Mini-FLOTAC, Kato-Katz and McMaster: three methods, one goal; highlights from north Argentina

Autores
Barda, Beatrice; Cajal, Pamela; Villagran, Eliana; Cimino, Ruben; Juarez, Marisa; Krolewiecki, Alejandro Javier; Rinaldi, Laura; Cringoli, Giuseppe; Burioni, Roberto; Albonico, Marco
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Background: Copro-parasitological diagnosis is still a challenge in management of helminth infections at individualand community levels in resource-limited settings.The aim of our study was to compare the performance of three quantitative techniques: Kato-Katz, McMaster andMini-FLOTAC methids. The study was carried out in Oran, Northern Argentina.Methods: 200 schoolchildren were enrolled to provide a single stool sample, which was tested for helminthinfections with Kato-Katz, McMaster and Mini-FLOTAC methods. The Mini-FLOTAC was performed with two flotationsolutions (FS2 saturated saline and FS7 zinc sulphate). Preparation and reading time for each of the three methodswas calculated both when processing single and multiple samples.Results: Out of 193 schoolchildren examined, 40% were positive for any helminth infection by any method; themost prevalent was Hymenolepis nana (23%) followed by Ascaris lumbricoides (17%) and a third group of lessprevalent helminths: Enterobius vermicularis, Trichuris trichiura and hookworms (11% all together). Mini-FLOTAC FS2was more sensitive than FS7 for H. nana (93% vs 78%) and for other helminths (85% vs 80%), whereas FS7 was moresensitive for A. lumbricoides (87% vs 61%). Kato-Katz method was more sensitive than McMaster method for A.lumbricoides (84% vs 48%) and for other helminths (48% vs 43%) except for H. nana (49% vs 61%). As for eggcounts, Mini-FLOTAC FS2 reported 904 eggs per gram of faeces (EPG) for H. nana (vs 457 with McMaster and 111with Kato-Katz) and 1177 EPG for A. lumbricoides (vs 1315 with Kato-Katz and 995 with McMaster); FS2 detected thehighest EPG for both H.nana and A.lumbricoides (904 vs 568 and 1177 vs 643 respectively), the differences werenot statistically significant. The technique feasibility was calculated: Kato-Katz mean time was 48 minutes/sample,Mini-FLOTAC 13 minutes/sample and McMaster 7 minutes/sample. However, especially for Kato-Katz and Mini-FLOTAC,the mean time (min/sample) decreased significantly when processing multiple samples.Conclusions: Mini-FLOTAC is a promising technique for helminth diagnosis, it is more sensitive than Kato-Katz andMcMaster for H. nana and as sensitive as Kato-Katz and more sensitive than McMaster for A. lumbricoides identification.Egg counts differences although relevant, did not reach statistical significance.
Fil: Barda, Beatrice. San Raffaele Hospital; Italia
Fil: Cajal, Pamela. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Villagran, Eliana. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Cimino, Ruben. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Juarez, Marisa. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Krolewiecki, Alejandro Javier. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina
Fil: Rinaldi, Laura. Universidad de Napoles Federico III; Italia
Fil: Cringoli, Giuseppe. Universidad de Napoles Federico III; Italia
Fil: Burioni, Roberto. San Raffaele Hospital; Italia
Fil: Albonico, Marco. Ivo de Carneri Foundation; Italia
Materia
MINI FLOTAC
GEOHELMINTOS
ASCARIS
DIAGNOSTICOS
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/7234
Acceder
id CONICETDig_a24a41db3912abad376407ad89e32f0c
oai_identifier_str oai:ri.conicet.gov.ar:11336/7234
