Kinetic studies of sodium and metforminium decavanadates decomposition and in vitro cytotoxicity and insulin-like activity

Autores
Silva Nolasco, Aniela M.; Camacho, Luz; Saavedra Díaz, Rafael Omar; Hernández Abreu, Oswaldo; Leon, Ignacio Esteban; Sánchez Lombardo, Irma
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The kinetics of the decomposition of 0.5 and 1.0 mM sodium decavanadate (NaDeca) and metforminium decavanadate (MetfDeca) solutions were studied by51V NMR in Dulbecco’s modified Eagle’s medium (DMEM) medium (pH 7.4) at 25◦C. The results showed that decomposition products are orthovanadate [H2VO4]− (V1) and metavanadate species like [H2V2O7]2− (V2), [V4O12]4− (V4) and [V5O15]5− (V5) for both compounds. The calculated half-life times of the decomposition reaction were 9 and 11 h for NaDeca and MetfDeca, respectively, at 1 mM concentration. The hydrolysis products that presented the highest rate constants were V1 and V4 for both compounds. Cytotoxic activity studies using non-tumorigenic HEK293 cell line and human liver cancer HEPG2 cells showed that decavanadates compounds exhibit selectivity action toward HEPG2 cells after 24 h. The effect of vanadium compounds (8–30 µM concentration) on the protein expression of AKT and AMPK were investigated in HEPG2 cell lines, showing that NaDeca and MetfDeca compounds exhibit a dose-dependence increase in phosphorylated AKT. Additionally, NaDeca at 30 µM concentration stimulated the glucose cell uptake moderately (62%) in 3T3-L1 adipocytes. Finally, an insulin release assay in βTC-6 cells (30 µM concentration) showed that sodium orthovanadate (MetV) and MetfDeca enhanced insulin release by 0.7 and 1-fold, respectively.
Fil: Silva Nolasco, Aniela M.. Universidad Juárez Autónoma de Tabasco; México. Instituto Nacional de Pediatría; México
Fil: Camacho, Luz. Instituto Nacional de Pediatría; México
Fil: Saavedra Díaz, Rafael Omar. Universidad Juárez Autónoma de Tabasco; México
Fil: Hernández Abreu, Oswaldo. Universidad Juárez Autónoma de Tabasco; México
Fil: Leon, Ignacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina
Fil: Sánchez Lombardo, Irma. Universidad Juárez Autónoma de Tabasco; México
Materia
CYTOTOXICITY
DECAVANADATE
DIABETES THERAPY
INSULIN-LIKE ACTIVITY
POLYOXOMETALATES
VANADIUM BIOCHEMISTRY
VANADIUM SPECIATION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/143815
Acceder
id CONICETDig_92e5561ec5e23f2c42eeecfb21a62e35
oai_identifier_str oai:ri.conicet.gov.ar:11336/143815
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Kinetic studies of sodium and metforminium decavanadates decomposition and in vitro cytotoxicity and insulin-like activitySilva Nolasco, Aniela M.Camacho, LuzSaavedra Díaz, Rafael OmarHernández Abreu, OswaldoLeon, Ignacio EstebanSánchez Lombardo, IrmaCYTOTOXICITYDECAVANADATEDIABETES THERAPYINSULIN-LIKE ACTIVITYPOLYOXOMETALATESVANADIUM BIOCHEMISTRYVANADIUM SPECIATIONhttps://purl.org/becyt/ford/1.4https://purl.org/becyt/ford/1The kinetics of the decomposition of 0.5 and 1.0 mM sodium decavanadate (NaDeca) and metforminium decavanadate (MetfDeca) solutions were studied by51V NMR in Dulbecco’s modified Eagle’s medium (DMEM) medium (pH 7.4) at 25◦C. The results showed that decomposition products are orthovanadate [H2VO4]− (V1) and metavanadate species like [H2V2O7]2− (V2), [V4O12]4− (V4) and [V5O15]5− (V5) for both compounds. The calculated half-life times of the decomposition reaction were 9 and 11 h for NaDeca and MetfDeca, respectively, at 1 mM concentration. The