maria Mercedes; Salatino, Adrián Emanuel; Acosta, Gabriela Beatriz; Consequences of postnatal stress: maternal separation in rats induces long-lasting changes on glutamate transporters; OMICS International; Journal of Clinical & Experimental Pharmacology; 3; 121; 3-2013; 1-7
2161-1459
url http://hdl.handle.net/11336/13691
identifier_str_mv Odeon, Maria Mercedes; Salatino, Adrián Emanuel; Acosta, Gabriela Beatriz; Consequences of postnatal stress: maternal separation in rats induces long-lasting changes on glutamate transporters; OMICS International; Journal of Clinical & Experimental Pharmacology; 3; 121; 3-2013; 1-7
2161-1459
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.omicsonline.org/consequences-of-postnatal-stress-maternal-separation-in-rats-induces-long-lasting-changes-on-glutamate-transporters-2161-1459.1000121.php?aid=12098
info:eu-repo/semantics/altIdentifier/doi/10.4172/2161-1459.1000121
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv OMICS International
publisher.none.fl_str_mv OMICS International
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1846082775696801792
score 13.22299

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