Disentangling the Regulatory Response of Agrobacterium tumefaciens CHLDO to Glyphosate for Engineering Whole-Cell Phosphonate Biosensors

Autores
Masotti, Fiorella; Krink, Nicolas; Lencina, Nicolás Martín; Gottig Schor, Natalia; Ottado, Jorgelina; Nikel, Pablo I.
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Phosphonates (PHTs), organic compounds with astable C−P bond, are widely distributed in nature. Glyphosate(GP), a synthetic PHT, is extensively used in agriculture and hasbeen linked to various human health issues and environmentaldamage. Given the prevalence of GP, developing cost-effective, onsitemethods for GP detection is key for assessing pollution andreducing exposure risks. We adopted Agrobacterium tumefaciensCHLDO, a natural GP degrader, as a host and the source ofgenetic parts for constructing PHT biosensors. In this bacterialspecies, the phn gene cluster, encoding the C−P lyase pathway, isregulated by the PhnF transcriptional repressor. We selected thephnG promoter, which displays a dose-dependent response to GP,to build a set of whole-cell biosensors. Through stepwise genetic optimization of the transcriptional cascade, we created a whole-cellbiosensor capable of detecting GP in the 0.25−50 μM range in various samples, including soil and water.
Fil: Masotti, Fiorella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Krink, Nicolas. Technical University of Denmark; Dinamarca
Fil: Lencina, Nicolás Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Gottig Schor, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Ottado, Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Nikel, Pablo I.. Technical University of Denmark; Dinamarca
Materia
GLYPHOSATE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/263298
Acceder
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network_name_str CONICET Digital (CONICET)
spelling Disentangling the Regulatory Response of Agrobacterium tumefaciens CHLDO to Glyphosate for Engineering Whole-Cell Phosphonate BiosensorsMasotti, FiorellaKrink, NicolasLencina, Nicolás MartínGottig Schor, NataliaOttado, JorgelinaNikel, Pablo I.GLYPHOSATEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Phosphonates (PHTs), organic compounds with astable C−P bond, are widely distributed in nature. Glyphosate(GP), a synthetic PHT, is extensively used in agriculture and hasbeen linked to various human health issues and environmentaldamage. Given the prevalence of GP, developing cost-effective, onsitemethods for GP detection is key for assessing pollution andreducing exposure risks. We adopted Agrobacterium tumefaciensCHLDO, a natural GP degrader, as a host and the source ofgenetic parts for constructing PHT biosensors. In this bacterialspecies, the phn gene cluster, encoding the C−P lyase pathway, isregulated by the PhnF transcriptional repressor. We selected thephnG promoter, which displays a dose-dependent response to GP,to build a set of whole-cell biosensors. Through stepwise genetic optimization of the transcriptional cascade, we created a whole-cellbiosensor capable of detecting GP in the 0.25−50 μM range in various samples, including soil and water.Fil: Masotti, Fiorella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Krink, Nicolas. Technical University of Denmark; DinamarcaFil: Lencina, Nicolás Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Gottig Schor, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; ArgentinaFil: Ottado, Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Nikel, Pablo I.. Technical University of Denmark; DinamarcaAmerican Chemical Society2024-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/263298Masotti, Fiorella; Krink, Nicolas; Lencina, Nicolás Martín; Gottig Schor, Natalia; Ottado, Jorgelina; et al.; Disentangling the Regulatory Response of Agrobacterium tumefaciens CHLDO to Glyphosate for Engineering Whole-Cell Phosphonate Biosensors; American Chemical Society; ACS Synthetic Biology; 13; 10; 9-2024; 3430-34452161-5063CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acssynbio.4c00497info:eu-repo/semantics/altIdentifier/doi/10.1021/acssynbio.4c00497info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:21:06Zoai:ri.conicet.gov.ar:11336/263298instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:21:06.79CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Disentangling the Regulatory Response of Agrobacterium tumefaciens CHLDO to Glyphosate for Engineering Whole-Cell Phosphonate Biosensors
title Disentangling the Regulatory Response of Agrobacterium tumefaciens CHLDO to Glyphosate for Engineering Whole-Cell Phosphonate Biosensors
spellingShingle Disentangling the Regulatory Response of Agrobacterium tumefaciens CHLDO to Glyphosate for Engineering Whole-Cell Phosphonate Biosensors
Masotti, Fiorella
GLYPHOSATE
title_short Disentangling the Regulatory Response of Agrobacterium tumefaciens CHLDO to Glyphosate for Engineering Whole-Cell Phosphonate Biosensors
title_full Disentangling the Regulatory Response of Agrobacterium tumefaciens CHLDO to Glyphosate for Engineering Whole-Cell Phosphonate Biosensors
title_fullStr Disentangling the Regulatory Response of Agrobacterium tumefaciens CHLDO to Glyphosate for Engineering Whole-Cell Phosphonate Biosensors
title_full_unstemmed Disentangling the Regulatory Response of Agrobacterium tumefaciens CHLDO to Glyphosate for Engineering Whole-Cell Phosphonate Biosensors
title_sort Disentangling the Regulatory Response of Agrobacterium tumefaciens CHLDO to Glyphosate for Engineering Whole-Cell Phosphonate Biosensors
dc.creator.none.fl_str_mv Masotti, Fiorella
Krink, Nicolas
Lencina, Nicolás Martín
Gottig Schor, Natalia
Ottado, Jorgelina
Nikel, Pablo I.
