Culture Medium Factorial Design Optimization for Fibrinolytic Enzymes Production by Bionectria sp.

Autores
Arnau, Victor Gonzalo; Rovati, Jose Ignacio; Figueroa, L. I. C.; Fariña, Julia Ines
Año de publicación
2010
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Thrombotic diseases can be clinically treated with fibrinolytic enzymes and many attempts have been made at laboratory level to increase fibrinolytic enzymes production from microbial sources and to reduce the process cost, including culture medium design, optimization of environmental conditions, and over expression with genetically modified strains. In this contribution we present the optimization of culture medium composition and incubation temperature for fibrinolytic enzyme production by Bionectria sp., a selected fungal strain from Las Yungas (Tucumán). Optimization was carried out at Erlenmeyer scale (100-mL working volume) via factorial design methodology. All trials included a common mineral base (%, w/v: NaCl 0.2, KH2PO4 0.05, MgSO4·7H2O 0.05). According to four factorial designs it could be demonstrated the convenience of using soy peptone as N-source, glucose as C-source, and the possibility to eliminate starch, meat peptone and meat extract from original medium composition, whilst 25°C was selected as the optimal incubation temperature. Results showed that culture medium could be successfully optimized by factorial design, achieving a reduction in the production process costs by means of a decrease in culture medium components, the improvement in culture broth rheology, mycelial morphology and mass/energy transfer, and the subsequent two-fold enhancement in productivity.
Fil: Arnau, Victor Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Rovati, Jose Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Figueroa, L. I. C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XLVI Reunión Anual Sociedad Argentina de Investigaciones Bioquímicas y Moleculares
Puerto Madryn, Chubut, Argentina
Argentina
Sociedad Argentina de Investigaciones Bioquímicas y Moleculares
Materia
ENZIMAS FIBRINOLITICAS
BIONECTRIA SP.
FERMENTACION
DISEÑO FACTORIAL
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/193042
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/193042
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repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Culture Medium Factorial Design Optimization for Fibrinolytic Enzymes Production by Bionectria sp.Arnau, Victor GonzaloRovati, Jose IgnacioFigueroa, L. I. C.Fariña, Julia InesENZIMAS FIBRINOLITICASBIONECTRIA SP.FERMENTACIONDISEÑO FACTORIALhttps://purl.org/becyt/ford/2.9https://purl.org/becyt/ford/2Thrombotic diseases can be clinically treated with fibrinolytic enzymes and many attempts have been made at laboratory level to increase fibrinolytic enzymes production from microbial sources and to reduce the process cost, including culture medium design, optimization of environmental conditions, and over expression with genetically modified strains. In this contribution we present the optimization of culture medium composition and incubation temperature for fibrinolytic enzyme production by Bionectria sp., a selected fungal strain from Las Yungas (Tucumán). Optimization was carried out at Erlenmeyer scale (100-mL working volume) via factorial design methodology. All trials included a common mineral base (%, w/v: NaCl 0.2, KH2PO4 0.05, MgSO4·7H2O 0.05). According to four factorial designs it could be demonstrated the convenience of using soy peptone as N-source, glucose as C-source, and the possibility to eliminate starch, meat peptone and meat extract from original medium composition, whilst 25°C was selected as the optimal incubation temperature. Results showed that culture medium could be successfully optimized by factorial design, achieving a reduction in the production process costs by means of a decrease in culture medium components, the improvement in culture broth rheology, mycelial morphology and mass/energy transfer, and the subsequent two-fold enhancement in productivity.Fil: Arnau, Victor Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Rovati, Jose Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Figueroa, L. I. C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaXLVI Reunión Anual Sociedad Argentina de Investigaciones Bioquímicas y MolecularesPuerto Madryn, Chubut, ArgentinaArgentinaSociedad Argentina de Investigaciones Bioquímicas y MolecularesSociedad Argentina de Investigaciones Bioquímicas y Moleculares2010info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/193042Culture Medium Factorial Design Optimization for Fibrinolytic Enzymes Production by Bionectria sp.; XLVI Reunión Anual Sociedad Argentina de Investigaciones Bioquímicas y Moleculares; Puerto Madryn, Chubut, Argentina; Argentina; 2010; 1-20327-9545CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-34.pdfNacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:30:52Zoai:ri.conicet.gov.ar:11336/193042instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:30:53.024CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Culture Medium Factorial Design Optimization for Fibrinolytic Enzymes Production by Bionectria sp.
title Culture Medium Factorial Design Optimization for Fibrinolytic Enzymes Production by Bionectria sp.
spellingShingle Culture Medium Factorial Design Optimization for Fibrinolytic Enzymes Production by Bionectria sp.
Arnau, Victor Gonzalo
ENZIMAS FIBRINOLITICAS
BIONECTRIA SP.