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Mini-FLOTAC, Kato-Katz and McMaster: three methods, one goal; highlights from north ArgentinaBarda, BeatriceCajal, PamelaVillagran, ElianaCimino, RubenJuarez, MarisaKrolewiecki, Alejandro JavierRinaldi, LauraCringoli, GiuseppeBurioni, RobertoAlbonico, MarcoMINI FLOTACGEOHELMINTOSASCARISDIAGNOSTICOShttps://purl.org/becyt/ford/3.3https://purl.org/becyt/ford/3Background: Copro-parasitological diagnosis is still a challenge in management of helminth infections at individualand community levels in resource-limited settings.The aim of our study was to compare the performance of three quantitative techniques: Kato-Katz, McMaster andMini-FLOTAC methids. The study was carried out in Oran, Northern Argentina.Methods: 200 schoolchildren were enrolled to provide a single stool sample, which was tested for helminthinfections with Kato-Katz, McMaster and Mini-FLOTAC methods. The Mini-FLOTAC was performed with two flotationsolutions (FS2 saturated saline and FS7 zinc sulphate). Preparation and reading time for each of the three methodswas calculated both when processing single and multiple samples.Results: Out of 193 schoolchildren examined, 40% were positive for any helminth infection by any method; themost prevalent was Hymenolepis nana (23%) followed by Ascaris lumbricoides (17%) and a third group of lessprevalent helminths: Enterobius vermicularis, Trichuris trichiura and hookworms (11% all together). Mini-FLOTAC FS2was more sensitive than FS7 for H. nana (93% vs 78%) and for other helminths (85% vs 80%), whereas FS7 was moresensitive for A. lumbricoides (87% vs 61%). Kato-Katz method was more sensitive than McMaster method for A.lumbricoides (84% vs 48%) and for other helminths (48% vs 43%) except for H. nana (49% vs 61%). As for eggcounts, Mini-FLOTAC FS2 reported 904 eggs per gram of faeces (EPG) for H. nana (vs 457 with McMaster and 111with Kato-Katz) and 1177 EPG for A. lumbricoides (vs 1315 with Kato-Katz and 995 with McMaster); FS2 detected thehighest EPG for both H.nana and A.lumbricoides (904 vs 568 and 1177 vs 643 respectively), the differences werenot statistically significant. The technique feasibility was calculated: Kato-Katz mean time was 48 minutes/sample,Mini-FLOTAC 13 minutes/sample and McMaster 7 minutes/sample. However, especially for Kato-Katz and Mini-FLOTAC,the mean time (min/sample) decreased significantly when processing multiple samples.Conclusions: Mini-FLOTAC is a promising technique for helminth diagnosis, it is more sensitive than Kato-Katz andMcMaster for H. nana and as sensitive as Kato-Katz and more sensitive than McMaster for A. lumbricoides identification.Egg counts differences although relevant, did not reach statistical significance.Fil: Barda, Beatrice. San Raffaele Hospital; ItaliaFil: Cajal, Pamela. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; ArgentinaFil: Villagran, Eliana. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; ArgentinaFil: Cimino, Ruben. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; ArgentinaFil: Juarez, Marisa. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; ArgentinaFil: Krolewiecki, Alejandro Javier. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; ArgentinaFil: Rinaldi, Laura. Universidad de Napoles Federico III; ItaliaFil: Cringoli, Giuseppe. Universidad de Napoles Federico III; ItaliaFil: Burioni, Roberto. San Raffaele Hospital; ItaliaFil: Albonico, Marco. Ivo de Carneri Foundation; ItaliaBiomed Central2014-06info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/7234Barda, Beatrice; Cajal, Pamela; Villagran, Eliana; Cimino, Ruben; Juarez, Marisa; et al.; Mini-FLOTAC, Kato-Katz and McMaster: three methods, one goal; highlights from north Argentina; Biomed Central; Parasites And Vectors; 7; 271; 6-2014; 1-71756-3305enginfo:eu-repo/semantics/altIdentifier/doi/10.1186/1756-3305-7-271info:eu-repo/semantics/altIdentifier/doi/info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24929554/info:eu-repo/semantics/altIdentifier/pmid/24929554info:eu-repo/semantics/altIdentifier/url/https://parasitesandvectors.biomedcentral.com/articles/10.1186/1756-3305-7-271info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:53Zoai:ri.conicet.gov.ar:11336/7234instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:53.538CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Mini-FLOTAC, Kato-Katz and McMaster: three methods, one goal; highlights from north Argentina
