Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms

Autores
Salimena, Alessandra P. S.; Lange, Carla C.; Camussone, Cecilia María; Signorini Porchietto, Marcelo Lisandro; Calvinho, Luis Fernando; Brito, Maria A. V. P.; Borges, Cristiano A. V.; Guimarães, Alessandro S.; Ribeiro, João B.; Mendonça, Letícia C.; Piccoli, Roberta H.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Staphylococcus aureus is a pathogen that frequently causes mastitis in bovine herds worldwide. This pathogen produces several virulence factors, including cell-associated adhesins, toxic and cytolytic exoproteins, and capsular polysaccharides. The aim of the present study was to test for the presence of genes involved in capsular polysaccharide production and biofilm formation in S. aureus isolated from bovine mastitis samples collected from 119 dairy herds located in three different Brazilian regions, as well as to assay the production of capsular polysaccharides and biofilm, in vitro. The detection of the cap, icaAD, and bap genes was performed using PCR. The detection and quantification of capsular polysaccharide production was performed using ELISA assays. The ability of the isolates to form a biofilm was examined using the polystyrene surface of microtiter plates. All 159 S. aureus isolates investigated harboured the cap gene: 80 % carried the cap5 gene and 20 % carried the cap8 gene. Sixty-nine percent of the isolates expressed capsular polysaccharide (CP) in vitro, 58 % expressed CP5 and 11 % expressed CP8. All of the isolates harboured the icaA and icaD genes, and 95.6 % of the isolates carried the bap gene. Of the 159 isolates analysed, 97.5 % were biofilm producers. A significant association between the capsular genotype and phenotype and the amount of biofilm formation was detected: cap5/CP5 isolates tended to form more biofilm and to produce a thinner CP layer than cap8/CP8 isolates. The results indicate a high potential for pathogenicity among S. aureus isolated from bovine milk collected from three different regions in Brazil.
Fil: Salimena, Alessandra P. S.. Embrapa Dairy Cattle Research Center; Brasil. Federal University of Lavras; Brasil
Fil: Lange, Carla C.. Embrapa Dairy Cattle Research Center; Brasil
Fil: Camussone, Cecilia María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Tecnológica Nacional. Facultad Regional Rafaela; Argentina
Fil: Signorini Porchietto, Marcelo Lisandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Tecnológica Nacional. Facultad Regional Rafaela; Argentina
Fil: Calvinho, Luis Fernando. Universidad Tecnológica Nacional. Facultad Regional Rafaela; Argentina
Fil: Brito, Maria A. V. P.. Embrapa Dairy Cattle Research Center; Brasil
Fil: Borges, Cristiano A. V.. Embrapa Dairy Cattle Research Center; Brasil
Fil: Guimarães, Alessandro S.. Embrapa Dairy Cattle Research Center; Brasil
Fil: Ribeiro, João B.. Embrapa Dairy Cattle Research Center; Brasil
Fil: Mendonça, Letícia C.. Embrapa Dairy Cattle Research Center; Brasil
Fil: Piccoli, Roberta H.. Federal University of Lavras; Brasil
Materia
Adhesin
Bap
Cap
Capsule
Icaad
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/55723
Acceder
id CONICETDig_94a4f7f085b141b733ef5f39f6026c3c
oai_identifier_str oai:ri.conicet.gov.ar:11336/55723
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farmsSalimena, Alessandra P. S.Lange, Carla C.Camussone, Cecilia MaríaSignorini Porchietto, Marcelo LisandroCalvinho, Luis FernandoBrito, Maria A. V. P.Borges, Cristiano A. V.Guimarães, Alessandro S.Ribeiro, João B.Mendonça, Letícia C.Piccoli, Roberta H.AdhesinBapCapCapsuleIcaadhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Staphylococcus aureus is a pathogen that frequently causes mastitis in bovine herds worldwide. This pathogen produces several virulence factors, including cell-associated adhesins, toxic and cytolytic exoproteins, and capsular polysaccharides. The aim of the present study was to test for the presence of genes involved in capsular polysaccharide production and biofilm formation in S. aureus isolated from bovine mastitis samples collected from 119 dairy herds located in three different Brazilian regions, as well as to assay the production of