FERMENTACION
DISEÑO FACTORIAL
title_short Culture Medium Factorial Design Optimization for Fibrinolytic Enzymes Production by Bionectria sp.
title_full Culture Medium Factorial Design Optimization for Fibrinolytic Enzymes Production by Bionectria sp.
title_fullStr Culture Medium Factorial Design Optimization for Fibrinolytic Enzymes Production by Bionectria sp.
title_full_unstemmed Culture Medium Factorial Design Optimization for Fibrinolytic Enzymes Production by Bionectria sp.
title_sort Culture Medium Factorial Design Optimization for Fibrinolytic Enzymes Production by Bionectria sp.
dc.creator.none.fl_str_mv Arnau, Victor Gonzalo
Rovati, Jose Ignacio
Figueroa, L. I. C.
Fariña, Julia Ines
author Arnau, Victor Gonzalo
author_facet Arnau, Victor Gonzalo
Rovati, Jose Ignacio
Figueroa, L. I. C.
Fariña, Julia Ines
author_role author
author2 Rovati, Jose Ignacio
Figueroa, L. I. C.
Fariña, Julia Ines
author2_role author
author
author
dc.subject.none.fl_str_mv ENZIMAS FIBRINOLITICAS
BIONECTRIA SP.
FERMENTACION
DISEÑO FACTORIAL
topic ENZIMAS FIBRINOLITICAS
BIONECTRIA SP.
FERMENTACION
DISEÑO FACTORIAL
purl_subject.fl_str_mv https://purl.org/becyt/ford/2.9
https://purl.org/becyt/ford/2
dc.description.none.fl_txt_mv Thrombotic diseases can be clinically treated with fibrinolytic enzymes and many attempts have been made at laboratory level to increase fibrinolytic enzymes production from microbial sources and to reduce the process cost, including culture medium design, optimization of environmental conditions, and over expression with genetically modified strains. In this contribution we present the optimization of culture medium composition and incubation temperature for fibrinolytic enzyme production by Bionectria sp., a selected fungal strain from Las Yungas (Tucumán). Optimization was carried out at Erlenmeyer scale (100-mL working volume) via factorial design methodology. All trials included a common mineral base (%, w/v: NaCl 0.2, KH2PO4 0.05, MgSO4·7H2O 0.05). According to four factorial designs it could be demonstrated the convenience of using soy peptone as N-source, glucose as C-source, and the possibility to eliminate starch, meat peptone and meat extract from original medium composition, whilst 25°C was selected as the optimal incubation temperature. Results showed that culture medium could be successfully optimized by factorial design, achieving a reduction in the production process costs by means of a decrease in culture medium components, the improvement in culture broth rheology, mycelial morphology and mass/energy transfer, and the subsequent two-fold enhancement in productivity.
Fil: Arnau, Victor Gonzalo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Rovati, Jose Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Figueroa, L. I. C.. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XLVI Reunión Anual Sociedad Argentina de Investigaciones Bioquímicas y Moleculares
Puerto Madryn, Chubut, Argentina
Argentina
Sociedad Argentina de Investigaciones Bioquímicas y Moleculares
description Thrombotic diseases can be clinically treated with fibrinolytic enzymes and many attempts have been made at laboratory level to increase fibrinolytic enzymes production from microbial sources and to reduce the process cost, including culture medium design, optimization of environmental conditions, and over expression with genetically modified strains. In this contribution we present the optimization of culture medium composition and incubation temperature for fibrinolytic enzyme production by Bionectria sp., a selected fungal strain from Las Yungas (Tucumán). Optimization was carried out at Erlenmeyer scale (100-mL working volume) via factorial design methodology. All trials included a common mineral base (%, w/v: NaCl 0.2, KH2PO4 0.05, MgSO4·7H2O 0.05). According to four factorial designs it could be demonstrated the convenience of using soy peptone as N-source, glucose as C-source, and the possibility to eliminate starch, meat peptone and meat extract from original medium composition, whilst 25°C was selected as the optimal incubation temperature. Results showed that culture medium could be successfully optimized by factorial design, achieving a reduction in the production process costs by means of a decrease in culture medium components, the improvement in culture broth rheology, mycelial morphology and mass/energy transfer, and the subsequent two-fold enhancement in productivity.
publishDate 2010
dc.date.none.fl_str_mv 2010
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/193042
Culture Medium Factorial Design Optimization for Fibrinolytic Enzymes Production by Bionectria sp.; XLVI Reunión Anual Sociedad Argentina de Investigaciones Bioquímicas y Moleculares; Puerto Madryn, Chubut, Argentina; Argentina; 2010; 1-2
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/193042
identifier_str_mv Culture Medium Factorial Design Optimization for Fibrinolytic Enzymes Production by Bionectria sp.; XLVI Reunión Anual Sociedad Argentina de Investigaciones Bioquímicas y Moleculares; Puerto Madryn, Chubut, Argentina; Argentina; 2010; 1-2
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-34.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv Sociedad Argentina de Investigaciones Bioquímicas y Moleculares
publisher.none.fl_str_mv Sociedad Argentina de Investigaciones Bioquímicas y Moleculares
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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