title Mini-FLOTAC, Kato-Katz and McMaster: three methods, one goal; highlights from north Argentina
spellingShingle Mini-FLOTAC, Kato-Katz and McMaster: three methods, one goal; highlights from north Argentina
Barda, Beatrice
MINI FLOTAC
GEOHELMINTOS
ASCARIS
DIAGNOSTICOS
title_short Mini-FLOTAC, Kato-Katz and McMaster: three methods, one goal; highlights from north Argentina
title_full Mini-FLOTAC, Kato-Katz and McMaster: three methods, one goal; highlights from north Argentina
title_fullStr Mini-FLOTAC, Kato-Katz and McMaster: three methods, one goal; highlights from north Argentina
title_full_unstemmed Mini-FLOTAC, Kato-Katz and McMaster: three methods, one goal; highlights from north Argentina
title_sort Mini-FLOTAC, Kato-Katz and McMaster: three methods, one goal; highlights from north Argentina
dc.creator.none.fl_str_mv Barda, Beatrice
Cajal, Pamela
Villagran, Eliana
Cimino, Ruben
Juarez, Marisa
Krolewiecki, Alejandro Javier
Rinaldi, Laura
Cringoli, Giuseppe
Burioni, Roberto
Albonico, Marco
author Barda, Beatrice
author_facet Barda, Beatrice
Cajal, Pamela
Villagran, Eliana
Cimino, Ruben
Juarez, Marisa
Krolewiecki, Alejandro Javier
Rinaldi, Laura
Cringoli, Giuseppe
Burioni, Roberto
Albonico, Marco
author_role author
author2 Cajal, Pamela
Villagran, Eliana
Cimino, Ruben
Juarez, Marisa
Krolewiecki, Alejandro Javier
Rinaldi, Laura
Cringoli, Giuseppe
Burioni, Roberto
Albonico, Marco
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv MINI FLOTAC
GEOHELMINTOS
ASCARIS
DIAGNOSTICOS
topic MINI FLOTAC
GEOHELMINTOS
ASCARIS
DIAGNOSTICOS
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.3
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Background: Copro-parasitological diagnosis is still a challenge in management of helminth infections at individualand community levels in resource-limited settings.The aim of our study was to compare the performance of three quantitative techniques: Kato-Katz, McMaster andMini-FLOTAC methids. The study was carried out in Oran, Northern Argentina.Methods: 200 schoolchildren were enrolled to provide a single stool sample, which was tested for helminthinfections with Kato-Katz, McMaster and Mini-FLOTAC methods. The Mini-FLOTAC was performed with two flotationsolutions (FS2 saturated saline and FS7 zinc sulphate). Preparation and reading time for each of the three methodswas calculated both when processing single and multiple samples.Results: Out of 193 schoolchildren examined, 40% were positive for any helminth infection by any method; themost prevalent was Hymenolepis nana (23%) followed by Ascaris lumbricoides (17%) and a third group of lessprevalent helminths: Enterobius vermicularis, Trichuris trichiura and hookworms (11% all together). Mini-FLOTAC FS2was more sensitive than FS7 for H. nana (93% vs 78%) and for other helminths (85% vs 80%), whereas FS7 was moresensitive for A. lumbricoides (87% vs 61%). Kato-Katz method was more sensitive than McMaster method for A.lumbricoides (84% vs 48%) and for other helminths (48% vs 43%) except for H. nana (49% vs 61%). As for eggcounts, Mini-FLOTAC FS2 reported 904 eggs per gram of faeces (EPG) for H. nana (vs 457 with McMaster and 111with Kato-Katz) and 1177 EPG for A. lumbricoides (vs 1315 with Kato-Katz and 995 with McMaster); FS2 detected thehighest EPG for both H.nana and A.lumbricoides (904 vs 568 and 1177 vs 643 respectively), the differences werenot statistically significant. The technique feasibility was calculated: Kato-Katz mean time was 48 minutes/sample,Mini-FLOTAC 13 minutes/sample and McMaster 7 minutes/sample. However, especially for Kato-Katz and Mini-FLOTAC,the mean time (min/sample) decreased significantly when processing multiple samples.Conclusions: Mini-FLOTAC is a promising technique for helminth diagnosis, it is more sensitive than Kato-Katz andMcMaster for H. nana and as sensitive as Kato-Katz and more sensitive than McMaster for A. lumbricoides identification.Egg counts differences although relevant, did not reach statistical significance.
