Successful fibrinolytic enzymes production by solid state fermentation with a selected Bionectria sp. wild type strain

Autores
Rovati, Jose Ignacio; Cabral, Maria Eugenia; Castellanos, Lucia Ines; Fariña, Julia Ines
Año de publicación
2011
Idioma
español castellano
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Fibrin accumulation in blood vessels increases thrombosis risks promoting myocardial infarction and cardiovascular diseases. Bionectria sp. LY 4.1, a wild fungi isolated from Las Yungas Pedemontana rainforest, was recently reported by our group as a novel and promising fibrinolytic enzyme source. In the present study we attempted the production of fibrinolytic enzymes by solid state fermentation (SSF) with Bionectria sp. LY 4.1 particularly looking for low-cost substrates and simplified operational conditions. The different solid substrates evaluated (polyurethane foam - PUF, bagasse, textured soy flour, soy flour pellets and wheat bran), in adequate amounts, were placed in 500 mL-Erlenmeyer flasks and after sterilization, were inoculated with 10 mL of fresh production medium containing 1 mL of a 72 h-old Bionectria sp. culture. The effect of different conditions of moisture (9, 11, 13, 15, 17%), bed height (1, 2, 4, 6, 8 cm) and particle size (0·25; 0·5; 1 and 2 cm2) was studied. Fibrinolytic activity was determined by the fibrin plate technique and protein concentration by the BCA method. The obtained results confirmed the possibility to produce fibrinolytic enzymes by SSF with Bionectria sp. LY 4.1. Furthermore, assays demonstrated the convenience of using PUF as SS under specific operating conditions over the other substrates tested. The findings herein presented open new frontiers for fungal secondary metabolites production by SSF, allowing to offer an interesting alternative for the production of a high added-value pharmacological product.
Fil: Rovati, Jose Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Cabral, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Potrero de los Funes
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Materia
FIBRINOLYTIC ENZYME
PRODUCTION
SOLID STATE FERMENTATION
BIONECTRIA SP
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/213130
Acceder
id CONICETDig_599e392abd383956fb62440642eb60f2
oai_identifier_str oai:ri.conicet.gov.ar:11336/213130
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Successful fibrinolytic enzymes production by solid state fermentation with a selected Bionectria sp. wild type strainRovati, Jose IgnacioCabral, Maria EugeniaCastellanos, Lucia InesFariña, Julia InesFIBRINOLYTIC ENZYMEPRODUCTIONSOLID STATE FERMENTATIONBIONECTRIA SPhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Fibrin accumulation in blood vessels increases thrombosis risks promoting myocardial infarction and cardiovascular diseases. Bionectria sp. LY 4.1, a wild fungi isolated from Las Yungas Pedemontana rainforest, was recently reported by our group as a novel and promising fibrinolytic enzyme source. In the present study we attempted the production of fibrinolytic enzymes by solid state fermentation (SSF) with Bionectria sp. LY 4.1 particularly looking for low-cost substrates and simplified operational conditions. The different solid substrates evaluated (polyurethane foam - PUF, bagasse, textured soy flour, soy flour pellets and wheat bran), in adequate amounts, were placed in 500 mL-Erlenmeyer flasks and after sterilization, were inoculated with 10 mL of fresh production medium containing 1 mL of a 72 h-old Bionectria sp. culture. The effect of different conditions of moisture (9, 11, 13, 15, 17%), bed height (1, 2, 4, 6, 8 cm) and particle size (0·25; 0·5; 1 and 2 cm2) was studied. Fibrinolytic activity was determined by the fibrin plate technique and protein concentration by the BCA method. The obtained results confirmed the possibility to produce fibrinolytic enzymes by SSF with Bionectria sp. LY 4.1. Furthermore, assays demonstrated the convenience of using PUF as SS under specific operating conditions over the other substrates tested. The findings herein presented open new frontiers for fungal secondary metabolites production by SSF, allowing to offer an interesting alternative for the production of a high added-value pharmacological product.Fil: Rovati, Jose Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Cabral, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaXLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología MolecularPotrero de los FunesArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularTech Science Press2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/213130Successful fibrinolytic enzymes production by solid state fermentation with a selected Bionectria sp. wild type strain; XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Potrero de los Funes; Argentina; 2011; 94-940327-9545CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-35.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T12:02:36Zoai:ri.conicet.gov.ar:11336/213130instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 12:02:37.261CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Successful fibrinolytic enzymes production by solid state fermentation with a selected Bionectria sp. wild type strain
title Successful fibrinolytic enzymes production by solid state fermentation with a selected Bionectria sp. wild type strain
spellingShingle Successful fibrinolytic enzymes production by solid state fermentation with a selected Bionectria sp. wild type strain
Rovati, Jose Ignacio
FIBRINOLYTIC ENZYME
PRODUCTION
