SlSPA3 regulates the nuclear abundance of SlUVR8 in tomato

Autores
Zhang, Qianwen; Liu, Yue; Zhang, Chunli; Xu, Dawei; Medina Fraga, Ana Laura; Wu, Baoguo; Guo, Chenyang; Wangzha, MeLongying; Yang, Guoqian; Zhu, Danmeng; Weiss, David; Ballare, Carlos Luis; Lin, Li; Yin, Ruohe
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Tomato (Solanum lycopersicum L.) is an important model plant species in photomorphogenesis research. Ultraviolet B (UV-B) induces the dissociation of homodimers of the photoreceptor UV RESISTANCE LOCUS8 (UVR8) into monomers, which translocate into the nucleus. Nuclear accumulation of UVR8 is a prerequisite for its signaling function. Previous studies have reported that SUPPRESSOR OF PHYTOCHROME A-105 (SPA) family members may regulate UV-B signaling in Arabidopsis (Arabidopsis thaliana); however, the underlying mechanism is unknown. Here, we show that the tomato genome encodes four SPA (SlSPA) orthologs. Genome-edited Slspa3 mutants exhibited enhanced photomorphogenic responses in white light, suggesting that SlSPA3 inhibits general photomorphogenesis. By contrast, UVR8-mediated gene expression in response to UV-B was compromised in Slspa3 mutants, suggesting that SlSPA3 promotes UV-B signaling. UV-B-induced nuclear accumulation of UVR8, which is essential for UV-B signaling, was reduced in the Slspa3 mutants. Moreover, UV-B-induced nuclear accumulation of UVR8 was also reduced in the Arabidopsis spa1 spa2 spa3 and spa1 spa2 spa4 triple mutants, indicating a conserved mechanism in these two species. Notably, spa1 spa2 spa4 exhibited normal UV-B-induced interaction between UVR8 and the plant morphogenesis repressor CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1). This suggests that the well-established mechanisms of UVR8 nuclear retention remained unaffected in spa1 spa2 spa4. Thus, our work uncovered a potentially unrecognized mechanism by which SPA proteins regulate UV-B signaling through the promotion of UVR8 nuclear abundance in land plants.
Fil: Zhang, Qianwen. Shanghai Jiao Tong University; China. Hubei University of Chinese Medicine; China
Fil: Liu, Yue. Shanghai Jiao Tong University; China
Fil: Zhang, Chunli. Shanghai Jiao Tong University; China
Fil: Xu, Dawei. Shanghai Jiao Tong University; China
Fil: Medina Fraga, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Wu, Baoguo. Shanghai Jiao Tong University; China
Fil: Guo, Chenyang. Shanghai Jiao Tong University; China
Fil: Wangzha, MeLongying. Shanghai Jiao Tong University; China
Fil: Yang, Guoqian. Shanghai Jiao Tong University; China
Fil: Zhu, Danmeng. Peking University; China
Fil: Weiss, David. University of Jerusalem; Israel
Fil: Ballare, Carlos Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Lin, Li. Shanghai Jiao Tong University; China
Fil: Yin, Ruohe. Shanghai Jiao Tong University; China
Materia
Solanum lycopersicum
UV-B
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/266237
Acceder
id CONICETDig_97f07f7a50743c9e59047e0629e587ea
oai_identifier_str oai:ri.conicet.gov.ar:11336/266237
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling SlSPA3 regulates the nuclear abundance of SlUVR8 in tomatoZhang, QianwenLiu, YueZhang, ChunliXu, DaweiMedina Fraga, Ana LauraWu, BaoguoGuo, ChenyangWangzha, MeLongyingYang, GuoqianZhu, DanmengWeiss, DavidBallare, Carlos LuisLin, LiYin, RuoheSolanum lycopersicumUV-Bhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Tomato (Solanum lycopersicum L.) is an important model plant species in photomorphogenesis research. Ultraviolet B (UV-B) induces the dissociation of homodimers of the photoreceptor UV RESISTANCE LOCUS8 (UVR8) into monomers, which translocate into the nucleus. Nuclear accumulation of UVR8 is a prerequisite for its signaling function. Previous studies have reported that SUPPRESSOR OF PHYTOCHROME A-105 (SPA) family members may regulate UV-B signaling in Arabidopsis (Arabidopsis thaliana); however, the underlying mechanism is unknown. Here, we show that the tomato genome encodes four SPA (SlSPA) orthologs. Genome-edited Slspa3 mutants exhibited enhanced photomorphogenic responses in white light, suggesting that SlSPA3 inhibits general photomorphogenesis. By contrast, UVR8-mediated gene expression in response to UV-B was compromised in Slspa3 mutants, suggesting that SlSPA3 promotes UV-B signaling. UV-B-induced nuclear accumulation of UVR8, which is essential for UV-B signaling, was reduced in the Slspa3 mutants. Moreover, UV-B-induced nuclear accumulation of UVR8 was also reduced in the Arabidopsis spa1 spa2 spa3 and spa1 spa2 spa4 triple mutants, indicating a conserved mechanism in these two species. Notably, spa1 spa2 spa4 exhibited normal UV-B-induced interaction between UVR8 and the plant morphogenesis repressor CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1). This suggests that the well-established