Efficient Agrobacterium tumefaciens-mediated transformation system of Diaporthe caulivora

Autores
Montoya, Marina Rosa; Massa, Gabriela Alejandra; Colabelli, Mabel Noemí; Ridao, Azucena
Año de publicación
2021
Idioma
español castellano
Tipo de recurso
artículo
Estado
versión publicada
Descripción
This is the first report describing the genetic transformation of Diaporthe caulivora, the soybean stem canker fungus. A simple and 100% efficient protocol of Agrobacterium tumefaciens-mediated transformation used mycelium as starting material and the hygromycin B resistance and green fluorescent protein (GFP) as a selection and reporter agents, respectively. All transgenic isolates were mitotically stable in two independent experiments and polymerase chain reaction with hygromycin B resistance primers confirmed successful T-DNA integration into the fungal genome. Plant-fungus interaction studies, including pathogenicity, latency, and endophytism, as well as further studies of random and targeted mutagenesis will be possible with GFP-expressing isolates of D. caulivora and other species in the Diaporthe / Phomopsis complex.
EEA Balcarce
Fil: Montoya, Marina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.
Fil: Massa. Gabriela Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.
Fil: Massa. Gabriela Alejandra. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Colabelli, Mabel Noemí. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Ridao, Azucena del Carmen. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fuente
Journal of Microbiological Methods 184 : 106197 (May 2021)
Materia
Soja
Tallos
Diaporthe
Agrobacterium Tumefaciens
Infecciones Latentes
Soybeans
Stems
Latent Infection
Diaporthe caulivora
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/10234
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spelling Efficient Agrobacterium tumefaciens-mediated transformation system of Diaporthe caulivoraMontoya, Marina RosaMassa, Gabriela AlejandraColabelli, Mabel NoemíRidao, AzucenaSojaTallosDiaportheAgrobacterium TumefaciensInfecciones LatentesSoybeansStemsLatent InfectionDiaporthe caulivoraThis is the first report describing the genetic transformation of Diaporthe caulivora, the soybean stem canker fungus. A simple and 100% efficient protocol of Agrobacterium tumefaciens-mediated transformation used mycelium as starting material and the hygromycin B resistance and green fluorescent protein (GFP) as a selection and reporter agents, respectively. All transgenic isolates were mitotically stable in two independent experiments and polymerase chain reaction with hygromycin B resistance primers confirmed successful T-DNA integration into the fungal genome. Plant-fungus interaction studies, including pathogenicity, latency, and endophytism, as well as further studies of random and targeted mutagenesis will be possible with GFP-expressing isolates of D. caulivora and other species in the Diaporthe / Phomopsis complex.EEA BalcarceFil: Montoya, Marina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.Fil: Massa. Gabriela Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.Fil: Massa. Gabriela Alejandra. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Colabelli, Mabel Noemí. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Fil: Ridao, Azucena del Carmen. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.Elsevier2021-09-13T13:16:34Z2021-09-13T13:16:34Z2021-03-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10234https://www.sciencedirect.com/science/article/pii/S01677012210006580167-7012https://doi.org/10.1016/j.mimet.2021.106197Journal of Microbiological Methods 184 : 106197 (May 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaspainfo:eu-repograntAgreement/INTA/PNPV-1135024/AR./Defensa en plantas contra fitopatógenos.info:eu-repograntAgreement/INTA/PNCYO-1127034/AR./Evaluación y desarrollo de sistemas de manejo integrado de las plagas en cultivos de cereales y oleaginosas.info:eu-repo/semantics/restrictedAccess2025-09-04T09:49:05Zoai:localhost:20.500.12123/10234instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:49:06.138INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Efficient Agrobacterium tumefaciens-mediated transformation system of Diaporthe caulivora
title Efficient Agrobacterium tumefaciens-mediated transformation system of Diaporthe caulivora
spellingShingle Efficient Agrobacterium tumefaciens-mediated transformation system of Diaporthe caulivora
