ATF3 is a novel nuclear marker for migrating ependymal stem cells in the rat spinal cord

Autores
Mladinic, Miranda; Bianchetti, Elena; Dekanic, Ana; Mazzone, Graciela Luján; Nistri, Andrea
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The present study identified ATF3 as a novel dynamic marker for ependymal stem/progenitor cells (nestin, vimentin and SOX2 positive) around the central canal of the neonatal or adult rat spinal cord. While quiescent ependymal cells showed cytoplasmic ATF3 expression, during 6?24 h in vitro these cells mobilized and acquired intense nuclear ATF3 staining. Their migratory pattern followed a centrifugal pathway toward the dorsal and ventral funiculi, reminiscent of the rostral migratory stream of the brain subventricular stem cells. Thus, the chain cell formation was, by analogy, termed funicular migratory stream (FMS). The FMS process preceded the strong proliferation of ependymal cells occurring only after 24 h in vitro. Pharmacological inhibition of MAPK-p38 and JNK/c-Jun (upstream effectors of ATF3 activation) prevented the FMS mobilization of ATF3 nuclear-positive cells. Excitotoxicity or ischemia-like conditions, reported to evoke neuronal and glial injury, did not further enhance migration of ependymal cells at 24 h, suggesting that, at this early stage of damage, the FMS phenomenon had peaked and that more extensive repair processes are delayed beyond this time point. ATF3 is, therefore, useful to identify activation and migration of endogenous stem cells of the rat spinal cord in vitro.
Fil: Mladinic, Miranda. Scuola Internazionale Superiore di Studi Avanzati; Italia. University of Rijeka. Department of Biotechnology; Croacia. Istituto di Medicina Fisica e Riabilitazione; Italia
Fil: Bianchetti, Elena. Scuola Internazionale Superiore di Studi Avanzati; Italia
Fil: Dekanic, Ana. University of Rijeka. Department of Biotechnology; Croacia. Scuola Internazionale Superiore di Studi Avanzati; Italia
Fil: Mazzone, Graciela Luján. Scuola Internazionale Superiore di Studi Avanzati; Italia
Fil: Nistri, Andrea. Scuola Internazionale Superiore di Studi Avanzati; Italia. Istituto di Medicina Fisica e Riabilitazione; Italia
Materia
ATF3
Ependymal stem cells
Spinal cord
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/33654
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling ATF3 is a novel nuclear marker for migrating ependymal stem cells in the rat spinal cordMladinic, MirandaBianchetti, ElenaDekanic, AnaMazzone, Graciela LujánNistri, AndreaATF3Ependymal stem cellsSpinal cordhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3The present study identified ATF3 as a novel dynamic marker for ependymal stem/progenitor cells (nestin, vimentin and SOX2 positive) around the central canal of the neonatal or adult rat spinal cord. While quiescent ependymal cells showed cytoplasmic ATF3 expression, during 6?24 h in vitro these cells mobilized and acquired intense nuclear ATF3 staining. Their migratory pattern followed a centrifugal pathway toward the dorsal and ventral funiculi, reminiscent of the rostral migratory stream of the brain subventricular stem cells. Thus, the chain cell formation was, by analogy, termed funicular migratory stream (FMS). The FMS process preceded the strong proliferation of ependymal cells occurring only after 24 h in vitro. Pharmacological inhibition of MAPK-p38 and JNK/c-Jun (upstream effectors of ATF3 activation) prevented the FMS mobilization of ATF3 nuclear-positive cells. Excitotoxicity or ischemia-like conditions, reported to evoke neuronal and glial injury, did not further enhance migration of ependymal cells at 24 h, suggesting that, at this early stage of damage, the FMS phenomenon had peaked and that more extensive repair processes are delayed beyond this time point. ATF3 is, therefore, useful to identify activation and migration of endogenous stem cells of the rat spinal cord in vitro.Fil: Mladinic, Miranda. Scuola Internazionale Superiore di Studi Avanzati; Italia. University of Rijeka. Department of Biotechnology; Croacia. Istituto di Medicina Fisica e Riabilitazione; ItaliaFil: Bianchetti, Elena. Scuola Internazionale Superiore di Studi Avanzati; ItaliaFil: Dekanic, Ana. University of Rijeka. Department of Biotechnology; Croacia. Scuola Internazionale Superiore di Studi Avanzati; ItaliaFil: Mazzone, Graciela Luján. Scuola Internazionale Superiore di Studi Avanzati; ItaliaFil: Nistri, Andrea. Scuola Internazionale Superiore di Studi Avanzati; Italia. Istituto di Medicina Fisica e Riabilitazione; ItaliaElsevier2014-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/33654Bianchetti, Elena; Dekanic, Ana; Mazzone, Graciela Luján; Nistri, Andrea; Mladinic, Miranda; ATF3 is a novel nuclear marker for migrating ependymal stem cells in the rat spinal cord; Elsevier; Stem Cell Research; 13; 3; 3-2014; 815-8271873-5061CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1873506114000336info:eu-repo/semantics/altIdentifier/doi/10.1016/j.scr.2014.03.006info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-10T13:07:37Zoai:ri.conicet.gov.ar:11336/33654instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-10 13:07:37.368CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv ATF3 is a novel nuclear marker for migrating ependymal stem cells in the rat spinal cord
title ATF3 is a novel nuclear marker for migrating ependymal stem cells in the rat spinal cord