Fil: Barda, Beatrice. San Raffaele Hospital; Italia
Fil: Cajal, Pamela. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Villagran, Eliana. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Cimino, Ruben. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Juarez, Marisa. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; Argentina
Fil: Krolewiecki, Alejandro Javier. Universidad Nacional de Salta. Sede Regional Oran. Instituto de Investigación de Enfermedades Tropicales; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Salta. Instituto de Patología Experimental; Argentina
Fil: Rinaldi, Laura. Universidad de Napoles Federico III; Italia
Fil: Cringoli, Giuseppe. Universidad de Napoles Federico III; Italia
Fil: Burioni, Roberto. San Raffaele Hospital; Italia
Fil: Albonico, Marco. Ivo de Carneri Foundation; Italia
description Background: Copro-parasitological diagnosis is still a challenge in management of helminth infections at individualand community levels in resource-limited settings.The aim of our study was to compare the performance of three quantitative techniques: Kato-Katz, McMaster andMini-FLOTAC methids. The study was carried out in Oran, Northern Argentina.Methods: 200 schoolchildren were enrolled to provide a single stool sample, which was tested for helminthinfections with Kato-Katz, McMaster and Mini-FLOTAC methods. The Mini-FLOTAC was performed with two flotationsolutions (FS2 saturated saline and FS7 zinc sulphate). Preparation and reading time for each of the three methodswas calculated both when processing single and multiple samples.Results: Out of 193 schoolchildren examined, 40% were positive for any helminth infection by any method; themost prevalent was Hymenolepis nana (23%) followed by Ascaris lumbricoides (17%) and a third group of lessprevalent helminths: Enterobius vermicularis, Trichuris trichiura and hookworms (11% all together). Mini-FLOTAC FS2was more sensitive than FS7 for H. nana (93% vs 78%) and for other helminths (85% vs 80%), whereas FS7 was moresensitive for A. lumbricoides (87% vs 61%). Kato-Katz method was more sensitive than McMaster method for A.lumbricoides (84% vs 48%) and for other helminths (48% vs 43%) except for H. nana (49% vs 61%). As for eggcounts, Mini-FLOTAC FS2 reported 904 eggs per gram of faeces (EPG) for H. nana (vs 457 with McMaster and 111with Kato-Katz) and 1177 EPG for A. lumbricoides (vs 1315 with Kato-Katz and 995 with McMaster); FS2 detected thehighest EPG for both H.nana and A.lumbricoides (904 vs 568 and 1177 vs 643 respectively), the differences werenot statistically significant. The technique feasibility was calculated: Kato-Katz mean time was 48 minutes/sample,Mini-FLOTAC 13 minutes/sample and McMaster 7 minutes/sample. However, especially for Kato-Katz and Mini-FLOTAC,the mean time (min/sample) decreased significantly when processing multiple samples.Conclusions: Mini-FLOTAC is a promising technique for helminth diagnosis, it is more sensitive than Kato-Katz andMcMaster for H. nana and as sensitive as Kato-Katz and more sensitive than McMaster for A. lumbricoides identification.Egg counts differences although relevant, did not reach statistical significance.
publishDate 2014
dc.date.none.fl_str_mv 2014-06
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/7234
Barda, Beatrice; Cajal, Pamela; Villagran, Eliana; Cimino, Ruben; Juarez, Marisa; et al.; Mini-FLOTAC, Kato-Katz and McMaster: three methods, one goal; highlights from north Argentina; Biomed Central; Parasites And Vectors; 7; 271; 6-2014; 1-7
1756-3305
url http://hdl.handle.net/11336/7234
identifier_str_mv Barda, Beatrice; Cajal, Pamela; Villagran, Eliana; Cimino, Ruben; Juarez, Marisa; et al.; Mini-FLOTAC, Kato-Katz and McMaster: three methods, one goal; highlights from north Argentina; Biomed Central; Parasites And Vectors; 7; 271; 6-2014; 1-7
1756-3305
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1186/1756-3305-7-271
info:eu-repo/semantics/altIdentifier/doi/
info:eu-repo/semantics/altIdentifier/url/http://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24929554/
info:eu-repo/semantics/altIdentifier/pmid/24929554
info:eu-repo/semantics/altIdentifier/url/https://parasitesandvectors.biomedcentral.com/articles/10.1186/1756-3305-7-271
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Biomed Central
publisher.none.fl_str_mv Biomed Central
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842268695545511936
score 13.13397

Publicaciones similares