author Masotti, Fiorella
author_facet Masotti, Fiorella
Krink, Nicolas
Lencina, Nicolás Martín
Gottig Schor, Natalia
Ottado, Jorgelina
Nikel, Pablo I.
author_role author
author2 Krink, Nicolas
Lencina, Nicolás Martín
Gottig Schor, Natalia
Ottado, Jorgelina
Nikel, Pablo I.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv GLYPHOSATE
topic GLYPHOSATE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Phosphonates (PHTs), organic compounds with astable C−P bond, are widely distributed in nature. Glyphosate(GP), a synthetic PHT, is extensively used in agriculture and hasbeen linked to various human health issues and environmentaldamage. Given the prevalence of GP, developing cost-effective, onsitemethods for GP detection is key for assessing pollution andreducing exposure risks. We adopted Agrobacterium tumefaciensCHLDO, a natural GP degrader, as a host and the source ofgenetic parts for constructing PHT biosensors. In this bacterialspecies, the phn gene cluster, encoding the C−P lyase pathway, isregulated by the PhnF transcriptional repressor. We selected thephnG promoter, which displays a dose-dependent response to GP,to build a set of whole-cell biosensors. Through stepwise genetic optimization of the transcriptional cascade, we created a whole-cellbiosensor capable of detecting GP in the 0.25−50 μM range in various samples, including soil and water.
Fil: Masotti, Fiorella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Krink, Nicolas. Technical University of Denmark; Dinamarca
Fil: Lencina, Nicolás Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Gottig Schor, Natalia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Procesos Biotecnológicos y Químicos Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Procesos Biotecnológicos y Químicos Rosario; Argentina
Fil: Ottado, Jorgelina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Nikel, Pablo I.. Technical University of Denmark; Dinamarca
description Phosphonates (PHTs), organic compounds with astable C−P bond, are widely distributed in nature. Glyphosate(GP), a synthetic PHT, is extensively used in agriculture and hasbeen linked to various human health issues and environmentaldamage. Given the prevalence of GP, developing cost-effective, onsitemethods for GP detection is key for assessing pollution andreducing exposure risks. We adopted Agrobacterium tumefaciensCHLDO, a natural GP degrader, as a host and the source ofgenetic parts for constructing PHT biosensors. In this bacterialspecies, the phn gene cluster, encoding the C−P lyase pathway, isregulated by the PhnF transcriptional repressor. We selected thephnG promoter, which displays a dose-dependent response to GP,to build a set of whole-cell biosensors. Through stepwise genetic optimization of the transcriptional cascade, we created a whole-cellbiosensor capable of detecting GP in the 0.25−50 μM range in various samples, including soil and water.
publishDate 2024
dc.date.none.fl_str_mv 2024-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/263298
Masotti, Fiorella; Krink, Nicolas; Lencina, Nicolás Martín; Gottig Schor, Natalia; Ottado, Jorgelina; et al.; Disentangling the Regulatory Response of Agrobacterium tumefaciens CHLDO to Glyphosate for Engineering Whole-Cell Phosphonate Biosensors; American Chemical Society; ACS Synthetic Biology; 13; 10; 9-2024; 3430-3445
2161-5063
CONICET Digital
CONICET
url http://hdl.handle.net/11336/263298
identifier_str_mv Masotti, Fiorella; Krink, Nicolas; Lencina, Nicolás Martín; Gottig Schor, Natalia; Ottado, Jorgelina; et al.; Disentangling the Regulatory Response of Agrobacterium tumefaciens CHLDO to Glyphosate for Engineering Whole-Cell Phosphonate Biosensors; American Chemical Society; ACS Synthetic Biology; 13; 10; 9-2024; 3430-3445
2161-5063
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://pubs.acs.org/doi/10.1021/acssynbio.4c00497
info:eu-repo/semantics/altIdentifier/doi/10.1021/acssynbio.4c00497
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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