Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms

Autores
Salimena, Alessandra P.S.; Lange, Carla C.; Camussone, Cecilia; Signorini, Marcelo; Calvinho, Luis Fernando; Brito, María A.V.P.; Borges, Cristiano A.V.; Guimaraes, Alessandro S.; Ribeiro, Joao B.; Mendonca, Leticia C.; Piccoli, Roberta H.
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Staphylococcus aureus is a pathogen that frequently causes mastitis in bovine herds worldwide. This pathogen produces several virulence factors, including cell-associated adhesins, toxic and cytolytic exoproteins, and capsular polysaccharides. The aim of the present study was to test for the presence of genes involved in capsular polysaccharide production and biofilm formation in S. aureus isolated from bovine mastitis samples collected from 119 dairy herds located in three different Brazilian regions, as well as to assay the production of capsular polysaccharides and biofilm, in vitro. The detection of the cap, icaAD, and bap genes was performed using PCR. The detection and quantification of capsular polysaccharide production was performed using ELISA assays. The ability of the isolates to form a biofilm was examined using the polystyrene surface of microtiter plates. All 159 S. aureus isolates investigated harboured the cap gene: 80 % carried the cap5 gene and 20 % carried the cap8 gene. Sixty-nine percent of the isolates expressed capsular polysaccharide (CP) in vitro, 58 % expressed CP5 and 11 % expressed CP8. All of the isolates harboured the icaA and icaD genes, and 95.6 % of the isolates carried the bap gene. Of the 159 isolates analysed, 97.5 % were biofilm producers. A significant association between the capsular genotype and phenotype and the amount of biofilm formation was detected: cap5/CP5 isolates tended to form more biofilm and to produce a thinner CP layer than cap8/CP8 isolates. The results indicate a high potential for pathogenicity among S. aureus isolated from bovine milk collected from three different regions in Brazil.
EEA Rafaela
Fil: Salimena, Alessandra P.S. EMBRAPA Dairy Cattle Research Center; Brasil. Federal University of Lavras. Food Science Department; Brasil
Fil: Lange, Carla C. EMBRAPA Dairy Cattle Research Center; Brasil
Fil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Brito, María A.V.P. EMBRAPA Dairy Cattle Research Center; Brasil
Fil: Borges, Cristiano A.V. EMBRAPA Dairy Cattle Research Center; Brasil
Fil: Guimaraes, Alessandro S. EMBRAPA Dairy Cattle Research Center; Brasil
Fil: Ribeiro, Joao B. EMBRAPA Dairy Cattle Research Center; Brasil
Fil: Mendonca, Leticia C. EMBRAPA Dairy Cattle Research Center; Brasil
Fil: Piccoli, Roberta H. Federal University of Lavras. Food Science Department; Brasil
Fuente
Veterinary research communications 40 (3-4) : 97-106. (2016)
Materia
Staphylococcus aureus
Ganado de Leche
Genotipos
Fenotipos
Leche
Dairy Cattle
Genotypes
Phenotypes
Milk
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/910
Acceder
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oai_identifier_str oai:localhost:20.500.12123/910
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farmsSalimena, Alessandra P.S.Lange, Carla C.Camussone, CeciliaSignorini, MarceloCalvinho, Luis FernandoBrito, María A.V.P.Borges, Cristiano A.V.Guimaraes, Alessandro S.Ribeiro, Joao B.Mendonca, Leticia C.Piccoli, Roberta H.Staphylococcus aureusGanado de LecheGenotiposFenotiposLecheDairy CattleGenotypesPhenotypesMilkStaphylococcus aureus is a pathogen that frequently causes mastitis in bovine herds worldwide. This pathogen produces several virulence factors, including cell-associated adhesins, toxic and cytolytic exoproteins, and capsular polysaccharides. The aim of the present study was to test for the presence of genes involved in capsular polysaccharide production and biofilm formation in S. aureus isolated from bovine mastitis samples collected from 119 dairy herds located in three