Montoya, Marina Rosa
Soja
Tallos
Diaporthe
Agrobacterium Tumefaciens
Infecciones Latentes
Soybeans
Stems
Latent Infection
Diaporthe caulivora
title_short Efficient Agrobacterium tumefaciens-mediated transformation system of Diaporthe caulivora
title_full Efficient Agrobacterium tumefaciens-mediated transformation system of Diaporthe caulivora
title_fullStr Efficient Agrobacterium tumefaciens-mediated transformation system of Diaporthe caulivora
title_full_unstemmed Efficient Agrobacterium tumefaciens-mediated transformation system of Diaporthe caulivora
title_sort Efficient Agrobacterium tumefaciens-mediated transformation system of Diaporthe caulivora
dc.creator.none.fl_str_mv Montoya, Marina Rosa
Massa, Gabriela Alejandra
Colabelli, Mabel Noemí
Ridao, Azucena
author Montoya, Marina Rosa
author_facet Montoya, Marina Rosa
Massa, Gabriela Alejandra
Colabelli, Mabel Noemí
Ridao, Azucena
author_role author
author2 Massa, Gabriela Alejandra
Colabelli, Mabel Noemí
Ridao, Azucena
author2_role author
author
author
dc.subject.none.fl_str_mv Soja
Tallos
Diaporthe
Agrobacterium Tumefaciens
Infecciones Latentes
Soybeans
Stems
Latent Infection
Diaporthe caulivora
topic Soja
Tallos
Diaporthe
Agrobacterium Tumefaciens
Infecciones Latentes
Soybeans
Stems
Latent Infection
Diaporthe caulivora
dc.description.none.fl_txt_mv This is the first report describing the genetic transformation of Diaporthe caulivora, the soybean stem canker fungus. A simple and 100% efficient protocol of Agrobacterium tumefaciens-mediated transformation used mycelium as starting material and the hygromycin B resistance and green fluorescent protein (GFP) as a selection and reporter agents, respectively. All transgenic isolates were mitotically stable in two independent experiments and polymerase chain reaction with hygromycin B resistance primers confirmed successful T-DNA integration into the fungal genome. Plant-fungus interaction studies, including pathogenicity, latency, and endophytism, as well as further studies of random and targeted mutagenesis will be possible with GFP-expressing isolates of D. caulivora and other species in the Diaporthe / Phomopsis complex.
EEA Balcarce
Fil: Montoya, Marina Rosa. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.
Fil: Massa. Gabriela Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible; Argentina.
Fil: Massa. Gabriela Alejandra. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Colabelli, Mabel Noemí. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
Fil: Ridao, Azucena del Carmen. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina.
description This is the first report describing the genetic transformation of Diaporthe caulivora, the soybean stem canker fungus. A simple and 100% efficient protocol of Agrobacterium tumefaciens-mediated transformation used mycelium as starting material and the hygromycin B resistance and green fluorescent protein (GFP) as a selection and reporter agents, respectively. All transgenic isolates were mitotically stable in two independent experiments and polymerase chain reaction with hygromycin B resistance primers confirmed successful T-DNA integration into the fungal genome. Plant-fungus interaction studies, including pathogenicity, latency, and endophytism, as well as further studies of random and targeted mutagenesis will be possible with GFP-expressing isolates of D. caulivora and other species in the Diaporthe / Phomopsis complex.
publishDate 2021
dc.date.none.fl_str_mv 2021-09-13T13:16:34Z
2021-09-13T13:16:34Z
2021-03-10
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/10234
https://www.sciencedirect.com/science/article/pii/S0167701221000658
0167-7012
https://doi.org/10.1016/j.mimet.2021.106197
url http://hdl.handle.net/20.500.12123/10234
https://www.sciencedirect.com/science/article/pii/S0167701221000658
https://doi.org/10.1016/j.mimet.2021.106197
identifier_str_mv 0167-7012
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/PNPV-1135024/AR./Defensa en plantas contra fitopatógenos.
info:eu-repograntAgreement/INTA/PNCYO-1127034/AR./Evaluación y desarrollo de sistemas de manejo integrado de las plagas en cultivos de cereales y oleaginosas.
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Journal of Microbiological Methods 184 : 106197 (May 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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