hydrolysis products that presented the highest rate constants were V1 and V4 for both compounds. Cytotoxic activity studies using non-tumorigenic HEK293 cell line and human liver cancer HEPG2 cells showed that decavanadates compounds exhibit selectivity action toward HEPG2 cells after 24 h. The effect of vanadium compounds (8–30 µM concentration) on the protein expression of AKT and AMPK were investigated in HEPG2 cell lines, showing that NaDeca and MetfDeca compounds exhibit a dose-dependence increase in phosphorylated AKT. Additionally, NaDeca at 30 µM concentration stimulated the glucose cell uptake moderately (62%) in 3T3-L1 adipocytes. Finally, an insulin release assay in βTC-6 cells (30 µM concentration) showed that sodium orthovanadate (MetV) and MetfDeca enhanced insulin release by 0.7 and 1-fold, respectively.Fil: Silva Nolasco, Aniela M.. Universidad Juárez Autónoma de Tabasco; México. Instituto Nacional de Pediatría; MéxicoFil: Camacho, Luz. Instituto Nacional de Pediatría; MéxicoFil: Saavedra Díaz, Rafael Omar. Universidad Juárez Autónoma de Tabasco; MéxicoFil: Hernández Abreu, Oswaldo. Universidad Juárez Autónoma de Tabasco; MéxicoFil: Leon, Ignacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; ArgentinaFil: Sánchez Lombardo, Irma. Universidad Juárez Autónoma de Tabasco; MéxicoMDPI AG2020-12-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/143815Silva Nolasco, Aniela M.; Camacho, Luz; Saavedra Díaz, Rafael Omar; Hernández Abreu, Oswaldo; Leon, Ignacio Esteban; et al.; Kinetic studies of sodium and metforminium decavanadates decomposition and in vitro cytotoxicity and insulin-like activity; MDPI AG; Inorganics; 8; 12; 08-12-2020; 1-202304-6740CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3390/inorganics8120067info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2304-6740/8/12/67info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:12:19Zoai:ri.conicet.gov.ar:11336/143815instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:12:19.621CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Kinetic studies of sodium and metforminium decavanadates decomposition and in vitro cytotoxicity and insulin-like activity
title Kinetic studies of sodium and metforminium decavanadates decomposition and in vitro cytotoxicity and insulin-like activity
spellingShingle Kinetic studies of sodium and metforminium decavanadates decomposition and in vitro cytotoxicity and insulin-like activity
Silva Nolasco, Aniela M.
CYTOTOXICITY
DECAVANADATE
DIABETES THERAPY
INSULIN-LIKE ACTIVITY
POLYOXOMETALATES
VANADIUM BIOCHEMISTRY
VANADIUM SPECIATION
title_short Kinetic studies of sodium and metforminium decavanadates decomposition and in vitro cytotoxicity and insulin-like activity
title_full Kinetic studies of sodium and metforminium decavanadates decomposition and in vitro cytotoxicity and insulin-like activity
title_fullStr Kinetic studies of sodium and metforminium decavanadates decomposition and in vitro cytotoxicity and insulin-like activity
title_full_unstemmed Kinetic studies of sodium and metforminium decavanadates decomposition and in vitro cytotoxicity and insulin-like activity
title_sort Kinetic studies of sodium and metforminium decavanadates decomposition and in vitro cytotoxicity and insulin-like activity
dc.creator.none.fl_str_mv Silva Nolasco, Aniela M.
Camacho, Luz
Saavedra Díaz, Rafael Omar
Hernández Abreu, Oswaldo
Leon, Ignacio Esteban
Sánchez Lombardo, Irma
author Silva Nolasco, Aniela M.
author_facet Silva Nolasco, Aniela M.
