Successful fibrinolytic enzymes production by solid state fermentation with a selected Bionectria sp. wild type strain
- Autores
- Rovati, Jose Ignacio; Cabral, Maria Eugenia; Castellanos, Lucia Ines; Fariña, Julia Ines
- Año de publicación
- 2011
- Idioma
- español castellano
- Tipo de recurso
- documento de conferencia
- Estado
- versión publicada
- Descripción
- Fibrin accumulation in blood vessels increases thrombosis risks promoting myocardial infarction and cardiovascular diseases. Bionectria sp. LY 4.1, a wild fungi isolated from Las Yungas Pedemontana rainforest, was recently reported by our group as a novel and promising fibrinolytic enzyme source. In the present study we attempted the production of fibrinolytic enzymes by solid state fermentation (SSF) with Bionectria sp. LY 4.1 particularly looking for low-cost substrates and simplified operational conditions. The different solid substrates evaluated (polyurethane foam - PUF, bagasse, textured soy flour, soy flour pellets and wheat bran), in adequate amounts, were placed in 500 mL-Erlenmeyer flasks and after sterilization, were inoculated with 10 mL of fresh production medium containing 1 mL of a 72 h-old Bionectria sp. culture. The effect of different conditions of moisture (9, 11, 13, 15, 17%), bed height (1, 2, 4, 6, 8 cm) and particle size (0·25; 0·5; 1 and 2 cm2) was studied. Fibrinolytic activity was determined by the fibrin plate technique and protein concentration by the BCA method. The obtained results confirmed the possibility to produce fibrinolytic enzymes by SSF with Bionectria sp. LY 4.1. Furthermore, assays demonstrated the convenience of using PUF as SS under specific operating conditions over the other substrates tested. The findings herein presented open new frontiers for fungal secondary metabolites production by SSF, allowing to offer an interesting alternative for the production of a high added-value pharmacological product.
Fil: Rovati, Jose Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Cabral, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina
XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular
Potrero de los Funes
Argentina
Sociedad Argentina de Investigación en Bioquímica y Biología Molecular - Materia
-
FIBRINOLYTIC ENZYME
PRODUCTION
SOLID STATE FERMENTATION
BIONECTRIA SP - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/213130
Ver los metadatos del registro completo
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Successful fibrinolytic enzymes production by solid state fermentation with a selected Bionectria sp. wild type strainRovati, Jose IgnacioCabral, Maria EugeniaCastellanos, Lucia InesFariña, Julia InesFIBRINOLYTIC ENZYMEPRODUCTIONSOLID STATE FERMENTATIONBIONECTRIA SPhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Fibrin accumulation in blood vessels increases thrombosis risks promoting myocardial infarction and cardiovascular diseases. Bionectria sp. LY 4.1, a wild fungi isolated from Las Yungas Pedemontana rainforest, was recently reported by our group as a novel and promising fibrinolytic enzyme source. In the present study we attempted the production of fibrinolytic enzymes by solid state fermentation (SSF) with Bionectria sp. LY 4.1 particularly looking for low-cost substrates and simplified operational conditions. The different solid substrates evaluated (polyurethane foam - PUF, bagasse, textured soy flour, soy flour pellets and wheat bran), in adequate amounts, were placed in 500 mL-Erlenmeyer flasks and after sterilization, were inoculated with 10 mL of fresh production medium containing 1 mL of a 72 h-old Bionectria sp. culture. The effect of different conditions of moisture (9, 11, 13, 15, 17%), bed height (1, 2, 4, 6, 8 cm) and particle size (0·25; 0·5; 1 and 2 cm2) was studied. Fibrinolytic activity was determined by the fibrin plate technique and protein concentration by the BCA method. The obtained results confirmed the possibility to produce fibrinolytic enzymes by SSF with Bionectria sp. LY 4.1. Furthermore, assays demonstrated the convenience of using PUF as SS under specific operating conditions over the other substrates tested. The findings herein presented open new frontiers for fungal secondary metabolites production by SSF, allowing to offer an interesting alternative for the production of a high added-value pharmacological product.Fil: Rovati, Jose Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Cabral, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaFil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; ArgentinaXLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología MolecularPotrero de los FunesArgentinaSociedad Argentina de Investigación en Bioquímica y Biología MolecularTech Science Press2011info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/213130Successful fibrinolytic enzymes production by solid state fermentation with a selected Bionectria sp. wild type strain; XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Potrero de los Funes; Argentina; 2011; 94-940327-9545CONICET DigitalCONICETspainfo:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-35.pdfInternacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:30:02Zoai:ri.conicet.gov.ar:11336/213130instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:30:02.501CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
Successful fibrinolytic enzymes production by solid state fermentation with a selected Bionectria sp. wild type strain |
title |
Successful fibrinolytic enzymes production by solid state fermentation with a selected Bionectria sp. wild type strain |
spellingShingle |
Successful fibrinolytic enzymes production by solid state fermentation with a selected Bionectria sp. wild type strain Rovati, Jose Ignacio FIBRINOLYTIC ENZYME PRODUCTION SOLID STATE FERMENTATION BIONECTRIA SP |
title_short |
Successful fibrinolytic enzymes production by solid state fermentation with a selected Bionectria sp. wild type strain |
title_full |
Successful fibrinolytic enzymes production by solid state fermentation with a selected Bionectria sp. wild type strain |
title_fullStr |
Successful fibrinolytic enzymes production by solid state fermentation with a selected Bionectria sp. wild type strain |