different Brazilian regions, as well as to assay the production of capsular polysaccharides and biofilm, in vitro. The detection of the cap, icaAD, and bap genes was performed using PCR. The detection and quantification of capsular polysaccharide production was performed using ELISA assays. The ability of the isolates to form a biofilm was examined using the polystyrene surface of microtiter plates. All 159 S. aureus isolates investigated harboured the cap gene: 80 % carried the cap5 gene and 20 % carried the cap8 gene. Sixty-nine percent of the isolates expressed capsular polysaccharide (CP) in vitro, 58 % expressed CP5 and 11 % expressed CP8. All of the isolates harboured the icaA and icaD genes, and 95.6 % of the isolates carried the bap gene. Of the 159 isolates analysed, 97.5 % were biofilm producers. A significant association between the capsular genotype and phenotype and the amount of biofilm formation was detected: cap5/CP5 isolates tended to form more biofilm and to produce a thinner CP layer than cap8/CP8 isolates. The results indicate a high potential for pathogenicity among S. aureus isolated from bovine milk collected from three different regions in Brazil.EEA RafaelaFil: Salimena, Alessandra P.S. EMBRAPA Dairy Cattle Research Center; Brasil. Federal University of Lavras. Food Science Department; BrasilFil: Lange, Carla C. EMBRAPA Dairy Cattle Research Center; BrasilFil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Brito, María A.V.P. EMBRAPA Dairy Cattle Research Center; BrasilFil: Borges, Cristiano A.V. EMBRAPA Dairy Cattle Research Center; BrasilFil: Guimaraes, Alessandro S. EMBRAPA Dairy Cattle Research Center; BrasilFil: Ribeiro, Joao B. EMBRAPA Dairy Cattle Research Center; BrasilFil: Mendonca, Leticia C. EMBRAPA Dairy Cattle Research Center; BrasilFil: Piccoli, Roberta H. Federal University of Lavras. Food Science Department; Brasil2017-08-07T13:26:11Z2017-08-07T13:26:11Z2016-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/910https://link.springer.com/article/10.1007%2Fs11259-016-9658-51573-7446https://doi.org/10.1007/s11259-016-9658-5Veterinary research communications 40 (3-4) : 97-106. (2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología AgropecuariaengBrazil (nation)info:eu-repo/semantics/restrictedAccess2025-09-29T13:44:08Zoai:localhost:20.500.12123/910instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:08.643INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms
title Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms
spellingShingle Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms
Salimena, Alessandra P.S.
Staphylococcus aureus
Ganado de Leche
Genotipos
Fenotipos
Leche
Dairy Cattle
Genotypes
Phenotypes
Milk
title_short Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms
title_full Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms
title_fullStr Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms
title_full_unstemmed Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms
title_sort Genotypic and phenotypic detection of capsular polysaccharide and biofilm formation in Staphylococcus aureus isolated from bovine milk collected from Brazilian dairy farms
dc.creator.none.fl_str_mv Salimena, Alessandra P.S.
Lange, Carla C.
Camussone, Cecilia
Signorini, Marcelo
Calvinho, Luis Fernando
Brito, María A.V.P.
Borges, Cristiano A.V.
Guimaraes, Alessandro S.
Ribeiro, Joao B.
Mendonca, Leticia C.
Piccoli, Roberta H.
author Salimena, Alessandra P.S.
author_facet Salimena, Alessandra P.S.
Lange, Carla C.
Camussone, Cecilia
Signorini, Marcelo
Calvinho, Luis Fernando
Brito, María A.V.P.
Borges, Cristiano A.V.
Guimaraes, Alessandro S.
Ribeiro, Joao B.
Mendonca, Leticia C.
Piccoli, Roberta H.
author_role author
author2 Lange, Carla C.
Camussone, Cecilia
Signorini, Marcelo
Calvinho, Luis Fernando
Brito, María A.V.P.
Borges, Cristiano A.V.
Guimaraes, Alessandro S.
Ribeiro, Joao B.
Mendonca, Leticia C.
Piccoli, Roberta H.