Camacho, Luz
Saavedra Díaz, Rafael Omar
Hernández Abreu, Oswaldo
Leon, Ignacio Esteban
Sánchez Lombardo, Irma
author_role author
author2 Camacho, Luz
Saavedra Díaz, Rafael Omar
Hernández Abreu, Oswaldo
Leon, Ignacio Esteban
Sánchez Lombardo, Irma
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv CYTOTOXICITY
DECAVANADATE
DIABETES THERAPY
INSULIN-LIKE ACTIVITY
POLYOXOMETALATES
VANADIUM BIOCHEMISTRY
VANADIUM SPECIATION
topic CYTOTOXICITY
DECAVANADATE
DIABETES THERAPY
INSULIN-LIKE ACTIVITY
POLYOXOMETALATES
VANADIUM BIOCHEMISTRY
VANADIUM SPECIATION
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.4
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The kinetics of the decomposition of 0.5 and 1.0 mM sodium decavanadate (NaDeca) and metforminium decavanadate (MetfDeca) solutions were studied by51V NMR in Dulbecco’s modified Eagle’s medium (DMEM) medium (pH 7.4) at 25◦C. The results showed that decomposition products are orthovanadate [H2VO4]− (V1) and metavanadate species like [H2V2O7]2− (V2), [V4O12]4− (V4) and [V5O15]5− (V5) for both compounds. The calculated half-life times of the decomposition reaction were 9 and 11 h for NaDeca and MetfDeca, respectively, at 1 mM concentration. The hydrolysis products that presented the highest rate constants were V1 and V4 for both compounds. Cytotoxic activity studies using non-tumorigenic HEK293 cell line and human liver cancer HEPG2 cells showed that decavanadates compounds exhibit selectivity action toward HEPG2 cells after 24 h. The effect of vanadium compounds (8–30 µM concentration) on the protein expression of AKT and AMPK were investigated in HEPG2 cell lines, showing that NaDeca and MetfDeca compounds exhibit a dose-dependence increase in phosphorylated AKT. Additionally, NaDeca at 30 µM concentration stimulated the glucose cell uptake moderately (62%) in 3T3-L1 adipocytes. Finally, an insulin release assay in βTC-6 cells (30 µM concentration) showed that sodium orthovanadate (MetV) and MetfDeca enhanced insulin release by 0.7 and 1-fold, respectively.
Fil: Silva Nolasco, Aniela M.. Universidad Juárez Autónoma de Tabasco; México. Instituto Nacional de Pediatría; México
Fil: Camacho, Luz. Instituto Nacional de Pediatría; México
Fil: Saavedra Díaz, Rafael Omar. Universidad Juárez Autónoma de Tabasco; México
Fil: Hernández Abreu, Oswaldo. Universidad Juárez Autónoma de Tabasco; México
Fil: Leon, Ignacio Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Centro de Química Inorgánica "Dr. Pedro J. Aymonino". Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Centro de Química Inorgánica "Dr. Pedro J. Aymonino"; Argentina
Fil: Sánchez Lombardo, Irma. Universidad Juárez Autónoma de Tabasco; México
description The kinetics of the decomposition of 0.5 and 1.0 mM sodium decavanadate (NaDeca) and metforminium decavanadate (MetfDeca) solutions were studied by51V NMR in Dulbecco’s modified Eagle’s medium (DMEM) medium (pH 7.4) at 25◦C. The results showed that decomposition products are orthovanadate [H2VO4]− (V1) and metavanadate species like [H2V2O7]2− (V2), [V4O12]4− (V4) and [V5O15]5− (V5) for both compounds. The calculated half-life times of the decomposition reaction were 9 and 11 h for NaDeca and MetfDeca, respectively, at 1 mM concentration. The hydrolysis products that presented the highest rate constants were V1 and V4 for both compounds. Cytotoxic activity studies using non-tumorigenic HEK293 cell line and human liver cancer HEPG2 cells showed that decavanadates compounds exhibit selectivity action toward HEPG2 cells after 24 h. The effect of vanadium compounds (8–30 µM concentration) on the protein expression of AKT and AMPK were investigated in HEPG2 cell lines, showing that NaDeca and MetfDeca compounds exhibit a dose-dependence increase in phosphorylated AKT. Additionally, NaDeca at 30 µM concentration stimulated the glucose cell uptake moderately (62%) in 3T3-L1 adipocytes. Finally, an insulin release assay in βTC-6 cells (30 µM concentration) showed that sodium orthovanadate (MetV) and MetfDeca enhanced insulin release by 0.7 and 1-fold, respectively.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/143815
Silva Nolasco, Aniela M.; Camacho, Luz; Saavedra Díaz, Rafael Omar; Hernández Abreu, Oswaldo; Leon, Ignacio Esteban; et al.; Kinetic studies of sodium and metforminium decavanadates decomposition and in vitro cytotoxicity and insulin-like activity; MDPI AG; Inorganics; 8; 12; 08-12-2020; 1-20
2304-6740
CONICET Digital
CONICET
url http://hdl.handle.net/11336/143815
identifier_str_mv Silva Nolasco, Aniela M.; Camacho, Luz; Saavedra Díaz, Rafael Omar; Hernández Abreu, Oswaldo; Leon, Ignacio Esteban; et al.; Kinetic studies of sodium and metforminium decavanadates decomposition and in vitro cytotoxicity and insulin-like activity; MDPI AG; Inorganics; 8; 12; 08-12-2020; 1-20
2304-6740
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3390/inorganics8120067
info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2304-6740/8/12/67
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI AG
publisher.none.fl_str_mv MDPI AG
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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