SOLID STATE FERMENTATION
BIONECTRIA SP
title_short Successful fibrinolytic enzymes production by solid state fermentation with a selected Bionectria sp. wild type strain
title_full Successful fibrinolytic enzymes production by solid state fermentation with a selected Bionectria sp. wild type strain
title_fullStr Successful fibrinolytic enzymes production by solid state fermentation with a selected Bionectria sp. wild type strain
title_full_unstemmed Successful fibrinolytic enzymes production by solid state fermentation with a selected Bionectria sp. wild type strain
title_sort Successful fibrinolytic enzymes production by solid state fermentation with a selected Bionectria sp. wild type strain
dc.creator.none.fl_str_mv Rovati, Jose Ignacio
Cabral, Maria Eugenia
Castellanos, Lucia Ines
Fariña, Julia Ines
author Rovati, Jose Ignacio
author_facet Rovati, Jose Ignacio
Cabral, Maria Eugenia
Castellanos, Lucia Ines
Fariña, Julia Ines
author_role author
author2 Cabral, Maria Eugenia
Castellanos, Lucia Ines
Fariña, Julia Ines
author2_role author
author
author
dc.subject.none.fl_str_mv FIBRINOLYTIC ENZYME
PRODUCTION
SOLID STATE FERMENTATION
BIONECTRIA SP
topic FIBRINOLYTIC ENZYME
PRODUCTION
SOLID STATE FERMENTATION
BIONECTRIA SP
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Fibrin accumulation in blood vessels increases thrombosis risks promoting myocardial infarction and cardiovascular diseases. Bionectria sp. LY 4.1, a wild fungi isolated from Las Yungas Pedemontana rainforest, was recently reported by our group as a novel and promising fibrinolytic enzyme source. In the present study we attempted the production of fibrinolytic enzymes by solid state fermentation (SSF) with Bionectria sp. LY 4.1 particularly looking for low-cost substrates and simplified operational conditions. The different solid substrates evaluated (polyurethane foam - PUF, bagasse, textured soy flour, soy flour pellets and wheat bran), in adequate amounts, were placed in 500 mL-Erlenmeyer flasks and after sterilization, were inoculated with 10 mL of fresh production medium containing 1 mL of a 72 h-old Bionectria sp. culture. The effect of different conditions of moisture (9, 11, 13, 15, 17%), bed height (1, 2, 4, 6, 8 cm) and particle size (0·25; 0·5; 1 and 2 cm2) was studied. Fibrinolytic activity was determined by the fibrin plate technique and protein concentration by the BCA method. The obtained results confirmed the possibility to produce fibrinolytic enzymes by SSF with Bionectria sp. LY 4.1. Furthermore, assays demonstrated the convenience of using PUF as SS under specific operating conditions over the other substrates tested. The findings herein presented open new frontiers for fungal secondary metabolites production by SSF, allowing to offer an interesting alternative for the production of a high added-value pharmacological product.
Fil: Rovati, Jose Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Cabral, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Potrero de los Funes
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
description Fibrin accumulation in blood vessels increases thrombosis risks promoting myocardial infarction and cardiovascular diseases. Bionectria sp. LY 4.1, a wild fungi isolated from Las Yungas Pedemontana rainforest, was recently reported by our group as a novel and promising fibrinolytic enzyme source. In the present study we attempted the production of fibrinolytic enzymes by solid state fermentation (SSF) with Bionectria sp. LY 4.1 particularly looking for low-cost substrates and simplified operational conditions. The different solid substrates evaluated (polyurethane foam - PUF, bagasse, textured soy flour, soy flour pellets and wheat bran), in adequate amounts, were placed in 500 mL-Erlenmeyer flasks and after sterilization, were inoculated with 10 mL of fresh production medium containing 1 mL of a 72 h-old Bionectria sp. culture. The effect of different conditions of moisture (9, 11, 13, 15, 17%), bed height (1, 2, 4, 6, 8 cm) and particle size (0·25; 0·5; 1 and 2 cm2) was studied. Fibrinolytic activity was determined by the fibrin plate technique and protein concentration by the BCA method. The obtained results confirmed the possibility to produce fibrinolytic enzymes by SSF with Bionectria sp. LY 4.1. Furthermore, assays demonstrated the convenience of using PUF as SS under specific operating conditions over the other substrates tested. The findings herein presented open new frontiers for fungal secondary metabolites production by SSF, allowing to offer an interesting alternative for the production of a high added-value pharmacological product.
publishDate 2011
dc.date.none.fl_str_mv 2011
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/213130
Successful fibrinolytic enzymes production by solid state fermentation with a selected Bionectria sp. wild type strain; XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Potrero de los Funes; Argentina; 2011; 94-94
0327-9545
CONICET Digital
CONICET
url http://hdl.handle.net/11336/213130
identifier_str_mv Successful fibrinolytic enzymes production by solid state fermentation with a selected Bionectria sp. wild type strain; XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Potrero de los Funes; Argentina; 2011; 94-94
0327-9545
CONICET Digital
CONICET
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-35.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv Tech Science Press
publisher.none.fl_str_mv Tech Science Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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