mechanisms of UVR8 nuclear retention remained unaffected in spa1 spa2 spa4. Thus, our work uncovered a potentially unrecognized mechanism by which SPA proteins regulate UV-B signaling through the promotion of UVR8 nuclear abundance in land plants.Fil: Zhang, Qianwen. Shanghai Jiao Tong University; China. Hubei University of Chinese Medicine; ChinaFil: Liu, Yue. Shanghai Jiao Tong University; ChinaFil: Zhang, Chunli. Shanghai Jiao Tong University; ChinaFil: Xu, Dawei. Shanghai Jiao Tong University; ChinaFil: Medina Fraga, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaFil: Wu, Baoguo. Shanghai Jiao Tong University; ChinaFil: Guo, Chenyang. Shanghai Jiao Tong University; ChinaFil: Wangzha, MeLongying. Shanghai Jiao Tong University; ChinaFil: Yang, Guoqian. Shanghai Jiao Tong University; ChinaFil: Zhu, Danmeng. Peking University; ChinaFil: Weiss, David. University of Jerusalem; IsraelFil: Ballare, Carlos Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; ArgentinaFil: Lin, Li. Shanghai Jiao Tong University; ChinaFil: Yin, Ruohe. Shanghai Jiao Tong University; ChinaWiley Blackwell Publishing, Inc2024-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/266237Zhang, Qianwen; Liu, Yue; Zhang, Chunli; Xu, Dawei; Medina Fraga, Ana Laura; et al.; SlSPA3 regulates the nuclear abundance of SlUVR8 in tomato; Wiley Blackwell Publishing, Inc; Plant Journal; 120; 6; 11-2024; 2656-26670960-7412CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/tpj.17135info:eu-repo/semantics/altIdentifier/doi/10.1111/tpj.17135info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:43:29Zoai:ri.conicet.gov.ar:11336/266237instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:43:29.894CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv SlSPA3 regulates the nuclear abundance of SlUVR8 in tomato
title SlSPA3 regulates the nuclear abundance of SlUVR8 in tomato
spellingShingle SlSPA3 regulates the nuclear abundance of SlUVR8 in tomato
Zhang, Qianwen
Solanum lycopersicum
UV-B
title_short SlSPA3 regulates the nuclear abundance of SlUVR8 in tomato
title_full SlSPA3 regulates the nuclear abundance of SlUVR8 in tomato
title_fullStr SlSPA3 regulates the nuclear abundance of SlUVR8 in tomato
title_full_unstemmed SlSPA3 regulates the nuclear abundance of SlUVR8 in tomato
title_sort SlSPA3 regulates the nuclear abundance of SlUVR8 in tomato
dc.creator.none.fl_str_mv Zhang, Qianwen
Liu, Yue
Zhang, Chunli
Xu, Dawei
Medina Fraga, Ana Laura
Wu, Baoguo
Guo, Chenyang
Wangzha, MeLongying
Yang, Guoqian
Zhu, Danmeng
Weiss, David
Ballare, Carlos Luis
Lin, Li
Yin, Ruohe
author Zhang, Qianwen
author_facet Zhang, Qianwen
Liu, Yue
Zhang, Chunli
Xu, Dawei
Medina Fraga, Ana Laura
Wu, Baoguo
Guo, Chenyang
Wangzha, MeLongying
Yang, Guoqian
Zhu, Danmeng
Weiss, David
Ballare, Carlos Luis
Lin, Li
Yin, Ruohe
author_role author
author2 Liu, Yue
Zhang, Chunli
Xu, Dawei
Medina Fraga, Ana Laura
Wu, Baoguo
Guo, Chenyang
Wangzha, MeLongying
Yang, Guoqian
Zhu, Danmeng
Weiss, David
Ballare, Carlos Luis
Lin, Li
Yin, Ruohe
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Solanum lycopersicum
UV-B
topic Solanum lycopersicum
UV-B
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Tomato (Solanum lycopersicum L.) is an important model plant species in photomorphogenesis research. Ultraviolet B (UV-B) induces the dissociation of homodimers of the photoreceptor UV RESISTANCE LOCUS8 (UVR8) into monomers, which translocate into the nucleus. Nuclear accumulation of UVR8 is a prerequisite for its signaling function. Previous studies have reported that SUPPRESSOR OF PHYTOCHROME A-105 (SPA) family members may regulate UV-B signaling in Arabidopsis (Arabidopsis thaliana); however, the underlying mechanism is unknown. Here, we show that the tomato genome encodes four SPA (SlSPA) orthologs. Genome-edited Slspa3 mutants exhibited enhanced photomorphogenic responses in white light, suggesting that SlSPA3 inhibits general photomorphogenesis. By contrast, UVR8-mediated gene expression in response to UV-B was compromised in Slspa3 mutants, suggesting that SlSPA3 promotes UV-B signaling. UV-B-induced nuclear accumulation of UVR8, which is essential for UV-B signaling, was reduced in the Slspa3 mutants. Moreover, UV-B-induced nuclear accumulation of UVR8 was also reduced in the Arabidopsis spa1 spa2 spa3 and spa1 spa2 spa4 triple mutants, indicating a conserved mechanism in these two species. Notably, spa1 spa2 spa4 exhibited normal UV-B-induced interaction between UVR8 and the plant morphogenesis repressor CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1). This suggests that the well-established mechanisms of UVR8 nuclear retention remained unaffected in spa1 spa2 spa4. Thus, our work uncovered a potentially unrecognized mechanism by which SPA proteins regulate UV-B signaling through the promotion of UVR8 nuclear abundance in land plants.