title_full_unstemmed |
Successful fibrinolytic enzymes production by solid state fermentation with a selected Bionectria sp. wild type strain |
title_sort |
Successful fibrinolytic enzymes production by solid state fermentation with a selected Bionectria sp. wild type strain |
dc.creator.none.fl_str_mv |
Rovati, Jose Ignacio Cabral, Maria Eugenia Castellanos, Lucia Ines Fariña, Julia Ines |
author |
Rovati, Jose Ignacio |
author_facet |
Rovati, Jose Ignacio Cabral, Maria Eugenia Castellanos, Lucia Ines Fariña, Julia Ines |
author_role |
author |
author2 |
Cabral, Maria Eugenia Castellanos, Lucia Ines Fariña, Julia Ines |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
FIBRINOLYTIC ENZYME PRODUCTION SOLID STATE FERMENTATION BIONECTRIA SP |
topic |
FIBRINOLYTIC ENZYME PRODUCTION SOLID STATE FERMENTATION BIONECTRIA SP |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/1.6 https://purl.org/becyt/ford/1 |
dc.description.none.fl_txt_mv |
Fibrin accumulation in blood vessels increases thrombosis risks promoting myocardial infarction and cardiovascular diseases. Bionectria sp. LY 4.1, a wild fungi isolated from Las Yungas Pedemontana rainforest, was recently reported by our group as a novel and promising fibrinolytic enzyme source. In the present study we attempted the production of fibrinolytic enzymes by solid state fermentation (SSF) with Bionectria sp. LY 4.1 particularly looking for low-cost substrates and simplified operational conditions. The different solid substrates evaluated (polyurethane foam - PUF, bagasse, textured soy flour, soy flour pellets and wheat bran), in adequate amounts, were placed in 500 mL-Erlenmeyer flasks and after sterilization, were inoculated with 10 mL of fresh production medium containing 1 mL of a 72 h-old Bionectria sp. culture. The effect of different conditions of moisture (9, 11, 13, 15, 17%), bed height (1, 2, 4, 6, 8 cm) and particle size (0·25; 0·5; 1 and 2 cm2) was studied. Fibrinolytic activity was determined by the fibrin plate technique and protein concentration by the BCA method. The obtained results confirmed the possibility to produce fibrinolytic enzymes by SSF with Bionectria sp. LY 4.1. Furthermore, assays demonstrated the convenience of using PUF as SS under specific operating conditions over the other substrates tested. The findings herein presented open new frontiers for fungal secondary metabolites production by SSF, allowing to offer an interesting alternative for the production of a high added-value pharmacological product. Fil: Rovati, Jose Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Cabral, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Castellanos, Lucia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina Fil: Fariña, Julia Ines. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Planta Piloto de Procesos Industriales Microbiológicos; Argentina XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular Potrero de los Funes Argentina Sociedad Argentina de Investigación en Bioquímica y Biología Molecular |
description |
Fibrin accumulation in blood vessels increases thrombosis risks promoting myocardial infarction and cardiovascular diseases. Bionectria sp. LY 4.1, a wild fungi isolated from Las Yungas Pedemontana rainforest, was recently reported by our group as a novel and promising fibrinolytic enzyme source. In the present study we attempted the production of fibrinolytic enzymes by solid state fermentation (SSF) with Bionectria sp. LY 4.1 particularly looking for low-cost substrates and simplified operational conditions. The different solid substrates evaluated (polyurethane foam - PUF, bagasse, textured soy flour, soy flour pellets and wheat bran), in adequate amounts, were placed in 500 mL-Erlenmeyer flasks and after sterilization, were inoculated with 10 mL of fresh production medium containing 1 mL of a 72 h-old Bionectria sp. culture. The effect of different conditions of moisture (9, 11, 13, 15, 17%), bed height (1, 2, 4, 6, 8 cm) and particle size (0·25; 0·5; 1 and 2 cm2) was studied. Fibrinolytic activity was determined by the fibrin plate technique and protein concentration by the BCA method. The obtained results confirmed the possibility to produce fibrinolytic enzymes by SSF with Bionectria sp. LY 4.1. Furthermore, assays demonstrated the convenience of using PUF as SS under specific operating conditions over the other substrates tested. The findings herein presented open new frontiers for fungal secondary metabolites production by SSF, allowing to offer an interesting alternative for the production of a high added-value pharmacological product. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/conferenceObject Reunión Journal http://purl.org/coar/resource_type/c_5794 info:ar-repo/semantics/documentoDeConferencia |
status_str |
publishedVersion |
format |
conferenceObject |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/213130 Successful fibrinolytic enzymes production by solid state fermentation with a selected Bionectria sp. wild type strain; XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Potrero de los Funes; Argentina; 2011; 94-94 0327-9545 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/213130 |
identifier_str_mv |
Successful fibrinolytic enzymes production by solid state fermentation with a selected Bionectria sp. wild type strain; XLVII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; Potrero de los Funes; Argentina; 2011; 94-94 0327-9545 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
spa |
language |
spa |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/url/https://saib.org.ar/archivos/biocell-35.pdf |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by/2.5/ar/ |
dc.format.none.fl_str_mv |
application/pdf application/pdf application/pdf |
dc.coverage.none.fl_str_mv |
Internacional |
dc.publisher.none.fl_str_mv |
Tech Science Press |
publisher.none.fl_str_mv |
Tech Science Press |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
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dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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