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Staphylococcus aureus
Ganado de Leche
Genotipos
Fenotipos
Leche
Dairy Cattle
Genotypes
Phenotypes
Milk
topic Staphylococcus aureus
Ganado de Leche
Genotipos
Fenotipos
Leche
Dairy Cattle
Genotypes
Phenotypes
Milk
dc.description.none.fl_txt_mv Staphylococcus aureus is a pathogen that frequently causes mastitis in bovine herds worldwide. This pathogen produces several virulence factors, including cell-associated adhesins, toxic and cytolytic exoproteins, and capsular polysaccharides. The aim of the present study was to test for the presence of genes involved in capsular polysaccharide production and biofilm formation in S. aureus isolated from bovine mastitis samples collected from 119 dairy herds located in three different Brazilian regions, as well as to assay the production of capsular polysaccharides and biofilm, in vitro. The detection of the cap, icaAD, and bap genes was performed using PCR. The detection and quantification of capsular polysaccharide production was performed using ELISA assays. The ability of the isolates to form a biofilm was examined using the polystyrene surface of microtiter plates. All 159 S. aureus isolates investigated harboured the cap gene: 80 % carried the cap5 gene and 20 % carried the cap8 gene. Sixty-nine percent of the isolates expressed capsular polysaccharide (CP) in vitro, 58 % expressed CP5 and 11 % expressed CP8. All of the isolates harboured the icaA and icaD genes, and 95.6 % of the isolates carried the bap gene. Of the 159 isolates analysed, 97.5 % were biofilm producers. A significant association between the capsular genotype and phenotype and the amount of biofilm formation was detected: cap5/CP5 isolates tended to form more biofilm and to produce a thinner CP layer than cap8/CP8 isolates. The results indicate a high potential for pathogenicity among S. aureus isolated from bovine milk collected from three different regions in Brazil.
EEA Rafaela
Fil: Salimena, Alessandra P.S. EMBRAPA Dairy Cattle Research Center; Brasil. Federal University of Lavras. Food Science Department; Brasil
Fil: Lange, Carla C. EMBRAPA Dairy Cattle Research Center; Brasil
Fil: Camussone, Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Signorini, Marcelo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Calvinho, Luis Fernando. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Brito, María A.V.P. EMBRAPA Dairy Cattle Research Center; Brasil
Fil: Borges, Cristiano A.V. EMBRAPA Dairy Cattle Research Center; Brasil
Fil: Guimaraes, Alessandro S. EMBRAPA Dairy Cattle Research Center; Brasil
Fil: Ribeiro, Joao B. EMBRAPA Dairy Cattle Research Center; Brasil
Fil: Mendonca, Leticia C. EMBRAPA Dairy Cattle Research Center; Brasil
Fil: Piccoli, Roberta H. Federal University of Lavras. Food Science Department; Brasil
description Staphylococcus aureus is a pathogen that frequently causes mastitis in bovine herds worldwide. This pathogen produces several virulence factors, including cell-associated adhesins, toxic and cytolytic exoproteins, and capsular polysaccharides. The aim of the present study was to test for the presence of genes involved in capsular polysaccharide production and biofilm formation in S. aureus isolated from bovine mastitis samples collected from 119 dairy herds located in three different Brazilian regions, as well as to assay the production of capsular polysaccharides and biofilm, in vitro. The detection of the cap, icaAD, and bap genes was performed using PCR. The detection and quantification of capsular polysaccharide production was performed using ELISA assays. The ability of the isolates to form a biofilm was examined using the polystyrene surface of microtiter plates. All 159 S. aureus isolates investigated harboured the cap gene: 80 % carried the cap5 gene and 20 % carried the cap8 gene. Sixty-nine percent of the isolates expressed capsular polysaccharide (CP) in vitro, 58 % expressed CP5 and 11 % expressed CP8. All of the isolates harboured the icaA and icaD genes, and 95.6 % of the isolates carried the bap gene. Of the 159 isolates analysed, 97.5 % were biofilm producers. A significant association between the capsular genotype and phenotype and the amount of biofilm formation was detected: cap5/CP5 isolates tended to form more biofilm and to produce a thinner CP layer than cap8/CP8 isolates. The results indicate a high potential for pathogenicity among S. aureus isolated from bovine milk collected from three different regions in Brazil.
publishDate 2016
dc.date.none.fl_str_mv 2016-12
2017-08-07T13:26:11Z
2017-08-07T13:26:11Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/910
https://link.springer.com/article/10.1007%2Fs11259-016-9658-5
1573-7446
https://doi.org/10.1007/s11259-016-9658-5
url http://hdl.handle.net/20.500.12123/910
https://link.springer.com/article/10.1007%2Fs11259-016-9658-5
https://doi.org/10.1007/s11259-016-9658-5
identifier_str_mv 1573-7446
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Brazil (nation)
dc.source.none.fl_str_mv Veterinary research communications 40 (3-4) : 97-106. (2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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