Fil: Zhang, Qianwen. Shanghai Jiao Tong University; China. Hubei University of Chinese Medicine; China
Fil: Liu, Yue. Shanghai Jiao Tong University; China
Fil: Zhang, Chunli. Shanghai Jiao Tong University; China
Fil: Xu, Dawei. Shanghai Jiao Tong University; China
Fil: Medina Fraga, Ana Laura. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Wu, Baoguo. Shanghai Jiao Tong University; China
Fil: Guo, Chenyang. Shanghai Jiao Tong University; China
Fil: Wangzha, MeLongying. Shanghai Jiao Tong University; China
Fil: Yang, Guoqian. Shanghai Jiao Tong University; China
Fil: Zhu, Danmeng. Peking University; China
Fil: Weiss, David. University of Jerusalem; Israel
Fil: Ballare, Carlos Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura. Universidad de Buenos Aires. Facultad de Agronomía. Instituto de Investigaciones Fisiológicas y Ecológicas Vinculadas a la Agricultura; Argentina
Fil: Lin, Li. Shanghai Jiao Tong University; China
Fil: Yin, Ruohe. Shanghai Jiao Tong University; China
description Tomato (Solanum lycopersicum L.) is an important model plant species in photomorphogenesis research. Ultraviolet B (UV-B) induces the dissociation of homodimers of the photoreceptor UV RESISTANCE LOCUS8 (UVR8) into monomers, which translocate into the nucleus. Nuclear accumulation of UVR8 is a prerequisite for its signaling function. Previous studies have reported that SUPPRESSOR OF PHYTOCHROME A-105 (SPA) family members may regulate UV-B signaling in Arabidopsis (Arabidopsis thaliana); however, the underlying mechanism is unknown. Here, we show that the tomato genome encodes four SPA (SlSPA) orthologs. Genome-edited Slspa3 mutants exhibited enhanced photomorphogenic responses in white light, suggesting that SlSPA3 inhibits general photomorphogenesis. By contrast, UVR8-mediated gene expression in response to UV-B was compromised in Slspa3 mutants, suggesting that SlSPA3 promotes UV-B signaling. UV-B-induced nuclear accumulation of UVR8, which is essential for UV-B signaling, was reduced in the Slspa3 mutants. Moreover, UV-B-induced nuclear accumulation of UVR8 was also reduced in the Arabidopsis spa1 spa2 spa3 and spa1 spa2 spa4 triple mutants, indicating a conserved mechanism in these two species. Notably, spa1 spa2 spa4 exhibited normal UV-B-induced interaction between UVR8 and the plant morphogenesis repressor CONSTITUTIVE PHOTOMORPHOGENIC 1 (COP1). This suggests that the well-established mechanisms of UVR8 nuclear retention remained unaffected in spa1 spa2 spa4. Thus, our work uncovered a potentially unrecognized mechanism by which SPA proteins regulate UV-B signaling through the promotion of UVR8 nuclear abundance in land plants.
publishDate 2024
dc.date.none.fl_str_mv 2024-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/266237
Zhang, Qianwen; Liu, Yue; Zhang, Chunli; Xu, Dawei; Medina Fraga, Ana Laura; et al.; SlSPA3 regulates the nuclear abundance of SlUVR8 in tomato; Wiley Blackwell Publishing, Inc; Plant Journal; 120; 6; 11-2024; 2656-2667
0960-7412
CONICET Digital
CONICET
url http://hdl.handle.net/11336/266237
identifier_str_mv Zhang, Qianwen; Liu, Yue; Zhang, Chunli; Xu, Dawei; Medina Fraga, Ana Laura; et al.; SlSPA3 regulates the nuclear abundance of SlUVR8 in tomato; Wiley Blackwell Publishing, Inc; Plant Journal; 120; 6; 11-2024; 2656-2667
0960-7412
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/10.1111/tpj.17135
info:eu-repo/semantics/altIdentifier/doi/10.1111/tpj.17135
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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