capsular polysaccharides and biofilm, in vitro. The detection of the cap, icaAD, and bap genes was performed using PCR. The detection and quantification of capsular polysaccharide production was performed using ELISA assays. The ability of the isolates to form a biofilm was examined using the polystyrene surface of microtiter plates. All 159 S. aureus isolates investigated harboured the cap gene: 80 % carried the cap5 gene and 20 % carried the cap8 gene. Sixty-nine percent of the isolates expressed capsular polysaccharide (CP) in vitro, 58 % expressed CP5 and 11 % expressed CP8. All of the isolates harboured the icaA and icaD genes, and 95.6 % of the isolates carried the bap gene. Of the 159 isolates analysed, 97.5 % were biofilm producers. A significant association between the capsular genotype and phenotype and the amount of biofilm formation was detected: cap5/CP5 isolates tended to form more biofilm and to produce a thinner CP layer than cap8/CP8 isolates. The results indicate a high potential for pathogenicity among S. aureus isolated from bovine milk collected from three different regions in Brazil.Fil: Salimena, Alessandra P. S.. Embrapa Dairy Cattle Research Center; Brasil. Federal University of Lavras; BrasilFil: Lange, Carla C.. Embrapa Dairy Cattle Research Center; BrasilFil: Camussone, Cecilia María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Tecnológica Nacional. Facultad Regional Rafaela; ArgentinaFil: Signorini Porchietto, Marcelo Lisandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Tecnológica Nacional. Facultad Regional Rafaela; ArgentinaFil: Calvinho, Luis Fernando. Universidad Tecnológica Nacional. Facultad Regional Rafaela; ArgentinaFil: Brito, Maria A. V. P.. Embrapa Dairy Cattle Research Center; BrasilFil: Borges, Cristiano A. V.. Embrapa Dairy Cattle Research Center; BrasilFil: Guimarães, Alessandro S.. Embrapa Dairy Cattle Research Center; BrasilFil: Ribeiro, João B.. Embrapa Dairy Cattle Research Center; BrasilFil: Mendonça, Letícia C.. Embrapa Dairy Cattle Research Center; BrasilFil: Piccoli, Roberta H.. Federal University of Lavras; BrasilSpringer2016-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/55723Salimena, Alessandra P. S.; Lange, Carla C.; Camussone, Cecilia María; Signorini Porchietto, Marcelo Lisandro; Calvinho, Luis Fernando; et al.; Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms; Springer; Veterinary Research Communications; 40; 3-4; 12-2016; 97-1060165-7380CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11259-016-9658-5info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11259-016-9658-5info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:33:54Zoai:ri.conicet.gov.ar:11336/55723instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:33:54.881CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms
title Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms
spellingShingle Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms
Salimena, Alessandra P. S.
Adhesin
Bap
Cap
Capsule
Icaad
title_short Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms
title_full Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms
title_fullStr Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms
title_full_unstemmed Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms
title_sort Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms
dc.creator.none.fl_str_mv Salimena, Alessandra P. S.
Lange, Carla C.
Camussone, Cecilia María
Signorini Porchietto, Marcelo Lisandro
Calvinho, Luis Fernando
Brito, Maria A. V. P.
Borges, Cristiano A. V.
Guimarães, Alessandro S.
Ribeiro, João B.
Mendonça, Letícia C.
Piccoli, Roberta H.
author Salimena, Alessandra P. S.
author_facet Salimena, Alessandra P. S.
Lange, Carla C.
Camussone, Cecilia María
Signorini Porchietto, Marcelo Lisandro
Calvinho, Luis Fernando
Brito, Maria A. V. P.
Borges, Cristiano A. V.
Guimarães, Alessandro S.
Ribeiro, João B.
Mendonça, Letícia C.
Piccoli, Roberta H.
author_role author
author2 Lange, Carla C.
Camussone, Cecilia María
Signorini Porchietto, Marcelo Lisandro
Calvinho, Luis Fernando
Brito, Maria A. V. P.
Borges, Cristiano A. V.
Guimarães, Alessandro S.
Ribeiro, João B.
Mendonça, Letícia C.