spellingShingle ATF3 is a novel nuclear marker for migrating ependymal stem cells in the rat spinal cord
Mladinic, Miranda
ATF3
Ependymal stem cells
Spinal cord
title_short ATF3 is a novel nuclear marker for migrating ependymal stem cells in the rat spinal cord
title_full ATF3 is a novel nuclear marker for migrating ependymal stem cells in the rat spinal cord
title_fullStr ATF3 is a novel nuclear marker for migrating ependymal stem cells in the rat spinal cord
title_full_unstemmed ATF3 is a novel nuclear marker for migrating ependymal stem cells in the rat spinal cord
title_sort ATF3 is a novel nuclear marker for migrating ependymal stem cells in the rat spinal cord
dc.creator.none.fl_str_mv Mladinic, Miranda
Bianchetti, Elena
Dekanic, Ana
Mazzone, Graciela Luján
Nistri, Andrea
author Mladinic, Miranda
author_facet Mladinic, Miranda
Bianchetti, Elena
Dekanic, Ana
Mazzone, Graciela Luján
Nistri, Andrea
author_role author
author2 Bianchetti, Elena
Dekanic, Ana
Mazzone, Graciela Luján
Nistri, Andrea
author2_role author
author
author
author
dc.subject.none.fl_str_mv ATF3
Ependymal stem cells
Spinal cord
topic ATF3
Ependymal stem cells
Spinal cord
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv The present study identified ATF3 as a novel dynamic marker for ependymal stem/progenitor cells (nestin, vimentin and SOX2 positive) around the central canal of the neonatal or adult rat spinal cord. While quiescent ependymal cells showed cytoplasmic ATF3 expression, during 6?24 h in vitro these cells mobilized and acquired intense nuclear ATF3 staining. Their migratory pattern followed a centrifugal pathway toward the dorsal and ventral funiculi, reminiscent of the rostral migratory stream of the brain subventricular stem cells. Thus, the chain cell formation was, by analogy, termed funicular migratory stream (FMS). The FMS process preceded the strong proliferation of ependymal cells occurring only after 24 h in vitro. Pharmacological inhibition of MAPK-p38 and JNK/c-Jun (upstream effectors of ATF3 activation) prevented the FMS mobilization of ATF3 nuclear-positive cells. Excitotoxicity or ischemia-like conditions, reported to evoke neuronal and glial injury, did not further enhance migration of ependymal cells at 24 h, suggesting that, at this early stage of damage, the FMS phenomenon had peaked and that more extensive repair processes are delayed beyond this time point. ATF3 is, therefore, useful to identify activation and migration of endogenous stem cells of the rat spinal cord in vitro.
Fil: Mladinic, Miranda. Scuola Internazionale Superiore di Studi Avanzati; Italia. University of Rijeka. Department of Biotechnology; Croacia. Istituto di Medicina Fisica e Riabilitazione; Italia
Fil: Bianchetti, Elena. Scuola Internazionale Superiore di Studi Avanzati; Italia
Fil: Dekanic, Ana. University of Rijeka. Department of Biotechnology; Croacia. Scuola Internazionale Superiore di Studi Avanzati; Italia
Fil: Mazzone, Graciela Luján. Scuola Internazionale Superiore di Studi Avanzati; Italia
Fil: Nistri, Andrea. Scuola Internazionale Superiore di Studi Avanzati; Italia. Istituto di Medicina Fisica e Riabilitazione; Italia
description The present study identified ATF3 as a novel dynamic marker for ependymal stem/progenitor cells (nestin, vimentin and SOX2 positive) around the central canal of the neonatal or adult rat spinal cord. While quiescent ependymal cells showed cytoplasmic ATF3 expression, during 6?24 h in vitro these cells mobilized and acquired intense nuclear ATF3 staining. Their migratory pattern followed a centrifugal pathway toward the dorsal and ventral funiculi, reminiscent of the rostral migratory stream of the brain subventricular stem cells. Thus, the chain cell formation was, by analogy, termed funicular migratory stream (FMS). The FMS process preceded the strong proliferation of ependymal cells occurring only after 24 h in vitro. Pharmacological inhibition of MAPK-p38 and JNK/c-Jun (upstream effectors of ATF3 activation) prevented the FMS mobilization of ATF3 nuclear-positive cells. Excitotoxicity or ischemia-like conditions, reported to evoke neuronal and glial injury, did not further enhance migration of ependymal cells at 24 h, suggesting that, at this early stage of damage, the FMS phenomenon had peaked and that more extensive repair processes are delayed beyond this time point. ATF3 is, therefore, useful to identify activation and migration of endogenous stem cells of the rat spinal cord in vitro.
publishDate 2014
dc.date.none.fl_str_mv 2014-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/33654
Bianchetti, Elena; Dekanic, Ana; Mazzone, Graciela Luján; Nistri, Andrea; Mladinic, Miranda; ATF3 is a novel nuclear marker for migrating ependymal stem cells in the rat spinal cord; Elsevier; Stem Cell Research; 13; 3; 3-2014; 815-827
1873-5061
CONICET Digital
CONICET
url http://hdl.handle.net/11336/33654
identifier_str_mv Bianchetti, Elena; Dekanic, Ana; Mazzone, Graciela Luján; Nistri, Andrea; Mladinic, Miranda; ATF3 is a novel nuclear marker for migrating ependymal stem cells in the rat spinal cord; Elsevier; Stem Cell Research; 13; 3; 3-2014; 815-827
1873-5061
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1873506114000336
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.scr.2014.03.006
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.993085

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