Identifying thresholds to classify moderate-to-heavy soil-transmitted helminth intensity infections for FECPAKG2, McMaster, Mini-FLOTAC and qPCR

• Autores: Levecke, Bruno; Cools, Piet; Albonico, Marco; Ame, Shaali; Angebault, Cécile; Ayana, Mio; Behnke, Jerzy M.; Bethony, Jeffrey M.; Cringoli, Giuseppe; Dana, Daniel; Guillard, Bertrand; Viet Hoa, Nguyen Thi; Kang, Gagandeep; Kattula, Deepthi; Keiser, Jennifer; Kotze, Andrew C.; Matoso, Leonardo F.; Maurelli, Maria P.; McCarthy, James S.; Mekonnen, Zeleke; Mirams, Greg; Montresor, Antonio; Corrêa Oliveira, Rodrigo; Periago, Maria Victoria; Pinto, Simone A.; Rinaldi, Laura; Sayasone, Somphou; Sumo, Laurentine; Tchuem Tchuenté, Louis Albert; Cam Thach, Dang Thi; Thomas, Eurion; Zeynudin, Ahmed; Verweij, Jaco J.; Vlaminck, Johnny; Vercruysse, Jozef
• Año de publicación: 2020
• Idioma: inglés
• Tipo de recurso: artículo
• Materia: SOIL-TRANSMITTED HELMINTHS; INTENSITY; MCMASTER; QPCR
• Institución: Consejo Nacional de Investigaciones Científicas y Técnicas

World Health Organization (WHO) has defined moderate-to-heavy intensity (M&HI) infections with soil-transmitted helminths (Ascaris lumbricoides, Trichuris trichiura and the two hookworms, Ancylostoma duodenale and Necator americanus) based on specific values of eggs per gram of stool, as measured by the Kato-Katz method. There are a variety of novel microscopy and DNA-based methods but it remains unclear whether applying current WHO thresholds on to these methods allows for a reliable classification of M&HI infections. We evaluated both WHO and method-specific thresholds for classifying the M&HI infections for novel microscopic (FECPAKG2, McMaster and Mini-FLOTAC) and DNA-based (qPCR) diagnostic methods. For this, we determined method-specific thresholds that best classified M&HI infections (defined by Kato-Katz and WHO thresholds; reference method) in two multi-country drug efficacy studies. Subsequently, we verified whether applying these method-specific thresholds improved the agreement in classifying M&HI infections compared to the reference method. When we applied the WHO thresholds, the new microscopic methods mainly misclassified M&HI as low intensity, and to a lesser extent low intensity infection as M&HI. For FECPAKG2, applying the method-specific thresholds significantly improved the agreement for Ascaris (moderate → substantial), Trichuris and hookworms (fair → moderate). For Mini-FLOTAC, a significantly improved agreement was observed for hookworms only (fair → moderate). For the other STHs, the agreement was almost perfect and remained unchanged. For McMaster, the method-specific thresholds revealed a fair to a substantial agreement but did not significantly improve the agreement. For qPCR, the method-specific thresholds based on genome equivalents per ml of DNA moderately agreed with the reference method for hookworms and Trichuris infections. For Ascaris, there was a substantial agreement. We defined method-specific thresholds that improved the classification of M&HI infections. Validation studies are required before they can be recommended for general use in assessing M&HI infections in programmatic settings.
Fil: Levecke, Bruno. University Of Ghent. Faculty Of Sciences; Bélgica
Fil: Cools, Piet. University of Ghent; Bélgica
Fil: Albonico, Marco. University Of Turin; Italia
Fil: Ame, Shaali. Public Health Laboratory-ivo de Carneri; Tanzania
Fil: Angebault, Cécile. Institut Pasteur; Camboya
Fil: Ayana, Mio. Jimma University; Etiopía
Fil: Behnke, Jerzy M.. University of Nottingham; Estados Unidos
Fil: Bethony, Jeffrey M.. The George Washington University; Estados Unidos
Fil: Cringoli, Giuseppe. Università degli Studi di Napoli Federico II; Italia
Fil: Dana, Daniel. Jimma University; Etiopía
Fil: Guillard, Bertrand. Institut Pasteur; Camboya
Fil: Viet Hoa, Nguyen Thi. National Institute Of Malariology, Parasitology And Ent; Vietnam
Fil: Kang, Gagandeep. Christian Medical College; India
Fil: Kattula, Deepthi. Christian Medical College; India
Fil: Keiser, Jennifer. University of Basel; Suiza
Fil: Kotze, Andrew C.. Commonwealth Scientific and Industrial Research Organization; Australia
Fil: Matoso, Leonardo F.. Centro de Pesquisa René Rachou; Brasil
Fil: Maurelli, Maria P.. Università degli Studi di Napoli Federico II; Italia
Fil: McCarthy, James S.. University of Queensland; Australia
Fil: Mekonnen, Zeleke. Jimma University; Etiopía
Fil: Mirams, Greg. Techion Group Ltd; Nueva Zelanda
Fil: Montresor, Antonio. World Health Organization; Suiza
Fil: Corrêa Oliveira, Rodrigo. Centro de Pesquisa René Rachou; Brasil
Fil: Periago, Maria Victoria. Fundación Mundo Sano; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pinto, Simone A.. Centro de Pesquisa René Rachou; Brasil
Fil: Rinaldi, Laura. Università degli Studi di Napoli Federico II; Italia
Fil: Sayasone, Somphou. Ministry of Health; Laos
Fil: Sumo, Laurentine. University Of Yaounde I; Camerún
Fil: Tchuem Tchuenté, Louis Albert. University Of Yaounde I; Camerún
Fil: Cam Thach, Dang Thi. National Institute Of Malariology, Parasitology And Ent; Vietnam
Fil: Thomas, Eurion. Techion Group Ltd; Reino Unido
Fil: Zeynudin, Ahmed. Jimma University; Etiopía
Fil: Verweij, Jaco J.. Elisabeth-tweesteden Hospital; Países Bajos
Fil: Vlaminck, Johnny. University of Ghent; Bélgica
Fil: Vercruysse, Jozef. University of Ghent; Bélgica