Piccoli, Roberta H.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Adhesin
Bap
Cap
Capsule
Icaad
topic Adhesin
Bap
Cap
Capsule
Icaad
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Staphylococcus aureus is a pathogen that frequently causes mastitis in bovine herds worldwide. This pathogen produces several virulence factors, including cell-associated adhesins, toxic and cytolytic exoproteins, and capsular polysaccharides. The aim of the present study was to test for the presence of genes involved in capsular polysaccharide production and biofilm formation in S. aureus isolated from bovine mastitis samples collected from 119 dairy herds located in three different Brazilian regions, as well as to assay the production of capsular polysaccharides and biofilm, in vitro. The detection of the cap, icaAD, and bap genes was performed using PCR. The detection and quantification of capsular polysaccharide production was performed using ELISA assays. The ability of the isolates to form a biofilm was examined using the polystyrene surface of microtiter plates. All 159 S. aureus isolates investigated harboured the cap gene: 80 % carried the cap5 gene and 20 % carried the cap8 gene. Sixty-nine percent of the isolates expressed capsular polysaccharide (CP) in vitro, 58 % expressed CP5 and 11 % expressed CP8. All of the isolates harboured the icaA and icaD genes, and 95.6 % of the isolates carried the bap gene. Of the 159 isolates analysed, 97.5 % were biofilm producers. A significant association between the capsular genotype and phenotype and the amount of biofilm formation was detected: cap5/CP5 isolates tended to form more biofilm and to produce a thinner CP layer than cap8/CP8 isolates. The results indicate a high potential for pathogenicity among S. aureus isolated from bovine milk collected from three different regions in Brazil.
Fil: Salimena, Alessandra P. S.. Embrapa Dairy Cattle Research Center; Brasil. Federal University of Lavras; Brasil
Fil: Lange, Carla C.. Embrapa Dairy Cattle Research Center; Brasil
Fil: Camussone, Cecilia María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Tecnológica Nacional. Facultad Regional Rafaela; Argentina
Fil: Signorini Porchietto, Marcelo Lisandro. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Tecnológica Nacional. Facultad Regional Rafaela; Argentina
Fil: Calvinho, Luis Fernando. Universidad Tecnológica Nacional. Facultad Regional Rafaela; Argentina
Fil: Brito, Maria A. V. P.. Embrapa Dairy Cattle Research Center; Brasil
Fil: Borges, Cristiano A. V.. Embrapa Dairy Cattle Research Center; Brasil
Fil: Guimarães, Alessandro S.. Embrapa Dairy Cattle Research Center; Brasil
Fil: Ribeiro, João B.. Embrapa Dairy Cattle Research Center; Brasil
Fil: Mendonça, Letícia C.. Embrapa Dairy Cattle Research Center; Brasil
Fil: Piccoli, Roberta H.. Federal University of Lavras; Brasil
description Staphylococcus aureus is a pathogen that frequently causes mastitis in bovine herds worldwide. This pathogen produces several virulence factors, including cell-associated adhesins, toxic and cytolytic exoproteins, and capsular polysaccharides. The aim of the present study was to test for the presence of genes involved in capsular polysaccharide production and biofilm formation in S. aureus isolated from bovine mastitis samples collected from 119 dairy herds located in three different Brazilian regions, as well as to assay the production of capsular polysaccharides and biofilm, in vitro. The detection of the cap, icaAD, and bap genes was performed using PCR. The detection and quantification of capsular polysaccharide production was performed using ELISA assays. The ability of the isolates to form a biofilm was examined using the polystyrene surface of microtiter plates. All 159 S. aureus isolates investigated harboured the cap gene: 80 % carried the cap5 gene and 20 % carried the cap8 gene. Sixty-nine percent of the isolates expressed capsular polysaccharide (CP) in vitro, 58 % expressed CP5 and 11 % expressed CP8. All of the isolates harboured the icaA and icaD genes, and 95.6 % of the isolates carried the bap gene. Of the 159 isolates analysed, 97.5 % were biofilm producers. A significant association between the capsular genotype and phenotype and the amount of biofilm formation was detected: cap5/CP5 isolates tended to form more biofilm and to produce a thinner CP layer than cap8/CP8 isolates. The results indicate a high potential for pathogenicity among S. aureus isolated from bovine milk collected from three different regions in Brazil.
publishDate 2016
dc.date.none.fl_str_mv 2016-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/55723
Salimena, Alessandra P. S.; Lange, Carla C.; Camussone, Cecilia María; Signorini Porchietto, Marcelo Lisandro; Calvinho, Luis Fernando; et al.; Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms; Springer; Veterinary Research Communications; 40; 3-4; 12-2016; 97-106
0165-7380
CONICET Digital
CONICET
url http://hdl.handle.net/11336/55723
identifier_str_mv Salimena, Alessandra P. S.; Lange, Carla C.; Camussone, Cecilia María; Signorini Porchietto, Marcelo Lisandro; Calvinho, Luis Fernando; et al.; Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms; Springer; Veterinary Research Communications; 40; 3-4; 12-2016; 97-106
0165-7380
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s11259-016-9658-5
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11259-016-9658-5
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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